Japanese journal of bacteriology最新文献_第7页

Studies on Corynebacterium diphtheriae Isolated in Taiwan 台湾白喉棒状杆菌分离株的研究

Japanese journal of bacteriology Pub Date : 1965-01-01 DOI: 10.3412/JSB.20.651

Wen-Fun Kuo

{"title":"Studies on Corynebacterium diphtheriae Isolated in Taiwan","authors":"Wen-Fun Kuo","doi":"10.3412/JSB.20.651","DOIUrl":"https://doi.org/10.3412/JSB.20.651","url":null,"abstract":"In order to analyse the mechanisms of host-parasite relationships in diphtherial infections, 4 strains of Corynebacterium diphtheriae isolated in Taiwan were first compared for their virulence by intradermal test in cortisone-treated and control (non-treated) groups of rabbits. It was found that #74 strain showed much weaker reaction as compared with other strains, i.e. #103, #78 and #69. This difference was apparent even after dilution of the inoculum to 10-1 or 10-2. In the dose-response curve, in which the amount of living bacilli (viable count) was taken into account, such difference in virulence was clearly observed. Therefore, the difference in virulence is not due to the difference in the number of viable bacilli present in each inoculum, but rather to the inherent ability of the individual bacillus itself to survive and produce toxin in the host.In the cortisone-treated groups of rabbits and guinea pigs, the skin reaction was generally markedly weaker than in control groups. The erythema at the site of injection was almost absent, and if present, it was observed to form a ring-form halo around the outer edge of induration. The induration was also less in size and thickness, and ulceration was rarely observed in the cortisone-treated groups.After intradermal injection of diphtheria bacilli, when the animals were not treated with a single injection of antitoxin to prevent premature death, the mortality of the infected rabbits and guinea pigs was not significantly different between the cortisone-treated and control groups. However, the positive rates of re-isolation of the diphtheria bacilli from regional lymph nodes and spleens of rabbits were significantly higher in cortisone treated than in control groups. Thus, the factors of parasite, i.e. inherent potency of the bacilli to survive and multiply in the host and toxin-producing ability, as well as the host factors such as amount of corticosterioids excreted, etc. seem to be the important determinants of diphtherial infection in man.","PeriodicalId":14812,"journal":{"name":"Japanese journal of bacteriology","volume":"26 1","pages":"651-657"},"PeriodicalIF":0.0,"publicationDate":"1965-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"75558492","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Studies on the Purification of Staphylococcal Coagulase and Its Properties 葡萄球菌凝固酶的纯化及其性质研究

Japanese journal of bacteriology Pub Date : 1965-01-01 DOI: 10.3412/JSB.20.627

H. Hitokoto

引用次数: 0

Immunochemical and Biochemical Studies of Fungi (4) 真菌免疫化学与生化研究(4)

Japanese journal of bacteriology Pub Date : 1965-01-01 DOI: 10.3412/JSB.20.693

O. Sakaguchi, Sigeo Suzuki, Masuko Suzuki, H. Sunayama

{"title":"Immunochemical and Biochemical Studies of Fungi (4)","authors":"O. Sakaguchi, Sigeo Suzuki, Masuko Suzuki, H. Sunayama","doi":"10.3412/JSB.20.693","DOIUrl":"https://doi.org/10.3412/JSB.20.693","url":null,"abstract":"From acetone-dried cells of C. albicans, we extracted the crude antigen with phosphate buffer solution (pH 7.2) containing phenol at concentrations of 45% at ordinary temperature (Fraction I), and from “cell. residue” with hot-water at 100 (Fraction II), and then further obtained a alkali soluble polysaccharide by use of 3% NaOH-extraction method under an atmosphere of N2 from insoluble “cell-residue” (Fraction III).The resulting crude extracts has been fractionated repeatedly with DEAE-Sephadex column, and a clarified Candida-mannan which showed a ultracentrifugically and electrophoretically single peak was obtained. In the fractions eluted from the column, there are two components of neutral and acidity fraction, and it has been ascertained that the later comprise a small amount of phosphorus.Each crude fractions of F. I, II, III showed nearly similar activities on precipitin reaction, and a decrease of their precipitin titer on account of drastic extraction methods was not observed.When Candida anti-serum was selectively absorbed with F. III-mannan, it was observed that the precipitin reaction of crude F. III expressed completely negative reaction and the precipitin titers were largely effected on their serological activities in the case of FI, II.","PeriodicalId":14812,"journal":{"name":"Japanese journal of bacteriology","volume":"12 1","pages":"693-699"},"PeriodicalIF":0.0,"publicationDate":"1965-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"76822028","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 2

Clostridium botulinumの毒素産生に関する実験的研究 (第1報) Clostridium botulinum毒素产生实验研究(第1报)

Japanese journal of bacteriology Pub Date : 1964-11-25 DOI: 10.3412/JSB.19.458

Hiroo Iida

引用次数: 7

[STUDIES ON THE BIOLOGICAL AND IMMUNOLOGICAL SIGNIFICANCE OF THE NUCLEIC ACIDS OF ENTERITIS-CAUSING VIBRIO. 1. THE PURIFICATION AND CHEMICAL PROPERTIES OF THE NUCLEAR AND TOXIC PORTION OF THE ENTERITIS-CAUSING VIBRIO]. 致肠炎弧菌核酸的生物学和免疫学意义的研究。1. 引起肠炎的弧菌的核和毒性部分的纯化和化学性质。

Japanese journal of bacteriology Pub Date : 1964-10-01 DOI: 10.3412/JSB.19.418

K. Kuriyama

引用次数: 0

Studies on the Immunospecific and Non-specific Substance of Bacillus anthracis 炭疽芽孢杆菌免疫特异性和非特异性物质的研究

Japanese journal of bacteriology Pub Date : 1964-01-01 DOI: 10.3412/JSB.18.455

T. Baba

引用次数: 0

ELECTRON MICROSCOPE STUDIES ON THE STRUCTURES OF PATHOGENIC FUNGI. I. ON THE STRUCTURE OF ULTRATHIN SECTIONS OF CANDIDA ALBICANS 病原菌结构的电镜研究。1 .白色念珠菌超薄切片的结构

Japanese journal of bacteriology Pub Date : 1963-11-01 DOI: 10.3412/JSB.18.393

K. Iwata, T. Hirata

{"title":"ELECTRON MICROSCOPE STUDIES ON THE STRUCTURES OF PATHOGENIC FUNGI. I. ON THE STRUCTURE OF ULTRATHIN SECTIONS OF CANDIDA ALBICANS","authors":"K. Iwata, T. Hirata","doi":"10.3412/JSB.18.393","DOIUrl":"https://doi.org/10.3412/JSB.18.393","url":null,"abstract":"By means of ultrathin section and electron microscopic technic the fine structures ofCandida albicansin yeast phase grown on Sabouraud's dextrose agar were revealed. Fixation of specimens proved to be superior in revealing the fine structures using a 2 % potassium permanganate aqueous solution (at room temperature for 48 hours) than with an osmic acid solution. Embedding was done in a routine method. The results obtained were as follows:The cell wall, relatively thick, consists of the three layers, two electron less dense ones divided by a dense interspace. The cytoplasmic membrane, very thin, is recognized.The nuclear apparatus is surrounded by a continous double-membraneous layer with pores; The nucleolus-like structures are observed as an electron dense region.The mitochondria with cristae mitochondriales are also surrounded by a continous double-membraneous layer as in the nucleus.The endoplasmic reticulum seems to be connected with the outer layers of both nucleus and mitochondria. The structures resembling the lamella, potassium permanganophilic uniform granules and vacuoles are also observed inside the cytoplasm.","PeriodicalId":14812,"journal":{"name":"Japanese journal of bacteriology","volume":"52 1","pages":"393-404"},"PeriodicalIF":0.0,"publicationDate":"1963-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"86685787","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 4

新らしいジフテリア菌分離培地-HB培地-の研究新的白喉菌分离培养基-HB培养基的研究

Japanese journal of bacteriology Pub Date : 1963-08-25 DOI: 10.3412/JSB.18.362

Y. Kaneko, F. Moriwaki, Riichi Sakazaki, Shoji Enomoto

引用次数: 0

Studies on the Nutritional Requirements of Bacteria XIII 细菌营养需求的研究[j]

Japanese journal of bacteriology Pub Date : 1963-01-01 DOI: 10.3412/JSB.18.375

E. Takemori, D. Mizuno

引用次数: 0

Relationship between the Intracellular Multiplication of Salmonellae in Cultured Mononuclear Phagocytes and their Pathogenicity for the Host 培养单核吞噬细胞中沙门氏菌胞内增殖与宿主致病性的关系

Japanese journal of bacteriology Pub Date : 1963-01-01 DOI: 10.3412/JSB.18.38

D. Ushiba, Kazuhisa Saito, M. Nakano, T. Akiyama, Y. Kishimoto, F. Okitsu, T. Saito

{"title":"Relationship between the Intracellular Multiplication of Salmonellae in Cultured Mononuclear Phagocytes and their Pathogenicity for the Host","authors":"D. Ushiba, Kazuhisa Saito, M. Nakano, T. Akiyama, Y. Kishimoto, F. Okitsu, T. Saito","doi":"10.3412/JSB.18.38","DOIUrl":"https://doi.org/10.3412/JSB.18.38","url":null,"abstract":"The intracellular growth of various species of salmonellae was tested by the roller tube technique using cultured peritoneal macrophages from DK1 strain of mice which were selectively inbred by a uniform susceptibility to S. enteritidis infection. Intracellular growth was observed markedly with S. enteritidis, S. typhimurium and S. choleraesuis, but not at all with S. paratyphi A, S. typhosa (V and VW types), and also with Escherichia coli (0-55 type). Some strains of S. paratyphi B showed a slight intracellular growth when large numbers of phagocytes were examined. When those strains of S. paratyphi B were intraperitoneally inoculated into DK1 mice, large numbers of organisms were harbored in the peritoneal fluid and organs through the 3rd week compared with other strains of the same species and S. paratyphi A or S. typhosa. Histopathological findings of the liver of inoculated mice showed the formation of granulomas or typhoms, large and small, in the degree almost parallel to the number of harbored organisms. The coincidence between the apparent intracellular growth of bacterial species and their capability to produce typhoid infection in mice was emphasized.","PeriodicalId":14812,"journal":{"name":"Japanese journal of bacteriology","volume":"18 13 1","pages":"38-43"},"PeriodicalIF":0.0,"publicationDate":"1963-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"79822967","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1