journal of biopesticides最新文献

{"title":"BIOINSECTICIDE ACTIVITY OF SILVER NANOPARTICLES PRODUCT OF BIOSYNTHESIS USING ENTOMOPATHOGEN AGAINST THE BEET CATERPILLAR SPODOPTERA EXIGUA","authors":"Y. Sanjaya, A. Fitriani, Annisa Martina Firdausa","doi":"10.57182/jbiopestic.15.1.26-30","DOIUrl":"https://doi.org/10.57182/jbiopestic.15.1.26-30","url":null,"abstract":"Spodoptera exigua (Hubner) is a polyphagous insect pest that is detrimental to farmers. Nanotechnology exists as a renewable technology that can increase effectiveness due to the small size of particles it produces. This study aimed to determine the effectiveness of biosynthesis of silver nanoparticles from the fungi Metarhizium, Trichoderma, and The Bacillus bacteria which are biocontrol agents. The research started in February and ended in May 2020, at the Biological Research Laboratory, Faculty of Mathematics and Natural Sciences Education, University of Pendidikan Indonesia. The experiment was based on a completely randomized design method (CRD). Onion armyworm (Spodoptera exigua), two isolates from the fungi Metarhizium and Trichoderma were obtained from the Indonesia Vegetable Research Institute (IVERRI). Each extract of Metarhizium and Trichoderma fungi and 50 mL of extract from Bacillus bacteria were added to 50 mL of Ag + 60 ppm solution in a 250 mL glass tube. The mixture of Metarhizium mushroom extract with 60 ppm Ag + solution or Trichoderma mushroom with Ag + 60 ppm solution was incubated at 400 C with a rotation speed of 200 rpm for five days under a dark condition. For the mixture of bacterial extracts with Ag + 60 ppm solution, incubation was at room temperature with a rotation speed of 150 rpm for three days. Analysis of the effect of NPP on the mortality of S. exiguawas done using the treatments, Metarhizum, Trichoderma, Bacillus, NPP-Metarhizium, NPP-Trichoderma, NPP-Bacillus, NPP, and distilled water as controls. Third instar larvae of S. exigua were immersed for 30 seconds. After immersion, the larvae are placed in sterile Petri dishes that have been lined with sterile filter paper and provided with caterpillar feed. Observation of caterpillar mortality was recorded at 2, 4, 6, 9, 24, 48, 64, 72, 96 and 108 hours after treatment. There were three replicates the results showed that all the treatments caused death of the insect, both entomopathogens and entomopathogenic nanoparticles. We found the three treatments of silver nanoparticles highly lethal to S. exigua. Two treatments, NPP-Metarhizium and NPP-Bacillus even caused 100% mortality after 120 hrs treatment. The possible reason for the pathogenicity of these products is considered to be biosynthesis of nanoparticles and enzymatic substances","PeriodicalId":15069,"journal":{"name":"journal of biopesticides","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"42965388","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"LABORATORY EVALUATION OF ESSENTIAL OIL CONSTITUENTS AGAINST THE TERMITES, ACISTROTERMES LATINOTUS","authors":"Azeez Om, Z. U, Mohammed G, Nwokerobi Ee","doi":"10.57182/jbiopestic.15.1.39-49","DOIUrl":"https://doi.org/10.57182/jbiopestic.15.1.39-49","url":null,"abstract":"In continuous search for alternative insecticides to combat potential problem of termite infestation in household and tree plantations; the bioactivity of the essential oils extracted by hydro distillation of leaves of Ocimum gratissimum, Gongronema latifolium and Piper guineensis was assessed under laboratory conditions at 20+30C, 70-75% R.H. and 12: 12h light: dark regimes for its biological activity against workers of the termites with Chlorpyrifos insecticide as a standard check. The botanicals were extracted using a Soxhlet extractor and serially diluted at different concentrations of 0.125%, 0.25% and 0.50% including 0% as the control. The essential oils at 0.25ml of the different concentrations of the botanicals and insecticide were applied using a syringe on twelve adult workers of termites in each Petri dishes. Mortality count or number of the termites knocked down after thirty, sixty, ninety and one hundred and twenty minutes respectively during the trial was recorded and expressed in percentages. The individual components of the essential oil were identified through GC, GC-MS and GC-Co injection with authentic standards. The identity of a total of 17 constituent compounds of the essential oil of each plant was confirmed and their relative proportion determined. The major compounds identified among the botanicals and the insecticide in their relative proportion included Alpha Pinene, Quinoline, 3- methyl and Benzylisoquinoline. Only Alpha Pinene was found in O. gratissimum and Chlorpyrifos insecticide, while Quinoline, 3 methyl and Benzylisoquinoline were recorded in the botanicals including the insecticide. The major components were found to be largely responsible for the toxic action of its essential oil against the termites. The highest dosage of the essential oils of the plant materials tested induced the highest mortality in the termites after 120 minutes. Within `120 minutes, O. gratissimum (55%) P. guineensis oil (52%) was significantly more effective than G. latifolium (47%) at the bioactive concentration of 0.25mg/L while Chlorpyrifos insecticide recorded the highest termite mortality. The termite mortality increased gradually with the increase in essential oil concentrations and with the time of exposure. There is need to diversify the use of botanicals especially in the agricultural pest management sector","PeriodicalId":15069,"journal":{"name":"journal of biopesticides","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"48865471","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"SUSTAINABLE CONTROL OF CODLING MOTH, CYDIA POMONELLA L. IN SOME APPLE ORCHARDS OF BULGARIA","authors":"Kutinkova H, D. V., Palagacheva N, S. I., St. Gandev G Kornov, B. Lingren","doi":"10.57182/jbiopestic.15.1.15-19","DOIUrl":"https://doi.org/10.57182/jbiopestic.15.1.15-19","url":null,"abstract":"The codling moth (CM), Cydia pomonella L., is the key pest of pome fruits in Bulgaria. Reduction of pesticide use is an important consideration for human health as well as for conservation of biodiversity. Environmentally friendly methods such as semiochemicals are among the most promising. These trials were carried out during the years 2020 and 2021 in southeast Bulgaria to test the effectiveness of microencapsulated sprayable pheromones in apple orchards using CIDETRAK® CM MEC (pheromone) liquid formulations as well as the effectiveness of mating disruption (MD) using CIDETRAK® CMDA COMBO™ MESO™ -A dispensers at rate of 80 dispensers per ha. Monitoring of CM flights was implemented using pheromone or pheromone and kairomone lures with traps during the years of the study. The traps baited with standard PHEROCON® CM L2 lures were changed at 4-week intervals. We also used PHEROCON® CMDA COMBO™ - P + AA lures, which is a new product developed by Trécé Inc., USA for use in orchards with MD. The traps baited with PHEROCON® CM DA COMBO - P + AA lures were changed at 8-week intervals. The damage in the treatment plot increased slowly over time and even in late cultivars, fruit damage by CM was below the economical threshold from 0.1 to 0.2% in both years of the study. Comparatively, ten insecticide treatments were applied to the reference orchard located in the region during the season, targeting CM and other pests. Correspondingly, fruit damage at harvest in the reference orchard by CM was from 2.2 to 2.5%. The significance of differences in the damage rate between the trial and the reference orchard was estimated by Chi-square tests. These new products developed by Trécé Inc., USA can be used in organic farming and fits perfectly into any IPM system. The use of CIDETRAK® CM MEC and CIDETRAK® CMDA COMBO™ MESO™ - A dispensers will help growers to decrease the number of chemical treatments in the field. Introduction of these products for pest management should result in reduction of the use of conventional chemical insecticide treatments, thus resulting in less environmental pollution and improved food quality.","PeriodicalId":15069,"journal":{"name":"journal of biopesticides","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"44023526","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"EVALUATION OF TEA TREE EXTRACT FORMULATION FOR THE CONTROL OF THE COTTON APHID, APHIS GOSSYPII (HOMOPTERA: APHIDIDAE) ON CAPSICUM ANNUUM IN THE GLASSHOUSE","authors":"Q. Ahmed","doi":"10.57182/jbiopestic.15.1.31-38","DOIUrl":"https://doi.org/10.57182/jbiopestic.15.1.31-38","url":null,"abstract":"Under laboratory and glasshouse conditions, the insecticidal activities of the essential oil obtained from leaves of the tea tree plant by steam distillation was evaluated against the cotton aphid, Aphis gossypii. The results showed that tea tree essential oil affected the aphid population causing a higher mortality of A. gossypii. This study showed that the 50 μL/mL concentration of tea essential oil caused 85.67% mortality after 24 hrs exposure time when used as contact treatment. However, the mortalities at 10, 20 and 30 μL/mL were 18.33, 21.67 and 70%, respectively, after 24 hrs of exposure. Conversely, the residual treatment was the most effective on the cotton aphid, with 100% mortality at the concentration of 50 μL/mL after 24 hrs of exposure to the essential oil. In contrast, the mortalities of A. gossypii were 15.00, 24.44 and 92.78% at the concentrations of 10, 20 and 30 μL/mL, respectively, after 24 hrs of exposure. The formulation of the tea tree essential oil was enormously potent in reducing the cotton aphid population on the sweet pepper, but was slightly phytotoxic to the potted plants. No significant differences were observed between tea tree oil formulations compared with the chemical pesticides, Karate and botanical insecticide, Levo. Based on our findings, the tea tree essential oil can be used as a botanical pesticide against A. gossypii after modifying it to reduce the phytotoxicity on the plants.","PeriodicalId":15069,"journal":{"name":"journal of biopesticides","volume":"94 2","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41297405","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"INSECTICIDAL POTENTIAL OF CERTAIN COMMON FERNS ON RUGOSE SPIRALLING WHITEFLY, ALEURODICUS RUGIOPERCULATUS MARTIN (HEMIPTERA: ALEYRODIDAE)","authors":"S. D., S. S., Rehanabanu H, SanthanaBharathi N","doi":"10.57182/jbiopestic.15.1.50-58","DOIUrl":"https://doi.org/10.57182/jbiopestic.15.1.50-58","url":null,"abstract":"There is a search for plant based alternatives to synthetic chemicals to control pests in crop plants were started from any years back. In this study, the efficacy of common ferns to control the whitefly, Aleurodicus rugioperculatus Martin (Hemiptera: Aleyrodidae) in coconut plantation was investigated. The whiteflies are prevalent throughout the year in the coconut fields and cause severe losses to the farmers. Ferns such as Adiantum caudatum, Hemionitis opposita, Pteris biaurita, Thelypteris meeboldii and Nephrolepis cordifoliawere evaluated for their efficacy in the control of the whitefly, A. rugioperculatus. The aqueous extracts of the ferns were screened at different concentrations viz., 1.0, 2.0, 3.0, 4.0 and 5.0% against A. rugioperculatus under laboratory conditions. Extracts from H. opposita and Thelypteris meeboldii showed the maximum mortality rate of 87% followed by Nephrolepis cordifolia (86%), Adiantum caudatum (83%) and Pteris biaurita (77%) at 5% concentration after 96 hours of treatment. The phytochemical screening of the extracts showed a qualitatively increased level of saponins in H. opposita and T. meeboldii. Reduced chlorophyll, carbohydrate and amino acid content were observed in the whitefly's infested leaves and increased protein content was observed in the infested leaves. Based on the study, the aqueous extracts of H. opposita and T. meeboldii can be recommended for the whitefly control in coconut plantations","PeriodicalId":15069,"journal":{"name":"journal of biopesticides","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"49157504","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"EVALUATING REPRODUCTIVE ABILITY OF THE RICE WEEVIL (PICIA MESOPOTAMICA) AT DIFFERENT TEMPERATURES ON RICE","authors":"M. M., Khudheyer A Alnomani, Abdulla Nezar Ali","doi":"10.57182/jbiopestic.15.1.09-14","DOIUrl":"https://doi.org/10.57182/jbiopestic.15.1.09-14","url":null,"abstract":"Rice (Oryza sativa L.) is one of the most important grain crops in Iraq. This crop is plagued by several significant pests, including the rice weevil aquatic (Picia mesopotamica) which is a serious pest in rice fields. This study was conducted out to learn more about the biological and environmental factors that influence how this insect survives and poses a threat to rice crops in the field. The results of reproductive ability revealed that temperature seems to have a significant impact on females' life cycles and survival rates during the first reproduction. Female egg productivity rates were found to be regulated by temperature, with the lowest rate occurring later in the female's life cycle. Moreover, the average age at the first reproduction of the female was 21 and 10 days at temperature 30°C and the highest rate of 30 and 19 days at temperature 20°C respectively and the highest average number of eggs 294.20 eggs/female at the temperature was 30°C. Further, the lowest average age of the female at first reproduction was 10 days at 30°C and the highest was 19 days at 20°C. The results also indicated the apparent effect of the tested temperatures on the net compensation rates of the insect females with the lowest being 8.793 females/generation at 35°C with the highest112.68 females/generation at30°C and the lowest average generation length 57.68 days at temperature. The population grew at an exponential rate of 0.035 females per day at 30°C, while the population grew at the slowest pace of 0.008 females per day at 35°C. Despite of the effect of temperature on other biological aspects of rice weevils requires more research. We found that temperature has a clear effect on the rice weevils reproductive activities on rice plants, and this research could be crucial in developing a successful pest management method.","PeriodicalId":15069,"journal":{"name":"journal of biopesticides","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"48897496","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"RELATIVE EFFICACY OF SOME BIORATIONAL AND MICROBIAL INSECTICIDES AGAINST YELLOW STEM BORER AND WHORL MAGGOT OF BORO PADDY","authors":"","doi":"10.57182/jbiopestic.14.2.90-96","DOIUrl":"https://doi.org/10.57182/jbiopestic.14.2.90-96","url":null,"abstract":"A field trial was conducted at Rice Research Station, Hooghly of West Bengal during boro season to evaluate the effect of some biorational and microbial insecticides against rice insect-pests. Insecticides of different origins such as, Beauveria bassiana Balsamo-Crivelli, Bacillus thuringiensis Berliner, Metarhizium anisopliae Metchnikoff, spinosad, azadirechtin, and cartap hydrochloride as check, have been tested in different combinations against yellow stem borer (Scirpophaga incertulas Walker) and whorl maggot (Hydrellia spp. Robineau-Desvoidy). The lowest whorl maggot infestation was recorded in spinosad treated plots (1.84%) followed by B. thuringiensis var. kurstaki (2.58%) and azadirachtin treatment (2.59%). The lowest yellow stem borer infestation as dead heart was recorded in plots treated with spinosad (2.92%) followed by M. anisopliae (3.32%) followed by azadirachtin (3.44%). The white ear head damage was recorded lowest in spinosad (8.04%) followed by B. bassiana + B. thuringiensis var. kurstaki (9.25%) and M. anisopliae treatment (9.48%). The highest grain yield and straw yield of boro rice were obtained from the plots treated with spinosad (57.72 q ha-1 grain yield and 6958 kg ha-1 straw yield) followed by B. bassiana + B. thuringiensis var. kurstaki (54.31 q ha-1 grain yield and 6681 kg ha-1 straw yield) followed by B. bassiana + M. anisopliae (53.47 q ha-1 grain yield and 64.58 q ha-1 straw yield). The overall results revealed spinosad as the most effective insecticide against both the insect species whorl maggot and yellow stem borer, showing minimum white ear head and dead heart with higher grain and straw yield.","PeriodicalId":15069,"journal":{"name":"journal of biopesticides","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"44123624","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"EVALUATION OF SOME DICOTYLEDONOUS SEED EXTRACTS AGAINST COCONUT LEAF BEETLE, BRONTISPA LONGISSIMA (GESTRO) (COLEOPTERA: CHRYSOMELIDAE) AND SUBLETHAL EFFECT TO AN ENDOPARASITOID","authors":"Bryan V. Novio, M. Zipagan, A. K. S. Amarga","doi":"10.57182/jbiopestic.14.1.01-11","DOIUrl":"https://doi.org/10.57182/jbiopestic.14.1.01-11","url":null,"abstract":"Aqueous and ethanolic seed extracts of Annona squamosa L. and Mangifera altissima B. were evaluated in the laboratory on adult coconut leaf beetles, Brontispa longissima G. Direct-spray method was employed in delivering the treatments to the test beetles. On the other hand, dip method was used in exposing parasitized pupae to different treatments. Laboratory results revealed that aqueous and ethanolic extracts of M. altissima at 15% w/v and 10% w/v, respectively and A. squamosa at 2%w/v and 10%w/v, correspondingly were comparable to Thiametoxam™. Percentage mortalities observed for all seed extracts used except the aqueous form of M. altissima exhibited a dose-dependent manner. Probit analysis revealed that the trend for LC50 for each seed extracts is M. altissima aqueous extracts (MAAE) >A. squamosa aqueous extracts(ASAE) >M. altissima ethanolic extracts (MAEE) >A. squamosa ethanolic extracts (ASEE). It was also found that based on recorded mean parasitoid emergence, Thiametoxam™ had greater sub-lethal effect than the botanicals on the natural enemy of coconut leaf beetle, Tetrastichus brontispae F. (5.00 ± 3.00). The chemical groups such as phenols, saponins and tannins might be possibly responsible for the observed bioactivities against the hispid beetle. UV-vis analyses of ethanolic fractions further revealed presence of secondary metabolite known to have several biological activities; annonasquacin for A. squamosa and mangiferin for M. altissima. Proper formulation of these materials can be developed as alternative control to B. longissima as they may offer selectivity than conventional insecticide and contain mixture of phytochemicals that can deter development of insect resistance.","PeriodicalId":15069,"journal":{"name":"journal of biopesticides","volume":"1 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41823589","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"ENTOMOTOXICANT POTENTIAL OF ARAK SHOOT EXTRACT FOR THE CONTROL OF EPHESTIA CAUTELLA","authors":"L. A. Al-Keridis","doi":"10.57182/jbiopestic.13.2.110-118","DOIUrl":"https://doi.org/10.57182/jbiopestic.13.2.110-118","url":null,"abstract":"The date, palm, Phoenix dactylifera is the most significant agricultural product in the Middle East region, in which Saudi Arabia is ranked as the top producing country. One of the most critical problems facing date production is insect infestation. Ephestia cautella is one of the most harmful insects to date, causing significant damage. Different techniques have been used against E. cautella through the use of synthetic chemical pesticides, but they have a negative impact on the environment. Recently, research has been directed towards seeking alternatives to these methods.In the current study, a biological system using Salvadora persica shoot extract for the control of E.cautellahas been evaluated. To this end, mortality rates LD50 and LD90 were determined when the different concentrations of the plant extract were applied at different developmental stages (larvae, pupa, and adult male/female) over a period of three days. Furthermore, some chemical constituents of the plant extract were determined by preliminary chemical tests and by using Gas chromatography–mass spectrometry (GC-MS). Results from current investigation have displayed that the mortality rate was dependent on both the developmental stage and concentrations of the plant extract. Total phenolics and the saponin content of the plant extract were detected. GC-MS analysis identified the potential chemical agents in the plant extract that may possess the insecticidal ability. In light of the current findings, it is clear that infestation of date palms by E.cautella can be controlled by using S. persica shoot extract. To optimize the implementation of this technique, we recommend that future research focus on testing other organic solvents and other plant parts so that the most biologically active biomolecules responsible for the control of E. cautella under date storage conditions can be accurately determined.","PeriodicalId":15069,"journal":{"name":"journal of biopesticides","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2020-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"45176769","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"CHEMICAL STUDY AND EVALUATION OF INSECTICAL PROPERTIES OF AFRICAN LIPPIA CITRIODORA ESSENTIAL OIL","authors":"F. Abdoul-Latif, A. Ainane, T. M. Abdoul-Latif, Tarik Ainane","doi":"10.57182/jbiopestic.13.2.119-126","DOIUrl":"https://doi.org/10.57182/jbiopestic.13.2.119-126","url":null,"abstract":"The main objective of this study is to highlight the chemical composition, the insecticidal and the antibacterial activities of essential oil of Lippia citriodora growing spontaneously in central Morocco, particularly in Beni Mellal. After extracting the essential oil by a clevenger type distiller from the aerial parts, all constituents were analyzed by gas chromatography coupled with mass spectrometry (GC/MS). Their chemical composition of chemotype revealed the presence of 45 compounds in the essential oil, which has Geranial as the major constituent with a content of approximately (35.45%), followed by Neral (26.11%) and Limonene (14.06%), with a total percentage of (75.62%). The insecticidal activities were carried out against three weevils:Sitophilus granarius, Sitophilus oryzae and Sitophilus zeamais, the results of this test to show a very important activity during 24 hours of the treatments confirmed by the values of LD50 and LD90 which tend towards 0, or this activity was probably due to the major constituents. The bacterial power was studied in vitro against gram-positive bacteria: Bacillus cereus (CECT 193) and Listeria monocytogenes (CECT 4032), gram-negative bacteria: Yersinia enterocolitica (CECT 9144), Pseudomonas aeruginosa (CECT 116) and Staphylococcus aureus (CECT 239), the results of this tests made it possible to quantitatively assess the bacterial power by looking for minimum MIC inhibitory concentrations and minimum MBC bactericidal concentrations, which have a varying activity depending on the strain tested.","PeriodicalId":15069,"journal":{"name":"journal of biopesticides","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2020-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"45408000","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}