Rohit S. Parchande, Anuradha K Shinde, S. Yankanchi
{"title":"CHEMICAL COMPOSITION AND INSECTICIDAL EFFICACY OF VITEX NEGUNDO L. ESSENTIAL OIL AGAINST FALL ARMYWORM, SPODOPTERA FRUGIPERDA (J. E. SMITH)","authors":"Rohit S. Parchande, Anuradha K Shinde, S. Yankanchi","doi":"10.57182/jbiopestic.16.1.16-23","DOIUrl":"https://doi.org/10.57182/jbiopestic.16.1.16-23","url":null,"abstract":"The fall armyworm, Spodoptera frugiperda, is a key pest ofmaize crops in tropical and subtropical regions and has developed resistanceagainst synthetic pesticides and Bacillus thuringiensis toxins. Thus,the experiments were designed to evaluate the chemical analysis andinsecticidal efficacy of Vitex negundo against S. frugiperda.Chemical analysis of V. negundo essential oil revealed a total of 36compounds, and the oil contained more monoterpenes (68.16%) than sesquiterpenes(22.21%) and diterpenes (13.56%). For the ovicidal and insecticidal activities,the essential oil was found to be most effective, with a median lethalconcentration (LC50) value of 51.31 µL/mL and 0.29 L/larvae, respectively.Larval antifeedant and growth inhibitory activity of essential oil werepositively correlated (R2 = 0.9645) and (R2 = 0.9727) with oil concentrations,respectively. Therefore, the V. negundo essential oil can be used forthe development of bioinsecticides to control S. frugiperda, a highlyinvasive and polyphagous pest, which will replace chemical pesticides.","PeriodicalId":15069,"journal":{"name":"journal of biopesticides","volume":"62 24","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141231389","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
R. N. Raman, K. V. Kumar, Prahlad Masurkar, Angel Jemima
{"title":"EVALUATION OF TRICHODERMA ASPERELLUM BIOFORTIFIED WITH VERMI COMPOST AGAINST FUSARIUM OXYSPORUM F.SP. LYCOPERSICI","authors":"R. N. Raman, K. V. Kumar, Prahlad Masurkar, Angel Jemima","doi":"10.57182/jbiopestic.16.1.24-32","DOIUrl":"https://doi.org/10.57182/jbiopestic.16.1.24-32","url":null,"abstract":"Morphological features are not adequate to accurately categorizedifferent species of the genus Trichoderma. Molecular characters, incombination with morphological characters, are used to identify Trichodermaat the species or subspecies level. The present study was focused oncharacterising Trichoderma asperellum based on morphology and molecularanalysis using genes such as ITS. Light microscopy results showed that Trichodermaasperellum regularly branched and typically paired conidiophores withstraight phialides and globose to subglobose shaped conidia Sequence similarityanalysis with reference T. asperellum isolates available in the NCBIdatabase showed 100 percent nucleotide similarity for ITS4 and ITS5. A dualculture test clearly showed that Trichoderma asperllum inhibited thetested fungal plant pathogen, Fusarium oxysporum f. sp. lycopersici(87.38 percent). Population dynamics of Trichoderma asperellum incompost were found to be stable until 21 days after mixing.","PeriodicalId":15069,"journal":{"name":"journal of biopesticides","volume":"3 11","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141229592","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"PHYLOGENETIC CONNECTIONS AND COMPARISONS OF THE AMINO ACID SEQUENCES OF SEVERAL PEPTIDE INDUCERS OF MICROBIAL ORIGIN- A FOUNDATION STUDY FOR DEVELOPMENT OF NOVEL BIOPESTICIDES","authors":"Suvala Shalini Devi, Chanduri Akshitha, Aparna Bandaram, Bomma Sharath Chandra, Vulishe Vaishnavi","doi":"10.57182/jbiopestic.16.1.09-15","DOIUrl":"https://doi.org/10.57182/jbiopestic.16.1.09-15","url":null,"abstract":"Peptides of microbial origin stand out as one of theprominent tools to elicit plant immunity. These peptides comprise one of thekey strategies of integrated pest management and are considered as candidatesto develop novel biopesticides. Manyresearch investigations have proved their potential in fending off plantpathogens and were described as sustainable plant protection strategies. Thepresent study was attempted to discover phylogenetic relationships and compareamino acid sequence alignments of various peptide elicitors of microbialorigin. Phylogenetic analysis of 33 microbial peptide elicitors resulted in twoclusters, one cluster contained 19 flagellin sequences, which is furtherdivided into one major (15 peptide sequences) and one (4 peptide sequences)minor subclusters. Further amino acid sequence alignments were carried outbased on the evolutionary relationships among the peptides. The amino acidsequence alignment of flagellin sequences using Clustal W did not present conservativeamino acid sequences except Serine (S), Alanine (A) and Aspartic acid (D).These conserved amino acids (SAxD) that are positioned in the protruding loopmay play a vital role in recognition by plant surface receptors. Alignment ofamino acid sequences of cold shock protein, xylanase, elongation factor andharpin from various sources did not present conservative amino acid sequencesexcept glycine. These investigations lay a theoretical foundation for exploringmany more microbial peptides for inducing plant resistance.","PeriodicalId":15069,"journal":{"name":"journal of biopesticides","volume":"45 22","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141232527","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
M. Velankanny, Parthas Selvaraj, T. Pushpanathan, B. Ravichandran, J. Borgio, J. P. R. Jayaseeli, J. John
{"title":"PATHOGENICITY OF NATIVE BEAUVERIA BASSIANA (BALSAMO-CRIVELLI) VUILLEMIN ISOLATE ON DYSDERCUS CINGULATUS (HEMIPTERA: PYRRHOCORIDAE)","authors":"M. Velankanny, Parthas Selvaraj, T. Pushpanathan, B. Ravichandran, J. Borgio, J. P. R. Jayaseeli, J. John","doi":"10.57182/jbiopestic.16.1.38-44","DOIUrl":"https://doi.org/10.57182/jbiopestic.16.1.38-44","url":null,"abstract":"Entomopathogenic fungi (EPF) are the microorganisms thatspecifically infect and often kill insects and other arthropods. EPF is themost effective biocontrol agent against insects in the natural ecosystem whichcould be an effective alternative to chemical insecticides in bio-intensivepest management. Beauveria bassiana, one of the most prevalentsoil-borne entomopathogens, has virulence on insect pests. The present study isaimed to evaluate the pathogenicity of a native isolate of the entomopathogenicfungus B. bassiana isolated from the soil samples of a cotton field(Kuthukkal) in the Tirunelveli district of Tamil Nadu against Dysdercuscingulatus. Bio-efficacy trials were carried out with six differentconcentrations viz., 4.6 × 103,1.5× 104, 5.0 × 105, 2.7 × 106, 3.2 × 107,and 2.8 x 108 (spores/mL) in all the five nymphal instars and theadults of D. cingulatus. A 100% mortality was observed in higherconcentrations 2.8 x 108 (spores/mL) at 120hrs after treatment. The resultsof the present study show that the isolate seems to be highly promising in thepest management of D. cingulatus.","PeriodicalId":15069,"journal":{"name":"journal of biopesticides","volume":"74 6","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141234342","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Adlin Pricilla Vasanthi, S. J. Nelson, B. Sangeetha
{"title":"REPELLENT AND GROWTH REGULATORY EFFECTS OF LANTANA CAMARA EXTRACTS ON ODONTOTERMES WALLONENSIS (ISOPTERA: TERMITIDAE)","authors":"Adlin Pricilla Vasanthi, S. J. Nelson, B. Sangeetha","doi":"10.57182/jbiopestic.16.1.33-37","DOIUrl":"https://doi.org/10.57182/jbiopestic.16.1.33-37","url":null,"abstract":"Odontotermes wallonensis is a fungus growing termite species that are abundant inSouth India. Lantana camara leavesextracted with methanol have been found to have repellent and IGR activitiesagainst termites. The extract was also tested on O. wallonensis nymphs and showed significant IGR activity at aconcentration of 1 %.","PeriodicalId":15069,"journal":{"name":"journal of biopesticides","volume":"7 8","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141229215","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"ROLE OF PITYROGRAMMA CALOMELANOS (L.) LINK IN DIETARY AND NUTRITIONAL INDICES OF SPODOPTERA LITURA (F.) (LEPIDOPTERA: NOCTUIDAE)","authors":"J Princy Rathnamala Jayaseeli, Selvaraj P, Pushpanathan T, Anbu Radhika S, Sherlin John, Velankanny M","doi":"10.57182/jbiopestic.16.1.01-08","DOIUrl":"https://doi.org/10.57182/jbiopestic.16.1.01-08","url":null,"abstract":"The polyphagous, ubiquitous, destructive pest, Spodoptera litura(F.) affects 150 host plant species and notably their larval forms arenotorious. Extensive feeding and rapid reproduction cycles led the farmers andindustrialists to seek the effective chemical and synthetic pesticides. Randomand inappropriate usages of pesticides imposed a negative impact on non-targetorganisms, environment and abiotic factors and also pest resurgence to theparticular pesticides. To overcome this unnatural incidence, biopesticides arepracticed as an alternative source. Plant botanicals play a huge role as insectgrowth regulators (IGRs) by inhibiting the regular moulting cycle, developmentand survival of insect pests. Also, the silverback fern, Pityrogramma calomelanosare reported with several secondary metabolites which are known for theirpesticidal properties. Hence, the present work was framed to study the dietaryresponses as well as nutritional indices of S. litura treated with crudeextract of P. calomelanos. The treated larvae showed dietary andpost ingestion responses prior to moulting and death. On the 4th day oftreatment, reduction in food intake (522.89 mg), larval weight gain (41.02 mg),ECI (56.25 %), ECD (46.30 %), AD (47.21 %) and RGR (3.06 mg/mg/day) wasobserved as dose dependent manner except the feacal production (378.18 mg) ascompared to control. Therefore, the ethanolic extract of a fern, P. calomelanoscan be recommended as a biopesticides for its post ingestion andanti-nutritional effects on S. litura larvae.","PeriodicalId":15069,"journal":{"name":"journal of biopesticides","volume":"217 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-06-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"136366737","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Shaiesta Hassan, A. Wani, Nayeema Jan, M. Bhat, Waseema Jan, T. A. Wani
{"title":"BIO-MANAGEMENT OF FUNGAL LEAF SPOT OF TOMATO (SOLANUM LYCOPERSICUM L.) USING INDIGENOUS TRICHODERMA ISOLATES","authors":"Shaiesta Hassan, A. Wani, Nayeema Jan, M. Bhat, Waseema Jan, T. A. Wani","doi":"10.57182/jbiopestic.15.2.122-128","DOIUrl":"https://doi.org/10.57182/jbiopestic.15.2.122-128","url":null,"abstract":"One of the main obstacles to feeding the world is the fungal leaf spot disease, which has a negative impact on plants' photosynthetic areas and significantly lowers crop quality and output. To manage fungi that pose a serious hazard to both humans and the environment, various chemical fungicides are utilized. The goal of the current study was to determine whether local isolates of three Trichoderma spp. (Trichoderma viride, Trichoderma harzianum, and Trichoderma asperellum) had any antagonistic effects in vitro against the pathogenic fungi Alternaria alternata, Fusarium solani, Fusarium oxysporum, Aspergillus flavus, Aspergillus sydowii, and Alternaria sp. Trichoderma viride showed the greatest growth inhibition against all of the tested pathogenic fungi, followed by Trichoderma harzianum and Trichoderma asperellum. It was shown that all three Trichoderma species strongly inhibited the mycelial growth of fungal pathogens. Compared to other isolated fungi, Trichoderma species inhibited Alternaria alternata mycelial growth more, whereas in Fusarium oxysporum least amount of mycelial growth inhibition was observed. These findings imply that Trichoderma species can function as an effective biocontrol agent against the fungi responsible for tomato leaf spot disease.","PeriodicalId":15069,"journal":{"name":"journal of biopesticides","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"43486119","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
J. John, Prathas Selvaraj, T. Pushpanathan, B. Ravichandran, S. Abdulazeez, J. Borgio
{"title":"THE SEQUENCE BETWEEN MITOCHONDRIAL NAD1-NAD2 GENES ARE POTENTIAL MOLECULAR MARKERS OF HARPACTORINAE PREDATORS (HETEROPTERA: REDUVIIDAE)","authors":"J. John, Prathas Selvaraj, T. Pushpanathan, B. Ravichandran, S. Abdulazeez, J. Borgio","doi":"10.57182/jbiopestic.15.2.129-135","DOIUrl":"https://doi.org/10.57182/jbiopestic.15.2.129-135","url":null,"abstract":"Assassin bugs are terrestrial predators belonging to the family Reduviidae. Among the 24 subfamilies, harparctorinae is predominant and extensively investigated for biocontrol applications compared to the others subfamilies in Reduviidae. Being natural enemies of phytophagous insects, understanding the phylogeny of these predatory bugs can precise the selection of candidates to employ in insect pest management. In addition to morphological systematics, complete mitochondrial genome sequences provide great insights into the phylogeny for resolving evolutionary complexity. Complete mitochondrial genomes of four potential predatory harpactorinae and one outgroup triatominae were retrieved from NCBI GenBank database. Comparative analysis of the five mitogenomes and the nucleotide sequence between nad1 and nad2 genes were selected as the best option to distinguish. The nucleotide sequence between nad1- nad2 are found to be biased towards A and T similar to their respective complete mitogenomes. Tajima’s test of neutrality suggest that the evolutionary selection at nad1-nad2 was parallel to the complete mitogenome and showed positive and significant (p > 0.1) with high nucleotide diversity. Unequal evolutionary rate at nad1-nad2 between lineages observed in Tajima’s relative rate test and proved the nucleotide sequences of nad1-nad2 between species are highly variable. Comparing the phylogenetic trees generated using the complete mitogenomes and nad1-nad2 genes uncovered the correlation between the trees and having identical branches with varying bootstrap values. Conventionally the highly conserved protein-coding cox1 gene is used for molecular taxonomy whereas this study provides an additional and/or a possible alternative molecular marker for genetic comparative test (the nucleotide sequence between nad1-nad2) to understand the systematics and phylogeny of Reduviidae. The significant nucleotide diversity, high genetic distance and less genetic similarity of the sequence between nad1-nad2 genes among the species studied, Agriosphodrus dohrni, Rhynocoris fuscipes, Scipinia horrida, and Velinus nodipes undoubtedly propose the possible utilization of nad1-nad2 region as distinguishable molecular marker.","PeriodicalId":15069,"journal":{"name":"journal of biopesticides","volume":"3 2","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41286173","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Y. Sanjaya, Suhara Suhara, M. Halimah, Niken Diarti
{"title":"TOXICITY OF DERRIS ELIPTICA BENTH. ON MORTALITY OF AEDES AEGYPTI LINN LARVAE","authors":"Y. Sanjaya, Suhara Suhara, M. Halimah, Niken Diarti","doi":"10.57182/jbiopestic.15.2.118-121","DOIUrl":"https://doi.org/10.57182/jbiopestic.15.2.118-121","url":null,"abstract":"The death of the three Aedes aegypti Linn larvae that were placed inside has been the subject of a study on the toxicity test of Derris elliptica (Benth). This study's objectives were to ascertain the effect of tuba root extract on the quantity of larval instars of three Aedes aegypti Linn, as well as to ascertain the concentration of tubal root extract that affected as larvaside by measuring Lethal Concentration (LC50). Experiment-based research is the type used. With seven concentrations and three replications over the course of 24 hrs, the research was carried out in partnership using a Randomized Complete Random Design (RAL) design. The probit analysis yields the LC50 result. The findings demonstrated that Derris eliptica extract, with an LC50 value of 125.99 ppm, had an impact on the death of instar larvae of three Ae. aegypti Linn.","PeriodicalId":15069,"journal":{"name":"journal of biopesticides","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"49135786","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
H. Kutinkova, V. Dzhuvinov, N. Palagacheva, I. Staneva, S. Gandev, G. Kornov, M. Tityanov
{"title":"BIOLOGICAL CONTROL OF CODLING MOTH, CYDIA POMONELLA (L.) IN APPLE ORCHARDS OF BULGARIA USING GINKO® DISPENSERS","authors":"H. Kutinkova, V. Dzhuvinov, N. Palagacheva, I. Staneva, S. Gandev, G. Kornov, M. Tityanov","doi":"10.57182/jbiopestic.15.2.103-109","DOIUrl":"https://doi.org/10.57182/jbiopestic.15.2.103-109","url":null,"abstract":"The codling moth, Cydia pomonella (L.) (Lepidoptera: Tortricidae), is a significant pest of pome fruit and walnut orchards worldwide and it is a particular problem in Bulgaria. Codling moth resistance to many insecticides has been recently detected in Bulgaria. Codling moth (CM) mating disruption with Ginko® dispensers was evaluated in four successive years from 2019 to 2022 as part of an anti-resistance strategy. In an isolated 3 ha apple orchard in the Plovdiv district of South-Central Bulgaria, close to the town of Perushtitsa, field tests were conducted for the control of the codling moth (CM), C. pomonella, via mating disruption (MD) using pheromone Ginko® dispensers. The pheromone dispensers were hung in the upper third of tree canopies with a density of 500 dispensers/ha before CM flight started. Dynamics of CM flights was monitored by pheromone traps installed in the trial plot and in a conventionally treated reference orchard. Two types of baits were used in the traps: PHEROCON® CM L2–codlemone lures and PHEROCON® CM DA COMBO-P + AA lures (Trécé Inc., USA) in the orchards for mating disruption. The PHEROCON® CM L2 lures were changed at 4-week intervals and PHEROCON® CM DA COMBO-P + AA lures at 8-week intervals. PHEROCON® VI Delta sticky traps baited with PHEROCON CM DA COMBO- P + AA lures and standard CM L2 caps were installed, for comparison, in a reference orchard located in the Plovdiv region. Fruit infestation was periodically assessed till the harvest time. In both years fruit damage remained lower till late July and increased slightly only in August. At harvest, fruit damage remained below 1%. It was obvious that mating disruption could be useful strategy for the control of codling moth in Bulgarian apple orchards, specifically when the plot is isolated from external sources of infestation and the population density of the pest is low.","PeriodicalId":15069,"journal":{"name":"journal of biopesticides","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"43277561","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}