Journal of Advances in Biology & Biotechnology最新文献

{"title":"Growth Response, Carcass Yield and Meat Quality of West African Dwarf Sheep Fed Graded Level of Shea Cake Meal","authors":"D. Okunlola, A. Amuda, M. Shittu, O. Olatunji, O. T. Ojoawo, O. S. Olaniyan, B. Olateju, A. Fasola, A. Alao","doi":"10.9734/jabb/2021/v24i730237","DOIUrl":"https://doi.org/10.9734/jabb/2021/v24i730237","url":null,"abstract":"Growth response, carcass characteristics and meat quality of West African Dwarf (WAD) sheep fed Shea Cake Meal (SCM) supplement was investigated. A total of 24 WAD sheep of average body weight ranges from 11-12.65 kg comprising of twelve (12) males and twelve (12) females were randomly allotted to four dietary treatments with six animals per treatment in a completely randomized design in a twelve (12) weeks experimental period. Treatment 1 was control and had no inclusion of SCM. Treatments 2, 3 and 4 had 10%, 15% and 20% inclusion of SCM. Results were significantly different (P<0.05) across treatments. Average weight gain ranged from 10.0 kg (SCM-10) to 13.12 kg (SCM-15). T3 recorded highest values for average feed intake (AFI) at a record value of 63.50 kg. T2 had the least feed conversion ratio of 4.81. The carcass quality and sensory evaluation were significantly affected (P<0.05) across the treatments. Hot carcass weight (HCW) ranged from 18.90 kg (SCM-10) to 20.85 kg (SCM-15). Cold carcass weight was recorded 18.05 kg (SCM-10) and 19.95 kg (SCM-20), respectively. Minimum chilling loss of 3.96% was recorded at SCM-15 inclusion level. Animals on SCM-15 inclusion recorded the highest value of 7.55, 7.25 and 7.25 respectively for appearance, tenderness and overall acceptability. SCM-20 had highest value of 7.50 for juiciness compared to SCM-10 and SCM-15 with recorded values of 6.80 and 7.20, respectively. The study showed that all the dietary inclusion was satisfactory as feed for small ruminants without any deleterious effect on their performance as well as on carcass yield and meat quality. Inclusion level of SCM-15 supplement had highest overall performance when fed to (WAD) sheep.","PeriodicalId":15023,"journal":{"name":"Journal of Advances in Biology & Biotechnology","volume":"1 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-09-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"89666595","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Blood Profile and Selected Organ Histopathology of Balami Sheep Fed Shea Cake (Vitellaria paradoxa) Meal","authors":"D. Okunlola, M. Shittu, O. Olatunji, A. Amuda, B. Olateju, A. Fasola, A. Alao","doi":"10.9734/jabb/2021/v24i730232","DOIUrl":"https://doi.org/10.9734/jabb/2021/v24i730232","url":null,"abstract":"The blood profile and histology of selected tissues (kidney, lungs, intestine, and epididymis) of Balami Sheep fed Shea Cake meal supplement was investigated. A total of thirty-two (32) sheep of the average weight range of 11.75-14.75kg comprising an equal number (16) of males and females were randomly allotted to four dietary treatments in a completely randomized experimental design with eight sheep per treatment for twelve weeks duration. Treatment 1 (T1) was a control diet and had zero inclusion level of Shea cake meal (SCM), while treatments 2,3, and 4 had 10%,15%, and 20%  inclusion of SCM, respectively. At the twelfth week of the experiment, two blood samples of 5ml each were taken from each animal from the jugular vein. The first set was preserved in Ethylene Diamine Tetra Acetic Acid (EDTA) bottle for haematological parameters, while the second samples were preserved without EDTA for serum examination. Representative samples of tissues were randomly selected and slaughtered for gross microscopic examination. There was a significant difference (P<0.05) at various levels of inclusion for parameters investigated. The white blood cell (WBC), Red blood cell (RBC), and Packed cell volume (PCV) recorded the highest values at T3 (15% inclusion level) (13.42 x109/L), 2.92x1012/L, 3.75%, respectively). The serum parameters were significantly(p <0.05)different among the treatments. Total protein ranges from 54.78(mg/dl) (T1) - 60.51(mg/dl) (T2), Cholesterol70.15(mg/dl) (T1) - 78.00(mg/dl) (T4). Iron ranges from 58.15(ug/dl) (T1) - 62.88(ug/dl) (T3).The histopathology of tissues (kidney, lungs, intestine, and epididymis) showed no alteration across the treatments (P= .05) in the gross microscopic examinations. No lesion was observed in the lungs, kidney, epididymis, and intestine. The results showed that shea cake meal posed no health risk to Balami sheep and as such recommended as a good supplement in the diets of the sheep.","PeriodicalId":15023,"journal":{"name":"Journal of Advances in Biology & Biotechnology","volume":"8 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-09-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"75239318","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A Mathematical Graph-Theoretic Model of Single Point Mutations Associated with Sickle Cell Anemia Disease","authors":"Edem K. Netsey, Samuel Kakraba, S. Naandam, Aayire C. Yadem","doi":"10.24297/jbt.v9i.9109","DOIUrl":"https://doi.org/10.24297/jbt.v9i.9109","url":null,"abstract":"Many diseases like cystic fibrosis and sickle cell anemia disease (SCD), among others, arise from single point mutations in the respective proteins. How a single point mutation might lead to a global devastating consequence on a protein remains an intellectual mystery. SCD is a genetic blood-related disorder resulting from mutations in the beta chain of the human hemoglobin protein (simply, β-globin), subsequently affecting the entire human body. Higher mortality and morbidity rates have been reported for patients with SCD, especially in sub-Saharan Africa. Clinical management of SCD often requires specialized interdisciplinary clinicians. SCD presents a major global burden, hence an improved understanding of how single point mutations in β-globin results in different phenotypes of SCD might offer insight into protein engineering, with potential therapeutic intervention in view. By use of mathematical modeling, we built a hierarchical (nested) graph-theoretic model for the β-globin. Subsequently, we quantified the network of interacting amino acid residues, representing them as molecular system of three distinct stages (levels) of interactions. Using our nested graph model, we studied the effect of virtual single point mutations in β-globin that results in varying phenotypes of SCD, visualized by unsupervised machine learning algorithm, the dendrogram.","PeriodicalId":15023,"journal":{"name":"Journal of Advances in Biology & Biotechnology","volume":"1 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-09-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"84405117","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Phenological Study of Irvingia gabonensis (Aubry-Lecomte ex O’ Rorke) Growing in Ihiala, Anambra State, South-East, Nigeria","authors":"Ochia M. Angela, Ekwealor U. Kenneth, Okigbo N. Raphael, I. F. Chisom","doi":"10.9734/jabb/2021/v24i730225","DOIUrl":"https://doi.org/10.9734/jabb/2021/v24i730225","url":null,"abstract":"Phenology refers to periodic biological phenomena that are correlated with climatic conditions. Very little is known about the phenological pattern of most tropical fruit trees especially in the South-East, Nigeria. The aim of this study was to provide a phenological data and baseline information on the phenology of Irvingia gabonensis (Aubry-lecomte ex O’Rorke). The study was conducted with exotic species at Ihiala, South-East, Nigeria, a tropical climate. The initiation and completion of different phenophases of the tree, such as budding, leafing, flowering, fruiting and ripening were recorded at 2 weeks interval. The combination of the principal growth stages and the secondary growth stages produced 2-digit codes, were used to delineate the time-dependent phenophases. The results showed that changes in the prevailing seasons influenced the vegetative phenophases in I. gabonensis. There are splashes of rains even in the supposed dry months. Hence, bud formation, leafing and leaf fall phenophases were overlapping all through the seasons. Flowering phenophase peaked by March/April with the coming of the rains. Also fruiting and ripening peaked by June/July respectively (rainy season). Thus the study has revealed that the major seasons in the area (rainy and dry seasons) influence the various phenophases in I. gabonensis. The study has equally revealed the phenological  data and base line information on the phenology of I. gabonensis which will in the future serve as correlation between phenology and climate change in the area. The study has revealed that the phenology of I. gabonensis may not be stable or resilient since it is influenced by these changing seasons, little wonder then a shift in phenology is recorded in this study.","PeriodicalId":15023,"journal":{"name":"Journal of Advances in Biology & Biotechnology","volume":"13 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-09-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"89380536","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effect of Guiera senegalensis and Natron on Serum Indices of Cardiac Function of Postpartum Rats","authors":"A. Abubakar, Jabir Aliyu Danyaya, K. Abubakar, Magaji Umar Faruk, Y. Saidu","doi":"10.9734/jabb/2021/v24i730226","DOIUrl":"https://doi.org/10.9734/jabb/2021/v24i730226","url":null,"abstract":"Peripartum cardiomyopathy (PPCM) is a rare but devastating cardiac failure of indeterminate etiology occurring in late pregnancy or early puerperium. The prevalence of PPCM in Northern Nigeria could be attributed to the unique customary puerperal practices of prolonged consumption oflarge quantities of Guiera senegalensis and Natron. This study investigated the effect of decoction of G. senegalensis with and without Natron on cardiac markers, lipid profile and histology ofheart in postpartum female albino rats using standard methods.Thirty-five (35)female albino rats of body weights 190-200 g of five month were randomly grouped into seven (7) of five rats each. Group 2, 3 and 4 were orally administered with 100, 200 and 300 mg/kg body weight of G. senegalensis. Group 5, 6 and 7 were orally administered with 100, 200 and 300 mg/kg body weight (1:1) of G. senegalensis and Natron by gavage, whereas group 1 received distilled water. The study revealed significant (p< 0.05) decrease in triglyceride(TG) in group 2 to VII, high density lipoproteincholesterol (HDL-c) in group 2, 5 and 6, very low density lipoprotein- cholesterol (VLDL-c) in group 3 to V, couples with significant (p< 0.05) increase in low density lipoprotein cholesterol (LDL-c) in group 2, 3, 5 and 7, and for (AIX) in group 2, total cholesterol(TC) in group 2, 3 and 7 when compared with control. Significant (p< 0.05) increase in Lactate dehydrogenase (LDH) in group 3 to 7, and Myoglobin (MB) in group 3 was observed. Significant (p< 0.05) decrease in Troponin I (TnI) in group 2 and 4 was seen. There were no significant (p>0.05) changes in Troponin T (Tn T), aspartate amino transferase (AST) and Creatine Kinase(CK-NAC) of the treatment group when compared to control group. Histopathological examination of section of the heart revealed ventricular dilatation, hypertrophied with enlarged nuclei, stretched, and irregular and mural thrombi in group 2 to 4 and 7. Inflammation in group 5 and 7, Pigment deposited in group 4 to 7 and Mild fibrosis in group 3 were observed. The study demonstrates that consumption of G. senegalensis and Natron for 28 days’induces dyslipidemia and causes changes in the heart of postpartum rats and thus may contribute to the pathogenesis of PPCM.","PeriodicalId":15023,"journal":{"name":"Journal of Advances in Biology & Biotechnology","volume":"31 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-09-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"73552189","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Bacteriological Assessment of Automated Teller and Point of Sales Machine User Interface in Uyo Metropolis, Nigeria","authors":"O. John, Christiana E. Asuquo, M. M. Akpan","doi":"10.9734/jabb/2021/v24i630220","DOIUrl":"https://doi.org/10.9734/jabb/2021/v24i630220","url":null,"abstract":"The Automated Teller Machine (ATM) and Point of Sales (POS) machine user interface of different banks, schools, hospitals, restaurants/eateries, shopping malls and petrol/gas stations in Uyo metropolis were bacteriologically assessed in the dry and wet seasons using cultural technique. The result of fomites key pads indicate total heterotrophic bacteria counts (THBC) was in the order: Banks > Hospitals > Schools > Petrol/Gas stations > shopping malls > Restaurants/Eateries in both seasons. There was an increase in the bacterial counts of the fomites from all locations in the wet season compared to the dry season and the difference significant (p = 0.05). The THBC from ATMs in banks, hospitals and schools ranged from 5.6 ± 0.1 to 7.8 ± 0.6 Log₁₀CFU/cm2 and from POS 3.4 ± 0.3 to 5.0 ± 0.2 Log₁₀CFU/cm2) for Petrol/Gas stations, shopping malls and Restaurants/Eateries in the wet season. The dry season revealed THBC range of 4.1± 0.6 to 6.4 ± 0.3 Log₁₀CFU/cm2 for ATMs and 2.6 ± 0.8 to 3.8 ± 0.1 Log₁₀CFU/cm2 for POS. Organisms associated with the fomites were Escherichia coli, Staphylococcus aureus, Staphylococcus epidermidis and species of Bacillus, Pseudomonas, Proteus, Streptococcus, Salmonella, Shigella, Micrococcus, Vibrio and Klebsiella. The highest and least frequency of occurrence in the dry season indicated Micrococcus (17.2%) and Vibrio (1.4%) in relation to Staphylococcus aureus (18.2%) and Proteus sp. (2.8%) in the wet season. The Gram-positive organisms exhibited high and low susceptibility to Gentamycin and Augmentin compared to Ofloxacin and Amoxicillin respectively in Gram-negative bacteria. The results indicate ATM and POS machine user interface as possible sources of pathogenic organisms. Therefore, regular cleaning of ATM and POS user interface and public awareness on the need for adequate personal hygienic practice after the use of these machines is recommended to reduce associated risks.","PeriodicalId":15023,"journal":{"name":"Journal of Advances in Biology & Biotechnology","volume":"18 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-07-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"73897762","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effects of Consuming Different Varieties of Bambara Nut (Vigna subterranea) Seeds on Liver and Kidney of Diabetic and Non-diabetic Subject","authors":"A. Mohammed, D. Mhya","doi":"10.9734/JABB/2021/V24I630219","DOIUrl":"https://doi.org/10.9734/JABB/2021/V24I630219","url":null,"abstract":"Aims: Bambara nut seeds have been reported as a good source of food with high fiber and nutritional contents, and is consumed by persons with diabetes as one of the dietary therapy. However, the plant seeds are of different varieties and whether they might have vary biological effects on renal and liver functions is not fully known. This has prompted the study to evaluate effects of consuming different varieties of Bambara Nut seeds on liver and kidney of both diabetic and non-diabetic rats. \u0000Methodology: Four Bambara nut seeds varieties were dehulled and each heated at 60oC, cooled then grounded into flour using a mechanical grinder. The flours were used to formulate feeds which were given to different diabetic and non-diabetic rats’ groups as follows; group A (ALK01 feed), group B (ALK02 feed), group C (ALK03 feed), group D (ALK04 feed), group E (Basal feed), and group F (Normal animal’s feed). The animals were fed for 28 days then sacrifice, blood collected and serum separated and used for hepatic and renal parameters analysis. \u0000Results: The results of the study showed decrease levels of serum total protein and albumin, and elevation of AST and ALT activities as well as urea and creatinine concentrations in diabetic rats fed basal and normal diets, whereas, reverse in these parameters were recorded from diabetic rats fed varieties of Bambara nut seeds’ formulated feeds.  \u0000Conclusion: Findings from the study suggest consumption of Bambara nut seeds of different varieties is safe and has ability to ameliorate diabetic hepatic and renal failure in a variety dependent manner where; ALK 04 is more likely the most effective.","PeriodicalId":15023,"journal":{"name":"Journal of Advances in Biology & Biotechnology","volume":"22 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-07-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"78314458","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Seedling Growth of Tectona grandis using Different Potting Mixture","authors":"G. Omokhua, C. Fredrick, C. N. Okakpu","doi":"10.9734/JABB/2021/V24I630218","DOIUrl":"https://doi.org/10.9734/JABB/2021/V24I630218","url":null,"abstract":"Aims: This study evaluated the influence of 11 potting media compositions on seedling growth and development of Tectona grandis. \u0000Study Design: The experiment was set up in a completely randomized design involving ten replicates. \u0000Place and Duration of Study: The study was carried out at the Department of Forestry and Wildlife Management Nursery, University of Port Harcourt, Rivers State, Nigeria, between April 2019 and April 2020. \u0000Methodology: Ten (10) seedlings per sowing media including topsoil, topsoil and cow dung (2:1, 3:1), topsoil and poultry dung (2:1, 3:1), topsoil and pig dung (2.1, 3:1), topsoil and sawdust (2.1, 3:1), topsoil and mushroom substrate (2.1, 3:1) were selected and transplanted into polybags. Growth parameters (seedling height, root collar diameter, leaf number and biomass (shoot dry weight (SDW), root dry weight (RDW) and total dry weight (TDW)) were determined and subjected to analysis of variance. \u0000Results: Seedlings of T. grandis displayed significant differences (p ≤ 0.05) in height, root collar diameter, leaf number and biomass. Among treatments, tallest seedlings were observed in mixture of topsoil and poultry dung (3:1), at months 1 to 3 and in mixture of topsoil and cow dung (3:1) at months 4 and 5, highest root collar diameter was observed in mixture of topsoil and poultry dung (3:1) and highest leaf number was recorded in mixture of topsoil and poultry dung (2:1 and 3:1) and topsoil and cow dung (2:1 and 3:1) at months 1 to 5. The highest SDW was evident in mixture of topsoil and poultry dung (2:1) while highest RDW and TDW were observed in mixture of topsoil and cow dung (3:1). Lowest height, diameter, leaf number and biomass were recorded in topsoil (control) at months 1 to 5. \u0000Conclusion: The results revealed that among the tested mixtures, topsoil and poultry dung and topsoil and cow dung mixtures were the best for seedlings growth of T. grandis.","PeriodicalId":15023,"journal":{"name":"Journal of Advances in Biology & Biotechnology","volume":"62 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-07-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"84905542","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The Antimicrobial, Anti Inflammatory and Analgesic Activities of the Rhizome Extract of Curcuma longa L. (Turmeric)","authors":"Ogbonna Abigail Ify, Adejube Glory Raphael, Okoye Clifford Tochukwu, Ogbonna Ugoy Sonia Amarachi, N. Ikechukwu, Madu Josephine Madukaihe, Yakubu Thomas, Ogbonna Chike Innocent","doi":"10.9734/JABB/2021/V24I630217","DOIUrl":"https://doi.org/10.9734/JABB/2021/V24I630217","url":null,"abstract":"Aim: This study aimed at investigating the anti-microbial, anti-inflammatory, and analgesic properties of rhizome extracts of Curcuma longa (Turmeric). \u0000Methodology: Extraction of biochemical constituents of the 150 g of the turmeric rhizomes powder was carried out successively using hexane, ethyl acetate, methanol, and water as solvents. The antimicrobial potentials of the different fractions of the extract were tested on clinical bacterial including Staphylococcus aureus, Streptococcus pneumonia, Klebsiella pneumonia and Pseudomonas aeruginosa using agar well diffusion method at concentrations of 400 mg/ml, 200 mg/ml, 100 mg/ml, 50 mg/ml, and 25 mg/ml. The Minimum Inhibitory Concentration (MIC) and Minimum Bactericidal Concentration (MBC) were determined using agar dilution technique. For the anti-inflammatory effects of the extract of C. longa, albino rats were treated with methanolic extract (250, 500, and 1000 mg/kg body weight) and diclofenac as control, after which they were injected with egg albumin on the right hind paw to induce inflammation. The swelling degree of the injected paw was measured. The analgesic effect of C. longa methanolic extract on hot plate-induced pain was also investigated in albino rats, and the Index of response latency was recorded. \u0000Results: The biochemical assay showed that C. longa extract contained Alkaloids, Saponins, Tannins, Flavonoids, Carbohydrate, Steroids, Terpenes, Anthraquinone, and Cardiac Glycosides, with Methanol and aqueous extracts having the most biochemical constituents. The antibacterial susceptibility screening showed that K.pneumonia was the most susceptible organism, having the highest zone of inhibition of 18 mm for ethyl acetate extract at the concentration of 400 mg/ml. This was followed by S. pneumonia, S.aureus and P. aeruginosa with inhibition zone of 13, 12 and 10 mm respectively. The MIC for K.pneumonia and S.aureus were both 200 mg/ml, while that of P. aeruginosa and S. pneumonia were both 400 mg/ml. The paw size of rats treated with increasing doses of the extract and diclofenac significantly decreased with time. The concentration of 250 mg/kg had the lowest paw volume of 4.39±0.36mm while 1000 mg/kg recorded 5.18±0.28 as the highest, at 180 minute. \u0000Conclusion: Based on the result obtained, it can be concluded that Curcuma longa Rhizome extract possess anti-microbial, anti-inflammatory, and analgesic properties.","PeriodicalId":15023,"journal":{"name":"Journal of Advances in Biology & Biotechnology","volume":"52 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-07-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"79857243","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effect of Hormonal Regime and Explant Type on Cell Clusters Expression of Maize Mutants (Zea Mays L) Derived from Gamma Irradiated Seeds of Ev8728 variety","authors":"K. Nadia, A. Koutoua, Y. Konan, Kassi Amalan Angèle, Soro Dogniméton, Kouakou Tano Hilaire, K. Justin","doi":"10.9734/jabb/2021/v24i530213","DOIUrl":"https://doi.org/10.9734/jabb/2021/v24i530213","url":null,"abstract":"Maize (Zea mays L.) seeds irradiated with gamma or not from the fourth self-fertilisation cycle were selected for tissue culture. For this purpose, MS medium supplemented with 30 g.L-1 sucrose, 100 mg.L-1 casein hydrolysate, 100 mg.L-1 myo-inositol and 6 g.L-1 agar was used. For this purpose, three auxins type (2.4-dichlorophenoxyacetic acid (2.4-D), 2-methoxy-3.6-dichlorobenzoic acid (Dicamba) and Indole-3-Acetic Acid (AIA)) and explants (root, epicotyl and leaf) were tested. The results showed that 2.4-D more precisely at 3.5 mg.L-1 was the best auxin for callus induction in the different maize mutants studied. The induction rate, dry matter weight and water content of callus varied according to the type, age, explant position and the maize mutants studied (control, 200 and 300 grays). Thus, the 7-day root and more precisely its basal part was better for callogenesis. In addition, mutants of the 200 grays dose were more expressive in the ability to induce callus in EV8728maize variety.","PeriodicalId":15023,"journal":{"name":"Journal of Advances in Biology & Biotechnology","volume":"105 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-06-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"85901767","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}