Journal of Advanced Pharmacy Research最新文献

{"title":"A Comprehensive Review on Genus Zygophyllum","authors":"E. Shawky, Nagwan M. Gabr, M. Elgindi, R. Mekky","doi":"10.21608/APRH.2019.5699.1066","DOIUrl":"https://doi.org/10.21608/APRH.2019.5699.1066","url":null,"abstract":"Objectives: This study aimed to make a broad review of the chemical constituents and biological activities of genus Zygophyllum. Methods: This review covers the peer reviewed articles between 1977 and August, 2018, retrieved from PubMed, Science Direct, Sci-Hub, Springer and Wikipedia Results: In traditional medicine, plants of genus Zygophyllum have been employed and utilized as anti-rheumatic, anti-gout, antidiabetic, anti-hyperlipidemic, antimicrobial, anti-oxidant, antihypertensive, antiseptic, anti-eczema and antidiarrheal. Zygophyllum species have been phytochemically studied leading to the identification of various classes of compounds including triterpenes, flavonoids, saponins, sterols, simple phenolic compounds, and esters. Biological studies on Zygophyllum species have also indicated various bioactive potentials including antioxidant, antidiabetic, antimicrobial antitumor and anti-inflammatory effects. The reported medicinal Zygophyllum species were selected and summarized on basis of their; geographical distribution, traditional uses, chemical constituents and biological activities. Conclusion: It would therefore be important to extensively investigate their phytochemicals and pharmacologically determine their activities for future drug discovery and development.","PeriodicalId":15017,"journal":{"name":"Journal of Advanced Pharmacy Research","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2019-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"73290128","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Structural Characterization of Polyphenolics in Livistona chinensis Using HPLC-PDA-MS","authors":"R. Ahmed, E. Elkhrisy, Walaa El-Kashak, M. Raey, M. Nassar, E. Aboutabl","doi":"10.21608/APRH.2018.6527.1072","DOIUrl":"https://doi.org/10.21608/APRH.2018.6527.1072","url":null,"abstract":"Objectives: This study focused on the identification and characterization of secondary metabolites tentatively from aqueous ethanolic leaf extract of Livistona chinensisby HPLC-PDA-ESI-MS/MS and evaluation of its cytotoxic activity. Methods: The aqueous ethanolic extract was analyzed by high performance liquid chromatography (HPLC) coupled to photodiode array detection of mass spectroscopy (PDA-MS/MS), to detect the secondary metabolites in L. chinensis leaves extract. It was also estimated for its cytotoxicity againsthuman prostate carcinoma (PC3) and hepatocellular liver carcinoma (HepG2) cancer cell lines using SRB (3-(4, 5-dimethyl thiazol-2-yl)-2, 5-diphenyltetrazolium bromide) assay. Results: Forty-two secondary metabolites were tentatively identified; the most major compounds were C-glycoside derivatives of apigenin, luteolin and tricin together with phenolic acids. The 70% alcoholic extract of L. chinensis leaves exhibit more antitumor activity against PC3than against HepG2. Conclusion:L. chinensis is a privileged source of C-flavonoids that revealed the efficiency of HPLC-MS metabolomics in natural products drug discovery.","PeriodicalId":15017,"journal":{"name":"Journal of Advanced Pharmacy Research","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2019-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"83836650","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Evaluation and Characterization of Sildenafil 50 mg Orodispersible Tablets Using Sublimation Technique","authors":"A. Abbas, W. Ibrahim, W. Sakran, A. Badawi","doi":"10.21608/APRH.2018.5126.1065","DOIUrl":"https://doi.org/10.21608/APRH.2018.5126.1065","url":null,"abstract":"Objective: The aim of this work focused on formulation and evaluation of sildenafil 50 mg orodispersible tablets by sublimation technique. Methods: Active ingredient and excipients mixtures were evaluated for physicochemical changes of the drug utilizing FTIR spectroscopy and DSC thermal analysis. Nineteen proposed formulae N1-N19 were prepared by sublimation technique using menthol as a sublimating agent. Three different types of superdisintegrants (sodium starch glycolate, croscarmellose sodium and plasidone XL) were used in three different ratios (3, 6 and 9 % w/w), percentage of inter-granular and intragranular disintegrant. Hydrophilic filler such as mannitol and hydrophobic filler such as microcrystalline cellulose were used in the ratio (1:1, 2:1 and 4:1 w/w).Elimination of bitterness using sucralose as a potent sweetener. Granulation was achieved by alcoholic solution of PVP K25 as binder at Diosna® high shear mixer. Lubricant (hydrophobic magnesium stearate and hydrophilic sodium stearyl fumarate). Un-lubricated granules were characterized for bulk density, tapped density, true density, particle size distribution, Carr'sindex, Hausner ratio, flow rate and angle of repose. Tablets were firstly compressed on rotary machine then subjected to vacuum oven at 60ᵒC for 6 hours. Post compression characterization for tablets after sublimation including content uniformity, average weight, hardness, thickness, In-vitro disintegration, friability, wetting time, assay and dissolution profile of the proposed formulae against the immediate release marketed tablet Viagra ® 50 mg tablet. Results: The formula (N 16) which granulated using 1% PVP k25 with 9% plasidone XL (60% of it is inter-granular while 30% intra-granular), menthol 1%, Microcrystalline cellulose: Mannitol 1:1 and magnesium stearate was the most effective formulation as it showed wetting time of 30.7 seconds, disintegration time of 25 seconds and cumulative % drug release of 92.8 and 95.8 % after 1 and 3 minute respectively. Conclusion: Sildenafil 50 mg ODT successfully was prepared by sublimation technique with better wetting time, disintegration time, assay dissolution profile, hardness and friability.","PeriodicalId":15017,"journal":{"name":"Journal of Advanced Pharmacy Research","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2018-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"87897646","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A Look at Some Medicinal Plants from Sudan-Mini Review","authors":"T. Khider","doi":"10.21608/APRH.2018.18131","DOIUrl":"https://doi.org/10.21608/APRH.2018.18131","url":null,"abstract":"Sudan has large area, with multiculture, habits rich biodiversity and medicinal plants, although was divided in 2011 into two countries still rich with valuable medicinal plants. The objective of the present mini review is to indicate and document some medicinal plants from Sudan and reflect their valuable uses in treating some indigenous and tropical diseases. The present minireview gave a look at some of these plants, mentioning their families, distribution, their local names, their habits, the plant part used for treating diseases and mode of treatment. The medicinal and Aromatic Plants Research Institute (MAPRI) of the National Centre for Research (NCR) plays major role in medicinal and aromatic plants investigation, determination, valuation and documentation in Sudan and contributes with local healers in different regions of Sudan, the rediscovering of these plants is highly needed with contribution of overseas pharmaceuticals companies as natural products have less side effects than industrial drugs.","PeriodicalId":15017,"journal":{"name":"Journal of Advanced Pharmacy Research","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2018-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"91105353","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Acrylamide moiety, a Valuable Fragment in Medicinal Chemistry: Insight into Synthetic Methodologies, Chemical Reactivity and Spectrum of Biological Activities of Acrylamide Derivatives","authors":"Hossam R Elgiushy, S. Hammad, Ashraf S. Hassan, Nageh Aboutaleb, K. Abouzid","doi":"10.21608/APRH.2018.2839.1049","DOIUrl":"https://doi.org/10.21608/APRH.2018.2839.1049","url":null,"abstract":"Acrylamide moiety is a pronounced Michael acceptor that has drawn much interest in a wide array of drugs designed for various therapeutic purposes. Herein we outline different synthetic pathways, scientific bases for its chemical reactivity and how it is functionalized for design of new therapeutic entities, in addition to a brief insight into spectrum of reported biological activities of acrylamide containing compounds up to date.","PeriodicalId":15017,"journal":{"name":"Journal of Advanced Pharmacy Research","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2018-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"72917263","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Efficient processing of Single and Multiple Spectral Variables for Resolution and Quantitation of Paracetamol, Chlorzoxazone and Diclofenac","authors":"M. Hegazy, Mona S. Elshahed, S. Toubar, Marwa I Helmy","doi":"10.21608/APRH.2018.5165.1064","DOIUrl":"https://doi.org/10.21608/APRH.2018.5165.1064","url":null,"abstract":"Objectives: The main aim of this study is to apply smart, simple, rapid and accurate methods for simultaneous determination of Paracetamol (PAR), Chlorzoxazone (CXZ) and Diclofenac potassium (DIC), in their bulk powder and tablet dosage form. Methods: Potent processing methods were used which were based on the application of continuous wavelet transform (CWT) and Savitsky–Golay derivatization (SAVGOL) as univariate spectrophotometric methods, partial least squares (PLS) and synergy interval partial least squares (siPLS) as multivariate methods. Results: PAR, CXZ and DIC were determined in the concentration ranges of 2–30, 2–50 and 2–30 μg/mL, respectively. The regressing and validation parameters of the proposed methods revealed the efficiency of the methods.  The results obtained for the analysis of those drugs by the proposed methods were statistically compared to those obtained by applying a reported high performance liquid chromatographic method. Statistical comparison was done, and no significant difference was found between the proposed methods and the reported one. Conclusion: Successful determination of ternary mixture containing PAR, CXZ and DIC was achieved with no need for tedious sample separation or pre-treatment derivatization which is considered a great benefit in quality control laboratories.","PeriodicalId":15017,"journal":{"name":"Journal of Advanced Pharmacy Research","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2018-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"84271614","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Investigation of Livistona decipiens Leaf Methanol Extract and Evaluation of Antioxidant, Antimicrobial and Cytotoxic Activities","authors":"H. Ibrahim, F. S. Elshaarawy, E. Haggag","doi":"10.21608/APRH.2018.5173.1063","DOIUrl":"https://doi.org/10.21608/APRH.2018.5173.1063","url":null,"abstract":"Objectives: This study aimed to isolate the polyphenolic constituents from the methanol extract of Livistona decipiens Becc leaves and evaluate the antioxidant, cytotoxic and antimicrobial activities of the total methanol extract and ethyl acetate fraction Methods: The ethyl acetate and n-butanol fractions of Livistona decipiens leaf methanol extract were subjected separately to different chromatographic separation techniques. Structures of the isolated compounds were established by different spectroscopic techniques (1H / 13C NMR). Antioxidant activitywas evaluated by DPPH assay, while evaluation of cytotoxicity was done according to MTT cell viability assay. Antimicrobial activity was done by agar diffusion method. Results: seven compounds were isolated from the ethyl acetate and n-butanol fractions, they were identified for the first time from the plant as apigenin-8-C-β-D-glucopyranoside (Vitexin) (1), Quercetin-6-C-β-D-glucopyranoside (2),apigenin-6,8-di-C-β-D-glucopyranoside (Vicenin II) (3), 6-O-methyl Kaempferol 3-O-glucopyranoside (4), luteolin-3-C-gentiobiosyl (5),luteolin-6-C-β-D-glucopyranoside (Isoorientin) (6) and luteolin-8-C-β-D-glucopyranoside (Orientin) (7). The ethyl acetate fraction has shown moderate activity against Gram positive and Gram negatvie bacteria, also it showed moderate antioxidant activity with IC50 = 23 ± 0.74 µg/ml when compared to ascorbic acid IC50 = 14.2 ± 0.35 µg/ml. Also ethyl acetate extract has shown cytotoxic activity on MCF-7 cells (human breast cancer cell line), HepG-2 (human hepatocellular carcinoma) and HeLa cells (human cervical cancer cell line), whereas, the methanol extract has shown lower activity. Conclusion: Livistona decipienshave potential medicinal value being rich in polyphenolics and beingantioxidant, cytotoxic and antimicrobial drug.","PeriodicalId":15017,"journal":{"name":"Journal of Advanced Pharmacy Research","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2018-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"75302917","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Secondary Metabolites and Hepatoprotective Activity of Euphorbia retusa","authors":"Tahany Ghareeb, S. El-toumy, Hussieny Elgendy, E. Haggag","doi":"10.21608/APRH.2018.4312.1060","DOIUrl":"https://doi.org/10.21608/APRH.2018.4312.1060","url":null,"abstract":"Objectives: This study aimed to explore the secondary metabolites from the methanolic extract of Euphorbia retusa forssk, as it is known for its use in folk medicine and for being rich in bioactive molecules. Methods: The methanolic extractwas fractionated by different chromatographic techniques and the structures of the isolated compounds were elucidated by UV, 1H and 13C NMR spectroscopy. Hepatoprotective activity of E. retusa extract was evaluated on CCl4-induced liver damage in rats through biochemical assessment of serum ALT as well as MDA, GSH and NO. It was also evaluated through histopathological study of liver autopsy samples. Results: From the aqueous methanolic extract of Euphorbia retusa forssk. aerial parts seven known phenolic compounds; kamferol-3-O-β-D- glucopyranoside (1), quercetin -3-O-β-D- glucopyranoside (2), 3,3`dimethoxy ellagic acid (3), ellagic acid (4), gallic acid (5), kamferol (6) and quercetin (7) were isolated and identified by chromatographic and spectroscopic analysis. Administration of the extract (100 and 200 mg/kg body weight) significantly decreased the AST and ALT levels, inhibited the CCl4 -induced elevated levels of NO and MDA and increased the level of hepatic GSH. A comparative histopathological study of liver exhibited almost nearly normal architecture as compared to toxicant group. Conclusion: Euphorbia retusaextract has shown to have hepatoprotective activityon CCl4-induced liver damage in rats which might be attributed to its phenolic contents.","PeriodicalId":15017,"journal":{"name":"Journal of Advanced Pharmacy Research","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2018-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"88156678","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A Novel Compound and Biological Evaluation of Phytoconstituents Isolated from Erythrina corallodendron L. Flowers","authors":"M. Aboelmagd, A. Said, S. Ross, E. Haggag","doi":"10.21608/APRH.2018.3926.1059","DOIUrl":"https://doi.org/10.21608/APRH.2018.3926.1059","url":null,"abstract":"Objective: This study aimed at phytochemical investigation of the 70% alcoholic extract of Erythrina corallodendron L. flowers and biological evaluation of the isolated compounds for their activity as antiprotozoal drugs also evaluation the binding affinity to opioid and cannabinoid receptors as well as the inhibition activity against monoamine oxidase (MAO) enzymes. Method: The 70% alcoholic extract was subjected to successive column chromatographic (CC) separations using silica gel normal phase, reversed phase RP-18, Diaion HP-20, and Sephadex LH-20. The structural elucidation of the isolated compounds was achieved using HR-ESI-MS, UV, 1D and 2D NMR spectroscopic analysis. The isolated compounds were screened in vitro for the binding affinity to opioid and cannabinoid receptors using receptor binding assay as well as the inhibition activity against MAO enzymes using kynuramine deamination assay, while their antiprotozoal activity was evaluatedusing parasitelactate dehydrogenase serum assay (pLDH). Results: The phytochemical evaluation of the alcoholic extract of E.corallodendron flowers, afforded the isolation of an indole alkaloid Hypaphorine 1, a new flavonoid glucoside; Kaempferol-3-O-α-sophoroside 2 and three known flavonoid C-glycosides vis;, Neoschaftoside 3, Isoschaftoside 4 and Vicenin-II 5. Compounds 3 and 4 are reported for the first time from genus Erythrina. Compounds 4 and 5 showed significant antimalarial activity both with IC50 value 1.7µg/mL against (D6) strain and with IC50 1.4 and 1.1 µg/mL against (W2) strain, respectively. Compound 3 showed selective inhibition to MAO-B with IC50 value of 32.08 µM and selective index (SI) > 3.12. Conclusion: The significant antiplasmodial activity of compounds 4 and 5 correlated the known antimalarial activity of different Erythrina species to flavonoid C-glycosides, Also compounds 3 and 4 are position isomers but exhibited different response against MAO-B which gives indication about the selectivity pattern of the flavonoid C-glycosides with MAO-B receptor subtype.","PeriodicalId":15017,"journal":{"name":"Journal of Advanced Pharmacy Research","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2018-09-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"88238881","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"HPLC investigation of carbohydrates and phenolic constituents of Livistona decipiens and Livistona australis leaves and assessment of their ulceroprotective activity","authors":"F. S. Elshaarawy, S. Mina, Nagwan M. Gabr, S. M. Abdel-Khalik, Rehab Kamel, H. Ibrahim, E. Haggag","doi":"10.21608/APRH.2018.8068","DOIUrl":"https://doi.org/10.21608/APRH.2018.8068","url":null,"abstract":"Objectives: This study aimed to compare two Livistona species; Livistona decipiens Becc and Livistona australis Mart for their phenolic and carbohydrate contents and for their protective activity against ulcerative colitis. Methods: A high performance liquid chromatographic (HPLC) technique with inline connected to photo diode array detector (DAD) and electro array (EA) used for detection of the polyphenolic contents. Also HPLC with refractive index detection (HPLC-RI) was used to determine and to quantify carbohydrate contents before and after partial acid hydrolysis, using 0.2 N H2SO4, for 3 h at 100°C, for each of the defatted methanol extract concentrate. Protective activity against ulcerative colitis was evaluated by acetic acid inducing-ulcers method for both methanol extracts of both species. Results: Both investigated Livistona species are rich in polyphenolic constituents, showing a great similarity. The major flavonoid compound in both species was luteolin-6-C-arabinoside-8-C-glucoside and the major aglycone was acacetin, while the major phenolic acid in both species was ellagic acid. However there was a difference in the carbohydrate content between the two species, the main sugars, before hydrolysis, in L. decipiens were arabinose, mannose and sucrose, while the main sugars in L. australis were glucose, fructose and maltose. However, stachyose was the major polysaccharide obtained after partial acid hydrolysis. L. australis showed protective activity against ulcerative colitis in lower dose 500 mg/kg when compared to L. decipiens that only showed effectiveness at a doubled dose 1000 mg/kg. Conclusion: Both Livistona species have potential medicinal value being rich in polyphenolic and polysaccharide contents and having protective activity against ulcerative colitis.","PeriodicalId":15017,"journal":{"name":"Journal of Advanced Pharmacy Research","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2018-06-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"79586400","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}