Journal of Amino Acids最新文献_第2页

Photon atomic parameters of nonessential amino acids for radiotherapy and diagnostics. 放射治疗和诊断用非必需氨基酸的光子原子参数。

Journal of Amino Acids Pub Date : 2014-01-01 Epub Date: 2014-12-07 DOI: 10.1155/2014/434519

Ertuğrul O Bursalıoğlu, Orhan İçelli, Begüm Balkan, H Birtan Kavanoz, Mustafa Okutan

引用次数: 3

Lauryl-poly-L-lysine: A New Antimicrobial Agent? 月桂基聚赖氨酸:一种新的抗菌剂?

Journal of Amino Acids Pub Date : 2014-01-01 Epub Date: 2014-02-23 DOI: 10.1155/2014/672367

Laetitia Vidal, Véronique Thuault, Arturo Mangas, Rafael Coveñas, Anne Thienpont, Michel Geffard

{"title":"Lauryl-poly-L-lysine: A New Antimicrobial Agent?","authors":"Laetitia Vidal, Véronique Thuault, Arturo Mangas, Rafael Coveñas, Anne Thienpont, Michel Geffard","doi":"10.1155/2014/672367","DOIUrl":"https://doi.org/10.1155/2014/672367","url":null,"abstract":"The development of multiple antibiotic resistance is a global problem. It is necessary to find new tools whose mechanisms of action differ from those of currently used antibiotics. It is known that fatty acids and cationic polypeptides are able to fight bacteria. Here, we describe the synthesis of fatty acids linked to a polypeptide with antibacterial activity. The linkage of fatty acids to a polypeptide is reported to increase the antibacterial effect of the linked fatty acid in comparison with free fatty acids (FA) or free poly-L-lysine (PLL) or a mixture of both (FA free + PLL free). A number of C6-C18 fatty acids were linked to PLL to obtain new synthetic products. These compounds were assessed in vitro to evaluate their antibacterial activity. Some fatty acid-PLLs showed a good ability to fight bacteria. Their bactericidal activity was evaluated, and, lauryl linked to PLL was found to be the most active product against both Gram-positive and Gram-negative bacteria. This new active component showed a good degree of specificity and reproducibility and its minimum inhibitory concentration (MIC) was comparatively good. The antibacterial activity of the lauryl-PLL compound suggests that it is a new and promising antimicrobial agent. ","PeriodicalId":14901,"journal":{"name":"Journal of Amino Acids","volume":"2014 ","pages":"672367"},"PeriodicalIF":0.0,"publicationDate":"2014-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1155/2014/672367","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"32200111","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 19

Immune activation and inflammation in patients with cardiovascular disease are associated with higher phenylalanine to tyrosine ratios: the ludwigshafen risk and cardiovascular health study. 心血管疾病患者的免疫激活和炎症与较高的苯丙氨酸与酪氨酸比率相关:路德维希港风险和心血管健康研究

Journal of Amino Acids Pub Date : 2014-01-01 Epub Date: 2014-02-10 DOI: 10.1155/2014/783730

Christian Murr, Tanja B Grammer, Andreas Meinitzer, Marcus E Kleber, Winfried März, Dietmar Fuchs

{"title":"Immune activation and inflammation in patients with cardiovascular disease are associated with higher phenylalanine to tyrosine ratios: the ludwigshafen risk and cardiovascular health study.","authors":"Christian Murr, Tanja B Grammer, Andreas Meinitzer, Marcus E Kleber, Winfried März, Dietmar Fuchs","doi":"10.1155/2014/783730","DOIUrl":"https://doi.org/10.1155/2014/783730","url":null,"abstract":"Higher serum neopterin is associated with increased mortality in patients with coronary artery disease (CAD). Preferentially Th1-type cytokine interferon- γ stimulates neopterin production by GTP cychlohydrolase I (GCH-I) in parallel in monocyte-derived macrophages and dendritic cells. In other cells, activation of GCH-I leads to the formation of 5,6,7,8-tetrahydrobiopterin (BH4), the necessary cofactor of amino acid hydroxylases like phenylalanine 4-hydroxylase (PAH). Serum concentrations of phenylalanine, tyrosine, neopterin, and high sensitivity C-reactive protein (hsCRP) were measured in 1196 patients derived from the LUdwigshafen RIsk and Cardiovascular Health (LURIC) study, a cohort study among patients referred for coronary angiography. The phenylalanine to tyrosine ratio (Phe/Tyr) served as an estimate of phenylalanine hydroxylase (PAH) enzyme activity. Serum concentrations of phenylalanine and tyrosine and of Phe/Tyr did not differ between individuals with or without CAD (Welch's t-test: P = n.s.). Higher neopterin and hsCRP concentrations were observed in CAD patients compared to controls (P < 0.0001) and they correlated with Phe/Tyr (Spearman's rank correlation for neopterin: r s = 0.216 and hsCRP: r s = 0.122; both of P < 0.0001) concentrations. In conclusion, immune activation is associated with higher Phe/Tyr in CAD patients. Data indicates subnormal PAH activity which might be involved in the precipitation of neuropsychiatric symptoms in patients. ","PeriodicalId":14901,"journal":{"name":"Journal of Amino Acids","volume":"2014 ","pages":"783730"},"PeriodicalIF":0.0,"publicationDate":"2014-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1155/2014/783730","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"32200112","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 69

Interaction of ingested leucine with glycine on insulin and glucose concentrations. 摄入亮氨酸与甘氨酸对胰岛素和葡萄糖浓度的相互作用。

Journal of Amino Acids Pub Date : 2014-01-01 Epub Date: 2014-07-10 DOI: 10.1155/2014/521941

Jennifer F Iverson, Mary C Gannon, Frank Q Nuttall

{"title":"Interaction of ingested leucine with glycine on insulin and glucose concentrations.","authors":"Jennifer F Iverson, Mary C Gannon, Frank Q Nuttall","doi":"10.1155/2014/521941","DOIUrl":"https://doi.org/10.1155/2014/521941","url":null,"abstract":"The majority of individual amino acids increase insulin and attenuate the plasma glucose response when ingested with glucose. Objective. To determine whether ingestion of two amino acids simultaneously, with glucose, would result in an additive effect. Leucine (Leu) and glycine (Gly) were chosen because they were two of the most potent glucose-lowering amino acids when given individually. Materials and Methods. Nine subjects received test items on four separate days. The first was a water control, then 25 g glucose, or Leu + Gly (1 mmol/kg fat-free mass each) ±25 g glucose, in random order. Glucose, insulin, and glucagon were measured frequently for 2.5 hours. Net areas were calculated. Results. The glucose area response decreased by 66%. The insulin area response increased by 24% after ingestion of Leu + Gly + glucose compared to ingestion of glucose alone. The decrease in glucose response was not additive; the increase in insulin response was far less than additive when compared to previously published individual amino acid results. The glucagon concentration remained unchanged. Conclusion. There is an interaction between Leu and Gly that results in a markedly attenuated glucose response. This occurred with a very modest increase in insulin response. Changes in glucagon response could not explain the results. The mechanism is unknown. ","PeriodicalId":14901,"journal":{"name":"Journal of Amino Acids","volume":"2014 ","pages":"521941"},"PeriodicalIF":0.0,"publicationDate":"2014-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1155/2014/521941","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"32583944","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 8

In Silico Analysis of β-Galactosidases Primary and Secondary Structure in relation to Temperature Adaptation. 与温度适应有关的β-半乳糖苷酶一级和二级结构的硅分析。

Journal of Amino Acids Pub Date : 2014-01-01 Epub Date: 2014-03-24 DOI: 10.1155/2014/475839

Vijay Kumar, Nikhil Sharma, Tek Chand Bhalla

{"title":"In Silico Analysis of β-Galactosidases Primary and Secondary Structure in relation to Temperature Adaptation.","authors":"Vijay Kumar, Nikhil Sharma, Tek Chand Bhalla","doi":"10.1155/2014/475839","DOIUrl":"https://doi.org/10.1155/2014/475839","url":null,"abstract":"β -D-Galactosidases (EC 3.2.1.23) hydrolyze the terminal nonreducing β -D-galactose residues in β -D-galactosides and are ubiquitously present in all life forms including extremophiles. Eighteen microbial β -galactosidase protein sequences, six each from psychrophilic, mesophilic, and thermophilic microbes, were analyzed. Primary structure reveals alanine, glycine, serine, and arginine to be higher in psychrophilic β -galactosidases whereas valine, glutamine, glutamic acid, phenylalanine, threonine, and tyrosine are found to be statistically preferred by thermophilic β -galactosidases. Cold active β -galactosidase has a strong preference towards tiny and small amino acids, whereas high temperature inhabitants had higher content of basic and aromatic amino acids. Thermophilic β -galactosidases have higher percentage of α -helix region responsible for temperature tolerance while cold loving β -galactosidases had higher percentage of sheet and coil region. Secondary structure analysis revealed that charged and aromatic amino acids were significant for sheet region of thermophiles. Alanine was found to be significant and high in the helix region of psychrophiles and valine counters in thermophilic β -galactosidase. Coil region of cold active β -galactosidase has higher content of tiny amino acids which explains their high catalytic efficiency over their counterparts from thermal habitat. The present study has revealed the preference or prevalence of certain amino acids in primary and secondary structure of psychrophilic, mesophilic, and thermophilic β -galactosidase. ","PeriodicalId":14901,"journal":{"name":"Journal of Amino Acids","volume":"2014 ","pages":"475839"},"PeriodicalIF":0.0,"publicationDate":"2014-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1155/2014/475839","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"32310014","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 23

Design and synthesis of novel isoxazole tethered quinone-amino Acid hybrids. 新型异恶唑系缚醌-氨基酸杂合体的设计与合成。

Journal of Amino Acids Pub Date : 2014-01-01 Epub Date: 2014-11-19 DOI: 10.1155/2014/721291

P Ravi Kumar, Manoranjan Behera, M Sambaiah, Venu Kandula, Nagaraju Payili, A Jaya Shree, Satyanarayana Yennam

引用次数: 6

Low-Vacuum Deposition of Glutamic Acid and Pyroglutamic Acid: A Facile Methodology for Depositing Organic Materials beyond Amino Acids. 谷氨酸和焦谷氨酸的低真空沉积:一种除氨基酸外沉积有机物质的简便方法。

Journal of Amino Acids Pub Date : 2014-01-01 Epub Date: 2014-09-01 DOI: 10.1155/2014/434056

Iwao Sugimoto, Shunsaku Maeda, Yoriko Suda, Kenji Makihara, Kazuhiko Takahashi

{"title":"Low-Vacuum Deposition of Glutamic Acid and Pyroglutamic Acid: A Facile Methodology for Depositing Organic Materials beyond Amino Acids.","authors":"Iwao Sugimoto, Shunsaku Maeda, Yoriko Suda, Kenji Makihara, Kazuhiko Takahashi","doi":"10.1155/2014/434056","DOIUrl":"https://doi.org/10.1155/2014/434056","url":null,"abstract":"Thin layers of pyroglutamic acid (Pygl) have been deposited by thermal evaporation of the molten L-glutamic acid (L-Glu) through intramolecular lactamization. This deposition was carried out with the versatile handmade low-vacuum coater, which was simply composed of a soldering iron placed in a vacuum degassing resin chamber evacuated by an oil-free diaphragm pump. Molecular structural analyses have revealed that thin solid film evaporated from the molten L-Glu is mainly composed of L-Pygl due to intramolecular lactamization. The major component of the L-Pygl was in β-phase and the minor component was in γ-phase, which would have been generated from partial racemization to DL-Pygl. Electron microscopy revealed that the L-Glu-evaporated film generally consisted of the 20 nm particulates of Pygl, which contained a periodic pattern spacing of 0.2 nm intervals indicating the formation of the single-molecular interval of the crystallized molecular networks. The DL-Pygl-evaporated film was composed of the original DL-Pygl preserving its crystal structures. This methodology is promising for depositing a wide range of the evaporable organic materials beyond amino acids. The quartz crystal resonator coated with the L-Glu-evaporated film exhibited the pressure-sensing capability based on the adsorption-desorption of the surrounding gas at the film surface. ","PeriodicalId":14901,"journal":{"name":"Journal of Amino Acids","volume":"2014 ","pages":"434056"},"PeriodicalIF":0.0,"publicationDate":"2014-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1155/2014/434056","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"32694515","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 2

Tissue taurine depletion alters metabolic response to exercise and reduces running capacity in mice. 组织牛磺酸耗竭改变了小鼠对运动的代谢反应，降低了小鼠的跑步能力。

Journal of Amino Acids Pub Date : 2014-01-01 Epub Date: 2014-11-12 DOI: 10.1155/2014/964680

Takashi Ito, Natsumi Yoshikawa, Stephen W Schaffer, Junichi Azuma

{"title":"Tissue taurine depletion alters metabolic response to exercise and reduces running capacity in mice.","authors":"Takashi Ito, Natsumi Yoshikawa, Stephen W Schaffer, Junichi Azuma","doi":"10.1155/2014/964680","DOIUrl":"https://doi.org/10.1155/2014/964680","url":null,"abstract":"Taurine is a sulfur-containing amino acid found in very high concentration in skeletal muscle. Taurine deficient mice engineered by knocking out the taurine transporter gene exhibit skeletal muscle wasting, structural defects, and exercise intolerance. In the present study, we investigated the mechanism underlying the development of metabolic abnormalities and exercise intolerance in muscle of the TauTKO phenotype. Running speed and endurance time of TauTKO mice were lower than those of control mice. Blood lactate level was elevated by >3-fold during treadmill running in TauTKO mice but remained largely unaltered by exercise in WT mice. Blood glucose was cleared faster during treadmill running in TauTKO mice than WT mice. AMP-activated kinase (AMPK) β-2 subunit was reduced in TauTKO muscle concomitant with a reduction in α1 and α2 subunits of AMPK. The level of PPARα and its targets, Gpx3, Cpt2, and Echs1, were also decreased in TauTKO muscle. Collectively, taurine depletion impairs metabolic adaptation to exercise in skeletal muscle, a phenomenon associated with a downregulation of AMPK and diminished NADH utilization by the mitochondrial respiratory chain. These findings suggest a crucial role of taurine in regulating energy metabolism in skeletal muscle of exercising TauTKO mice, changes that contribute to impaired exercise endurance. ","PeriodicalId":14901,"journal":{"name":"Journal of Amino Acids","volume":"2014 ","pages":"964680"},"PeriodicalIF":0.0,"publicationDate":"2014-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1155/2014/964680","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"32884255","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 52

Development of a novel cysteine sulfinic Acid decarboxylase knockout mouse: dietary taurine reduces neonatal mortality. 新型半胱氨酸亚磺酸脱羧酶敲除小鼠的发展:饮食牛磺酸降低新生儿死亡率。

Journal of Amino Acids Pub Date : 2014-01-01 Epub Date: 2014-02-03 DOI: 10.1155/2014/346809

Eunkyue Park, Seung Yong Park, Carl Dobkin, Georgia Schuller-Levis

{"title":"Development of a novel cysteine sulfinic Acid decarboxylase knockout mouse: dietary taurine reduces neonatal mortality.","authors":"Eunkyue Park, Seung Yong Park, Carl Dobkin, Georgia Schuller-Levis","doi":"10.1155/2014/346809","DOIUrl":"https://doi.org/10.1155/2014/346809","url":null,"abstract":"We engineered a CSAD KO mouse to investigate the physiological roles of taurine. The disruption of the CSAD gene was verified by Southern, Northern, and Western blotting. HPLC indicated an 83% decrease of taurine concentration in the plasma of CSAD(-/-). Although CSAD(-/-) generation (G)1 and G2 survived, offspring from G2 CSAD(-/-) had low brain and liver taurine concentrations and most died within 24 hrs of birth. Taurine concentrations in G3 CSAD(-/-) born from G2 CSAD(-/-) treated with taurine in the drinking water were restored and survival rates of G3 CSAD(-/-) increased from 15% to 92%. The mRNA expression of CDO, ADO, and TauT was not different in CSAD(-/-) compared to WT and CSAD mRNA was not expressed in CSAD(-/-). Expression of Gpx 1 and 3 was increased significantly in CSAD(-/-) and restored to normal levels with taurine supplementation. Lactoferrin and the prolactin receptor were significantly decreased in CSAD(-/-). The prolactin receptor was restored with taurine supplementation. These data indicated that CSAD KO is a good model for studying the effects of taurine deficiency and its treatment with taurine supplementation. ","PeriodicalId":14901,"journal":{"name":"Journal of Amino Acids","volume":"2014 ","pages":"346809"},"PeriodicalIF":0.0,"publicationDate":"2014-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1155/2014/346809","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"32183500","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 64

Amino Acid compositions of 27 food fishes and their importance in clinical nutrition. 27种食用鱼的氨基酸组成及其临床营养意义。

Journal of Amino Acids Pub Date : 2014-01-01 Epub Date: 2014-10-14 DOI: 10.1155/2014/269797

Bimal Mohanty, Arabinda Mahanty, Satabdi Ganguly, T V Sankar, Kajal Chakraborty, Anandan Rangasamy, Baidyanath Paul, Debajit Sarma, Suseela Mathew, Kurukkan Kunnath Asha, Bijay Behera, Md Aftabuddin, Dipesh Debnath, P Vijayagopal, N Sridhar, M S Akhtar, Neetu Sahi, Tandrima Mitra, Sudeshna Banerjee, Prasenjit Paria, Debajeet Das, Pushpita Das, K K Vijayan, P T Laxmanan, A P Sharma

{"title":"Amino Acid compositions of 27 food fishes and their importance in clinical nutrition.","authors":"Bimal Mohanty, Arabinda Mahanty, Satabdi Ganguly, T V Sankar, Kajal Chakraborty, Anandan Rangasamy, Baidyanath Paul, Debajit Sarma, Suseela Mathew, Kurukkan Kunnath Asha, Bijay Behera, Md Aftabuddin, Dipesh Debnath, P Vijayagopal, N Sridhar, M S Akhtar, Neetu Sahi, Tandrima Mitra, Sudeshna Banerjee, Prasenjit Paria, Debajeet Das, Pushpita Das, K K Vijayan, P T Laxmanan, A P Sharma","doi":"10.1155/2014/269797","DOIUrl":"https://doi.org/10.1155/2014/269797","url":null,"abstract":"Proteins and amino acids are important biomolecules which regulate key metabolic pathways and serve as precursors for synthesis of biologically important substances; moreover, amino acids are building blocks of proteins. Fish is an important dietary source of quality animal proteins and amino acids and play important role in human nutrition. In the present investigation, crude protein content and amino acid compositions of important food fishes from different habitats have been studied. Crude protein content was determined by Kjeldahl method and amino acid composition was analyzed by high performance liquid chromatography and information on 27 food fishes was generated. The analysis showed that the cold water species are rich in lysine and aspartic acid, marine fishes in leucine, small indigenous fishes in histidine, and the carps and catfishes in glutamic acid and glycine. The enriched nutrition knowledge base would enhance the utility of fish as a source of quality animal proteins and amino acids and aid in their inclusion in dietary counseling and patient guidance for specific nutritional needs. ","PeriodicalId":14901,"journal":{"name":"Journal of Amino Acids","volume":"2014 ","pages":"269797"},"PeriodicalIF":0.0,"publicationDate":"2014-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1155/2014/269797","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"32799775","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 165