journal of biopesticides最新文献

{"title":"BIOLOGICAL CONTROL OF CODLING MOTH, CYDIA POMONELLA (L.) IN APPLE ORCHARDS OF BULGARIA USING GINKO® DISPENSERS","authors":"H. Kutinkova, V. Dzhuvinov, N. Palagacheva, I. Staneva, S. Gandev, G. Kornov, M. Tityanov","doi":"10.57182/jbiopestic.15.2.103-109","DOIUrl":"https://doi.org/10.57182/jbiopestic.15.2.103-109","url":null,"abstract":"The codling moth, Cydia pomonella (L.) (Lepidoptera: Tortricidae), is a significant pest of pome fruit and walnut orchards worldwide and it is a particular problem in Bulgaria. Codling moth resistance to many insecticides has been recently detected in Bulgaria. Codling moth (CM) mating disruption with Ginko® dispensers was evaluated in four successive years from 2019 to 2022 as part of an anti-resistance strategy. In an isolated 3 ha apple orchard in the Plovdiv district of South-Central Bulgaria, close to the town of Perushtitsa, field tests were conducted for the control of the codling moth (CM), C. pomonella, via mating disruption (MD) using pheromone Ginko® dispensers. The pheromone dispensers were hung in the upper third of tree canopies with a density of 500 dispensers/ha before CM flight started. Dynamics of CM flights was monitored by pheromone traps installed in the trial plot and in a conventionally treated reference orchard. Two types of baits were used in the traps: PHEROCON® CM L2–codlemone lures and PHEROCON® CM DA COMBO-P + AA lures (Trécé Inc., USA) in the orchards for mating disruption. The PHEROCON® CM L2 lures were changed at 4-week intervals and PHEROCON® CM DA COMBO-P + AA lures at 8-week intervals. PHEROCON® VI Delta sticky traps baited with PHEROCON CM DA COMBO- P + AA lures and standard CM L2 caps were installed, for comparison, in a reference orchard located in the Plovdiv region. Fruit infestation was periodically assessed till the harvest time. In both years fruit damage remained lower till late July and increased slightly only in August. At harvest, fruit damage remained below 1%. It was obvious that mating disruption could be useful strategy for the control of codling moth in Bulgarian apple orchards, specifically when the plot is isolated from external sources of infestation and the population density of the pest is low.","PeriodicalId":15069,"journal":{"name":"journal of biopesticides","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"43277561","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"INSECTICIDAL PROPERTY OF TERPENES AGAINST MAIZE WEEVIL, SITOPHILUS ZEAMAIS (MOTSCHULSKY)","authors":"M. Chaubey","doi":"10.57182/jbiopestic.15.2.91-102","DOIUrl":"https://doi.org/10.57182/jbiopestic.15.2.91-102","url":null,"abstract":"Synthetic pesticides are used indiscriminately in insect pest management, which damages the ozone layer and causes resistance in target organisms as well as neurotoxicity, carcinogenicity, teratogenicity and mutagenesis in non-target organisms. Due to these negative consequences, research is now focused on using plant-based techniques to control insect pests. The maize weevil, Sitophilus zeamais (Coleoptera: Curculionidae), was tested in the laboratory to determine the insecticidal effects of two pure essential oil constituents, namely α-pinene and β-caryophyllene. These two terpenes were tested against S. zeamais for their toxic, ovipositional, developmental, and feeding inhibitory effects. When S. zeamais adults were fumigated for 24 and 48hrs, the median lethal concentrations (LC50) of α-pinene and β-caryophyllene were 0.412 and 0.305 μlcm-3 and 0.486 and 0.315 μlcm-3 air respectively. When S. zeamais adults were exposed for 24 and 48hrs in a contact toxicity assay, the LC50 values for α-pinene and β-caryophyllene were 0.388 and 0.256 μlcm-2 and 0.308 and 0.216 μlcm-2 area respectively. Adults exposed to sub-lethal concentrations of both terpenes experienced decreased acetylcholine esterase (AChE) enzyme activity. In S. zeamais, α-pinene and β-caryophyllene decreased oviposition, progeny output and eating. According to this study, α-pinene and β-caryophyllene can be used to make environmentally acceptable formulations and as a substitute for synthetic insecticides.","PeriodicalId":15069,"journal":{"name":"journal of biopesticides","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"49222220","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"BIOLOGICAL ACTIVITY OF CITRULLUS COLOCYNTHIS L EXTRACTS ON CULEX PIPIENS MOLETHUS AND MUSCA DOMESTICA","authors":"Khudheyer A. A. Alnomani, Abdulla N. Ali, M. Mubasher","doi":"10.57182/jbiopestic.15.2.150-161","DOIUrl":"https://doi.org/10.57182/jbiopestic.15.2.150-161","url":null,"abstract":"The two most hazardous vectors for humans are Culex pipiens molethus and Musca domestica. Several environmental and health issues have emerged because of the overuse of chemical pesticides. This study sought to determine the efficacy of aqueous, alcohol, and oil extracts of Citrullus colocynthis L. plant under in vitro conditions using different concentrations (0, 5, 10, and 20 mg/mL) on the various stages of Culex pipiens molethus and Musca domestica. It also sought to identify natural biological alternatives to synthetic insecticides. The current research revealed that the adult and juvenile stages of mosquitoes and house flies were considerably impacted by the extracts of bitter melon. Concentrations demonstrated a distinct effect and significantly outperformed the control treatment after 24, 48, and 72 hrs of exposure. Compared to other bitter melon extracts, the impact of the oil extract of C. colocynthis was superior. At a dosage of 20 mg/ml, oil extract treatment significantly reduced hatchability and increased mortality in the second and fourth larval stages of C. pipiens molethus. The outcomes also demonstrated the superiority of the oil extract of C. colocynthis in influencing the different stages of the house fly M. domestica with the same previous concentrations (20, 10,5 mg/mL) on egg productivity (22, 72, 89, 82%) and third larval instar (66, 79, 71, 94, 82.88%) mortality rates, as well as pupae weight rates (0.10, 0.11, 0.100 mg). With the help of the current study's findings, we may suggest using bitter melon extracts, particularly the oil extract, as a potentially effective control measure against mosquitoes and house flies. These naturally occurring extracts, which do not contain any industrial chemicals, are difficult for the insect they are intended to kill to withstand. For the aim of understanding the mechanism of action of these extracts on mosquitoes and house flies, as well as understanding the effect of bitter melon extracts on numerous therapeutic insects in the future, more research is necessary.","PeriodicalId":15069,"journal":{"name":"journal of biopesticides","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"42629803","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"PLANT STEM INFUSED WATER ON MOSQUITOCIDAL ACTIVITY TO CULEX QUINQUEFASCIATUS SAY","authors":"K. Sahayaraj, S. Iyappan, Manickam Jothimuthu, C. Ajitha, I. Juliet, G. Petchidurai","doi":"10.57182/jbiopestic.15.2.110-117","DOIUrl":"https://doi.org/10.57182/jbiopestic.15.2.110-117","url":null,"abstract":"Locally available plants, Azadirachta indica, Citrus aurantifolia, Citrus aurantium and Tamarindus indica stems were infused in water and tested efficiency against Culex quinquefasciatus Say (Diptera: Culicidae) life stages. Preliminary phytochemical profiling of the plant infused water reveals the presence of phenolic compounds, tannins, flavonoid, cardiac glycosides and terpenoids. Tannins quantification revealed that C. aurantifolia had significantly more amounts than other plants. Citrus aurantium stem infused water (CASIW) caused more mortality to C. quinquefasciatus larvae and pupae. Furthermore, CASIW increased larval and pupal developmental period and reduced adult longevity. However, tested plants did not affect the morphology of the insect. Results suggested utilize this indigenous technique to minimize C. quinquefasciatus population.","PeriodicalId":15069,"journal":{"name":"journal of biopesticides","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41954500","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"EVALUATION OF NATIVE ANTAGONISTS FOR THE MANAGEMENT OF STEM ROTS OF CLUSTER BEAN CAUSED BY SCLEROTIUM ROLFSII SACC.","authors":"A. Muthukumar, S. Raj, A. Sudha, R. Udayakumar","doi":"10.57182/jbiopestic.15.2.162-172","DOIUrl":"https://doi.org/10.57182/jbiopestic.15.2.162-172","url":null,"abstract":"Cluster bean, Cyamopsis tetragonoloba (L.) Taub is an important annual legume crop of kharif season in arid and semi-arid regions of the Indian subcontinent. Six fungal antagonists and four bacterial antagonists were evaluated against Sclerotium rolfsii in vitro and in vivo condition to develop an effective management strategy for stem rot of cluster bean. During in vitro studies, native fungal and bacterial antagonists were inhibited the mycelial growth of pathogen. Among the fungal antagonists tested, the isolate-I4 (THM) showed the maximum growth inhibition of S. rolfsii up to 68.10 per cent. The bacterial antagonist Isolate-I3 (PFV) recorded the maximum inhibition zone of 13.33 mm and a minimum of 23.00 mm mycelial growth of S. rolfsii. Pot culture studies revealed that the combined application of T9-T. harzianum (THM) + P. fluorescens (PFV) (ST+ SA) through seed treatment and soil application recorded the minimum incidence of stem rot (4.66 per cent) and maximum plant height, number of pods/plant and pod length.The findings reported in the present study supported the applicability of isolate-I4 (THM) Isolate-I3 (PFV) as a possible alternative to fungicides for the control of root rot in black gram.","PeriodicalId":15069,"journal":{"name":"journal of biopesticides","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"45918352","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"BIOFUMIGATION: PROSPECTS FOR CONTROL OF SOIL BORNE PLANT DISEASES","authors":"Manoj Sihag, Vipul Kumar, M. Rana, Seweta Srivastava, Shivam Singh, Divakar","doi":"10.57182/jbiopestic.15.2.136-149","DOIUrl":"https://doi.org/10.57182/jbiopestic.15.2.136-149","url":null,"abstract":"One of the biggest worries for farmers is the spread of pathogens through the soil. These diseases are difficult to control because they are often tiny in size, buried in the soil, and frequently highly harmful even in small numbers. The Montreal Protocol on Substances that Deplete the Ozone Layer, to which the majority of countries are signatories, has restricted the use of residual pesticides for the management of soil-borne infections, and the demand for food that is free of blemishes is rising. However, it has become urgently necessary to find suitable substitutes as a result of the phase-out of methyl bromide, a significant chemical. After introducing plants that contain glucosinolate, which is digested to produce isothiocyanates (ITC) in the soil, biofumigation has emerged as a crucial procedure to control plant diseases. The existence of glucosinolates and the byproducts of their hydrolysis in soil illustrate the effectiveness and environmental impact of biofumigation. The most significant producers of bioactive chemicals are Brassica species, which makes them suitable for biofumigation applications. This review focuses on the concept, the effective application of biofumigants against soil-borne diseases, and offers several case examples to highlight upcoming difficulties for the concept's continued advancement.","PeriodicalId":15069,"journal":{"name":"journal of biopesticides","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"47840665","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Efficacies of botanicals in the management of stem end rot disease of mango fruits","authors":"P. Tripathi","doi":"10.57182/jbiopestic.15.1.01-08","DOIUrl":"https://doi.org/10.57182/jbiopestic.15.1.01-08","url":null,"abstract":"The phytopathogenic fungus Botryodiplodia theobromae Pat., causes stem end rot disease of mango fruits. Extracts from twenty angiospermic taxa were evaluated for their antifungal activity. The extracts were prepared in water, acetone, ethyl alcohol, petroleum ether, ethyl acetate and methanol. Among the selected plants, essential oil (EO) of Adenocalymma alliaceum was found to be effective in controlling the growth of Botryodiplodia theobromae. The minimum inhibitory concentration (MIC) of the EO was 100ppm. The oil was found to withstand a high inoculum density. Plant extracts of Allamanda cathartica, Lawsonia inermis, Prunus persica and Adenocalymma alliaceum in different solvents showed inhibitory effect on B. theobromae. However, Leaf extract of Adenocalymma alliaceum in all the solvents namely, water, acetone, ethyl alcohol, petroleum ether, ethyl acetate and methanol was found to have antifungal activity. An enhancement in the shelf life of mango fruits was recorded under in vivo trial after treatment with aqueous extract and essential oil of A. alliaceum. It is concluded that the aqueous extract and EO of A. alliaceum has a great potential in the management of B. theobromae damage to mango fruits.","PeriodicalId":15069,"journal":{"name":"journal of biopesticides","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"46846331","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"IN VITRO ANTAGONISTIC ACTIVITY OF TRICHODERMA VIRIDE ISOLATES AGAINST SCLEROTINIA SCLEROTIORUM AND THEIR ROLE IN GROWTH PROMOTION OF COMMON BEAN","authors":"Nayeema Jan, Wasim Sajad Malik, Abdul Hamid Wani, Mansoor Ahmad Malik, Shaiesta Hassan, Mohd Yaqub Bhat","doi":"10.57182/jbiopestic.15.1.20-25","DOIUrl":"https://doi.org/10.57182/jbiopestic.15.1.20-25","url":null,"abstract":"A study was done to investigate the antagonistic effects of three Trichoderma viride isolates (TV1, TV2 and TV3) on Sclerotinia sclerotiorum. The results revealed that the isolate TV1 proved to be the best in suppressing the mycelial growth by 77% after 7 days of incubation. However 74.91% and 50% inhibition were recorded on 5th and 3rd days of incubation, respectively. The isolate TV2 caused inhibition of 74.77% on 7th day, and 72.66% and 48.33% after 5th and 3rd days of incubation, respectively. 72.54%, 70.41%, and 51.66% inhibition in mycelial growth of the pathogenic fungus was caused by TV3 isolate after 7th, 5th, and 3rd days of incubation respectively. All the test isolates of T. viride showed a significant effect on the vigour index of common bean. The maximum germination percentage was observed on treatment with TV1 isolate (85%) followed by TV2 (83%) and TV3 (81%) respectively, in comparison to untreated seeds which showed a germination percentage of 73%. Maximum vigour index was shown by those plants whose seeds were treated with TV1 (11560), followed by those treated with isolate TV2 (10707) and TV3 (9234), whereas the control plants showed significantly lower vigour index of 7446 in comparison to treated plants.","PeriodicalId":15069,"journal":{"name":"journal of biopesticides","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"48949297","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"PHYTOCHEMICAL SCREENING AND PESTICIDAL EFFICACY OF PHYTOECDYSTEROID FRACTION OF A FERN PITYROGRAMMA CALOMELANOS (L.) LINK AGAINST SPODOPTERA LITURA (FABRICIUS.)","authors":"Princy Rathnamala Jayaseeli J, S. P, Sherlin John J, Velankanny M","doi":"10.57182/jbiopestic.15.1.73-81","DOIUrl":"https://doi.org/10.57182/jbiopestic.15.1.73-81","url":null,"abstract":"Lepidopteran insect pests voraciously feed the vegetable crops and causes yield loss every year. Chemical insecticides widely used to control the destructive pest incidence which heavily affects the beneficial and non-target organisms and in the mean time, pests developed resistance. Biopesticides are being developed to minimize these ill effects. Plants synthesize secondary metabolites to prevent from biotic and abiotic stresses. More than 27 families of pteridophyta (ferns) are reported with phytoecdysteroids (PEs). PEs are analogues of insect moulting hormone and acts as insect growth regulators (IGRs). The present study aimed to isolate the phytoecdysteroid fraction from the fern, Pityrogramma calomelanos, preliminary screening of phytochemicals using standard protocols including UV-Visible spectrophotometric analysis and survival, growth and developmental periods of Spodoptera litura treated with phytoecdysteroid fraction. Steroids and phenolic compounds were present in the fraction and six prominent peaks were recorded between the wavelength of 200-800nm. Spodoptera litura larvae treated with phytoecdysteroid fraction showed maximum larval mortality (68%) in 2000ppm (LC50 = 1473ppm; F = 48.65; p = 0.049). Pupation, pupal weight and adult emergence were decreased in higher concentrations. Pupal mortality was relatively high in treated group than control (2.04%). Larvae treated with minimal concentrations exhibited developmental deformities includes larval - pupal, pupal -adult intermediates, deformed pupae, ecdysial failure, adultoids, early/late developmental periods and it may due to the interaction of phytoecdysteroid fraction to the insect endocrine system. Therefore, phytoecdysteroid mediated biopesticide formulation could be the better alternative to commercial chemical insecticides under IPM programme.","PeriodicalId":15069,"journal":{"name":"journal of biopesticides","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"43357241","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"BIOEFFICACY OF ENTOMOPATHOGENIC FUNGI METARHIZIUM ANISOPLIAE (METSCHN.) SOROKIN AGAINST THE COTTON STAINER, DYSDERCUS CINGULATUS (FAB.) (HEMIPTERA: PYRRHOCORIDAE)","authors":"Velankanny M, S. P, Francis Borgio J, Sherlin John J, Princy Rathnamala Jayaseeli J","doi":"10.57182/jbiopestic.15.1.59-72","DOIUrl":"https://doi.org/10.57182/jbiopestic.15.1.59-72","url":null,"abstract":"Entomopathogenic fungi play an important role in the biological control of the insect pest population in an agroecosystem. Metarhizium anisopliae is one such entomopathogenic fungus, proved to be an effective biocontrol agent against different insect pests. However, different strains of entomopathogens show variations in their pathogenicity and host specificity. Hence the present study is aimed to determine the bioefficacy of local isolates of the entomopathogenic fungus M. anisopliae to control Dysdercus cingulatus. Fungal strains were isolated from cotton fields in Tirunelveli, Thoothukudi, Thenkasi, and Viruthunagar districts of Tamil Nadu following standard protocols. Four different isolates were identified and used for the bioassay. Bioefficacy trials were carried out in all the five nymphal instars and the adults of D. cingulatus and were treated with eight different concentrations of M. anisopliae (101,102,103,104,105,106,107and 108 spores/mL). The formulations were evaluated for their pathogenicity and efficiency against D. cingulatusnymphal instars and adults which resulted in 70% to 100% mortality. A 100% mortality rate was observed in four isolates of M. anisopliae at higher concentrations (120 hrs) after treatment. Lethal concentration (LC50) values of M. anisopliae isolates against D. cingulatus were calculated as 5.94×107(ERUM1), 6.09×107(ERUM2), 2.62×107(ERUM3), 2.69×107(ERUM4). Approaching biocontrol agents instead of chemical pesticides seems to be very promising in the march towards more sustainable, eco-friendly agricultural pest management practices and protecting the environment","PeriodicalId":15069,"journal":{"name":"journal of biopesticides","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41631574","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}