Journal of Advanced Pharmacy Research最新文献

{"title":"Highly Sensitive, Selective and Validated Spectrofluorimetric Assay for Novel Oxazolidinone Antibiotic: Tedizolid Phosphate in Pharmaceutical Dosage Form and Human Plasma","authors":"Basma M. Tawfik, M. Rizk, M. Sultan, R. El-Eryan","doi":"10.21608/aprh.2021.77133.1132","DOIUrl":"https://doi.org/10.21608/aprh.2021.77133.1132","url":null,"abstract":"Objectives: In this work we presented a highly sensitive, selective and validated method for determination of tedizolid phosphate (TEDP) antibiotic, based on its native fluorescence in aqueous solution. Methods: The maximum fluorescence intensity was measured at 408 nm after excitation at 298 nm after optimization of all experimental conditions. Results: The measured fluorescence was directly proportional to the concentration of the drug over the range of 2-30 ng/mL with a limit of detection of 0.13 and limit of quantification of 0.44 ng/mL The method succeeded to determine TEDP in its pharmaceutical dosage form and in human plasma with mean % recovery of 100.49 ±1.32 and 99.40 ± 2.18 respectively. Conclusion: The developed data was found to be with a good agreement with a valid method. The method was validated according to ICH guidelines for determination of the drug in its pure form and dosage form and according to FDA Guidance for Industry, Bioanalytical Method Validation for determination of TEDP in human plasma.","PeriodicalId":15017,"journal":{"name":"Journal of Advanced Pharmacy Research","volume":"8 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"89706703","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Mangifera indica Leaves Extracts Mitigate Experimentally Induced Oxidative Stress and Iron-Overload via Iron Chelation and Modulation of HO-1, Nrf2, and MMP-9","authors":"Ahmed G. Abd Elhameed, Sara N. Suliman, Marwa Elsbaey, Mai M. Elnaggar, F. Badria","doi":"10.21608/aprh.2020.42333.1115","DOIUrl":"https://doi.org/10.21608/aprh.2020.42333.1115","url":null,"abstract":"Objective: The current study aimed to investigate and compare the efficiency of the total extract and ethyl acetate (EtOAc) extract of Mangifera indica leaves to attenuate iron overload-induced hepatic and splenic dysfunctions, using an in-vivo iron-overload rat model. Methods: Rats received water supplemented with iron for 2 months, followed by treatment with either total extract or EtOAc extract of M. indica leaves for an extra 2 months. Results: Rats treated with EtOAc fraction of M. indica leaves, and the total extract, had significantly diminished iron accumulation within hepatic and splenic tissues. Both extracts normalized the hepatic enzymatic and non-enzymatic biomarkers like alkaline phosphatase (ALP), alanine aminotransferase (AST), and total bilirubin. Moreover, they restored the oxidants/antioxidants balance within the liver and spleen tissues. Additionally, they suppressed HO-1 levels, increased Nrf2 expression, and downregulated MMP-9 expression. Conclusion: Briefly, M. indica extracts showed iron-chelation, antioxidant, and antifibrotic potentials. These findings pointed out the usefulness of the mango leaves as a natural, affordable by-product in managing iron overload complications. However, the results also demonstrated that EtOAc extract is more accountable for such activities.","PeriodicalId":15017,"journal":{"name":"Journal of Advanced Pharmacy Research","volume":"30 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2020-12-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"72850225","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Development of a Method for the Determination of Miconazole in Water Samples Using Gas Chromatography Mass Spectrometry","authors":"M. Ibrahim, A. Y. Mohamed, A. A. Ahmed","doi":"10.21608/aprh.2019.15686.1089","DOIUrl":"https://doi.org/10.21608/aprh.2019.15686.1089","url":null,"abstract":"This paper describes an enhanced gas chromatography mass spectrometry (GC-MS) strategy for the analysis of Miconazole in water samples. In this study, determination of Miconazole has been carried out according to standard method for water and waste water analysis. Samples of collected water were agriculture stream water, River Nile water and Hospital waste water samples from El-Gharbia governorate in Egypt. Miconazole was extracted by solid - liquid extraction and analyzed by GC-MS. The chromatographic separation was performed using a ZB5 column (30 m ×0.53 mm, 1.50 µm), and helium as a carrier gas. The limit of detection and limit of quantification for Miconazole were 0.75and 2.50 ng/mL respectively. The intra- and inter-day precisions were lower than 0.85% while the accuracy ranged from 98.55% to 101.53. Finally, solid phase extraction (SPE) in combination with GC-MS is a sensitive and effective method for the determination of Miconazole in water samples.","PeriodicalId":15017,"journal":{"name":"Journal of Advanced Pharmacy Research","volume":"64 49 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2019-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"75403658","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Pharmacokinetics of Tacrolimus in Egyptian Liver Transplant Recipients: Role of the Classic Co-variables","authors":"Abdel-hamid Ebid, S. Mohamed, A. Mira, A. Saleh","doi":"10.21608/aprh.2019.14237.1087","DOIUrl":"https://doi.org/10.21608/aprh.2019.14237.1087","url":null,"abstract":"Objectives: This work was performed to study the pharmacokinetics of tacrolimus in adult liver transplant recipients after optimization of all the known classic factors contributing to inter-patient variability in whole blood tacrolimus levels. Also, to detect if any variability in whole blood tacrolimus levels still exists or this variability is only a function of the classic co-variables so that their optimization will diminish or eliminate it. Methods: Twenty-Six male patients with end-stage liver disease undergoing living donor liver transplantation were selected from the Gastroenterology Department of the International Medical Center, Cairo, Egypt, were enrolled in the study. A patient is initially considered to be a candidate for this study when tacrolimus was indicated as a part of a triple immune suppressive regimen with mycophenolate mofetil and prednisolone. Patients were selected to have non-significant variations in their demographics and pretreatment clinical data. Blood samples were drawn from each patient before the morning dose at specified intervals and the whole blood was assayed for tacrolimus, using Chemiluminescent Microparticle Immunoassay method (CMIA). Six months after liver transplantation, patients were classified into 3 groups based on their tacrolimus trough levels; normalized by its daily dose (C/D ratio), into fast, intermediate and slow metabolizers. Results: The results revealed unpredictable variability in whole blood tacrolimus levels among patients at each sampling time and a marked inter-patient variability in mean whole blood tacrolimus levels among individuals throughout the six months post transplantation period, (P value: <0.0001). Considerable inter-patient variability was also evident in tacrolimus pharmacokinetics. During 1st month post-transplant, tacrolimus C/D ratio varied from 0.53 to 12.2 (ng/ml*1/mg) and tacrolimus oral clearance (CL/F) varied from 3.4 to 79.4 L/hr. At 3rd month post-transplant, tacrolimus C/D ratio varied from 0.78 to 8.50 (ng/ml*1/mg) and tacrolimus CL/F varied from 4.9 to 53.2 L/hr. At 6th month post-transplant, tacrolimus C/D ratio varied from 0.73 to 7.10 (ng/ml*1/mg) and tacrolimus CL/F varied from 5.9 to 56.8 L/hr. The overall mean C/D ratio and oral clearance also showed a great variability among patients with a mean of 2.80±1.89 (CV: 67.5%) and 21.3±12.9 (CV:60.7%), respectively. Conclusion: The variability in whole blood tacrolimus concentrations and tacrolimus pharmacokinetics existed in spite of careful patient selection and optimization of all the classic co-variables known to affect tacrolimus concentrations, suggesting the presence of other unstudied factors; the recently evolving genetic factors might contribute to this variability. It is recommended to still considering therapeutic drug monitoring as an integral part of tacrolimus therapy to control variations in response until the discovery of a model that considers all the expected covariates to predict the respo","PeriodicalId":15017,"journal":{"name":"Journal of Advanced Pharmacy Research","volume":"7 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2019-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"78690080","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Phytochemical Investigation and Assessment of Antioxidant, Antimicrobial and Cytotoxic Activities of the Root Bark Chrozophora oblongifolia (Delile) Spreng. (Euphorbiaceae)","authors":"M. R. Kamel, A. M. Nafady, A. Hassanein, R. Ragaey, E. Haggag","doi":"10.21608/aprh.2019.17219.1090","DOIUrl":"https://doi.org/10.21608/aprh.2019.17219.1090","url":null,"abstract":"antioxidant, antimicrobial and cytotoxic activities of the total methanolic extract and different fractions of Chrozophora oblongifolia root bark. Methods: Phytochemical screening of Chrozophora oblongifolia was performed using specific test for each class of compounds. Different chromatographic techniques were used to isolate and purify compounds and their structures were elucidated by using different 1D-NMR spectroscopy (1H NMR and 13C NMR). The antioxidant activity was evaluated using DPPH radical scavenging assay, antimicrobial activity was done by standard agar well diffusion assay and cytotoxic activity was performed by using MTT colorimetric assay method. Results: Phytochemical investigation of chrozophora oblongifolia revealed the presence ofcarbohydrates and / or glycosides, sterols and or triterpene, alkaloids, tannins and saponins and the absence of flavonoids and anthraquinones. After fractionation,   methyl gallate (1), gallic acid (2) and β-sitosterol-3-O-β-D- glucopyranoside (3) were isolated from ethyl acetate fractions. The result of the antioxidant activity showed that, the ethyl acetate fraction showed highest antioxidant activity followed by n-butanol and total methanolic extract. The result of the antimicrobial activity showed that, the total methanolic extract and ethyl acetate fraction showed moderate activity against Staphylococcus aureus, Vancomycin resistant Staphylococcus aureus and Candida albicans. The result of the cytotoxic activity showed that, the methylene chloride fraction followed by the total methanolic extract showed the highest cytotoxic activity against (MCF-7 and Huh-7) cancer cell lines. Conclusion: The root bark of Chrozophora oblongifolia is a rich source of different classes of active constituents as phenolics. The total extract and some fractions of Chrozophora oblongifolia showing antioxidant and antimicrobial and cytotoxic activities.","PeriodicalId":15017,"journal":{"name":"Journal of Advanced Pharmacy Research","volume":"46 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2019-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"85148087","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A Phytochemical and Biological Review on Plants of The family Aizoaceae","authors":"Khayrya A. Youssif, Ali M Elshamy, M. Rabeh, Nagwan M. Gabr, E. Haggag","doi":"10.21608/aprh.2019.12303.1083","DOIUrl":"https://doi.org/10.21608/aprh.2019.12303.1083","url":null,"abstract":"Objectives: This study is aimed to be a comprehensive review of the phytochemical constituents and biological activities of Aizoaceae family plants (Mesembryanthemaceae). Methods: This study is covering articles between 1969 and 2018, reviewed from internationally accepted databases and scientific data from scientific Journals. Results: Phytochemically studied plants of family Aizoaceae have shown the presence of various classes of compounds including; alkaloids, triterpenes, sterols, lignans, phenolic compounds, betacyanins, and essential oils. Biological studies on plants of family Aizoaceae have indicated various bioactive potentials including antioxidant, antidiabetic, antimicrobial, antitumor, hepatoprotective, anti-inflammatory and other effects. The reported medicinal plants of family Aizoaceae were selected and summarized on the basis of their; phytochemical constituents and biological activities. Conclusion: The results of this study may inspire further ethno-botanical and ethno-pharmacological research and investigations toward drug discovery.","PeriodicalId":15017,"journal":{"name":"Journal of Advanced Pharmacy Research","volume":"47 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2019-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"79888774","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Phytochemical Investigation and Assessment of Antioxidant and Antimicrobial Activities of Phagnalon barbeyanum Aerial Parts","authors":"M. R. Kamel, A. M. Nafady, A. Hassanein, R. Ragaey, E. Haggag","doi":"10.21608/APRH.2019.13691.1084","DOIUrl":"https://doi.org/10.21608/APRH.2019.13691.1084","url":null,"abstract":"Objectives: The present study aimed to investigate and isolate different phytochemical constituents and assess the antioxidant and antimicrobial activities of the total methanol extract and different fractions of Phagnalon barbeyanum aerial parts. Methods: Phytochemical screening of Phagnalon barbeyanum aerial parts was performed using specific test for each class of compounds. Different chromatographic techniques were used to isolate and purify compounds and their structures were elucidated by using different spectral techniques (1H NMR and 13C NMR). The antioxidant activity was evaluated by DPPH radical scavenging assay and antimicrobial activity was done by standard agar well diffusion assay. Results: Phytochemical investigation of Phagnalon barbeyanum aerial partswas done forthe presence ofcarbohydrates and or glycosides, sterols and or triterpene, flavonoids, tannins and saponins and revealed the absence of alkaloids and anthraquinones. Compounds; β-sitosterol (1), apigenin (2) and β-sitosterol-3-O-β-D- glucopyranoside (3) were isolated from methylene chloride and ethyl acetate fractions. The ethyl acetate fraction showed highest antioxidant activity followed by n-butanol and methylene chloride fractions. The total methanol extract and n-hexane fractions showed lowest antioxidant activity. The ethyl acetate fraction and total methanol extract showed moderate activity against Staphylococcus aureus and Vancomycin resistant Staphylococcus aureus. Conclusion: The aerial parts of Phagnalon barbeyanum is a rich source of different classes of active constituents as phenolics. The total methanol extract of Phagnalon barbeyanum aerial parts and some of its fractionated concentrates could be considered as antioxidant and antimicrobial agents.","PeriodicalId":15017,"journal":{"name":"Journal of Advanced Pharmacy Research","volume":"39 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2019-07-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"75644776","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Validated HPLC Determination of the Potential Anti-Helicobacter pylori, Lepidine, in Lepidium sativum Seeds Assessed by Molecular Docking Study","authors":"A. Haddad, W. Al-Shareef, S. Eid","doi":"10.21608/APRH.2019.14036.1085","DOIUrl":"https://doi.org/10.21608/APRH.2019.14036.1085","url":null,"abstract":"Background: Nearly 50 % of the world residents are known to be infected with Helicobacter pylori which is the main cause of peptic ulcer and gastric cancer. Since resistance to the currently used treatments, other alternative approaches such as combination with natural products should be tried. Objectives: In this work, validated HPLC-UV method was developed for quantification of lepidine content in various extracts and crude oil of Lepidium sativum seeds. Furthermore, antibacterial activity of lepidine against H. pylori was compared with standard antibiotics supported by molecular docking study. Methods: Different solvents were used to extract lepidine as well as the contents were determined using HPLC-UV method. Anti-Helicobacter pylori activity was examined using agar diffusion and dilution methods. Molecular docking study was done on H. pylori phosphoribosyltransferase enzyme. Results: The highest content of lepidine (316.91 µg mL-1) was found in n-hexane extract. Lepidine exhibit antibacterialeffect (MIC = 6.25 µg mL-1) against H. pylori clinical isolates. The ability of lepidine to interact with amino acids in the phosphoribosyl transferase binding site might rationalize its observed activity. Conclusion: Our results demonstrate for the first time the anti-Helicobacter pylori activity of lepidine, which could therefore be developed as viable nutraceutical agent. Further investigations are required to formulate suitable pharmaceuticals to combat with the H. pylori infections on clinical grounds.","PeriodicalId":15017,"journal":{"name":"Journal of Advanced Pharmacy Research","volume":"40 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2019-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"77059629","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"In Vitro Anticlastogenic and Antioxidant properties of extracted and Pure form of Curcumin against Mitomycin C in Human Peripheral Leukocytes","authors":"Puspal De, M. Mukhopadhyay","doi":"10.21608/APRH.2019.10241.1080","DOIUrl":"https://doi.org/10.21608/APRH.2019.10241.1080","url":null,"abstract":"Objectives: Turmeric is native to India and is widely used in Indian cuisine. Curcumin, the active principle of turmeric used primarily as a coloring agent in food, drug and cosmetics. The medicinal properties of curcumin are well known in ancient Indian Ayurvedic Medicine. Mitomycin C (MMC) is an antineoplastic agent used to fight a number of different cancers including cancer of the stomach, colon, rectum, pancreas, breast, lung, uterus, cervix, bladder, head, neck, eye and esophagus. It is a potent DNA cross-linker. But it has severe side effect, the prolonged use of the drug may result in permanent bone marrow damage and other various types of secondary tumors in normal cells. The present study was conducted to examine the anticlastogenic action of extracted curcumin from dry turmeric and pure curcumin against the MMC-induced chromosomal aberrations. For this purpose, in the present study, clastogenic parameter like chromosomal aberration test was conducted in peripheral human leukocytes. Methods: The antioxidant property of both extracted and pure curcumin was also evaluated by phosphomolybdenum method. Results: Our results demonstrated that, extracted as well as pure Curcumin, a strong antioxidant phyto-molecule is effective in counteracting the clastogenicity in human leukocyte in vitro. Conclusion: These results suggest that the use of turmeric in diet may be an effective protection against the health crisis generated by harmful agents.","PeriodicalId":15017,"journal":{"name":"Journal of Advanced Pharmacy Research","volume":"1 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2019-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"89415104","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Synthesis and Biological Evaluation of Certain α,β-Unsaturated Ketones and their Corresponding Fused Pyridines","authors":"A. Hassan, H. el-Hifnawi, W. Ahmed","doi":"10.21608/aprh.2019.10458.1081","DOIUrl":"https://doi.org/10.21608/aprh.2019.10458.1081","url":null,"abstract":"","PeriodicalId":15017,"journal":{"name":"Journal of Advanced Pharmacy Research","volume":"159 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2019-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"91448397","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}