用蚯蚓堆肥生物强化的绿僵菌对镰孢菌（Fusarium oxysporum f.sp.LYCOPERSICI

Q3 Agricultural and Biological Sciences

journal of biopesticides Pub Date : 2024-06-01 DOI:10.57182/jbiopestic.16.1.24-32

R. N. Raman, K. V. Kumar, Prahlad Masurkar, Angel Jemima

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引用次数: 0

摘要

形态特征不足以准确划分毛霉属的不同物种。分子特征与形态特征相结合，可用于鉴定毛霉的种或亚种。本研究的重点是根据形态学和使用 ITS 等基因进行的分子分析来鉴定毛霉菌（Trichoderma asperellum）。光镜观察结果表明，Trichodermaasperellum 的分生孢子梗有规则的分枝，通常成对，具有直的分生孢子梗和球形至近球形的分生孢子。与 NCBI 数据库中的参考 T. asperellum 分离物进行的序列相似性分析表明，ITS4 和 ITS5 的核苷酸相似度为 100%。双培养试验清楚地表明，Trichoderma asperllum 能抑制被测真菌植物病原体 Fusarium oxysporum f. sp. lycopersici（87.38%）。在混合后 21 天内，发现未堆肥毛霉的种群动态一直保持稳定。

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EVALUATION OF TRICHODERMA ASPERELLUM BIOFORTIFIED WITH VERMI COMPOST AGAINST FUSARIUM OXYSPORUM F.SP. LYCOPERSICI

Morphological features are not adequate to accurately categorizedifferent species of the genus Trichoderma. Molecular characters, incombination with morphological characters, are used to identify Trichodermaat the species or subspecies level. The present study was focused oncharacterising Trichoderma asperellum based on morphology and molecularanalysis using genes such as ITS. Light microscopy results showed that Trichodermaasperellum regularly branched and typically paired conidiophores withstraight phialides and globose to subglobose shaped conidia Sequence similarityanalysis with reference T. asperellum isolates available in the NCBIdatabase showed 100 percent nucleotide similarity for ITS4 and ITS5. A dualculture test clearly showed that Trichoderma asperllum inhibited thetested fungal plant pathogen, Fusarium oxysporum f. sp. lycopersici(87.38 percent). Population dynamics of Trichoderma asperellum incompost were found to be stable until 21 days after mixing.

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来源期刊

journal of biopesticides Agricultural and Biological Sciences-Agronomy and Crop Science

CiteScore

0.80

自引率

0.00%

发文量