{"title":"EFFICACY OF IMIDACLOPRID AND BEAUVERIA BRONGNIARTII ON GREEN PEACH APHID, MYZUS PERSICAE, AND ITS PREDATOR, COCCINELLA UNDECIMPUNCTATA IN TOMATO","authors":"M. Sabbour, E. Shaurub","doi":"10.57182/jbiopestic.13.2.167-176","DOIUrl":"https://doi.org/10.57182/jbiopestic.13.2.167-176","url":null,"abstract":"Imidacloprid and the fungus Beauveria brongniartii (1x102 to 1x108 conidia/ml) were evaluated against the green peach aphid, Myzus persicae (second instar) and their predator Coccinella undecimpunctata (day-old adults) in tomato plantations in two different climatic Governorates Dakahlia and EL Behira. Using a spray technique to evaluate contact effect and a feeding technique to evaluate oral toxicity. Our results showed that under laboratory conditions, the LC50 values of Imidacloprid and B. brongniartii were 66 ppm and 72 conidia /ml, respectively against M. persicae. Under field conditions, the percentages of infested plants with M. persicae were significantly decreased after treatments with both Imidacloprid and B. brongniartii as compared with the corresponding controls. In El- Esraa Nobaryia (Behira) and El Dakahlia, the weights of tomato yield were 4978± 74.31 kg/feddan (F) and 4597±65.12 Kg/F in Imidacloprid plots treatments. The percentages of yield loss ranged between 24, 49 and 22, 50 in El- Esraa Nobaryia (Behira) and El Dakahlia, respectively. The study showed that C. undecimpunctata exhibit relatively high and reasonable resistance to Imidacloprid and Beauveria brongniartii at their highest lethal concentration for tested prey.","PeriodicalId":15069,"journal":{"name":"journal of biopesticides","volume":"1 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2020-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"43017452","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"ON FIELD MANAGEMENT OF ROOT-KNOT INFESTATION IN TOMATO AND BRINJAL THROUGH BIOFORMULATIONS OF PAECILOMYCES LILACINUS AND POCHONIA CHLAMYDOSPORIA","authors":"Swati Deep, M. Muslim, Nitul Tyagi, V. Prakash","doi":"10.57182/jbiopestic.13.2.187-194","DOIUrl":"https://doi.org/10.57182/jbiopestic.13.2.187-194","url":null,"abstract":"Bioformulations like wettable powder, aqueous liquid and granules of Paecilomyces lilacinus (Nematofree) and Pochonia chlamydosporia (Nematofree+) were evaluated against root-knot infestation in tomato and brinjal at two farmer’s field. The application of the bio-nematicides were carried out at transplanting stage of the crops as root dipping (@ 10gm or ml/ 1L of water) and soil treatment (@5Kg or L/acre incorporated with FYM).The root-knot infestation data was recorded by observing the foliage symptoms of root-knot infestation i.e., stunting, premature wilting, and leaf chlorosis. All the six treatments i.e., T1: Nematofree (WP), T2: Nematofree (Liquid), T3: Nematofree (Granule), T4: Nematofree+ (WP), T5: Nematofree+ (Liquid), T6: Nematofree+(Granule) showed significant reduction in the root-knot nematode infestation in both tomato crop (90 to 96%) and brinjal (75 to 86%). Among all formulation types, the wettable powder formulation of both bio-nematicides provided promising result in reduction of root-knot infestation symptoms. Nematofree+ was found to be more effective than Nematofree in controlling the infestation. Application of both the bionematicides also increased the final yield in case of both tomato and brinjal.","PeriodicalId":15069,"journal":{"name":"journal of biopesticides","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2020-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"43839900","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"INSECTICIDAL EFFECT OF THREE ESSENTIAL OILS AND BEAUVERICIN NANO GEL ON SITOPHILUS ORYZAE AND SITOPHILUS GRANARIUS (COLEOPTERA :CURCULIONIDAE)","authors":"M. Sabbour","doi":"10.57182/jbiopestic.13.2.127-134","DOIUrl":"https://doi.org/10.57182/jbiopestic.13.2.127-134","url":null,"abstract":"Sitophilus oryzae and Sitophilus granarius (Coleoptera :Curculionidae) harmful insects cause a damages to stored product seeds. These pests controlled by chemical materials which pollute the environments. Essential oils are a natural compound used as insecticide to a lot of pests. In our research we controlled these pests by essential oils, Cinnamomum zeylancium, Zingiber officinale, Cuminum cyminum and Beauvericin nano gel 2%. Results showed that, the LC50s of S. Oryzae were 38, 44, 151 and 18 ppm for Beauvericin nano gel 2% C. zeylancium, C. cyminum and Z. officinale, respectively. Also LC50s for S. granarius obtained by the corresponding treatments were, 41, 144, 11and 29ppm. The accumulative of S. oryzae significantly increased after 48 (H) by 2.8 fold after application of Cinnamomum zeylancium oil. When Zingiber officinale Oil applications the accumulative mortality increased by 3,3fold. The Beauvericin nano gel 2% showed that the accumulative increased by increasing the exposure time.Cinnamomum zeylancium showed a higher effect on the wheat weevil S. granarius, where the accumulative mortality recorded 91 after 48 (H). The lower accumulative mortality after Beauvericin nano gel 2%, recorded 38 after 48(H). The repellency test showed that, the highest repellant found among the Beauvericin nano gel 2% treatments. Data presented show that the persistence effectiveness of oil significantly decreased by increasing the storage time. The potential of the insecticidal oils was high after 45 days of storage, against S. oryzae and S. granarius. The potential of the insecticidal effect of oil recorded the lowest percentage at the end of storage after 100 days to both S. oryzae and S. granarius.","PeriodicalId":15069,"journal":{"name":"journal of biopesticides","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2020-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"48356674","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"EFFECTS OF PLANT EXTRACTS AND CHITOSAN AGAINST ALTERNARIA LEAF SPOT OF CHINESE KALE","authors":"Udomsri Ounchokdee, T. Dethoup","doi":"10.57182/jbiopestic.13.2.159-166","DOIUrl":"https://doi.org/10.57182/jbiopestic.13.2.159-166","url":null,"abstract":"Ethanol extracts of Alpinia galanga (L.)Willd. (rhizome), Coscinium fenestratum (Goetgh.) Colebr. (stem), Piper betle Linn. (leave), Piper longum L. (fruit), Zingiber cassumunar Roxb.(rhizome) and chitosan were evaluated for their fungicidal activities in controlling Alternaria leaf spot of Chinese kale, caused by Alternaria brassicicola, under field conditions. The results showed that the application of these plant extracts resulted in reduction of disease severity in a concentration-dependent manner. Among the five plant extracts tested, the extracts of P. betle and C. fenestratum at a concentration of 10,000 ppm displayed potent fungicidal activity in the suppression of disease severity, causing 43% and 41% disease reduction, respectively. The extracts of Z. cassumunar and A. galanga at 10,000 ppm showed moderate effectiveness in controlling the disease, causing 28% and 25% disease reduction, respectively, whereas the extract of P. longum at the same concentration possessed the lowest antifungal activity, reducing the disease severity by less than 20% at the highest dosed tested. The application of chitosan at 2% had a moderate effect; it reduced the disease severity by 30%. However, the application of the systemic fungicide, iprodione, displayed the greatest fungicidal activity in suppression disease severity, causing 65% disease reduction. The results in this study indicated that the P. betle and C. fenestratum may be promising eco-friendly candidates for controlling Alternaria leaf spot disease in Chinese kale production.","PeriodicalId":15069,"journal":{"name":"journal of biopesticides","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2020-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"47345235","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"EFFECT OF ENTOMOPATHOGENIC FUNGI BEAUVERIA BASSIANA ON THE CELLULAR IMMUNITY AND BIOCHEMISTRY OF GREEN BUG NEZARA VIRIDULA L.","authors":"A. Gad, M. Nada","doi":"10.57182/jbiopestic.13.2.135-144","DOIUrl":"https://doi.org/10.57182/jbiopestic.13.2.135-144","url":null,"abstract":"Entomopathogenic fungi (EPF) are considered effective methods for investigating insects’ immunity in response to infection. Insects have a variety of defense mechanisms against infectious diseases, namely phenoloxidase, hemocytes, detoxification and antioxidant enzymes.The instinctive immune system of insects is highly advanced and depends on humoral and cellular elements. In this study, five haemocytes types were found in fifth nymphal instar and adults of green bug, Nezara viridula (Heteroptera: Pentatomidae) namely; prohaemocyte (pr),granulocyte (gr), spherulocyte (sp), plasmatocyte (pl), coagulate cells (co) and oenocytoid (oe). Infection with Beauveria bassiana significantly decreased the different haemocytes count in both fifth nymphal instar and adults, especially after 72h post-infection. The haemocytes’ response against fungi was also investigated. A significant increase in Alpha esterase (EST), Acid phosphatise (ACP) and Alkaline phosphatise (ALP) were observed after 72h post infection in the haemolymph of N. viridula adults. In contrast, phenoloxidase activity significantly decreased in adults’ hemolymph after the same period of infection. Results indicated that infection with B. bassania affects the number of circulating haemocytes and decreases phenoloxidase activity.","PeriodicalId":15069,"journal":{"name":"journal of biopesticides","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2020-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"45040851","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"STUDY OF ANTIFUNGAL ACTIVITY OF LEAF EXTRACT OF SOME WILD PLANTS OF ASTERACEAE ON ASPERGILLUS FLAVUS","authors":"S. Gupta, Uttam Singh","doi":"10.57182/jbiopestic.13.2.145-149","DOIUrl":"https://doi.org/10.57182/jbiopestic.13.2.145-149","url":null,"abstract":"Aflatoxins have great significance due to health hazards to men and other useful animals. The aflatoxins are produced by Aspergillus flavus and A. parasiticus. Recently, the use of natural products like cinnamon and clove oil has also been suggested for the inhibition of aflatoxin production as well as growth of A. parasiticus. In view of the known antifungal properties of plant extracts, an attempt has been made in the present investigation to explore the Antifungal Activity of Plant extracts against growth of A. flavus by some wild plants of family Asteraceae, viz., Ageratum conyzoides, Carthamus oxycantha, Eclipta alba, Echinops echinatus, Launea nudicaulis, Lactuca dissecta, Parthenium hysterophorus, Sonchus aspera, Vernonia cinerea and Xanthium strumarium have been studied against growth of toxigenic strain of A. flavus by standard cup method on solid medium. The diameter of zone of inhibition was measured in mm and percent inhibition in growth was calculated. Out of three concentrations viz., 10, 15 and 20 per cent of leaf extracts, the 20 per cent concentration was found most effective. Further, hot water extract of leaf was adjudged more inhibitory as compared to cold water extract of plants. The maximum inhibition (70%) in growth of test fungus was caused by hot water leaf extract of A. conyzoides. It also can be concluded that leaf extract of different experimental plants have antifungal activity to varying extents.","PeriodicalId":15069,"journal":{"name":"journal of biopesticides","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2020-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"46060631","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"MONITORING THE TOXICITY OF DRY WOOD TERMITES USING THREE PLANT POWDERS IN LABORATORY CULTURE","authors":"Ojianwuna C.C., Enwemiwe V.N,","doi":"10.57182/jbiopestic.13.2.150-158","DOIUrl":"https://doi.org/10.57182/jbiopestic.13.2.150-158","url":null,"abstract":"In order to model a suitable control alternative for subterranean termites and termites infesting wooden furniture and cabinets where consumables are likely stored, this present study monitored the efficacy of three plant powders at different doses (0.50-1.50g) against the dry wood termite,Cryptotermes cavifrons Banks (Blattodea). Plant materials were locally prepared into powders and assigned to 20 wood termites in 5g substrates of sand and stick. The culture was set up in triplicates and lethal concentrations were determined by Probit analysis. The plant powder could not exerted 100% mortality, but the highest mortality was recorded in Dennettia tripetala (1.50g) on stick (mean= 8.33±0.80). All concentration of D. tripetala on sand and stick caused mortality within 0 to 48 hours after exposure and compared favorably with Permethrin which recorded the highest mean time mortality on sand at 0-24 hrs after exposure. The lowest LC50value (0.75 and 0.79g/g) corresponded to Permethrin and D. tripetala on sand and stick respectively. This results suggest that best concentrations of pepper fruit powder can be adopted as potential alternative to chemical powders.","PeriodicalId":15069,"journal":{"name":"journal of biopesticides","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2020-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"43042056","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"DISRUPTIVE EFFECT OF PYROGALLOL ON DEVELOPMENT OF SPODOPTERA LITURA (FAB.) LARVAE","authors":"N. Singh Chauhan, Satwinder Kaur Sohal","doi":"10.57182/jbiopestic.11.1.07-13","DOIUrl":"https://doi.org/10.57182/jbiopestic.11.1.07-13","url":null,"abstract":"In an endeavour to explore secondary metabolites as an important and safe means of pest management, we investigated the effects of pyrogallol, a phenolic compound, on the growth and development of Spodoptera litura (Fab.). It is a serious pest of a large number of economically important crops. Different concentrations viz., 1, 5, 25, 125, 625, 3125 ppm of pyrogallol were incorporated in artificial diet of larvae and the antibiosis influence was ascertained by feeding second instar larvae on this diet. The larval and total development period increased significantly with increase in concentration of the pyrogallol. Inhibitory effects of the compound were observed on per cent pupation, per cent emergence and adult longevity. All nutritional indices were also significantly affected. Thus it can be considered as a promising substance to be used as a biopesticide against the insect pest.","PeriodicalId":15069,"journal":{"name":"journal of biopesticides","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2018-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"48125578","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Eka Candra Lina, Indah Widhianingrum, Mita Eka Putri, Nur Fni Evalia, M. Makky
{"title":"INSECTICIDAL ACTIVITY OF PIPER ADUNCUM FRUIT AND TEPHROSIA VOGELII LEAF MIXED FORMULATIONS AGAINST PLUTELLA XYLOSTELLA (L.) (LEPIDOPTERA: PLUTELLIDAE)","authors":"Eka Candra Lina, Indah Widhianingrum, Mita Eka Putri, Nur Fni Evalia, M. Makky","doi":"10.57182/jbiopestic.11.1.69-75","DOIUrl":"https://doi.org/10.57182/jbiopestic.11.1.69-75","url":null,"abstract":"The emulsifiable concentrate (EC) and wettable powder (WP) of Piper aduncum and Tephrosia vogelii mixed formulations were tested for their activities in the laboratory and their effectiveness in the field against cabbage pest Plutella xyostella. Cabbage leaves soaked in six different mixed formulation concentrations were tested against second instar larvae of P. xylostella and observed until the larvae reached fourth instar stage. From the survived larvae, growth and development, antifeedant effects were recorded. Field efficacy test was based on LC95 value of formulations from laboratory test result. The experiment used a randomized block design with 5 treatments and 3 replications to determine the insecticide effectivity against populations of P. xylostella larvae. The results showed that the EC and WP mixed formulations have insecticidal activity against P. xylostella larvae, with LC95 value of 0.35% and 0.37%, respectively. The highest antifeedant effect on EC mixed formulation was 85.01% and WP mixed formulation was 86.23%. Both mixed formulations also slowed the development of larvae when compared with control. Field effication result showed that applications of EC mixed formulation were able to restrain the population of P. xylostella, with effectivity value of 71.06%. Insecticide effectivity value of EC mixed formulation was higher than WP mixed formulation and Bacillus thuringiensis formulation.","PeriodicalId":15069,"journal":{"name":"journal of biopesticides","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2018-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"49414848","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"EVALUATION OF BT LIQUID FORMULATIONS AGAINST GRAM POD BORER, HELICOVERPA ARMIGERA (HUBNER) AND SPOTTED POD BORER, MARUCA VITRATA (GEYER) IN PIGEONPEA","authors":"M. Sreekanth, M. Seshamahalakshmi","doi":"10.57182/jbiopestic.11.1.52-59","DOIUrl":"https://doi.org/10.57182/jbiopestic.11.1.52-59","url":null,"abstract":"A field experiment was conducted during 2012-15 at Regional Agricultural Research Station, Lam, Guntur district, Andhra Pradesh to evaluate the efficacy of Bt liquid formulations and other biopesticides against gram pod borer, Helicoverpa armigera and spotted pod borer, Maruca vitrata in pigeonpea. There were nine treatments [2 strains of Bt liquid formulations each at two doses, two doses of Beauveria bassiana, neem formulation (azadirachtin 1500 ppm), and chemical check] including untreated control. Pooled analysis of three years data revealed that two sprays of NBAII BtG4 @ 2% at fortnight interval significantly superior over other treatments in suppressing the larval population of H. armigera (3.2 larvae / plant) and M. vitrata (5.9 larvae / plant) on pigeonpea and recorded minimum pod damage (3.7 and 11.4%, respectively) with maximum yield (1565 kg/ha). Further, it was also revealed that all Bt liquid formulations and B. bassiana were safe to natural enemy population viz., spiders and coccinellids existing in pigeonpea ecosystem. It is suggested to utilize biopesticides as ecofriendly insecticides for pigeonpea production.","PeriodicalId":15069,"journal":{"name":"journal of biopesticides","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2018-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"44484852","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}