Ebimobowei Assayomo, D. Bawo, G. K. Dieware, Amaka Mariagoretti Buseri, Ebikabowei John Debekeme
{"title":"Determination of Heavy Metals in Soil around Amasoma Community in Bayelsa State, Nigeria","authors":"Ebimobowei Assayomo, D. Bawo, G. K. Dieware, Amaka Mariagoretti Buseri, Ebikabowei John Debekeme","doi":"10.9734/jabb/2021/v24i1030244","DOIUrl":"https://doi.org/10.9734/jabb/2021/v24i1030244","url":null,"abstract":"Aims: Anthropogenic activities such as crude oil exploration and exploitation has led to the suspicion of heavy metals contamination in the study area. This study investigated the concentration of zinc, nickel, lead and cadmium in the soil extracted from the area where Scent Leaf (Ocimum gratissimum) and Pawpaw tree (Carica papaya) are grown in Amassoma community, Bayelsa State, Nigeria. \u0000Study Design: Scent leaf (Ocimum gratissimum) and Pawpaw tree (Carica papaya) soils from where these plants are grown were randomly collected for heavy metal analysis from within Amassoma community in Southern Ijaw Local Government Area of Bayelsa State, Nigeria \u0000Place and Duration of Study: This study was carried out in Biological Sciences, Faculty of Science, Niger Delta University, Wilberforce Island, Amassoma, Bayelsa State between March 2019 to September 2019 \u0000Methodology: Scent leaf (Ocimum gratissimum) and Pawpaw tree (Carica papaya) soils were digested and analyzed for heavy metals using Atomic Absorption Spectrophotometer (AAS, model S471096). The method used was wet-ashing method for the analysis. \u0000Results: the concentrations of Lead (Pb), Nickel (Ni), Cadmium (Cd), and Zinc (Zn), in pawpaw soil and scent leaf soil. From the sample data analysis, the average lead (Pb) contents in pawpaw soil and scent leaf soil were 1.283 mg/kg and 1.44 mg/kg respectively. The average Cadmium (Cd) in pawpaw soil and scent leaf soil were 0.256 mg/kg and 0.256 mg/kg respectively. The average Nickel (Ni) compositions in pawpaw soil and scent leaf soil were 4.71 mg/kg and 5.09 mg/kg respectively. While the Average Zinc (Zn) in pawpaw soil and scent leaf soil is 2.280 mg/kg and 2.160 mg/kg respectively. The soil analyzed for heavy metals were below the threshold target values recommended by the WHO. \u0000Conclusion: The Scent leaf (Ocimum gratissimum) and Pawpaw tree (Carica papaya) soils analyzed for heavy metals were below the permissible limit recommended by WHO for soil safety. The result from the present study revealed that there may not be soil contamination as a result of the selected heavy metals, however, It is recommended that caution should be taken in regulating the anthropogenic activities that may tend to elevate the level of heavy metals in the surrounding to prevent contamination of soil over time which could be detrimental to the health of the populace.","PeriodicalId":15023,"journal":{"name":"Journal of Advances in Biology & Biotechnology","volume":"69 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-12-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"73020974","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Comparative Review of Plant Transformation Techniques","authors":"A. okpe, F. Nkaa","doi":"10.9734/jabb/2021/v24i1030243","DOIUrl":"https://doi.org/10.9734/jabb/2021/v24i1030243","url":null,"abstract":"Plant transformation is now an important biotechnological tool in plant biology and a practical tool for transgenic plant development. There are many verified methods for stable introduction of novel genes into the nuclear genomes of diverse plant species. As a result, gene transfer and regeneration of transgenic plants are no longer the factors limiting the development and application of practical transformation systems for many plant species. However, the desire for higher transformation efficiency has stimulated work on not only improving various existing methods but also in inventing novel methods. Different methods of transferring the gene into plant cells have been developed and continuous efforts have been made to increase its efficiency. Both direct and indirect methods of gene transfer have their own merits and demerits. Efforts have been made continuously to eliminate drawbacks and to develop an easy and eco-friendly method to transfer foreign genes. Many methods of genetic transformation have been proposed and tried in the laboratories, but most of them result to transient expressions. However, transformation work based on particle bombardment with DNA coated micro projectiles and Agrobacterium mediated transformation have proved to be promising in producing stable transgenic plants from a range of plant species.","PeriodicalId":15023,"journal":{"name":"Journal of Advances in Biology & Biotechnology","volume":"26 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-12-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"86667643","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Diarrassouba Moussa, Soumahin Eric Francis, Konan Djézou, N’guessan Kan Pulchérie, Moro Affia Perpétue, Koffi Antoine, Obouayeba Samuel
{"title":"Determination of the Influence of Cultural Techniques \"Densities and Opening Standardʺ on Agrophysiological Parameters of Clones Pb 260, Irca 111 and Rrim 703","authors":"Diarrassouba Moussa, Soumahin Eric Francis, Konan Djézou, N’guessan Kan Pulchérie, Moro Affia Perpétue, Koffi Antoine, Obouayeba Samuel","doi":"10.9734/jabb/2021/v24i930240","DOIUrl":"https://doi.org/10.9734/jabb/2021/v24i930240","url":null,"abstract":"To find out the effect of cultural techniques on agrophysiological parameters, four combinations ̏planting densities (low density or DF at 350 t/ha, normal density or DN at 510 t/ha) and opening standards (opening at 65cm and opening at 50 cm) ̋ were tested on Hevea brasiliensis clones PB 260, IRCA 111 and RRIM 703. This study was conducted for nine years with a split-plot experimental design of two treatments and two subtreatments repeated three times. The different combinations of treatments and subtreatments tested were low density (DN-50 cm), low density (DN-65 cm), normal density (DF-50 cm), normal density (DF- 65 cm). The parameters evaluated were the production at bleeding and per hectare, the average annual increase in circumference, the rate of tree losses and the rate of trees with dry notches. Production per tree was significantly higher at 350 t/ha and 65 cm opening (DF-65 cm), while per hectare production was higher at 510 t/ha. The girths of the different clones are stronger at DF and at the 65 cm opening. The rate of tree loss and the rate of trees with dry notch were low at the 510 t/ha density and the 65cm opening. The appropriate density and opening standard was \"normal density 510 t/ha and opening to circumference 50 cm\". The cultivation techniques especially the density and opening standard judiciously applied can participate in the sustainable improvement of rubber productivity of rubber plantations.","PeriodicalId":15023,"journal":{"name":"Journal of Advances in Biology & Biotechnology","volume":"29 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-12-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"88049152","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
N. Ogbuji, A. Ataga, P. Tari-Ukuta, C. J. Olisedeme
{"title":"Molecular Characterization of Fungi Associated with Dump Site Soil","authors":"N. Ogbuji, A. Ataga, P. Tari-Ukuta, C. J. Olisedeme","doi":"10.9734/jabb/2021/v24i930239","DOIUrl":"https://doi.org/10.9734/jabb/2021/v24i930239","url":null,"abstract":"Aims: A study was conducted to identify fungal species isolated from dumpsite soil in University of Port Harcourt using molecular techniques. \u0000Methodology: Molecular methods for determining the species of a fungus based on the amplification and sequencing of the internal subscribed spacer (ITS) region of the fungal rRNA operon using molecular markers was applied. Soil sample was collected from a dumpsite in the University of Port Harcourt, Rivers State, Nigeria. Isolation of fungi associated with the dumpsite soil was carried out using spread plate method. Fungal genomic DNA was extracted using Quick-DNA Fungal/Bacterial Miniprep kit. The ITS1-2 gene of the isolates was amplified by Polymerase Chain Reaction (PCR) using the primer pair; ITS4 and ITS5. \u0000Results: The sequences of the amplified ITS region were blasted against known sequences on the National Centre for Biotechnology Information (NCBI) database. Nucleotide sequence analysis revealed the species identity of the fungal isolates to be: Aspergillus fumigatus, Trichoderma harzianum, Aspergillus felis, Aspergillus templicola, Aspergillus flavipes, Aspergillus fumigatus and Cunninghamella binariae. Phylogenetic analysis was carried out to ascertain the relationship between the isolates and other closely-related isolates on GenBank. Isolates 2 (Trichoderma harzianum) and 7 (Cunninghamella binariae), 3 (Aspergillus felis) and 6 (Aspergillus fumigatus), and 4 (Aspergillus templicola) and 5 (Aspergillus flavipes) were found to be more closely related to each other. \u0000Conclusion: The molecular techniques employed successfully identified the organisms to the species level as these techniques are based on the genetic constitution of organisms. The result obtained from this study will complement the information on the fungal organisms associated with dumpsite soil.","PeriodicalId":15023,"journal":{"name":"Journal of Advances in Biology & Biotechnology","volume":"29 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-12-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"85321579","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Nazmul Alam Khan, M. Uddin, M. Rana, N. Jahan, Mirana Akhter Sumi, M. Rashid
{"title":"Callus Induction, Regeneration and Establishment of Rice Plant from Mature Embryo","authors":"Nazmul Alam Khan, M. Uddin, M. Rana, N. Jahan, Mirana Akhter Sumi, M. Rashid","doi":"10.9734/jabb/2021/v24i930238","DOIUrl":"https://doi.org/10.9734/jabb/2021/v24i930238","url":null,"abstract":"Plant growth regulators were used to test callus induction and in vitro regeneration in six rice genotypes (RM-AC-2, BRRI dhan89, BRRI dhan88, Nipponbare, Koshihikari and Zenshan97). Four different concentrations (1, 2, 3 and 4 mg/L) of 2,4-D for callus induction and three different concentrations (1,2 and 3 mg/L) of NAA with three doses (5,10 and 15 µ/L) of kinetin for callus regeneration were used to test the effect of plant growth regulators. This study found a high callus induction on MS medium enriched with 2 mg/L 2, 4-D. In cases of RM-AC-2, BRRI dhan89, BRRI dhan88, Nipponbare, Koshihikari and Zenshan97, callus induction frequencies were 92.7%, 87.8%, 84.6%, 82.9%, 86.2% and 62.9%, respectively. In the regeneration, it was found that an MS medium enriched with 2 g/L Kinetin and 10 µm/L NAA has the ability to induce increased regeneration of different rice varieties (RM-AC-2 (72.4%), BRRI dhan89 (66.9%), BRRI dhan88 (62.5%), Nipponbare (63.3%), Koshihikari (48%) and Zenshan97 (39.6%). From the regenerated plants, one plant of the RM-AC-2 genotype availed to complete its life cycle and generated 32 effective tillers and yielded 89g. This rice plant is very promising for high yielding rice variety development program in Bangladesh. The improved callus development and regeneration ability of this genotype might be helpful for future rice variety development and genetic transformation program.","PeriodicalId":15023,"journal":{"name":"Journal of Advances in Biology & Biotechnology","volume":"58 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-11-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"77530577","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Antimicrobial Activity and Toxicity of Sophorolipids Produced by Candida haemulonis and Saccharomyces cerevisiae against Some Selected Microorganisms","authors":"K. Williams, O. Agwa, G. Abu, O. Akaranta","doi":"10.9734/jabb/2021/v24i930234","DOIUrl":"https://doi.org/10.9734/jabb/2021/v24i930234","url":null,"abstract":"Sophorolipid have been identified to possess varying antagonism relationship to a number microbiota, although having been identified as being ecologically friendly. The biosafety of biologically synthesized materials has been identified as a major challenge to commercialization and scale-up. Sophorolipid was produced by Candida haemulonis and Saccharomyces cerevisiae in Palm oil mill effluent impacted soil in Emohua, Rivers State. Submerged fermentation was employed in the production of the sophorolipid while the Well-in-agar approach was employed in the antimicrobial susceptibility was conducted using 20%, 40%, 60%, 80% and 100% on both beneficial and pathogenic bacteria and fungi namely Staphylococcus sp, Nitrobacter sp, Klebsiella sp, Bacillus sp while the fungal flora were Aspergillus sp, Penicillium sp, Rhizopus sp, Candida sp, Mucor sp and Saccharomyces sp. Acute toxicity was conducted using Nitrobacter sp, Nitrosomonas sp and Thiobacillus sp. Probit based determination of acute toxicity after a 48hr and 96hr exposure to the test organisms. Antagonistic nature of the sophorolipids showed there was little or no antagonistic activity on the bacterial isolates than the fungal isolates. The sophorolipid formulation synthesized from both isolates were observed to have a zone of inhibition between 10 mm to 24 mm for Nitrosomonas sp. while to Aspergillus sp it had a range between 4.0 mm to 13.0 mm. Acute toxicity of sophorolipid produced by Candida haemulonis against Nitrosomonas sp 0.054 mg/L while to Thiobacillus sp it had an acute toxicity index of 0.107 mg/L. This study identified that the sophorolipid produced by the yeast isolate had the potential of being employed in a number of sectors. This study has pioneered the untapped benefits in the POME-induced flora and how they can be veritable tools in the biotechnology industry.","PeriodicalId":15023,"journal":{"name":"Journal of Advances in Biology & Biotechnology","volume":"11 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-11-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"80865991","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Relationship of Climatic Variables with Abundance of Indoor Mosquito Genera in Six Communities in Ekiti State, Nigeria","authors":"Omojola F. Olorunniyi","doi":"10.9734/jabb/2021/v24i830233","DOIUrl":"https://doi.org/10.9734/jabb/2021/v24i830233","url":null,"abstract":"Understanding the relationship between climate variables and mosquito abundance is an important factor to determine parasite activity levels and disease risk since various mosquito genera are vectors of parasitic diseases. This necessitated the investigation of relationship between climatic variables and mosquito abundance in Ekiti State, Nigeria with reference to six selected communities. Adult mosquitoes were collected indoor for twelve months in these communities using light traps. The abundance of collected mosquitoes was related with climatic variables (rainfall, relative humidity and temperature). One thousand two hundred and seventeen (1217) adult mosquitoes were collected indoor in all the communities. The population of the mosquito genera was significantly higher (P=0.01) in rainy season than dry season. Average rainfall showed a strong relationship (R2= 0.751) with Anopheles abundance but relationship was weak for both Culex (R2= 0.236) and Aedes (R2= 0.042). The relationship of relative humidity and average temperature with abundance of mosquito genera was generally weak. Since the abundance of mosquito genera was higher in all the communities during rainy season than dry season it will be more appropriate to control mosquitoes in the communities during the rainy season.","PeriodicalId":15023,"journal":{"name":"Journal of Advances in Biology & Biotechnology","volume":"6 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-11-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"88301860","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Martial Jean Huges Kouassi, Gisèle Ahou Yah Koua, Brice Evrad Konan Dibi, M. A. Kouakou, Catherine Bomoh Ebah Djedji, Sidoine Brice Essis, B. N’zué
{"title":"Consumer Preferences for Boiled and Fried Sweet Potato in Central and Northern Côte d'Ivoire, West Africa","authors":"Martial Jean Huges Kouassi, Gisèle Ahou Yah Koua, Brice Evrad Konan Dibi, M. A. Kouakou, Catherine Bomoh Ebah Djedji, Sidoine Brice Essis, B. N’zué","doi":"10.9734/jabb/2021/v24i830231","DOIUrl":"https://doi.org/10.9734/jabb/2021/v24i830231","url":null,"abstract":"Aims: This study evaluated the sensory properties and consumer acceptability of orange-fleshed sweet potato and local sweet potato among households of Central and Northern Côte d’Ivoire. \u0000Study Design: Selection of sweet potato cultivars, determination of nutritional properties, cooking process, and evaluation of hedonic testing and consumer acceptability. \u0000Place and Duration of Study: Bouake and Korhogo district in Central and Northern Côte d’Ivoire, for three years 2018, 2019, and 2020 (July to November). \u0000Methodology: Sensory evaluation and acceptability were performed using a nine-point hedonic scale. The relationships between the sensory attributes and the sweet potato cultivars were analyzed using a Principal Component Analysis plot. Biochemical standard methods were used to determine the dry matter, sugar, total carotenoid contents, and mineral composition of sweet potato cultivars tested by the sensory panel. \u0000Results: All twelve sweet potato cultivars were accepted based on sensory attributes with the different traits of preference. In Bouake district, white (Sanfo Figui 1 and Sanfo Figui 2) and yellow cultivars (Fatoni 2) were most preferred for their texture and yam-like taste, while in Korhogo locality, OFSP (Covington TIB-440060, CIP-199062-1 and Irene) and yellow cultivar (Gotchan) were most accepted because of their attractive appearance and their sweet taste. The OFSP cultivars recorded low dry matter and high sugar content compared to white and yellow varieties. Also, OFSP showed the highest content of carotenoid (181.70 to 351.47 µg/g dw), while local variety recorded low content. All the sweet potato cultivars tested contain mineral components. \u0000Conclusion: This study shows that the local and OFSP varieties were successfully accepted by the consumer with the different quality traits. The main quality traits that determine consumer preference are appearance, texture, and taste (none or sweet taste). Understanding consumer quality traits can increase the effectiveness of breeding programmes, increase yield and adoption of new varieties.","PeriodicalId":15023,"journal":{"name":"Journal of Advances in Biology & Biotechnology","volume":"60 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-11-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"77018603","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
S. D. Ramteke, A. Gavali, S. M. Khalate, A. Langote
{"title":"Evaluation of Bio - Efficacy and Phytotoxicity of Glyphosate 41% SL against Weed Flora in Grape Vineyards","authors":"S. D. Ramteke, A. Gavali, S. M. Khalate, A. Langote","doi":"10.9734/jabb/2021/v24i830230","DOIUrl":"https://doi.org/10.9734/jabb/2021/v24i830230","url":null,"abstract":"The study was evaluated for the bio- efficacy and phytotoxicity of Glyphosate 41% SL against the complex weed flora in grapevines. The experiment was carried out at ICAR- NRC Grapes, Pune with seven treatments replicated thrice. The treatments were imposed at 3-4 leaf stage of weeds in vineyards. All the weed population were niformly distributed in vineyards. All the treatments significantly reduced weed density over untreated control. The highest weed density was recorded with untreated control while, the negligible weed density was recorded in hand weeding followed by Glyphosate 41 % SL @ 4000 ml/ha at 15th, 30th, 45th Days after application. Least dry weight (g/m2) as recorded with Glyphosate 41 % SL @ 4000 ml/ha followed by Glyphosate 41 % SL @ 3000 ml/ha whereas, the maximum dry weight of weed (g/m2) was recorded with untreated control at 45th Days after application. Highest weed control efficacy (%) was recorded with Glyphosate 41 % SL @ 4000 ml/ha followed by Glyphosate 41 % SL @ 3000 ml/ha whereas, negligible weed control efficacy (%) Was recorded with untreated control. The highest yield (kg/vines) was recorded with hand weeding treatments followed by Glyphosate 41 % SL @ 4000. The highest benefit: cost ratio was recorded in the treatment Glyphosate 41 % SL (4000 ml/ha) (1:1.99), while least in control treatment (1:1.77). Applications of Glyphosate 41 % SL @ 4000 ml/ha in grapevines showed highest weed control efficacy (%) and yield per vine (kg/vine) in this investigation.","PeriodicalId":15023,"journal":{"name":"Journal of Advances in Biology & Biotechnology","volume":"27 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-10-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"83537154","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Screening of Agricultural Wastes for Substrates in Oxalic Acid Production Using Aspergillus niger","authors":"Dennis M. Chioma, G. Abu, O. Agwa","doi":"10.9734/jabb/2021/v24i830229","DOIUrl":"https://doi.org/10.9734/jabb/2021/v24i830229","url":null,"abstract":"The disposal and attendant problems associated with agro-wastes have remained a challenge to the environment. Three agricultural wastes (cassava whey, banana peels and groundnut shells) were collected from the Choba and Yam zone markets in Rivers State, Nigeria and screened for their potential as substrates in the formulation of fermentation media to produce oxalic acid. The inoculum for the study was isolated from the banana peels and identified using the megablast search for highly similar sequences from the NCBI non-redundant nucleotide database. The microbial load and proximate composition of the substrates were determined, and the fermentation media formulated. The organism used for the study was identified as Aspergillus niger MW188538. The results showed a total bacterial count of 9.5x104 cfu/ml, 1.87 x 105 cfu/ml, and 4.0 x 104 cfu/g for cassava whey, banana peels and groundnut shell respectively. The carbohydrates of the cassava whey, banana peels and groundnut shells were 67.74 %w/v, 53.24%w/v and 38.8% w/v respectively. After 12 days of fermentation, the substrates from cassava whey, banana peels, groundnut shells accumulated 2.5 ppm, 1.8 ppm and 1.3 ppm of oxalic acid respectively. The study hypothetically indicates that agro-wastes could be utilized as media components for production of industrial organics.","PeriodicalId":15023,"journal":{"name":"Journal of Advances in Biology & Biotechnology","volume":"27 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-10-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"75062099","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}