Journal of Biochemistry, Microbiology and Biotechnology最新文献_第8页

Cytokine Profiling as A Potential Biomarker in Diffuse Large B-Cell Lymphoma 细胞因子谱分析作为弥漫性大b细胞淋巴瘤的潜在生物标志物

Journal of Biochemistry, Microbiology and Biotechnology Pub Date : 2020-12-31 DOI: 10.54987/jobimb.v8i2.534

Nor Adzimah Johdi, Hanif Zulkhairi Mohd Said, M. M. Idris, N. Ismail, W. Fariza, W. Jamaludin, S. Wahid

{"title":"Cytokine Profiling as A Potential Biomarker in Diffuse Large B-Cell Lymphoma","authors":"Nor Adzimah Johdi, Hanif Zulkhairi Mohd Said, M. M. Idris, N. Ismail, W. Fariza, W. Jamaludin, S. Wahid","doi":"10.54987/jobimb.v8i2.534","DOIUrl":"https://doi.org/10.54987/jobimb.v8i2.534","url":null,"abstract":"Cytokines are small proteins that mediate and regulate immunity. They are involved in the pathogenesis of many diseases including cancers. The concentration of these proteins in biological fluids (serum or plasma) and tissues in diseases may suggest pathway activation that leads to inflammatory response or disease progression. Therefore, these cytokines may be useful as a tool for screening, diagnosis classification between stages of disease or surveillance for therapy. Enzyme-linked immunosorbent assays (ELISA) and bioassay have been used as a gold standard in cytokine level measurements in clinical practice. However, these methods allow only single cytokine detection at a time and ineffective for screening purposes. Hence, the innovation of multiplexing technology allows measurement of many of these soluble proteins simultaneously, thus allowing rapid, cost-effective and better efficiency by using a minute amount of sample. In this study, we explored the profiles of key inflammatory cytokines from the serum derived from diffuse large b-cell lymphoma (DLBCL, n =11) and healthy volunteers (N, n =11) using multiplexed bead-based immunoassays. We aimed to evaluate if the levels of these cytokines are significantly different in these two groups and explore the possible application of the cytokine as biomarkers in early-stage screening and/or surveillance. Our results show a significantly high level of IL-17A, IL-10 and IL-6 in DLBCL-derived serum compared to n-derived serum. These preliminary results were obtained from a small sample size and could be further validated with a larger sample size cohort to produce a panel of biomarkers for DLBCL. Our findings might be useful in developing a disease-specific panel for biomarker screening assay. This could be used for early diagnosis and/or treatment surveillance.","PeriodicalId":15132,"journal":{"name":"Journal of Biochemistry, Microbiology and Biotechnology","volume":"7 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2020-12-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"75561142","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Assessment of Coliform Bacteria as Indicator of Water Quality in Jigawa State Nigeria 大肠菌群作为尼日利亚吉加瓦州水质指标的评价

Journal of Biochemistry, Microbiology and Biotechnology Pub Date : 2020-12-29 DOI: 10.54987/jobimb.v8i2.542

Naima Suleiman Lawan, A. Yakubu, Zainab Jamil Abdulsalam, Nuhu Danladi Zandam

引用次数: 0

Biofilm Formation, Adhesion with Staphylococcus aureus Against Food Borne Pathogen: A Mathematical Modeling on the Effects of Adiantum phillippense 金黄色葡萄球菌与食源性病原菌的生物膜形成、粘附作用:牛蒡草作用的数学模型

Journal of Biochemistry, Microbiology and Biotechnology Pub Date : 2020-12-28 DOI: 10.54987/jobimb.v8i2.540

G. Uba, M. A. Ginsau, K. M. Aujara

{"title":"Biofilm Formation, Adhesion with Staphylococcus aureus Against Food Borne Pathogen: A Mathematical Modeling on the Effects of Adiantum phillippense","authors":"G. Uba, M. A. Ginsau, K. M. Aujara","doi":"10.54987/jobimb.v8i2.540","DOIUrl":"https://doi.org/10.54987/jobimb.v8i2.540","url":null,"abstract":"Biofilm formation is a process by which microorganisms irreversibly bind to and grow on a surface and create extracellular polymers that promote the formation of attachments and matrixes, resulting in a change in the organisms' phenotype in terms of growth rate and transcription of genes. A. philippense is a fern with many curative properties that is medicinally treasured. Predictive mathematical modeling approach was used to study adhesion of S. aureus with biofilm. Out of the eight different primary model, modified Gompertz best fit the effect of the plant extract on the biofilm formation and adhesion with S. aureus with the least value for RMSE, AICc and the uppermost value for adjusted R2. The parameters obtained from the modified Gompertz when compared with control and chloramphenicol were ymax 0.980 (95% C.I. 0.889 to 1.070) and 0.637 (95% C.I. 0.604 to 0.670), umax 0.185 (95% C.I. 0.120 to 0.250) and 0.183 (95% C.I. 0.141 to 0.225), lag (h) 0.180 (95% C.I. -0.764 to 1.124) and 3.343 (95% C.I. 2.933 to 3.753) respectively. A strong model to use to fit sigmoidal growth or formation curves tends to be the modified Gompertz equation. The benefit of using this function is that a constant formation rate is not assumed by the Gompertz equation. Instead, it is a model that can be used to model rates of formation (of biofilm) that change over time. \u0000Keywords","PeriodicalId":15132,"journal":{"name":"Journal of Biochemistry, Microbiology and Biotechnology","volume":"57 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2020-12-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"81519866","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 2

Formulation of Antibacterial Mouthwash from Local Herbs: A Mini-Review 本地草药抗菌漱口水的配方:综述

Journal of Biochemistry, Microbiology and Biotechnology Pub Date : 2020-12-28 DOI: 10.54987/jobimb.v8i2.536

Juman Nafea, Harisun Yaakub, M. Edbeib, K. Notarte, S. Mohammed, F. Huyop

引用次数: 2

Phytochemical Screening and Antibacterial Activity of Stem Bark of Baobab Tree (Adonsonia digitata) on some clinical isolate 猴面包树(Adonsonia digitata)茎皮临床分离物的植物化学筛选及抗菌活性研究

Journal of Biochemistry, Microbiology and Biotechnology Pub Date : 2020-12-28 DOI: 10.54987/jobimb.v8i2.539

RA Ummu Rabiâ€™u, Haruna Saâ€™idu, H. Labaran, Salau Abiola Olanrewaju, M. Muntari, S. Ibrahim

{"title":"Phytochemical Screening and Antibacterial Activity of Stem Bark of Baobab Tree (Adonsonia digitata) on some clinical isolate","authors":"RA Ummu Rabiâ€™u, Haruna Saâ€™idu, H. Labaran, Salau Abiola Olanrewaju, M. Muntari, S. Ibrahim","doi":"10.54987/jobimb.v8i2.539","DOIUrl":"https://doi.org/10.54987/jobimb.v8i2.539","url":null,"abstract":"The use of plant and their secondary metabolite for the treatment of several illnesses is gaining attention across the globe. For this reason, baobab tree was procured in order to test its effect on some bacterial isolate. Powdered stem bark of Adansonia digitata was extracted with chloroform and methanol using percolation method of extraction. The extracts were dark brown and reddish brown in color respectively with a gummy texture. The chloroform and methanolic extracts were screened from the presence of secondary metabolites using standard technique. The result of photochemical screening indicated the presence of alkaloid, flavonoid, tannin, reducing sugar and steroid in one or both the extracts. The extracts were further tested, on confirmed clinical isolates of Salmonella typhi and Staphylococcus aureus using disc diffusion method and micro â€“ broth dilution technique. The result of sensitivity test indicated that the tested clinical isolates were more sensitive to chloroform extract than the methanolic extract because larger zones of inhibitions were obtained with chloroform extract. In both methods used, the organisms were susceptible to all extracts even at the lowest concentration of 15 ïg with zones of inhibitions ranging from (8 â€“ 10 mm).","PeriodicalId":15132,"journal":{"name":"Journal of Biochemistry, Microbiology and Biotechnology","volume":"43 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2020-12-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"82535540","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 2

Electroduction on Azospirillum brasilense Sp7 and Chromobacterium violaceum 巴西偶氮螺旋菌Sp7和紫色色杆菌的电诱导

Journal of Biochemistry, Microbiology and Biotechnology Pub Date : 2020-12-28 DOI: 10.54987/jobimb.v8i2.537

W. Loke, H. Saud

引用次数: 0

Antibacterial Effect of Bitter Leave (Vernonia amygdalina) on Klebsiella pneumoniae 苦叶对肺炎克雷伯菌的抑菌作用

Journal of Biochemistry, Microbiology and Biotechnology Pub Date : 2020-12-28 DOI: 10.54987/jobimb.v8i2.538

Haruna Saâ€™idu, H. Ahmad, Salau Abiola Olanrewaju, A. Mahmoud

{"title":"Antibacterial Effect of Bitter Leave (Vernonia amygdalina) on Klebsiella pneumoniae","authors":"Haruna Saâ€™idu, H. Ahmad, Salau Abiola Olanrewaju, A. Mahmoud","doi":"10.54987/jobimb.v8i2.538","DOIUrl":"https://doi.org/10.54987/jobimb.v8i2.538","url":null,"abstract":"High rate of multi drug resistance bacteria caused by Klebsiella pneumoniae is increasing day by day, which in turn becomes medical and economic important to humans. The present study investigates the antibacterial effect of bitter leaf on Klebsiella pneumoniae. Frothing test and agar well diffusion method was used in this study. The former method indicate the presence of phytochemicals such saponin, tannin, flavonoid, alkaloid and carbohydrate in the plant while the later method indicate the potential effect of the plant extract to inhibit the growth of Klebsiella pneumoniae under different concentration ranging 2.5, 5, 10 20 mg/mL. The various zones of inhibition obtained under these concentrations were 0.0Â±0, 7Â±0.8, 11Â±0.6 and 14.00Â±1.3 mm, respectively. The highest zone of inhibition recorded was 26.00Â±2.3 mm using control experiment whereby no activity was obtained using 2.5 mg/mL plant extract. Thus, increase in the concentrations of extract was found to inhibit the growth of the bacteria under study. This research reports the potential effect of Vernonia amygdalina on the activity of K. pneumoniae.","PeriodicalId":15132,"journal":{"name":"Journal of Biochemistry, Microbiology and Biotechnology","volume":"105 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2020-12-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"90343481","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Modelling the Growth of Enterobacter sp. on Polyethylene 肠杆菌在聚乙烯上生长的模拟

Journal of Biochemistry, Microbiology and Biotechnology Pub Date : 2020-07-31 DOI: 10.54987/jobimb.v8i1.508

Salihu Yahuza, Bilal Ibrahim Dan-Iya, I. Sabo

引用次数: 5

Assessment of Cereals for Fungal Contaminants and Presence of Aflatoxins from Two Major Markets in Kaduna Metropolis 卡杜纳大都会两个主要市场谷物真菌污染物和黄曲霉毒素含量的评估

Journal of Biochemistry, Microbiology and Biotechnology Pub Date : 2020-07-31 DOI: 10.54987/jobimb.v8i1.504

Idris Abdulrahman, Amina Aliyu Danbaba, G. Uba

{"title":"Assessment of Cereals for Fungal Contaminants and Presence of Aflatoxins from Two Major Markets in Kaduna Metropolis","authors":"Idris Abdulrahman, Amina Aliyu Danbaba, G. Uba","doi":"10.54987/jobimb.v8i1.504","DOIUrl":"https://doi.org/10.54987/jobimb.v8i1.504","url":null,"abstract":"Aflatoxins are mycotoxins produced by fungi of the genus Aspergillus that contaminate food products such as cereals leading to serious health and economic consequences. In order to assess the level of fungal contaminants and the presence of aflatoxins in such products, five (5) different cereal samples comprising of rice, maize, millet, wheat and sorghum were purchased each from two major markets within Kaduna metropolis. The samples were assessed for fungal contaminants using ten-fold serial dilution and cultured appropriate dilutions using pour-plate technique. Aflatoxins were detected using Enzyme-linked Immunosorbent Assay (ELISA). The results obtained revealed viable fungal counts ranging from 3 x 102 to 3.2 x 103 cfu/g. The fungal contaminants identified from the samples include Aspergillus flavus, Aspergillus niger, and Aspergillus fumigatus. The aflatoxins detected from the samples were within the range of 0.3 to 0.6 part per billion (ppb) which is less than the maximum value (10ppb) recommended by regulatory agencies such as NAFDAC. This indicates that the level of aflatoxin in the samples has insignificant risks to consumers. However, the fungal contamination is an indication of high-level contamination of the cereals, the key producers of aflatoxins. The contamination of such products by fungi should be a source of worry and necessitates the need for proper personal and environmental hygiene in the processing of cereals.","PeriodicalId":15132,"journal":{"name":"Journal of Biochemistry, Microbiology and Biotechnology","volume":"77 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2020-07-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"83321357","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Utilization of Comprehensive Mathematical Modelling to Evaluate the Growth Kinetics of Pseudomonas putida LY1 on Phenol 利用综合数学模型评价恶臭假单胞菌LY1对苯酚的生长动力学

Journal of Biochemistry, Microbiology and Biotechnology Pub Date : 2020-07-31 DOI: 10.54987/jobimb.v8i1.507

Abubakar Aisami, M. M. Usman

{"title":"Utilization of Comprehensive Mathematical Modelling to Evaluate the Growth Kinetics of Pseudomonas putida LY1 on Phenol","authors":"Abubakar Aisami, M. M. Usman","doi":"10.54987/jobimb.v8i1.507","DOIUrl":"https://doi.org/10.54987/jobimb.v8i1.507","url":null,"abstract":"Kinetic equations, which explain the behaviour of a microbe or an enzyme towards a specific substrate, are key to understanding many phenomena in biotechnological processes. They facilitate the mathematical prediction of growth parameters essential for the identification of key growth control parameters. We remodelled Banerjee and Ghoshal's published research (Banerjee and Ghoshal 2010) using some more kinetic growth models, such as Monod, Teissier, Andrews and Noack, Hinshelwood, Moser, Aiba, Webb (Edward), Yano and Koga, Han and Levenspiel and Luong used statistical methods such as Root Mean Square (RMSE), Adjusted Coefficient of Determination ( R2), corrected Akaike Information Criterion (AICc), Bias Factor, Accuracy Factor to determine the accuracy of the fitted model. The best model was Haldane with the true value of ïmax determined as the value where the gradient for the slope is zero was 0.115 h-1 at 51 mg/L phenol. The results indicate that the exhaustive use of mathematical models on available published results could gleam new optimal models that can provide new knowledge on the way toxic substance inhibits growth rate in microbes.","PeriodicalId":15132,"journal":{"name":"Journal of Biochemistry, Microbiology and Biotechnology","volume":"6 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2020-07-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"87438758","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1