Journal fur Hirnforschung最新文献

{"title":"Cornu Ammonis of the dog: a rudimentary CA2 field is only present in a small part of the dorsal division, and is absent in the ventral division of the cornu Ammonis.","authors":"H Amayasu,&nbsp;K Shoumura,&nbsp;N Ichinohe,&nbsp;S Yu,&nbsp;H Yonekura","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>The relationship between mossy fiber projections and cytoarchitecture was studied in the cornu Ammonis (CA) of three mongrel dogs and two Japanese white rabbits. Precise comparisons were accomplished by examining adjoining sections that were stained by two methods: 1) the Timm-Danscher method which stains for zinc, which is contained in high concentrations in the mossy fiber terminals, and 2) thionin staining of the Nissl substance, so that the mossy fiber projections and cytoarchitecture could be closely compared in each subdivision of the CA. There was a cytoarchitectonically transitional zone (TZ) between CA3 and CA1 in both dogs and rabbits. The TZ was composed of an intermingling of CA3-like large pyramidal cells and CA1-like small pyramidal cells. Across the dorso-ventral length of the rabbit CA, the mossy fiber projections emitted from the dentate granular cell layer extended as far as the distal border of CA3 toward the TZ but did not enter the TZ or CA1. The TZ of the rabbit was equivalent to CA2 of Lorente de Nó (1934), that was characterized by CA3-like large pyramidal cells which did not receive mossy fiber inputs. In the dorsal CA of the dog, the mossy fiber projections extended across the CA3/TZ border, as far as the most distal part of the TZ in one dog but only for a short distance in the other two dogs. That part of the TZ which did not receive mossy fiber projections corresponded with CA2. There were no mossy fiber projections to CA1. In the ventral CA of the dog, however, mossy fiber projections were more extensive than in the dorsal CA, extending across CA3/TZ as well as TZ/CA1 borders to enter the proximal part of CA1. These observations indicate that there is a distinct CA2 in the rabbit CA, and that a rudimentary CA2 is present only in a small part of the dorsal CA of the dog, whereas it is absent in the ventral CA.</p>","PeriodicalId":14790,"journal":{"name":"Journal fur Hirnforschung","volume":"39 3","pages":"355-67"},"PeriodicalIF":0.0,"publicationDate":"1999-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"21397984","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"PCNA positivity in the telencephalic matrix areas in the adult of a lizard, Podarcis sicula.","authors":"V Margotta,&nbsp;A Morelli,&nbsp;L Alfei","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>In the present paper a reappraisal is made of the persistence and size of telencephalic matrix areas in normal adult specimens of a lizard, Podarcis sicula, using a recent in situ marker of cells in the S phase, the Proliferating Cell Nuclear Antigen (PCNA), belonging to the cyclin family, proteins that regulate the cell cycle and that can be immunocytochemically evidenced using monoclonal antibodies. Only a small number of positive PCNA elements, randomly arranged in the dorsal (medial and lateral) matrix areas, were found in the telencephalic ependymal tissue. These elements were found in larger numbers and distributed more uniformly in the ventral matrix areas, both opposite the ventricular lumen and outside the ependyma.</p>","PeriodicalId":14790,"journal":{"name":"Journal fur Hirnforschung","volume":"39 3","pages":"271-6"},"PeriodicalIF":0.0,"publicationDate":"1999-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"21397468","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Ultrastructural changes in the sinuatrial and atrioventricular nodes of the heart of the monkey (Macaca fascicularis) after bilateral vagotomy.","authors":"M M Aye,&nbsp;G Singh,&nbsp;S S Tay","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>This study describes the ultrastructural changes in the sinu-atrial (SA) and atrio-ventricular (AV) nodes of the monkey (Macaca fascicularis) after bilateral mid-cervical vagotomy at 1, 3, 5, 7 and 14 days post-operations. The changes were similar in both types of nodal cells. The most obvious feature of the degenerating nodal cells was the swollen mitochondria with disrupted cristae. Other changes include increased granular sarcoplasmic reticulum, increased glycogen particles, vacuolation of mitochondria and increased lysosomal activity. Axonal profiles in the vicinity of the nodal cells showed swelling and vacuolation. Cardiac neurons also showed some changes such as distended granular endoplasmic reticulum, increased accumulation of glycogen particles and increased lipofuscin granules. Macrophages and Schwann cells were the main scavengers in removing the degenerated nodal cells and axonal profiles. In the case of affected cardiac neurons, satellite cells seemed to act as main scavenger cells. It is postulated that the nodal cells are dependent on the incoming fibres of the vagus nerve for their survival. By an understanding of the ultrastructural changes in the nodal cells after bilateral vagotomy, it may help in developing new strategies to explore in depth of the conducting system of the heart.</p>","PeriodicalId":14790,"journal":{"name":"Journal fur Hirnforschung","volume":"39 4","pages":"441-7"},"PeriodicalIF":0.0,"publicationDate":"1999-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"21686403","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The brain lesion influences the numbers of peripheral blood leukocytes in the rat.","authors":"P Zach,&nbsp;P Kruzík,&nbsp;K Smetana,&nbsp;J Kvasnicka,&nbsp;P Petrovicky","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>The mutual relationship between the central nervous and immune system are intensively studied. The lesion of distinct structures of the rat brain such as septum influence the model immune response such as lymphocyte proliferation and delayed skin hypersensitivity. Employing the model of the damage of septum in the rat brain by electrolesion we demonstrated the decrease of the number of peripheral blood leukocytes, mainly cells exhibiting CD25 and CD45RA antigens in the rat. The striatum destruction has much lower influence on the studied parameters, which suggests a specific effect of the septum on these hematological parameters.</p>","PeriodicalId":14790,"journal":{"name":"Journal fur Hirnforschung","volume":"39 4","pages":"489-92"},"PeriodicalIF":0.0,"publicationDate":"1999-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"21686409","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"5-HT potentiates GABA- and glycine-activated chloride currents on the same neurons in rat spinal cord.","authors":"D S Wang,&nbsp;T L Xu,&nbsp;J S Li","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>The electrophysiological and pharmacological properties of GABA- and glycine (Gly)-induced responses were investigated in neurons acutely dissociated from the rat sacral dorsal commissural nucleus (SDCN) using the nystatin perforated patch recording configuration under voltage-clamp conditions. At a holding potential of -40 mV, the currents evoked by GABA and Gly increased in a sigmoidal fashion with increasing agonist concentration. The reversal potentials of I(GABA)and I(Gly) were close to the Cl- equilibrium potential. Bicuculline (BIC) and strychnine (STR) suppressed I(GABA) and I(Gly) in a concentration-dependent manner, although they have different affinities for GABA and Gly. 5-HT potentiated both I(GABA) and I(Gly) via intracellular protein kinase C on the same neurons. The results indicated that the acutely dissociated SDCN neurons responded to both exogenous GABA and Gly, which activated GABA(A) and STR-sensitive Gly receptors, respectively, and 5-HT may produce spinal antinociception through postsynaptic potentiation of I(GABA) and I(Gly) when they are coreleased; GABA and Gly may act as cotransmitters implicated in the control of spinal nociceptive signal processing in the mammalian spinal cord.</p>","PeriodicalId":14790,"journal":{"name":"Journal fur Hirnforschung","volume":"39 4","pages":"531-7"},"PeriodicalIF":0.0,"publicationDate":"1999-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"21686999","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Neuronal connections through the posterior commissure in the frog Rana esculenta.","authors":"G Lázár,&nbsp;E Pál","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>The cobalt-labelling technique was used. After iontophoretic injections of cobaltic-lysine complex into the posterior commissure, fibres and neurons were bilaterally labelled in the posterior thalamic nucleus, three other pretectal nuclei, the optic tectum, the nucleus of the medial longitudinal fasciculus, and in the basal optic nucleus. Unilateral cobalt injections into the terminal areas of the posterior commissure in the mesencephalic tegmentum, labelled neurons in the above-mentioned nuclei ipsilaterally, and some cells contralaterally. The connections through the frog's posterior commissure are very similar to those in mammals.</p>","PeriodicalId":14790,"journal":{"name":"Journal fur Hirnforschung","volume":"39 3","pages":"369-74"},"PeriodicalIF":0.0,"publicationDate":"1999-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"21397905","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Expression and distribution of various antigens of developing microglial cells in the rat telencephalon.","authors":"B Domaradzka-Pytel,&nbsp;B Ludkiewicz,&nbsp;J Moryś,&nbsp;H M Wisniewski","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>The distribution of microglia during the early stages of postnatal development in the rat was studied on rat brain from day of birth to postnatal day 90 (P90), using immunohistochemical methods with a panel of monoclonal antibodies that recognized the complement type 3 receptor (OX-42), macrophage antigen of unknown function (ED1), and the major histocompatibility complex (MHC) class I (OX-18) or class II (OX-6) antigens. Starting from the day of birth, ameboid microglia can be differentiated with positive immunoreactivity to OX-42, OX-18, and ED1. Labeled cells were localized mainly in the developing white matter. After P21, only positive reaction to OX-42 was present, and those cells had the typical morphology of the resting microglial cells that were located either in the white or grey matter. The changes in the appearance of different antigens are correlated with the morphological differentiation and transformation of ameboid microglial cells that are to become ramified microglia, present in the adult animals.</p>","PeriodicalId":14790,"journal":{"name":"Journal fur Hirnforschung","volume":"39 3","pages":"283-91"},"PeriodicalIF":0.0,"publicationDate":"1999-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"21397977","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Projections from subnucleus oralis of the spinal trigeminal nucleus to contralateral thalamus via the relay of juxtatrigeminal nucleus and dorsomedial part of the principal sensory trigeminal nucleus in the rat.","authors":"J D Zhang,&nbsp;X L Yang","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>Following injection of HRP into contralateral thalamus, retrogradely labeled cells were observed in principal sensory trigeminal nucleus (Vp) and an area of juxtatrigeminal nucleus (JX) formerly described by John and Tracey (1987). When PHA-L was delivered to dorsomedial part of the subnucleus oralis (Vodm), PHA-L labeled terminals were seen in dorsomedial part of the Vp (Vpdm) and in the JX region. Comparing the distribution of PHA-L labeled terminal field with that of HRP labeled JX neurons showed that the labeled terminals and neurons were overlapped closely in the JX. The distribution patterns of the labeled terminals and JX neurons were also the same: viewed on the coronal planes caudal-rostrally, both of the labelings began to appear at the levels where the facial nerve root was just broken. Rostrally, at middle levels of the motor trigeminal nucleus (Vmo), the labelings showed their typical view covering dorsal and ventral JX (dJX, vJX). The labelings disappeared at rostral poles of the Vmo and Vp. When injections of PHA-L into the Vodm and HRP into the contralateral thalamus was made in one rat, the contacts between Vodm projecting terminals labeled with PHA-L and HRP labeled trigemino-thalamic neurons were seen in the JX and also in the Vpdm. Then, electron microscopic (EM) study was done, injections of kainic acid into the Vodm and HRP into the contralateral thalamus was performed simultaneously. After EM embedding, the JX and Vpdm regions were selected, ultrathin sections were cut and observed with EM. In both areas, axo-somatic and axo-dendritic synapses were seen between degenerated boutons and HRP labeled somata or dendrites. Namely, the Vodm projecting terminals synapsed on trigemino-thalamic neurons in the JX and Vpdm. Anyway, axo-dendritic synapses was the main type of observed synapses. Thus, the present work demonstrated 1. the JX containing a group of trigemno-thalamic neurons was a target of special projections froin the Vodm; 2. The Vodm neurons projected to the contralateral thalamus through the relay of JX and Vpdm neurons.</p>","PeriodicalId":14790,"journal":{"name":"Journal fur Hirnforschung","volume":"39 3","pages":"301-10"},"PeriodicalIF":0.0,"publicationDate":"1999-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"21397979","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Formation of the corticopontine projection in a long-term culture system.","authors":"D Echevarría,&nbsp;K Albus","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>The reestablishment in vitro of the corticopontine projection was studied in organotypic co-cultures of cortex and pons of rats 0 (day of birth) - 3 days old. After 2-3 weeks in vitro, application of the lipophilic tracer DiI in the pontine explant retrogradely stained in layer V of the cortical explant pyramidal neurons which were characterized by large somata and spiny dendrites with an apical dendrite that reached upper cortical layers II/III. The projection developed only in co-cultures from rats 0-2 days old when the pontine explant was placed in close vicinity either to the white matter or the pial surface of the cortical explant. Control experiments demonstrated the specificity of the corticopontine projection in vitro by showing that the projections of the layer V pyramidal cells to the pons did not reflect a non-directed outgrowth pattern with subsequent survival of axons contacting the target explant. Our findings demonstrate that morphology and laminar position of corticopontine projection neurons in vitro are similar to those in vivo and thus support the \"organotypic\" nature of the explant co-culture system.</p>","PeriodicalId":14790,"journal":{"name":"Journal fur Hirnforschung","volume":"39 3","pages":"293-9"},"PeriodicalIF":0.0,"publicationDate":"1999-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"21397978","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Fine structure, synaptology and immunocytochemistry of large neurons in the rat dorsal cochlear nucleus connected to the inferior colliculus.","authors":"L Alibardi","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>Neurons in the rat dorsal cochlear nucleus that project to the inferior colliculus (pyramidal and giant) were retrograde labelled with wheat germ agglutinin conjugated to horseradish peroxydase. Both cell types showed a similar ultrastructural feature, particularly the rough endoplasmic reticulum was well developed and sometimes surrounded the nucleus. The synaptological profile was similar in pyramidal and giant cells. Axo-somatic terminals covered 40-70% of the perimeter of pyramidal cells and 35-60% of the perimeter of giant neurons. Giant neurons featured bipolar or multipolar shape and different orientation but they possessed a similar synaptic profile. Most axo-somatic terminals contained flat and pleomorphic synaptic vesicles, some pleomorphic vesicles. Few terminals contained round vesicles. These cells were consistently immuno-negative for both glycine and GABA and variably positive for glutamate. The immunoelectron microcopic study of thin sections showed that glycine immunoreactivity was constantly present in terminals enriched with flat vesicles, which often did not show GABA immunoreactivity. Few anterograde labelled boutons containing flat vesicles were in contact with the proximal dendrites and the cell bodies of pyramidal and giant neurons. The origin of these terminals is discussed. No other cells of the dorsal cochlear nucleus, in particular cartwheel and tuberculo-ventral neurons, were in contact with labelled boutons. The present results suggest that descending inhibitory collicular projections are essentially directed to the large excitatory neurons of the dorsal cochlear nucleus.</p>","PeriodicalId":14790,"journal":{"name":"Journal fur Hirnforschung","volume":"39 4","pages":"429-39"},"PeriodicalIF":0.0,"publicationDate":"1999-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"21686402","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}