关于Vibrio El Tor的氨基酸需求

Taketoshi Arai
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引用次数: 0

摘要

Gott;和她的同事(1959)发现,厄尔托弧菌菌株Denken在半合成培养基中生长,该培养基由酪胺酸、盐、葡萄糖、维生素和嘌呤组成。作者证实该组合物中的Casamino acids可以被18种纯氨基酸混合物取代,并试图通过消去试验来简化氨基酸组成。该菌株可在含有蛋氨酸、天冬氨酸、亮氨酸、苯丙氨酸、异亮氨酸、赖氨酸、精氨酸和谷氨酸8种氨基酸的培养基中生长。染色Denken在该培养基中表现出适度的生长,略低于1%的Casamino acids培养基或18个氨基酸混合物,并且在该培养基中可以进行5次连续转移。在这种培养基中,血红素的产量与在1%的酪胺酸培养基中大致相同。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Vibrio El Torのアミノ酸要求について
It was reported by Gott; and her coworkers (1959), that Vibrio El Tor strain Denken grow in a semisynthetic media consisting of Casamino acids, salts, glucose, vitamins and purine. The author confirmed that Casamino acids in this composition could be substituted with 18 pure amino acids mixture, and tried to simplify the amino acids composition by the elimination test. This strain could grow in a medium containing 8 amino acids, Methionine, Asparatic acid, Leucine, Phenylalanine, Isoleucine, Lysine, Arginine and Glutamic acid. Stain Denken showed moderate growth in this medium, a little less than in 1% Casamino acids medium or 18 amino acids mixture, and in this media 5 serial transfer was possible. Hemolysine production in this medium was approximately on the same level as in 1% Casamino acids medium.
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