Japanese journal of bacteriology最新文献

{"title":"Studies on the Acetylenic Compounds","authors":"B. Nomiya, K. Kagino","doi":"10.3412/JSB.18.28","DOIUrl":"https://doi.org/10.3412/JSB.18.28","url":null,"abstract":"The authors found interesting results on acetylenic compounds by microbial and animal tests, which are described herein.It is known that same acetylenic compounds such as dicarbamoylacetylen (Collocidin: Lenamycin) and Capillin have antimicrobial activity. During the course of the synthetic study in this laboratory to find those acetylenic compounds with antimicrobial activity, we came to new compounds, halophenyl-γ-iodopropargyl ethers which have very potent activity on fungi and some other microbes. Among these compounds, 2, 4, 5-trichlorophenyl-γ-iodopropargyl ether had each minimal inhibitory concentration of 0.25γ/ml, 0.25γ/ml, 0.78γ/ml against Trichophyton asteroides, T. interdigitale, T. rubrum and also showed rather high activity for other fungi, Cryptococcus, Staphylococcus, Streptococcus, Bacillus subtilis, Mycobacterium in dilution assay.The high activity of the compound for Trichophyton in vitro was also proved by the curative effects ton infectious animal tests.(guinea-pig)","PeriodicalId":14812,"journal":{"name":"Japanese journal of bacteriology","volume":"90 1","pages":"28-37"},"PeriodicalIF":0.0,"publicationDate":"1963-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"80442610","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Fractionation of human strain Aoyama-B tuberculin by the use of Zone electrophoresis","authors":"T. Kataoka","doi":"10.3412/jsb.18.109","DOIUrl":"https://doi.org/10.3412/jsb.18.109","url":null,"abstract":"Two protein fractions II and III, obtained from human strain Aoyama-B tuberculin were tested on their sensitizing-activities comparing with DT and PPD-s.Four groups of guinea pigs were sensitized respectively by intramuscular injectionof fractions II, III, DT, or PPD-s mixed with Bayol F and Arlacel A. Thereafter animals were tested periodically on their skin reactivities with the intradermal injection of the same dose of fractions II, and III, DT, and PPD-s.Results were as follows:1) Animals were well sensitized in all groups and developed the maximal reactivity at 6th week after sensitization. The sensitizing-activity to induce the skin reaction to 5 mcg PPD-s in g-uinea pigs fell into the following sequence; DT, PPD-s, fraction II and fraction III.2) The animals sensitized with fraction II alone developed strong skin reaction to homologous antigen, fraction II.3) In the animals sensitized with DT or fraction II, the maximal reaction was observed at 24hrs. after intradermal injection of homologous antigen, respectively, but it was 8hrs. after injection with other antigens.","PeriodicalId":14812,"journal":{"name":"Japanese journal of bacteriology","volume":"11 1","pages":"109-114"},"PeriodicalIF":0.0,"publicationDate":"1963-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"74147621","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Transduction of Erythromycin-resistance in Staphylococcus","authors":"C. Niwa","doi":"10.3412/JSB.18.21","DOIUrl":"https://doi.org/10.3412/JSB.18.21","url":null,"abstract":"Using international typing phages 80 and 81, erythromycin-resistance was transduced from naturally resistant strains of Staph. aureus to erythromycin-sensitive strains of both Staph. aureus and Staph. epidermidis.160 strains of Staph. aureus which were highly sensitive to erythromycin were tested for the competence to serve as the recipients of erythromycin-resistance. All cases of transduction experiments revealed that about 10-17% of the tested strains were competent recipient, and all the transductants tested were resistant to 100mcg/ml of erythromycin. In 49 strains of Staph. epidermidis three or four strains were competent recipient, although the degree of resistance of the transductants were rather low compared with that of the appropriate donor.Some of the transductants transduced by the donor which was also highly resistant to oleandomycin received also the oleandomycin-resistance, while in the other transductants oleandomycin-resistance failed to be transmitted. Furthermore, joint transduction of penicillin- or streptomycin-resistance was observed in some of the transductants, but tetracycline-resistance of the donor was not jointly transduced.No change in the biological functions was detected in all of the transductants, and many of the transductants of Staph. aureus showed the same phage types as the original strains.","PeriodicalId":14812,"journal":{"name":"Japanese journal of bacteriology","volume":"111 3S 1","pages":"21-27"},"PeriodicalIF":0.0,"publicationDate":"1963-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"76165143","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Serological Properties of K-antigen of Vibrio parahemolyticus A-55 (Type 13)","authors":"G. Omori, M. Iwao","doi":"10.3412/JSB.18.379","DOIUrl":"https://doi.org/10.3412/JSB.18.379","url":null,"abstract":"An 0 agglutinable mutant was isolated from the ultraviolet-irradiated culture of Vibrio parahemolyticus. A-55 (Type 13). This mutant retained the flagellar H and somatic O antigen of its parent strain but lacked in a specific antigenic component which was considered as the K (Kapsel) antigen. The behavior against heat and HC1 of the K antigen, as revealed by agglutination and absorption tests and by antibody formation in the rabbit, resembled that of the B antigen found in certain ofE. coli.The O antigen of the mutant, naked of the K antigen, was destroyed by a treatment with HC1 resembling 5 antigen in certain of theSalmonella.","PeriodicalId":14812,"journal":{"name":"Japanese journal of bacteriology","volume":"1 1","pages":"379-383"},"PeriodicalIF":0.0,"publicationDate":"1963-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"90138607","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Vibrio El Torのアミノ酸要求について","authors":"Taketoshi Arai","doi":"10.3412/JSB.17.371","DOIUrl":"https://doi.org/10.3412/JSB.17.371","url":null,"abstract":"It was reported by Gott; and her coworkers (1959), that Vibrio El Tor strain Denken grow in a semisynthetic media consisting of Casamino acids, salts, glucose, vitamins and purine. The author confirmed that Casamino acids in this composition could be substituted with 18 pure amino acids mixture, and tried to simplify the amino acids composition by the elimination test. This strain could grow in a medium containing 8 amino acids, Methionine, Asparatic acid, Leucine, Phenylalanine, Isoleucine, Lysine, Arginine and Glutamic acid. Stain Denken showed moderate growth in this medium, a little less than in 1% Casamino acids medium or 18 amino acids mixture, and in this media 5 serial transfer was possible. Hemolysine production in this medium was approximately on the same level as in 1% Casamino acids medium.","PeriodicalId":14812,"journal":{"name":"Japanese journal of bacteriology","volume":"04 1","pages":"371-375"},"PeriodicalIF":0.0,"publicationDate":"1962-05-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"86293182","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Experimental Shigella Infections in Mice","authors":"K. Iwata, M. Yoshikawa, H. Motoe","doi":"10.3412/JSB.17.434","DOIUrl":"https://doi.org/10.3412/JSB.17.434","url":null,"abstract":"In a previous study the authors reported that Shigilla infection was experimentally induced in miceby oral inoculation of resistant strains of Shigella. This was accomplished by continuous administration ofantibiotics in combination with pre- and post-inoculation of resistance-lowering agents, such as carbon tetrachloride, which were very useful in the establishment of infection. In this connection the authors showedthat in such an infection, the presence of an increase number of antibiotic-resistant organisms, mainly Escherichia coli, due to the effect of chloramphenicol, disrupts the Shigella population.","PeriodicalId":14812,"journal":{"name":"Japanese journal of bacteriology","volume":"17 1","pages":"434-442"},"PeriodicalIF":0.0,"publicationDate":"1962-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"88544930","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Vi抗原およびSalmonella菌株と共通のO抗原を有するBacterium typhi-flavumの1菌株","authors":"Seizi Nakazaki","doi":"10.3412/JSB.17.38","DOIUrl":"https://doi.org/10.3412/JSB.17.38","url":null,"abstract":"A yellow pigment producing organism of Gram-negative, flagellated bacillus was isolated from the blood of a feverous patient. The serum of the patient taken on the fifth disease day showed comparatively high titer (1: 640) in the agglutination test with this organism. On isolation, this organism had possessed Vi-antigen, since the living and heated bacilli or this strain had been agglutinated by anti-Vi sera to full titer, but not by anti-S. typhi 0901 serum. However, on successive subculture on agar, the Vi-antigen was lost in 2 months after isolation and the organism showed reaction with anti-S. typhi 0901 serum. Contray to the first apperance that the organism seemed to be a strain of S. typhi, it was identified as Bact. typhiflavum by its culturai and biochemical properties (Cf. Cruickshank: J. Hyg., 1935, 35, 354). It formed so-called “Symplasmata” discribed by Kathe (1933) on agar slant, especially in the part of condensed water.As to the serological relationship between Bact. typhi-flavum and Salmonella groups, no satisfactory description could be found in the literature. Therefore, an attempt was made to investigate it using the isolated organism and more than 30 different Salmonella serotypes. After the many cross-absorption tests, it was found that this strain of Bact. typhi-flavum possessed such 0-antigens as Salmonella 0122, 13, and 34. Besides, it contained some partial 0-antigens common to such Salmonella serotypes as S. newington, S. albany, S. hoi-sham, S. minnesota and S. tel-aviv, respectively. By slide agglutination tests, the organism showed no reaction with any anti-H standard serum of Salmonella.On the cultivation in bile containg broth, the organism grew easily a variant strain producing no yellow pigment. Serologically this strain was identical to the original strain, but different in the fact that it did not ferment maltose and formed no symplasmata.Although the pathogenicity of the organism for mice was so weak, that on M. L. D. was about 7mg of culture by intraperitoneal inoculation, the present study, together with the literature survey, suggests that Bact. typhi-flavum would cause mild typhoid fever in man depending on conditions.","PeriodicalId":14812,"journal":{"name":"Japanese journal of bacteriology","volume":"108 1","pages":"38-47"},"PeriodicalIF":0.0,"publicationDate":"1962-01-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"75907233","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"On the Heatresistance of Cl. welchii","authors":"G. Morinaga, J. Yoshizawa","doi":"10.3412/JSB.17.899","DOIUrl":"https://doi.org/10.3412/JSB.17.899","url":null,"abstract":"","PeriodicalId":14812,"journal":{"name":"Japanese journal of bacteriology","volume":"72 1","pages":"899-902"},"PeriodicalIF":0.0,"publicationDate":"1962-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"86297829","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Studies on Non-Pathogenic Acid-Fast Bacilli","authors":"Y. Ochi, Heiji Sato","doi":"10.3412/JSB.16.1068","DOIUrl":"https://doi.org/10.3412/JSB.16.1068","url":null,"abstract":"The acid-fast bacilli were examined for the capacity of utilizing carbohydrates and organic acids as the carbon sources and sensitivity for antibiotics. The results obtained are asfollows.1. In general, most of the saprophytic strains were capable of utilizing more kinds of carbohydrates as carbon sources than the human or animal strains. The capacity ofutilizing carbohydrates, however, was of little value as criterion in classifying these acid-fast bacilli. Of the substances examined, monosaccharides and and sugar alcohol were utilized by most of the acid-fast strains as carbon sources. On the contrary, disaccharides and organic acids were utilized by only a few of the strains.2. Oxalate was utilized as carbon source only by a type of Mycobacterium smegmatis which is define as an organisms growing at 47°C and failing to survive heating at 60°C for one hour.3. Of 72 strains examined, all were resistant to 100γper ml of para-aminosalicylic acid and 66 to 100γper ml of isoniazed. Colored strains were more sensitive for streptomycin than colorless strains. There was a tendency that the strains which were intermediate betweenthe smooth and the rough type in colonial morphology were strongly resistant to streptomycin.","PeriodicalId":14812,"journal":{"name":"Japanese journal of bacteriology","volume":"26 1","pages":"1068-1072"},"PeriodicalIF":0.0,"publicationDate":"1962-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"76227526","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Studies on Staphylococcus","authors":"Kôsaku Yoshida, Y. Arai","doi":"10.3412/JSB.16.1042","DOIUrl":"https://doi.org/10.3412/JSB.16.1042","url":null,"abstract":"Immunoserological relationship between antigenic fractions extracted from Staphylococcus aureus and anti-Staphy. aureus rabbit serum were carried out by sensitized-blood cell haemagglutination reaction and anaphylactic contracting reaction of the isolated guinea pig's ileum passively sensitized with anti-Staphy. aureus rabbit serum. The results obtained were as follows: Antigenic fractions were obtained from Staphy. aureus (A-11) and Staphy. epidermidis (B-12) by the modified method of Verway. By chemical analysis Fraction-A-1 and A-2 was nucleoprotein, Fraction B was protein, Fraction C was polysaccharide and Fraction R was crude nucleoprotein. Coagulase was also prepared by Blobel's method. Tannic acid red cell haemaggulutination reaction was most sensitive when nucleoprotein was used as the sensitized antigen, next crude extraction, purified coagulase, protein and polysaccharide. Cross reactions were also observed with nucleoprotein, polysaccharide and protein derived from Staphy. epidermidis.Remarkable anaphylactic reaction of the isolated intestine of the guinea pig passively sensitized with anti-Staphy. aureus rabbit serum was observed by adding crude extract, protein-fraction or nucleoprotein fraction, but not a reaction was observed with polysaccharide or purified coagulase. However, intestinal anaphylactic reaction was also observed with crude extract and protein-fraction of Staphy. epidermidis. These facts suggest that common antigenicity exists in protein fraction of Staphy.aureus and Staphy. epidermidis.","PeriodicalId":14812,"journal":{"name":"Japanese journal of bacteriology","volume":"1 1","pages":"1042-1045"},"PeriodicalIF":0.0,"publicationDate":"1962-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"83146407","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}