Journal of Advances in Biology & Biotechnology最新文献

{"title":"A Comparative Review of the Traditional and Modern Methods of Water Treatment","authors":"A. O. Adeniyi","doi":"10.9734/JABB/2020/V23I1130191","DOIUrl":"https://doi.org/10.9734/JABB/2020/V23I1130191","url":null,"abstract":"Artificial pollution of groundwater may arise from either point or diffuse sources. Several different means to improve the microbial quality of water and reduce waterborne diseases have been documented around the world. Traditional methods of water treatment include Filtration through winnowing sieve (used widely in Mali), Filtration through cloth (commonly used in villages in India, Mali and the southern part of Niger), Filtration through clay vessels (used in Egypt), filtration through plant material (commonly used in Tamil Nadu and Kerala, India) and Jempeng stone filter method (used in Bali, Indonesia). Modern methods of water treatment include Solar Water Disinfection (SODIS), LifeStraw, Nanofilter, Ceramic water filter, Bio-sand filter and Kanchan Arsenic filter. Comparatively, traditional methods of water treatment employ crude methods which have proved to be effective in relation to filtration but the modern methods make use of newer research technologies in the elimination of pathogens and toxic chemicals to make drinking water more potable and safe.","PeriodicalId":15023,"journal":{"name":"Journal of Advances in Biology & Biotechnology","volume":"45 1","pages":"44-51"},"PeriodicalIF":0.0,"publicationDate":"2020-12-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"73898965","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Efficacy of Mancozeb and Ocimum gratissimum Extract in the Management of Late Blight Disease in Tomato Varieties","authors":"M. Bi, Ache NehTeke, Nkwatoh Therese Ncheuveu, Bih Nadine Toh","doi":"10.9734/JABB/2020/V23I1030183","DOIUrl":"https://doi.org/10.9734/JABB/2020/V23I1030183","url":null,"abstract":"Tomato late blight is an economic disease that causes 100 % yield loss of tomato in Cameroon. The objective of the study was to determine the efficacy of mancozeb and Ocimum gratissimum extract in managing late blight disease in Nadira and Rio-grande tomato varieties. Seedlings of each tomato variety were planted in replicates of three in a complete randomized block design comprising of three treatments (T1 –Ocimum gratissimum extract (1666.7 g/15 L); T2 –Mancozeb (50 g/15 L); T3 –control. Fifty grams of mancozeb (50g) and 1666.7 g of O. gratissimum extract dissolved and mixed with water in a 15 L knapsack sprayer was applied to control tomato late blight from the onset of blight symptoms, at two days intervals, to control Data for the disease incidence, severity, and yield parameters were collected at weekly intervals for four weeks from plants in the middle of the ridges. Mancozeb and O. gratissimum extract significantly reduced the disease Original Research Article Bi et al.; JABB, 23(10): 45-53, 2020; Article no.JABB.65085 46 severity of Nadira and Rio-grande tomato varieties by 0.2., resulting in a mean fruit number of 18.31 and 16.31 fruits and fruit weight of 1.44 g and 1.13 g. There was no significant difference (p =0.05) between plants sprayed with Mancozeb and O. gratissimum extract. The Nadira variety was resistant to the tomato late blight caused by Phytophthora infestans. Thus biopesticides (O. gratissimum) can be effectively used as alternatives to synthetic fungicides (mancozeb) –which pose risks to human and environmental health.","PeriodicalId":15023,"journal":{"name":"Journal of Advances in Biology & Biotechnology","volume":"86 1","pages":"45-53"},"PeriodicalIF":0.0,"publicationDate":"2020-12-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"85579089","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Molecular Diversity Analysis in Cotton (Gossypium hirsutum L.) Using RAPD Markers","authors":"M. A. Wahab, S. Sumi, A. Islam, M. E. Houqe","doi":"10.9734/JABB/2020/V23I1030182","DOIUrl":"https://doi.org/10.9734/JABB/2020/V23I1030182","url":null,"abstract":"Cotton (Gossypium hirsutum L) is considered as major textile fiber around the world. This research aimed to analyze the molecular diversity among 9 cotton genotypes collected from Cotton Development Board, Bangladesh using the 7 Random Amplified Polymorphic DNA (RAPD) markers. Some total of 18 DNA bands were obtained and among them, 13 were polymorphic bands. The range of DNA amplification varied from 180 to 800 bp. The percentage of polymorphism was about 50.71. Genetic diversity ranged from 0.22 to 0.44 with an average value of 0.34. Nei’s genetic distance ranging from 0.1667 to 0.6667 and most importantly PIC value ranged from 0.18 to 0.35 with an average value of 0.27. The PIC value indicated that most of the studied cotton genotypes were moderately diversified and homogenous as well as no heterozygosity found. A dendrogram indicating the relative genetic similarity of the Bangladeshi cotton genotypes was constructed which followed in two major clusters (A and B) among the studied material. The Cotton Development Board (CDB), Bangladesh committed to working on cotton improvement and this investigation will be helpful for the selection of diversified genotypes in varietal improvement in cotton. The output of this research will be a baseline for future molecular research work on cotton genotypes in Bangladesh.","PeriodicalId":15023,"journal":{"name":"Journal of Advances in Biology & Biotechnology","volume":"50 1","pages":"33-44"},"PeriodicalIF":0.0,"publicationDate":"2020-12-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"86106244","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Morphological and Molecular Identification of Mycobiota Associated with Juniperus monosperma (Engelm.) Sarg. Blight in Mexico","authors":"Ma. Elizabeth Galindo-Cepeda, Abiel Sánchez-Arizpe, E. Angel, S. F. Velázquez-Morales, J. L. Arispe-Vázquez","doi":"10.9734/JABB/2020/V23I1030180","DOIUrl":"https://doi.org/10.9734/JABB/2020/V23I1030180","url":null,"abstract":"The aim of the investigation was to identify the agents associated with blight in Juniperus monosperma at Lirios region, Arteaga, Coahuila. Botanical material was collected at Lirios, Arteaga, and taken to the laboratory. Pathogens were isolated in ADP culture medium and identifying by morphological criteria and molecular using primers ITS1 and ITS4. DNA extraction by the Dellaporta method, the visualization of obtained DNA was performed by electrophoresis on a 2% (p/v) agarose gel. DNA quantification was performed on a NanoDrop 1000 spectrophotometer, and DNA amplification was carried out in the Veriti thermocycler. Obtained sequences were aligned and compared with those available in the GenBank database of the National Center for Biotechnology Information (NCBI), using the BLAST algorithm (Basic Local Aligment Search Tool) to find conserved sequences. Pathogens were inoculated in stems of J. monosperma. The agents associated with blight were Alternaria sp., Aspergillus sp. and Rosellinia sp. and the sequence obtained compared with BLAST coincided only with Alternaria sp. and Aspergillus sp. with the Original Research Article Galindo-Cepeda et al.; JABB, 23(10): 1-7, 2020; Article no.JABB.63730 2 access codes KP027305.1 and MG551283.1, respectively. According to data taken in field, Alternaria sp. behaved as the highest prevalence species and severity associated with blight in J. monosperma, and Rosellinia sp. in laboratory conditions.","PeriodicalId":15023,"journal":{"name":"Journal of Advances in Biology & Biotechnology","volume":"128 1","pages":"1-7"},"PeriodicalIF":0.0,"publicationDate":"2020-12-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"88009412","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Probiotics as Agents of Health Improvement, Infection Control and Diseases Treatment: A Review","authors":"Francis A. Gbadamosi, I. R. Akaniro, M. Olaitan, Theresa O. Nnamocha","doi":"10.9734/JABB/2020/V23I930179","DOIUrl":"https://doi.org/10.9734/JABB/2020/V23I930179","url":null,"abstract":"Aims: This review presents valuable information on the contribution of probiotics to the improvement of health and quality of life. Background: Due to their roles in normal physiology and impacts on human health, probiotics have become a subject of great interests especially in the field of Microbiology. The results of several well-designed clinical studies have shown probiotics to be reliable therapeutic option for the prevention, treatment and control of various health conditions; gastrointestinal diseases, respiratory diseases, neurological disorders, depressive symptoms and autoimmune disorders. Probiotics enhance gastrointestinal tract (GIT) health via vitamins production and supply to the human host and have also been reported to improve immune function thus, inhibiting pathogen invasion and colonization/development. Some show direct/indirect immune modulating capabilities by enhancing mucosal barrier functions, modulating sensory motor functions and delivering antigens. Many Review Article Gbadamosi et al.; JABB, 23(9): 37-48, 2020; Article no.JABB.64397 38 probiotic foods possess antioxidant properties that enable them to withstand oxidative stress and strengthen hosts’ anti oxidative defense mechanism. Findings/Conclusion: The potential application of probiotics in functional foods and pharmaceuticals could demonstrate the improvement of health and quality of life.","PeriodicalId":15023,"journal":{"name":"Journal of Advances in Biology & Biotechnology","volume":"76 1","pages":"37-48"},"PeriodicalIF":0.0,"publicationDate":"2020-12-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"76213851","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Microbiology of Sandwiches: “Street – Foods” and “Fast – Foods”","authors":"A. Chesca, Karina Damião Brandão, C. Mangucci","doi":"10.9734/jabb/2020/v23i930178","DOIUrl":"https://doi.org/10.9734/jabb/2020/v23i930178","url":null,"abstract":"Aims: The aim of this study was to investigate the microbiological quality and the presence of pathogens in sandwiches from different snack bars. Study Design: Foods ready for consumption present a composition which facilitates their deterioration and care from production to consumption ensures its quality. The sandwich is a product of high consumption for its easy acquisition and despite its immediate consumption the sandwich can be considered a food offering risk to the consumers because it is composed of different ingredients that require excessive manipulation. Place and Duration of Study: Food Microbiology Laboratory University of Uberaba. Methodology: For this study, 30 samples of this product were collected from the popular snack bars, street-foods and reputable fast-foods chains in Uberaba trade. The samples were transported to the Laboratory of Food Microbiology of the University of Uberaba in isotherm boxes filled with ice where they were kept under refrigeration until the time of analysis. The microbiological analyses were done according to the methods proposed by Vanderzant and Splittstoesser (1999) and Silva et al. (2007). Results: From the total of the samples analyzed, 36.6% presented fecal coliforms and Salmonella Original Research Article Chesca et al.; JABB, 23(9): 31-36, 2020; Article no.JABB.63904 32 sp.; 46.6% presented coagulase-positive Staphylococcus and 30% Bacillus cereus. There was no presence of sulphite-reducing clostridium. Conclusion: The presence of coagulase-positive Staphylococcus aureus indicates the lack of hygiene by food handlers because it is a living microorganism from the nasopharynx and the presence of Salmonella sp. and fecal coliforms indicate poor hygiene and sanitary handling. The improper handling and lack of care in relation to good practices favor the food poisoning.","PeriodicalId":15023,"journal":{"name":"Journal of Advances in Biology & Biotechnology","volume":"294 1","pages":"31-36"},"PeriodicalIF":0.0,"publicationDate":"2020-12-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"85463406","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Research Progress on Auricularia delicata","authors":"Li Xiao, Muharagi Samwel Jacob, Zhang Bo, X. An-ran","doi":"10.9734/JABB/2020/V23I1030181","DOIUrl":"https://doi.org/10.9734/JABB/2020/V23I1030181","url":null,"abstract":"Aims: To describe the current status of A. delicata emphasizing on the key parameters; occurrence, classification, molecular studies, nutritional and medicinal benefits, cultivation status, and future development perspective. Place and Duration of Study: China–Zambia Agricultural demonstration center and Engineering Research Center of Chinese Ministry of Education for Edible and Medicinal Fungi, Jilin Agricultural University, China between July 2019 and June 2020. Methodology: In this study, various literatures were reviewed for each parameter studied. Findings were deduced from current literatures and discussed. Results: The screening of bioactive contents of A. delicata revealed the presence of phenolic compounds; chlorogenic, flavonoids, and ethyl acetate, polysaccharides; Chitosan, fibers, βglucans, mannans, chitin, and melanin. These substances exhibit hepatoprotective effects, antioxidant activities and antimicrobial activities against some microbes like Candida albicans, Bacillus subtilis, Enterococcus faecium, Streptococcus aureus, Bacillus cereus, Salmonella typhi Review Article Xiao et al.; JABB, 23(10): 8-32, 2020; Article no.JABB.64082 9 and Escherichia coli. A. delicata also contains several nutrients namely; protein, vitamin B2, vitamin C, and minerals; Potassium, Calcium, Iron, Magnesium, Zinc, Manganese, that play a vital role in human growth and development. Moreover, its cultivation using various technologies provides an opportunity for high yield production. A. delicata was recently studied at Jilin Agricultural University and domesticated in Heilongjiang province in the northeastern part of China. Due to the similarity of its fruiting body with the structure of the deer tripe, it was assigned a common name as “Deer tripe mushroom”. A. delicata mycelia are capable of growing on several culture media with different nutritional profiles, optimal temperatures and pH values. It is cultivated under tropic temperatures ranging from 25°C-30°C, optimal pH of 6.5, and humidity 80-90%. The commonly used media include; Potato Dextrose Agar (PDA), Yeast Extract Agar (YEA) and Malt Extract Agar (MEA). Conclusion: Therefore the above mentioned significant properties (occurrence, nutritional, medicinal, and cultivation) provide a foundation for further research on the development and utilization of A. delicata.","PeriodicalId":15023,"journal":{"name":"Journal of Advances in Biology & Biotechnology","volume":"281 1","pages":"8-32"},"PeriodicalIF":0.0,"publicationDate":"2020-12-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"77728285","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Free Radical Scavenging and Antioxidant Potential of Ethanolic Leaf Extract of Diodia sarmentosa on High Fat Fed Wistar Rats","authors":"E. T. I. Ndubuisi, Korie Stephen Chinedumije, P. Chukwudi","doi":"10.9734/JABB/2020/V23I1030184","DOIUrl":"https://doi.org/10.9734/JABB/2020/V23I1030184","url":null,"abstract":"Diodia sarmentosa (Sw) commonly known as Zimbabwe flora or Tropical button weed is a straggling or procumbent perennial herb which grows in evergreen forest, riverine vegetation and bush land. It has been used traditionally for the treatment of ulcer, diabetes and other ailments. This study investigated the Free radical scavenging and antioxidant potential of ethanolic leaf extract of Diodia sarmentosa on high fat fed wistar rats. Thirty (30) male wistar rats (150 g-200 g) were divided into five (5) groups: Negative control rats, Positive control rats, Low dose extract rats treated with 250 mg/kg, High dose extract rats treated with 500mg/kg of the extract, and Standard antihyperlipidaemic drug rats treated with 5 mg/kg of Simvastatin. High fat diet was fed to the rats with Ghee and Coconut oil in the ratio of 3:1 for six (6) weeks, and administration of the treatments started from the 3rd week till the 6th week. The free radical scavenging and antioxidant potentials of Diodia sarmentosa was determined by assessing parameters like nitric oxide radical, hydroxyl radical, malondialdehyde (MDA), Superoxide dismutase (SOD), Catalase (CAT), Glutathione peroxidase (GPx) and Glutathione S-transferase (GST) in the serum of the test rats. Diodia Original Research Article Ezejiofor et al.; JABB, 23(10): 54-63, 2020; Article no.JABB.64944 55 sarmentosa scavenged free radicals In vitro by inhibiting Nitric oxide and Hydroxyl radicals in a concentration-dependent manner, showing an inhibitory concentration at 50% (IC50) of 907.17 μg/ml ± 45.36 and 2173.44 μg/ml ± 100.11 respectively. Results obtained showed antioxidant enzymes like SOD, GPx and CAT in the serum of the test rats were significantly increased (p<0.05) by both doses of the extract when compared to the PC group and was similar to the standard drug used. GST activity was decreased in the serum by both doses of the extract, this decrease was not significant (p>0.05), while Malondialdehyde level was significantly decreased (p<0.05) by both doses of the extract in the serum of the tests rats. Diodia sarmentosa has antioxidants potentials because of its ability to scavenge free radicals, replenish antioxidant enzymes and reduce lipid peroxidation.","PeriodicalId":15023,"journal":{"name":"Journal of Advances in Biology & Biotechnology","volume":"70 1","pages":"54-63"},"PeriodicalIF":0.0,"publicationDate":"2020-12-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"84098583","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Production, Partial Purification and Characterization of Two α-Amylase Isoforms from Saccharomyces cerevisiae strain YOP 1/2-2 Isolated from Tchapalo (Côte d’Ivoire)","authors":"Ouattara Lacinan, Koné Fankroma Martial Thierry, Djoudy Alix Evrard, N. K. Florent, Dabonné Soumaïla","doi":"10.9734/jabb/2020/v23i930177","DOIUrl":"https://doi.org/10.9734/jabb/2020/v23i930177","url":null,"abstract":"Amylases play an important role in biotechnology and find applications in several industrial fields such as pharmaceutical, food, paper, cosmetics and detergents. Thus, it appears necessary to identify new sources of amylase, especially from microbial origin, due to the low energy consumption, cost-effective, high metabolic diversity, rapid cell growth, non-toxic and eco-friendly characteristics. In the present report, we carried out the production and partial purification of αamylase by Saccharomyces cerevisiae strains isolated from Tchapalo, a traditional alcoholic beverage of Côte d'Ivoire. Five fungal isolates were screened initially for α-amylase production by using method of wells on Yeast Extract Peptone Dextrose Agar medium, a complete medium for yeast growth. One step DEAE-Sepharose Fast Flow was achieved for partial purification of αOriginal Research Article Lacinan et al.; JABB, 23(9): 17-30, 2020; Article no.JABB.63420 18 amylase obtained. Among yeasts, isolate S. cerevisiae YOP 1/2-2 was able to provoke starch hydrolysis halo of 15.067±0.12 mm on starch agar plate after 48 h of incubation at 30°C. The partial purification of resulting enzyme showed two protein peaks, designated α-amylase 1 (AMY1) and α-amylase 2 (AMY2) with amylolytic activity and specific activities of 1.57-1.58 U/mg protein. Both isoforms (AMY1 and AMY2) were thermostable with optimal activity at 50 and 55°C, respectively, and at pH ranged from 4.5 to 5.3 in 0.1 M sodium acetate buffer. EDTA and Cd strongly inhibited α-amylase activity by 72-75% and 64-65%, respectively, whereas cations Ca 2+ and Mn showed 85-99% and 71% increased amylolytic activity, respectively. All these properties show the potential uses of α-amylases from S. cerevisiae in the industrial transformation of starch.","PeriodicalId":15023,"journal":{"name":"Journal of Advances in Biology & Biotechnology","volume":"30 1","pages":"17-30"},"PeriodicalIF":0.0,"publicationDate":"2020-12-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"88953150","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Evaluation of Vegetable Contamination with Medically Important Helminths and Protozoans in Calabar, Nigeria","authors":"Imalele Edema Enogiomwan, Evbuomwan Owen Ikponmwosa, O. Chinyere, Akpan Blessing Christopher","doi":"10.9734/jabb/2020/v23i930176","DOIUrl":"https://doi.org/10.9734/jabb/2020/v23i930176","url":null,"abstract":"Aims: Vegetables are usually exposed to parasitic ova, larvae or oocyst during cultivation, irrigation, storage, transportation or while processing for consumption. Consumption of raw, unwashed and improperly cooked vegetables is considered a risk factor for transmission of intestinal parasites. This study assessed the parasitic contamination of vegetables and also determined factors associated with parasitic contamination of vegetables in selected markets in Calabar, Cross River State. Place and Duration of Study: Sample: Markets within Calabar, Cross River State, Nigeria between September and October, 2018. Original Research Article Imalele et al.; JABB, 23(9): 10-16, 2020; Article no.JABB.63082 11 Methodology: A pre-tested semi-structured questionnaire was used for collecting data on factors associated with parasitic contamination of vegetables. 300 vegetable samples were purchased and processed from three different markets in Calabar for examination of parasitic contamination using direct wet mount and zinc sulphate floatation techniques. Results: 15.7% (47/300) of the vegetables sampled were positive for at least one parasite. The species and stages of parasites detected were larvae of Strongyloides, ova of Trichuris trichiura and Hookworm, and cysts of Entamoeba histolytica and Giardia lamblia. Larvae of Strongyloides (11.3%) were the most frequently detected followed by ova of T. trichiura (1.7%) and Hookworm (1.7%) (P = 0.000). Among the five vegetable types sampled, waterleaf (40%) had the highest level of parasitic contamination followed by pumpkin (21.7%). Washing vegetables before display and the means of display were significantly associated with parasitic contamination (P = 0.000). Conclusion: Prevention of contamination is important in reducing food-borne parasitic infections and can be achieved through improved personal hygiene practices of farmers, vendors and consumers, proper washing of vegetables and improved sanitary conditions especially around markets where these vegetables are sold.","PeriodicalId":15023,"journal":{"name":"Journal of Advances in Biology & Biotechnology","volume":"14 1","pages":"10-16"},"PeriodicalIF":0.0,"publicationDate":"2020-12-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"77714222","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}