Immunochemical and Biochemical Studies of Fungi (4)

From acetone-dried cells of C. albicans, we extracted the crude antigen with phosphate buffer solution (pH 7.2) containing phenol at concentrations of 45% at ordinary temperature (Fraction I), and from “cell. residue” with hot-water at 100 (Fraction II), and then further obtained a alkali soluble polysaccharide by use of 3% NaOH-extraction method under an atmosphere of N2 from insoluble “cell-residue” (Fraction III).The resulting crude extracts has been fractionated repeatedly with DEAE-Sephadex column, and a clarified Candida-mannan which showed a ultracentrifugically and electrophoretically single peak was obtained. In the fractions eluted from the column, there are two components of neutral and acidity fraction, and it has been ascertained that the later comprise a small amount of phosphorus.Each crude fractions of F. I, II, III showed nearly similar activities on precipitin reaction, and a decrease of their precipitin titer on account of drastic extraction methods was not observed.When Candida anti-serum was selectively absorbed with F. III-mannan, it was observed that the precipitin reaction of crude F. III expressed completely negative reaction and the precipitin titers were largely effected on their serological activities in the case of FI, II.