Journal of Bacteriology & Parasitology最新文献_第10页

Bacterial infections and cancer therapy 细菌感染和癌症治疗

Journal of Bacteriology & Parasitology Pub Date : 2017-06-01 DOI: 10.4172/2155-9597-C1-027

An, A. Chakrabarty

引用次数: 0

Prevalence and Risk Factors of Cryptosporidium Infection in ChildrenHospitalized for Diarrhea in Guangzhou, China 广州地区腹泻住院儿童隐孢子虫感染流行及危险因素分析

Journal of Bacteriology & Parasitology Pub Date : 2017-05-17 DOI: 10.4172/2155-9597.1000308

Shouyi Chen, E. Atwill, F. Zhong, Yue-hong Wei, Shui-ping Hou, Jun-tao Li, Conghui Xu, Chengling Xiao, Zhicong Yang, Xunde Li

{"title":"Prevalence and Risk Factors of Cryptosporidium Infection in ChildrenHospitalized for Diarrhea in Guangzhou, China","authors":"Shouyi Chen, E. Atwill, F. Zhong, Yue-hong Wei, Shui-ping Hou, Jun-tao Li, Conghui Xu, Chengling Xiao, Zhicong Yang, Xunde Li","doi":"10.4172/2155-9597.1000308","DOIUrl":"https://doi.org/10.4172/2155-9597.1000308","url":null,"abstract":"This study was conducted to determine the prevalence, species and risk factors of Cryptosporidium infection in children hospitalized for diarrhea in Guangzhou region of China. A cross-sectional study was conducted to assess the prevalence and risk factors of Cryptosporidium infection in children (2 weeks to 10 years old) who were hospitalized for diarrhea. Cryptosporidium oocysts were detected using direct immunfluorescent assay and species were determined by sequencing a fragment (~800 bp) of the 18S rRNA gene. A questionnaire contains host, sociodemographic, family, hygiene, diet, zoonotic, and environmental risk factors was administered to patients to identify key factors associated with infections. The observed Cryptosporidium prevalence was 6.9% and the true prevalence was estimated to be 9.0%. Cryptosporidium infection was similar between male (7.4%) and female (6.1%) children and negatively associated with age (i.e., infection was more likely in younger children). Infections in children were significantly associated with family members’ diarrhea within past one month. Infection in children in suburban hospitals (7.8%) was significantly higher than that in urban hospitals (2.1%). The overall prevalence of Cryptosporidium in rainy season was significantly higher than that in non-rainy seasons. DNA sequences of the 18S rRNA gene from infected children were 99.12% to 100% identical to sequences in the GenBank of C. parvum isolates from humans and animals. Future works should determine the sources of zoonotic Cryptosporidium and routes of waterborne exposure in the rainy season in this region.","PeriodicalId":15045,"journal":{"name":"Journal of Bacteriology & Parasitology","volume":"22 1","pages":"1-8"},"PeriodicalIF":0.0,"publicationDate":"2017-05-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"86978647","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 3

Epidemiology of Tinea Capitis and Associated Factors among School AgeChildren in Hawassa Zuria District, Southern Ethiopia, 2016 2016年埃塞俄比亚南部Hawassa Zuria地区学龄儿童头癣流行病学及相关因素分析

Journal of Bacteriology & Parasitology Pub Date : 2017-05-17 DOI: 10.4172/2155-9597.1000309

D. T. Hibstu, D. Kebede

{"title":"Epidemiology of Tinea Capitis and Associated Factors among School AgeChildren in Hawassa Zuria District, Southern Ethiopia, 2016","authors":"D. T. Hibstu, D. Kebede","doi":"10.4172/2155-9597.1000309","DOIUrl":"https://doi.org/10.4172/2155-9597.1000309","url":null,"abstract":"Introduction: Africa, being the settings mostly affected, the rates of tinea infection ranges between 10 and 30% among school-aged children. For instance in Ethiopia, the prevalence of tinea capitis among school children was 47.5%. The aim of this study was to determine the magnitude of tinea capitis and associated factors among school age children in Dorebafano town, Hawassa Zuria Disrict, Southern Ethiopia. Methods: A community based cross-sectional study design was conducted among 292 children in Dorebafano town from June 30 to July 6, 2016 using systematic random sampling technique. Data was collected using pretested and interviewer administered structured questionnaire. The presence or absence of tinea capitis was confirmed by physical examination and taking samples from scalp scratch and observing under a microscope with potassium hydroxide (KOH). Logistic regression was carried out to identify factors associated with presence of tinea capitits. Model fitness was checked using Hosmer and Lemeshow show. Result: The magnitude of tinea capitis among the study subjects was 32.3% [CI: 27.3%-37.5%]. Age of child (AOR=3.2, 95% CI: 1.40, 7.00), sex of child (AOR=0.10, CI (0.03, 0.40), educational status of the child (AOR=6.9, 95% CI: 1.4, 33.5) and presence of similar illness (AOR=6.49, 95% CI: 2.42, 17.43) were identified to be independent factors for the occurrence of tinea capitis. Conclusion: The magnitude of tinea capitis among the study subjects was found to be high. Age, sex, educational status of children and presence of similar illness were identified factors for occurrence of tinea capitis. Health promotion, health education measures and early identification and treatment of tinea capitis need to be given emphasis.","PeriodicalId":15045,"journal":{"name":"Journal of Bacteriology & Parasitology","volume":"3 1","pages":"1-5"},"PeriodicalIF":0.0,"publicationDate":"2017-05-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"86898652","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 3

Identification of NuoX and NuoY Ligand Binding Specificity in theCampylobacter Jejuni Complex I 空肠弯曲杆菌NuoX和NuoY配体结合特异性的鉴定

Journal of Bacteriology & Parasitology Pub Date : 2017-04-26 DOI: 10.4172/2155-9597.1000307

L. I. Calderon-Gomez, C. Day, L. Hartley-Tassell, Jennifer C. Wilson, G. Mendz, V. Korolik

{"title":"Identification of NuoX and NuoY Ligand Binding Specificity in theCampylobacter Jejuni Complex I","authors":"L. I. Calderon-Gomez, C. Day, L. Hartley-Tassell, Jennifer C. Wilson, G. Mendz, V. Korolik","doi":"10.4172/2155-9597.1000307","DOIUrl":"https://doi.org/10.4172/2155-9597.1000307","url":null,"abstract":"The components of the proton pump NADH:ubiquinone (Complex I) of the respiration pathway have been identified in the C. jejuni genome. However, the paradigm genes nuoE and nuoF encoding subunits of the NADH dehydrogenase module of Complex I are absent. Instead the genes cj1575c and cj1574c encoding NuoX and NuoY are present in the loci corresponding to nuoE and nuoF, respectively. Bioinformatics analyses showed the presence of nuoX and nuoY homologues in all sequenced strains of C. jejuni and in other Campylobacter species, as well as the presence of orthologues in other E-Proteobacteria. To understand the involvement of the NuoX and NuoY proteins in the respiration of C. jejuni and to characterize their ligand binding specificity and affinity, a tricarboxylic acid cycle array was developed as a tool to identify proteins that can bind to intermediates of this cycle as well as other metabolites. This array showed that NuoX bound FAD2+, and NuoY bound FAD2+ and the electron donors malate and lactate. Saturation Transfer Difference Nuclear Magnetic Resonance studies confirmed the NuoY binding ligands, and suggested that the flavin moiety of FAD2+ interacted more strongly with NuoY than the adenine moiety. Affinity data generated by Surface Plasmon Resonance indicated that NuoY bound to FAD2+ with a KD of 337 nM; NuoX and NuoY had an affinity for NADH of a KD of 403 nM and 478 nM, respectively, and a ten-fold lower affinity for both NAD+ and FAD2+. The data suggested that the flavin-adenine dinucletoide could be bound preferentially to the NAD in the Complex I of C. jejuni.","PeriodicalId":15045,"journal":{"name":"Journal of Bacteriology & Parasitology","volume":"33 1","pages":"1-8"},"PeriodicalIF":0.0,"publicationDate":"2017-04-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"89808567","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Therapeutic DNA Vaccines: The Final Step for Success 治疗性DNA疫苗:成功的最后一步

Journal of Bacteriology & Parasitology Pub Date : 2017-04-03 DOI: 10.4172/2155-9597.1000E125

F. D. Carmo, R. Carvalho, G. Jan, V. Azevedo

引用次数: 2

Lyme Disease Bacterium, Borrelia burgdorferi Sensu Lato, Detected inMultiple Tick Species at Kenora, Ontario, Canada 加拿大安大略省基诺拉市多种蜱类中检测到莱姆病细菌伯氏疏螺旋体

Journal of Bacteriology & Parasitology Pub Date : 2017-03-20 DOI: 10.4172/2155-9597.1000304

John D. Scott, K. Clark, John F Anderson, J. Foley, Monica R. Young, L. Durden

{"title":"Lyme Disease Bacterium, Borrelia burgdorferi Sensu Lato, Detected inMultiple Tick Species at Kenora, Ontario, Canada","authors":"John D. Scott, K. Clark, John F Anderson, J. Foley, Monica R. Young, L. Durden","doi":"10.4172/2155-9597.1000304","DOIUrl":"https://doi.org/10.4172/2155-9597.1000304","url":null,"abstract":"We detected the Lyme disease bacterium, Borrelia burgdorferi sensu lato (s.l.), in 8 species of ixodid ticks (Acari: Ixodidae) collected from mammalian hosts, including humans, at Kenora, Ontario, Canada. These 8 tick species include Ixodes angustus, Ixodes banksi, Ixodes cookei (groundhog tick), Ixodes gregsoni, Ixodes muris (mouse tick), Ixodes scapularis (blacklegged tick), Haemaphysalis leporispalustris (rabbit tick), and Dermacentor albipictus (winter tick). Based on PCR amplification, 39 (41%) of 94 ticks tested were positive for B. burgdorferi s.l. DNA sequencing of the flagellin B (flaB) gene of B. burgdorferi s.l. complex revealed the presence of B. burgdorferi sensu stricto (s.s.), which is pathogenic to humans, and causes diverse neurological manifestations in patients. Notably, we provide the first record of B. burgdorferi s.l. in I. gregsoni, and reveal a new distribution record for this tick in eastern and central Canada by extending the known range westward by 200 km. Our findings indicate that there may be a wide-ranging enzootic transmission cycle of B. burgdorferi s.l. within the ecosystem throughout the Kenora area. The health-care profession must be fully cognisant that Lyme disease is present in the Kenora area, and is a public health risk.","PeriodicalId":15045,"journal":{"name":"Journal of Bacteriology & Parasitology","volume":"114 1","pages":"1-10"},"PeriodicalIF":0.0,"publicationDate":"2017-03-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"77695140","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 13

A Survey of Helminth Parasites of the Lizard, Agama agama in IleÃ¢ÂÂIfe andIbadan Southwest Nigeria 尼日利亚西南部IleÃ ÂÂIfe和dibadan地区蜥蜴Agama Agama的寄生虫调查

Journal of Bacteriology & Parasitology Pub Date : 2017-03-13 DOI: 10.4172/2155-9597.1000303

O. Sowemimo, T. A. Oluwafemi

引用次数: 10

A Follow up Study of Bacteriology and Antibiotic Sensitivity Pattern of Urinary Tract Infection in a Tertiary Care Hospital in Bangladesh 孟加拉国某三级医院尿路感染细菌学及抗生素敏感性的随访研究

Journal of Bacteriology & Parasitology Pub Date : 2017-01-01 DOI: 10.4172/2155-9597.1000334

M. I. Majumder, T. Ahmed, N. Sakib, A. Khan, C. Saha

{"title":"A Follow up Study of Bacteriology and Antibiotic Sensitivity Pattern of Urinary Tract Infection in a Tertiary Care Hospital in Bangladesh","authors":"M. I. Majumder, T. Ahmed, N. Sakib, A. Khan, C. Saha","doi":"10.4172/2155-9597.1000334","DOIUrl":"https://doi.org/10.4172/2155-9597.1000334","url":null,"abstract":"Background: Urinary tract infections (UTIs) are a common infection. The aim of this follow up study is to see the changing trends in bacteriology and antibiotic sensitivity pattern among uropathogens in comparison to similar study 5 years back. Materials and methods: We performed a prospective study in Comilla medical college hospital, Bangladesh during the period of July 2015-June 2016. Urine samples from 658 suspected UTI patients aged above 12 years inoculated for semiquantitative urine culture and sensitivity test. Antibiotic susceptibility pattern was done by Kirby- Bauer disc diffusion method following clinical laboratory science (CLS) program. Results: Culture passivity were in 198 samples among 658 inoculated samples. E. coli was isolated from 171(86%) samples which was the most predominant bacteria followed by Klebsiella and Enterococcus. UTI with E. coli was significantly increased in the year 2016 in comparison to 2011. Meropenem, imipenem, amikacin, tazobactum, gentamycin nitrofurantoin, and mecillinum, found sensitive against 88% to100% of the uropathogens. Bacteria offered high degree of resistance against commonly used antibiotics-amoxycillin, amoxiclav, cephradine and cefixime ranging 60% to 86%. Comparative study of 2016 vs. 2011 shows significant reduction in sensitivity for imipenum, ceftriaxone, amoxiclav and increased sensitivity for nalidexic acid, ciprofloxacin, mecillinum, colistin, cotrimoxazole. Conclusion: UTI with E. coli was significantly increased in the year 2016. This study failed to show a steady increase in resistance to all studied antibiotics. Imipenem, meropenem, tazobactum, amikacin and nitrofurantoin still remain more sensitive in this comparative study. Comparative study of 2016 vs. 2011 shows significant increasing resistance for imipenum, ceftriaxone, amoxiclav and increasing sensitivity for nalidexic acid, ciprofloxacin, mecillinum, colistin, cotrimoxazole.","PeriodicalId":15045,"journal":{"name":"Journal of Bacteriology & Parasitology","volume":"24 1","pages":"1-5"},"PeriodicalIF":0.0,"publicationDate":"2017-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"80799465","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 12

Disinfectant Use in the Hospital Environment for Microorganisms Control 消毒剂在医院环境微生物控制中的应用

Journal of Bacteriology & Parasitology Pub Date : 2017-01-01 DOI: 10.4172/2155-9597.1000326

Édilon Sembarski de Oliveira, E. Araujo, J. García, F. Agostinho, Karlla Kristinna Almeida Medeiros, T. Paulino, R. Paludo, M. Ribeiro, C. B. Miguel, W. Rodrigues

{"title":"Disinfectant Use in the Hospital Environment for Microorganisms Control","authors":"Édilon Sembarski de Oliveira, E. Araujo, J. García, F. Agostinho, Karlla Kristinna Almeida Medeiros, T. Paulino, R. Paludo, M. Ribeiro, C. B. Miguel, W. Rodrigues","doi":"10.4172/2155-9597.1000326","DOIUrl":"https://doi.org/10.4172/2155-9597.1000326","url":null,"abstract":"Studies indicate that the hospital has an important role in transmission of various pathogens. To minimize the spread of these microorganisms in hospitals, they proposed various forms of disinfection, however diversity and effectiveness of these methods are varied. Thus, this study aimed to understand the associations and approaches that have been reported in recent years on microorganisms control by disinfectants in the hospital environment, thus enabling the search for new strategies and/or implementation of conducts already performed in other hospitals. In this article they performed a systematic review on the topic, in accordance with the preferred reporting items for systematic reviews and meta-analyzes-PRISMA. Articles were selected published between the years 2012-2016 present in the PubMed database. The data demonstrate that reviews many conventional methods may be flawed (10%), or may not be able to reduce the number of microorganisms (30%). The most frequently related microorganisms were Clostridium difficile (20%), methicillin-resistant Staphylococcus aureus (40%), or Enterococcus resistant to vancomycin (20%). There was no evidence statistical differences for a tendency to exchange the conventional methods, however the management was highlighted by 50% of the disinfection process. But our approach has enabled better understanding of mechanisms linked to environmental disinfection process of pathogenic microorganisms, thus pointing to coherent strategies in disinfection processes, which have benefits with the reduction of major causative agents of nosocomial infections and thus with decreasing nosocomial diseases.","PeriodicalId":15045,"journal":{"name":"Journal of Bacteriology & Parasitology","volume":"37 1","pages":"1-5"},"PeriodicalIF":0.0,"publicationDate":"2017-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"77588654","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 2

Production of Chitosan/Zinc Oxide Complex by Ultrasonic Treatment with Antibacterial Activity 超声法制备壳聚糖/氧化锌配合物的研究

Journal of Bacteriology & Parasitology Pub Date : 2017-01-01 DOI: 10.4172/2155-9597.1000330

M. Barreto, C. T. Andrade, E. G. Azero, V. Paschoalin, E. Aguila

{"title":"Production of Chitosan/Zinc Oxide Complex by Ultrasonic Treatment with Antibacterial Activity","authors":"M. Barreto, C. T. Andrade, E. G. Azero, V. Paschoalin, E. Aguila","doi":"10.4172/2155-9597.1000330","DOIUrl":"https://doi.org/10.4172/2155-9597.1000330","url":null,"abstract":"This study was conducted with the objective of investigating the physical and antimicrobial properties of structured zinc oxide compounds in chitosan submitted to different times of ultrasonic treatment. Zinc oxide particles were dispersed in water and coated with medium molar mass chitosan and two other sonicated samples, following a simple methodology. Before drying, the chitosan/zinc oxide water suspensions were characterized by rheological tests. Their rheological behavior depended on the period of time during which the chitosan sample was submitted to ultrasound. After drying, the microparticles were characterized by infrared spectroscopy, atomic absorption spectrophotometry and scanning electron microscopy (SEM). The microparticles were investigated as for their particle size distribution (PSD). SEM and PSD results revealed that the chitosan/zinc oxide microparticles had a multimodal dispersion. The antibacterial activity of the neat zinc oxide nanoparticles and of the microparticles was evaluated against Escherichia coli and Staphylococcus aureus. The results showed that the ZnO nanoparticles had a lower minimum bactericidal concentration (500 μm/mL against E. coli and 650 μm/mL against S. aureus) than the chitosan-coated/ZnO microparticles. The microparticles with the lowest average particle size and the highest homogeneity exhibited the highest antibacterial activity against both bacteria. This result was attributed to the additional antibacterial activity of soluble zinc ions and of the chitosan sample.","PeriodicalId":15045,"journal":{"name":"Journal of Bacteriology & Parasitology","volume":"22 1","pages":"1-7"},"PeriodicalIF":0.0,"publicationDate":"2017-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"81004003","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 10