Studies on Staphylococcus

Japanese journal of bacteriology Pub Date : 1962-01-01 DOI:10.3412/JSB.16.1042

Kôsaku Yoshida, Y. Arai

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Abstract

Immunoserological relationship between antigenic fractions extracted from Staphylococcus aureus and anti-Staphy. aureus rabbit serum were carried out by sensitized-blood cell haemagglutination reaction and anaphylactic contracting reaction of the isolated guinea pig's ileum passively sensitized with anti-Staphy. aureus rabbit serum. The results obtained were as follows: Antigenic fractions were obtained from Staphy. aureus (A-11) and Staphy. epidermidis (B-12) by the modified method of Verway. By chemical analysis Fraction-A-1 and A-2 was nucleoprotein, Fraction B was protein, Fraction C was polysaccharide and Fraction R was crude nucleoprotein. Coagulase was also prepared by Blobel's method. Tannic acid red cell haemaggulutination reaction was most sensitive when nucleoprotein was used as the sensitized antigen, next crude extraction, purified coagulase, protein and polysaccharide. Cross reactions were also observed with nucleoprotein, polysaccharide and protein derived from Staphy. epidermidis.Remarkable anaphylactic reaction of the isolated intestine of the guinea pig passively sensitized with anti-Staphy. aureus rabbit serum was observed by adding crude extract, protein-fraction or nucleoprotein fraction, but not a reaction was observed with polysaccharide or purified coagulase. However, intestinal anaphylactic reaction was also observed with crude extract and protein-fraction of Staphy. epidermidis. These facts suggest that common antigenicity exists in protein fraction of Staphy.aureus and Staphy. epidermidis.

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葡萄球菌的研究

金黄色葡萄球菌抗原提取物与抗葡萄球菌的免疫血清学关系。采用豚鼠离体回肠被动致敏后的致敏血细胞血细胞凝集反应和过敏收缩反应检测金黄色葡萄球菌兔血清。金黄色兔子血清。结果如下:从Staphy中获得抗原部分。aureus (A-11)和Staphy。通过改良的Verway方法提取表皮细胞(B-12)。经化学分析，a -1和A-2组分为核蛋白，B组分为蛋白质，C组分为多糖，R组分为粗核蛋白。用Blobel法制备凝固酶。以核蛋白为致敏抗原时单宁酸红细胞血凝反应最敏感，其次为粗提物、纯化凝固酶、蛋白和多糖。与核蛋白、多糖及葡萄球菌蛋白均有交叉反应。epidermidis。抗葡萄球菌药被动致敏豚鼠离体肠有明显的过敏反应。用粗提物、蛋白部分或核蛋白部分对金黄色葡萄球菌血清进行观察，但与多糖或纯化凝固酶均无反应。然而，用葡萄树粗提物和蛋白部分也观察到肠道过敏反应。epidermidis。这些事实表明葡萄球菌蛋白部分存在共同的抗原性。aureus and Staphy。epidermidis。

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Japanese journal of bacteriology

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