Journal of Biochemistry, Microbiology and Biotechnology最新文献_第10页

Efficacy of Different Marination Methods on Microbial Quality of Meat 不同腌制方法对肉类微生物品质的影响

Journal of Biochemistry, Microbiology and Biotechnology Pub Date : 2019-12-26 DOI: 10.54987/jobimb.v7i2.481

Shehab E. Talat, Fatma H. M. Ali, Abdelrahim H. A. Hassan

{"title":"Efficacy of Different Marination Methods on Microbial Quality of Meat","authors":"Shehab E. Talat, Fatma H. M. Ali, Abdelrahim H. A. Hassan","doi":"10.54987/jobimb.v7i2.481","DOIUrl":"https://doi.org/10.54987/jobimb.v7i2.481","url":null,"abstract":"Beef meat is an animal flesh food that is considered a good diet for consumption as it contains many minerals, vitamins, basic amino acids and essential fatty acids. The people prefer marinated beef meat from a few decades. Microbiological count affection food and especially pathogenic organisms of public health importance. The tenderness and flavour considered as the main factors that affect consumerâ€™s acceptability and satisfaction. Worldâ€™s beef meat production in 2016 is approximately about 321,000,000 tons. The importance of post marinated beef meat on quality and acceptability with the importance of consumer point of view as the health and diet increases as beef meat is a high source of minerals, protein, vitamins as well as some essential compounds. Samples from meat food were collected from supermarkets, Egypt. This study evaluates the effects of marinade on meat microbial quality as reduction of the microbiological count. Microbial count was carried out by using selective medium and to determine the best marinating method. The results indicated that SDS+1% give best microbial controlling buffer where decreasing bacterial and fungal count to around zero that gave marvellous results.","PeriodicalId":15132,"journal":{"name":"Journal of Biochemistry, Microbiology and Biotechnology","volume":"6 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2019-12-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"76958667","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Molecular Identification, Phylogenetic Classification and Proteolytic Capacity of Cultivable Bacteria Isolated from Soils in Brazzaville, Republic of Congo 刚果共和国布拉柴维尔土壤中可培养细菌的分子鉴定、系统发育分类和蛋白质水解能力

Journal of Biochemistry, Microbiology and Biotechnology Pub Date : 2019-12-26 DOI: 10.54987/jobimb.v7i2.475

Ngo Itsouhou, Nguimbi Etienne, Kayath Aimé Christian, Ampa Raoul

{"title":"Molecular Identification, Phylogenetic Classification and Proteolytic Capacity of Cultivable Bacteria Isolated from Soils in Brazzaville, Republic of Congo","authors":"Ngo Itsouhou, Nguimbi Etienne, Kayath Aimé Christian, Ampa Raoul","doi":"10.54987/jobimb.v7i2.475","DOIUrl":"https://doi.org/10.54987/jobimb.v7i2.475","url":null,"abstract":"Soils present a biodiversity of bacteria, part of this microflora is cultivable, it can be estimated, characterized and identified. Four zones each comprising three sites were sampled in Brazzaville in the Republic of Congo. Phenotypic characterization, molecular identification and proteolytic capacity of soil bacteria were conducted. One of the factors, the pH was measured, the pH values were between 6 and 7. From the classical microbiology techniques, we counted the total flora of the microorganisms in (CFU / g.103), it is between 47 Â± 11.2 and 214 Â± 58.2 and that of bacteria of the genus Bacillus from 51.3 Â± 5.0 to 74 Â± 30.7. Cocci and bacilli were obtained, Gram- and Gram + bacteria distinguished, all bacteria were catalase +, some had sporulation, others not. Thirty-one (31) isolates were phenotypically characterized and 16S rDNA PCR was performed. Ten (10) strains were sequenced and the phylogenetic classification of the identified strains presented. Blastn's research on the 16S rDNA sequences of the different strains shows similarity rates (96.77% -100%) and E. value (9.00E-93 -0.0), these two indices allowed the identification of the strains studied. The percentages of the identified strains are as follows: Bacillus cereus (20%) = MN6 and MN14, Bacillus pumilus (10%) = MN7, Bacillus thuringiensis (10%) = MN12, B sp. (10%) = MN17, Bacillus subtilis (10%) = MN26, Staphylococcus haemolyticus (10%) = MN8, Staphylococcus saprophyticus (10%) = MN21, Staphylococcus sp. (10%) = MN22, Staphylococcus gallinarum (10%) = MN24. The phylogenetic tree shows that the bacteria belong to two main monophyletic genera, the genus Bacillus and the genus Staphylococcus. The proteolytic capacity of Bacillus bacteria was assessed in parallel with growth. The optical density was between (0.8- 0.93) and proteolytic enzyme production between (9-20mm). The parallelism between growth and enzymatic production shows that the two phenomena are distinct.","PeriodicalId":15132,"journal":{"name":"Journal of Biochemistry, Microbiology and Biotechnology","volume":"1 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2019-12-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"82282019","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 3

Test of Randomness of Residuals for the Four-parameter Logistic model used in Obtaining the IC50 Value for Allivum sativum Methanolic Extract Against Aeromonas hydrophila 大蒜甲醇提取物对嗜水气单胞菌IC50值的四参数Logistic模型残差随机性检验

Journal of Biochemistry, Microbiology and Biotechnology Pub Date : 2019-12-26 DOI: 10.54987/jobimb.v7i2.479

Rusnam, B. Gunasekaran, M. K. Sabullah

引用次数: 0

pH Shift Solubilization and Precipitation Protein Extraction from the Wastes of the Threadfin Bream, Nemipterus japonicus 日本刺鱼废弃物pH漂移增溶及沉淀蛋白提取研究

Journal of Biochemistry, Microbiology and Biotechnology Pub Date : 2019-07-31 DOI: 10.54987/jobimb.v7i1.446

N. Raihan, S. Abbasiliasi, J. Tan, M. Masomian, T. Ibrahim, A. Ariff, S. Mustafa

{"title":"pH Shift Solubilization and Precipitation Protein Extraction from the Wastes of the Threadfin Bream, Nemipterus japonicus","authors":"N. Raihan, S. Abbasiliasi, J. Tan, M. Masomian, T. Ibrahim, A. Ariff, S. Mustafa","doi":"10.54987/jobimb.v7i1.446","DOIUrl":"https://doi.org/10.54987/jobimb.v7i1.446","url":null,"abstract":"This study aimed at extraction of protein from the wastes of the threadfin bream, Nemipterus japonicus using pH shift solubilization and precipitation processes and evaluation of the influencing factors on protein extraction. Towards this objective, the wastes of head, skin and internal organs were collected, and their nutritional composition evaluated. Furthermore, the ratio of waste: water for protein extraction was screened and the effect of pH, centrifugation speed and time on protein solubility rate, the amino acid composition and the molecular weight of proteins via SDS-PAGE were determined. Results demonstrated that moisture was the highest percentage (~70-78%) of all wastes. Highest protein content (19.67Â±1.10%), fat content (1.81Â±0.09%) and carbohydrate content (4.43Â±0.23%) were observed in skin, head and internal organs, respectively. The optimum ratio of waste: water for protein extraction is 1:9 for head, 1:8 for internal organs and 1:6 for skin. The protein extraction efficiency for all three samples were high at pH 3 and pH 12. Protein solubility increased with increased centrifugation speed up to 10,000 Ã—g, and there was no significant difference (P>0.05) between the protein solubility at 10,000 Ã—g and 20â€™000 Ã—g. Furthermore, there was significant difference (P<0.05) between protein solubility with increased centrifugation time. Alkaline process showed a remarkably higher amino acid content as compared to that of acid version. Glutamic acid and lysine were found higher compared to other amino acids. The molecular weight of proteins isolated in this study were low (<100 KDa).","PeriodicalId":15132,"journal":{"name":"Journal of Biochemistry, Microbiology and Biotechnology","volume":"6 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2019-07-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"73285218","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 3

PASD1 Expression in Malaysian Hematological Malignancies Patients PASD1在马来西亚血液恶性肿瘤患者中的表达

Journal of Biochemistry, Microbiology and Biotechnology Pub Date : 2019-07-31 DOI: 10.54987/jobimb.v7i1.444

Nor Adzimah Johdi, Hanif Zulkhairi Mohd Said, R. Jamal, M. R. Mohd Idris, N. Ismail, W. F. Wan Jamaludin, S. F. Abd Wahid, Muaatamarulain Mustangin, Nur Maya Sabrina Tizen Laim

{"title":"PASD1 Expression in Malaysian Hematological Malignancies Patients","authors":"Nor Adzimah Johdi, Hanif Zulkhairi Mohd Said, R. Jamal, M. R. Mohd Idris, N. Ismail, W. F. Wan Jamaludin, S. F. Abd Wahid, Muaatamarulain Mustangin, Nur Maya Sabrina Tizen Laim","doi":"10.54987/jobimb.v7i1.444","DOIUrl":"https://doi.org/10.54987/jobimb.v7i1.444","url":null,"abstract":"Per ARNT SIM domain containing 1 (PASD1) protein belongs to the Cancer Testis Antigen (CTA) family. It shows restricted expression in normal tissues but are highly expressed in cancer tissues. This study aims to further investigate PASD1 expression in Malaysian hematological malignancies patients as a potential biomarker for disease progression and vaccine development. Formalin-fixed Paraffin-Embedded (FFPE) tissue specimens from hematological malignancies patients were labeled with anti-PASD1-1 and anti-PASD1-2 antibodies using immunohistochemistry method. Our results show that among DLBCL patients, 7 samples were positive for PASD1-1, 2 samples were positive for PASD1-2 while 3 samples were positive for both of the antibodies. In addition, only 1 sample from T-cell Lymphoblastic Lymphoma (TLL) showed positive staining with PASD1-1. Other classes of hematological malignancies did not show any positive staining with either antibody. Antibody PASD1-1 stained the membrane and cytoplasm of the tumor cells strongly. Moderate nuclear labeling was also observed in some cases of DLBCL with PASD1-2. PASD1-1 staining was more frequent compared to PASD1-2 in most of the samples. Positive PASD1 staining was observed in patients with age range between 36-71 years old, higher in male cases than female by 54% and higher in Malay patients compared to Chinese by 77%. Due to its frequency, the PASD1_v1 protein may play a role in the DLBCL initiation and progression. A higher number of PASD1 staining in DLBCL samples compared to other lymphoma subtypes may suggest that PASD1 may represent a potential for DLBCL subtyping marker.","PeriodicalId":15132,"journal":{"name":"Journal of Biochemistry, Microbiology and Biotechnology","volume":"34 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2019-07-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"91293568","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Encapsulation of Lactobacillus plantarum with Mannan and Sodium Alginate Improves its Cell Production 甘露聚糖和海藻酸钠包封植物乳杆菌提高其细胞产量

Journal of Biochemistry, Microbiology and Biotechnology Pub Date : 2019-07-31 DOI: 10.54987/jobimb.v7i1.447

Muhamad Amin Jahari, S. Mustafa, Y. A. Manap, Dhilia Udie Lamasudin, M. Roslan

{"title":"Encapsulation of Lactobacillus plantarum with Mannan and Sodium Alginate Improves its Cell Production","authors":"Muhamad Amin Jahari, S. Mustafa, Y. A. Manap, Dhilia Udie Lamasudin, M. Roslan","doi":"10.54987/jobimb.v7i1.447","DOIUrl":"https://doi.org/10.54987/jobimb.v7i1.447","url":null,"abstract":"Probiotic bacteria are utilized in aquaculture as they exert a crucial function in promoting and maintaining the fish health. Probiotic strains should be present in a viable form during consumption and throughout the gastrointestinal tract for maximum health benefits. Many reports stated that there is poor survival of probiotic in products containing free probiotic cells. Providing probiotic living cells with physical barrier to resist adverse environmental conditions is therefore an approach currently receiving considerable interest. In this study, Lactobacillus plantarum was encapsulated with mannan and sodium alginate to increase probiotic viability. Response surface methodology (RSM) based on Box-Behnken design was used to optimize the encapsulation process with mannan concentration (5 to 30 % w/v) and sodium alginate concentration (1 to 5 % w/v) as the independent variables evaluated. According to the regression coefficients and significance of the polynomial model, the optimum encapsulation parameters were as follows: 24.73 % w/v mannan; 1.6 % w/v sodium alginate. Under these conditions of encapsulation, the total cell production of the Lactobacillus plantarum was increased to 5.3 (108 CFU/g) as compared to the free cell culture, 3.2 (108 CFU/g).","PeriodicalId":15132,"journal":{"name":"Journal of Biochemistry, Microbiology and Biotechnology","volume":"22 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2019-07-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"87600862","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 6

Growth Characterization of Bacillus amyloliquefaciens strain KIK-12 on SDS 解淀粉芽孢杆菌KIK-12在SDS上的生长特性

Journal of Biochemistry, Microbiology and Biotechnology Pub Date : 2019-07-31 DOI: 10.54987/jobimb.v7i1.449

K. I. Karamba, H. Yakasai

引用次数: 2

Kinetics of Cyanide Degradation by Azotobacter vinelandii 维兰氏固氮菌降解氰化物动力学研究

Journal of Biochemistry, Microbiology and Biotechnology Pub Date : 2019-07-31 DOI: 10.54987/jobimb.v7i1.453

M. Manogaran, H. Yakasai, K. I. Karamba

{"title":"Kinetics of Cyanide Degradation by Azotobacter vinelandii","authors":"M. Manogaran, H. Yakasai, K. I. Karamba","doi":"10.54987/jobimb.v7i1.453","DOIUrl":"https://doi.org/10.54987/jobimb.v7i1.453","url":null,"abstract":"Cyanide is extremely toxic to living organisms. It is utilized in industries for gold and silver extraction, pharmaceuticals, plastic processing, electroplating, and agricultural chemistry. Cyanide can persevere for a long time in the soil and its bio-degradation is the best economical practice. A formerly isolated cyanide-degrading bacterium Azotobacter vinelandii exhibited substrate inhibition to degradation rate. Significant degradation inhibition constants were achieved reliably by means of non-linear regression modeling of the degradation rate profile utilizing models for substrate inhibition like Haldane, Teissier-Edward, Monod, Yano and Koga, Luong, Edward (Webb) Luong and Aiba models. Aiba model was selected as the top model established on statistical assessments like root mean square error, adjusted coefficient of determination, bias factor and accuracy factor. The calculated values for the Aiba constants qmax (the maximum specific substrate degradation rate (hâˆ’1), Ks (concentration of substrate at the half maximal degradation rate (mM) and Ki (inhibition constant (mM)) were 0.060 (95% CI, 0.024 to 0.096), 0.302 (95% CI, 0.381 to 0.222) and 0.953 (95% CI, 0.568 to 1.338), respectively. The novel constants gotten from the modeling application can be valuable for advanced secondary modeling implicating the influence of media conditions and some other dynamics on cyanide biodegradation by this bacterium.","PeriodicalId":15132,"journal":{"name":"Journal of Biochemistry, Microbiology and Biotechnology","volume":"21 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2019-07-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"78158676","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Screening of Xenobiotics-degrading Microorganisms from Sumateraâ€™s Soil 苏门答兰州土壤中外源降解微生物的筛选

Journal of Biochemistry, Microbiology and Biotechnology Pub Date : 2019-07-31 DOI: 10.54987/jobimb.v7i1.448

Rusnam, Syafrawati, N. Gusmanizar

引用次数: 0

Glycosaminoglycans From Plant Sources and The Potential Uses as Anticoagulant and Anticancer Agents 植物来源的糖胺聚糖及其作为抗凝血剂和抗癌剂的潜在用途

Journal of Biochemistry, Microbiology and Biotechnology Pub Date : 2019-07-31 DOI: 10.54987/jobimb.v7i1.455

Che Nur Mazadillina Che Zahari, N. S. Azmi, Muhammad Zaki Ramli

引用次数: 0