Journal of Babol University of Medical Sciences最新文献_第5页

A Case Report of Abnormal Branched Right Coronary Artery from Left Sinus of Valsalva 右冠状动脉左窦分支异常1例

Journal of Babol University of Medical Sciences Pub Date : 2018-07-10 DOI: 10.18869/ACADPUB.JBUMS.20.7.71

E. Nasiri, R. Gazor, S. Fathi

引用次数: 0

Immunization Evaluation of Type III Secretion System Recombinant Antigens and Shiga Like Toxin Binding Subunit of E. coli O157:H7 大肠杆菌O157:H7 III型分泌系统重组抗原与志贺样毒素结合亚基的免疫评价

Journal of Babol University of Medical Sciences Pub Date : 2018-07-10 DOI: 10.18869/ACADPUB.JBUMS.20.7.47

M. Theri, S. Nazarian, F. Ebrahimi, Mostafa Bakhshi, Javad Fathi

{"title":"Immunization Evaluation of Type III Secretion System Recombinant Antigens and Shiga Like Toxin Binding Subunit of E. coli O157:H7","authors":"M. Theri, S. Nazarian, F. Ebrahimi, Mostafa Bakhshi, Javad Fathi","doi":"10.18869/ACADPUB.JBUMS.20.7.47","DOIUrl":"https://doi.org/10.18869/ACADPUB.JBUMS.20.7.47","url":null,"abstract":"J Babol Univ Med Sci; 20(7); July 2018; PP: 47-54 Received: Nov 14 2017, Revised: Mar 14 2018, Accepted: Mar 26 2018. ABSTRACT BACKGROUND AND OBJECTIVE: Escherichia coli enterohemorrhagic (EHEC) causes diarrhea, hemolysis colitis, and uremic hemolytic syndrome. Due to the dangers of antibiotic treatment in the EHEC O157: H7 infection, vaccines offer a promising way to prevent infection. The purpose of this study was to investigate the immunity of recombinant proteins Intimin, EspA and Stx2b against infection of E. coli O157 H7. METHODS: In this research study, expression of recombinant Intimin, EspA and Stx2b proteins E.coli BL21 DE3 was induced with IPTG. Recombinant proteins were examined by Western Blot and purified using the affinity Ni-NTA chromatography. Subcutaneous and intraperitoneally immunization of mice was performed and the antibody titer was determined by ELISA. The mice were orally infected with bacteria and the bacterial loss and mortality of the mice were examined. FINDINGS: SDS-PAGE analysis showed expression of proteins with 11,17 and 34 kDa molecular weight. The recombinant proteins were confirmed by Western Blot. ELISA end point titer for humoral response to EspA, Intimin and Stx2b was 25600. The loss of bacteria in mice was 10 cfu / ml and mortality rate were reduced to 60 Percent. CONCLUSION: The results showed that immune response against EspA, Intimin and Stx2b proteins protects animals against challenges using E. coli O157: H7.","PeriodicalId":15108,"journal":{"name":"Journal of Babol University of Medical Sciences","volume":"18 1","pages":"47-54"},"PeriodicalIF":0.0,"publicationDate":"2018-07-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"83771988","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 8

Prevalence of Musculoskeletal Pains and the Demographic Factors Related to the Pain Locations in Patients with Chronic Trauma 慢性创伤患者肌肉骨骼疼痛的患病率及与疼痛部位相关的人口统计学因素

Journal of Babol University of Medical Sciences Pub Date : 2018-07-10 DOI: 10.18869/ACADPUB.JBUMS.20.7.21

AJ Mozafari Comeshtappeh, M. Feridoni, M. Falsafi, SM Esmaeilnejad Ganji

引用次数: 1

Investigation of Mutations of Exon 11-A of BRCA1 Gene in Women with Breast Cancer in the Northwest of Iran 伊朗西北部女性乳腺癌患者BRCA1基因外显子11-A突变的研究

Journal of Babol University of Medical Sciences Pub Date : 2018-07-10 DOI: 10.18869/ACADPUB.JBUMS.20.7.28

M. Hosseinpourfeizi, S. Dianati, A. Samir, Narges Dastmalchi, N. Pouladi

引用次数: 0

The Preventive/Protective Effect of Testosterone on Haloperidol-Induced Extrapyramidal Disorders in Male Rats 睾酮对氟哌啶醇诱导的雄性大鼠锥体外系疾病的预防/保护作用

Journal of Babol University of Medical Sciences Pub Date : 2018-07-10 DOI: 10.18869/ACADPUB.JBUMS.20.7.55

S. miahmadi, M. Sabahi, Rasool Haddadi

{"title":"The Preventive/Protective Effect of Testosterone on Haloperidol-Induced Extrapyramidal Disorders in Male Rats","authors":"S. miahmadi, M. Sabahi, Rasool Haddadi","doi":"10.18869/ACADPUB.JBUMS.20.7.55","DOIUrl":"https://doi.org/10.18869/ACADPUB.JBUMS.20.7.55","url":null,"abstract":"BACKGROUND AND OBJECTIVE: Many non-motor symptoms of Parkinson's disease such as depression are associated with testosterone deficiency. On the other hand, the effect of testosterone therapy on the motor symptoms of Parkinson's disease is unknown. Therefore, the present study was conducted to evaluate the effect of testosterone on haloperidol-induced extrapyramidal disorders. METHODS: In this experimental study, 60 male Wistar rats weighing 180–220 g were randomly divided into 10 groups of 6. Rats were intraperitoneally pretreated with saline, polyethylene glycol, flutamide (10 mg/kg) or testosterone (1 mg/kg) for 1 or 7 consecutive days and before administration of haloperidol. The effects of testosterone and flutamide on catalepsy and motor disorder caused by haloperidol were measured by bar test and rotarod test, respectively. FINDINGS: Haloperidol (1 mg/kg) significantly (p<0.004) resulted in catalepsy (170±17.25) and balance impairment (52±8). Pretreatment with testosterone for 7 days had a preventive effect on haloperidol-induced extrapyramidal disorders and improved catalepsy (55±8) (p<0.001) and balance impairment (178±8) (p<0.05) in mice, whereas co-administration of flutamide prevented the ameliorative effects of testosterone on extrapyramidal disorders. CONCLUSION: The results of the study showed that pretreatment with testosterone has a significant effect on the improvement of catalepsy. © 2018, Babol University of Medical Sciences. All rights reserved.","PeriodicalId":15108,"journal":{"name":"Journal of Babol University of Medical Sciences","volume":"87 1","pages":"55-62"},"PeriodicalIF":0.0,"publicationDate":"2018-07-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"81203711","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 4

The comparison of N-acetylcysteine and Vitamin D on oxidative Liver Toxicity in Sub-Acute Paraquat Poisoning n -乙酰半胱氨酸和维生素D对亚急性百草枯中毒氧化性肝毒性的比较

Journal of Babol University of Medical Sciences Pub Date : 2018-07-10 DOI: 10.18869/acadpub.jbums.20.7.14

Gh Rahmani, S. Soleimani, A. Abadi, A. Larki, A. Ranjbar

{"title":"The comparison of N-acetylcysteine and Vitamin D on oxidative Liver Toxicity in Sub-Acute Paraquat Poisoning","authors":"Gh Rahmani, S. Soleimani, A. Abadi, A. Larki, A. Ranjbar","doi":"10.18869/acadpub.jbums.20.7.14","DOIUrl":"https://doi.org/10.18869/acadpub.jbums.20.7.14","url":null,"abstract":"BACKGROUND AND OBJECTIVE: Paraquat is mainly used as an herbicide and its toxicity can be caused by acute and chronic liver disease by increasing the active oxygen species, in this study, we investigated the effect of N-acetyl cysteine and vitamin D on the oxidative toxicity of Liver tissue in sub-acute toxicity with paraquat. METHODS: In this experimental study, 36 male albino Wistar 8 weeks old were randomly divided into 6 groups: 1) control group; 2, 3) control animals received intraperitoneal injection of Vit D 2 µg/kg/day or NAC 6.25 mg/kg/day (IP); 4) received paraquat 5 mg/kg/day (IP); 5 and 6) received paraquat and treated with Vit D 2 µg/kg/day or NAC 6.25 mg/kg/day for 7 days. At the end of the study, serum and liver tissue were collected. Total antioxidant capacity (TAC), lipid peroxidation (LPO), total thiol groups (TTG), ALT and AST levels in liver tissue were evaluated. Also liver histopathological evaluation was performed. FINDING: Based on the results, in paraquat-poisoned groups compared to the healthy control group, the LPO was increased by 239.83±70.87 versus the control group 123.1±16.63 and the liver enzymes were increased. TAC in paraquatpoisoned groups was 68.66±23.41 that showed a significant decrease compared to the control group 126.50±18.25. Also, TTG in paraquat-poisoned groups was 40.50±5.95 that significantly decreased compared to control group (P<0.05). Moreover, LPO was significantly reduced in vitamin-treated groups 156.33±39.40 compared to the paraquat-poisoned group of 239.83±70.87. In addition, histopathologic findings showed that vitamin D could improve the tissue damage caused by paraquat in the liver. CONCLUSION: The results of this study indicated that Vit D and NAC decreased oxidative toxic stress, and tissue damage in the Liver caused by paraquat poisoning. © 2018, Babol University of Medical Sciences. All rights reserved.","PeriodicalId":15108,"journal":{"name":"Journal of Babol University of Medical Sciences","volume":"25 1","pages":"14-20"},"PeriodicalIF":0.0,"publicationDate":"2018-07-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"84019986","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A Review of the Indices of Mizaj-e-Meda (Temperament of Stomach) identification in Persian Medicine 波斯医学胃律鉴定指标综述

Journal of Babol University of Medical Sciences Pub Date : 2018-07-10 DOI: 10.18869/ACADPUB.JBUMS.20.7.63

E. Parsa, M. Mojahedi, M. C. Raghimi, R. Ilkhani, A. Zareiyan, R. Mokaberinejad, M. A. Vaghasloo, Mahmood Khodadoost

引用次数: 6

T375c Mutation of FGFR3 Gene in Bladder Epithelial Cells of Patients with Bladder Cancer 膀胱癌患者膀胱上皮细胞FGFR3基因T375c突变

Journal of Babol University of Medical Sciences Pub Date : 2018-07-10 DOI: 10.18869/ACADPUB.JBUMS.20.7.33

M. Pitka, H. Shafi, A. Nazemi

引用次数: 0

Inhaled Beclomethasone with or whiteout Montelukast in the Management of Pediatric Persistent Asthma 吸入倍氯米松联合或涂抹孟鲁司特治疗儿童持续性哮喘

Journal of Babol University of Medical Sciences Pub Date : 2018-07-10 DOI: 10.18869/ACADPUB.JBUMS.20.7.7

I. Mohammadzadeh, A. Modanloo, R. Alizadeh-Navaei

引用次数: 0

Effect of Isolated Tricin from Arial Part of Allium atrovoilaceum Boiss. on Proliferation and Apoptosis in PC3 Cell Line 从葱Arial部分分离Tricin的效果。PC3细胞株增殖与凋亡的研究

Journal of Babol University of Medical Sciences Pub Date : 2018-06-15 DOI: 10.18869/ACADPUB.JBUMS.20.6.36

Z. Lorigooini, S. Ghasemi, N. Dehkordi, M. Motamedi

{"title":"Effect of Isolated Tricin from Arial Part of Allium atrovoilaceum Boiss. on Proliferation and Apoptosis in PC3 Cell Line","authors":"Z. Lorigooini, S. Ghasemi, N. Dehkordi, M. Motamedi","doi":"10.18869/ACADPUB.JBUMS.20.6.36","DOIUrl":"https://doi.org/10.18869/ACADPUB.JBUMS.20.6.36","url":null,"abstract":"BACKGROUND AND OBJECTIVE: Tricin as a flavone compound, in many food sources, has anti-inflammatory and anti-proliferative effects in several cancer cell lines. Considering the importance of using natural anti-cancer drugs in treatment-resistant cancers such as prostate cancer, the aim of this study was to investigate the effect of tricin on cell growth and proliferation and induction of apoptosis in human PC3 prostate cancer cell line. \u0000METHODS: In this experimental study, the PC3 cell line was prepared from the Pasteur Institute of Iran and cultivated. Extraction and purification of Tricin were performed using column chromatography and re-crystallization of Alliuum atrovoilaceum extract. The cytotoxic effect of Tricin in concentrations of 60,80,100,120, and 140 μM was evaluated by MTT method. Apoptotic effect was evaluated in the treated cell group with the IC50 concentration of Tricin and untreated cells (control group), using an Annexin-V kit and flow cytometry. \u0000FINDINGS: The viability of cells at different tricin concentrations were 85.66±1.52, 76±3.60, 66.33±4.16, 44±3.60, and 36.66±3.21, respectively (p<0.01). The IC50 concentration of tricin was 117.5±4.4 μM for PC3 cell line. The apoptosis rate in PC3 cells after 48 hours of treatment with IC50 concentration of tricin was 24.3±0.58%, which was not significant in comparison with control cells (23.3±0.58%). \u0000CONCLUSION: The results of this study showed that Tricin resulted in cell death in the PC3 cell line, but the cell death mechanism was not apoptosis.","PeriodicalId":15108,"journal":{"name":"Journal of Babol University of Medical Sciences","volume":"27 1","pages":"36-40"},"PeriodicalIF":0.0,"publicationDate":"2018-06-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"82574542","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 2