Israel Journal of Chemistry最新文献_第2页

Discovery and Accurate Detection of Rare Nucleic Acid Modifications 发现并准确检测罕见的核酸修饰

IF 2.3 4区化学

Israel Journal of Chemistry Pub Date : 2024-06-24 DOI: 10.1002/ijch.202400024

Ru-Jia Luo, Hong-Xuan Chen, Jin-Wen Kong, Zhang Zhang, Nabieh Ayoub, Guan-Zheng Luo

引用次数: 0

Expanding the Scope of Ribosome-Mediated Biosynthesis in vitro using tRNA-Aminoacylating Ribozyme 利用 tRNA 氨基酰化核糖体酶扩大核糖体介导的体外生物合成的范围

IF 2.3 4区化学

Israel Journal of Chemistry Pub Date : 2024-06-17 DOI: 10.1002/ijch.202300174

Namjin Cho, Haneul Jin, Hyewon Jeon, Kanghun Lee, Joongoo Lee

引用次数: 0

Anionic Calixarenes in Biomembrane Transport of Peptides 多肽生物膜传输中的阴离子钙钛矿

IF 2.3 4区化学

Israel Journal of Chemistry Pub Date : 2024-06-17 DOI: 10.1002/ijch.202400023

Justin Neumann, Andreas Hennig

{"title":"Anionic Calixarenes in Biomembrane Transport of Peptides","authors":"Justin Neumann, Andreas Hennig","doi":"10.1002/ijch.202400023","DOIUrl":"https://doi.org/10.1002/ijch.202400023","url":null,"abstract":"Biomembranes function as hydrophobic barriers for hydrophilic substances enabling compartmentalization in biological systems. This poses, however, a problem for the targeted introduction of cargo into cells. The result is a high demand for delivery pathways into cells with the goal to investigate biological processes or to treat diseases by improved delivery. Polycationic cell-penetrating peptides (CPPs) are interesting as they can cross cell membranes and transport attached cargos directly into the cytosol. Their efficiency can be improved by anionic amphiphilic counterion activators, which bind to the CPPs to form charge-neutralized counterion-CPP complexes with sufficient hydrophobicity to cross the lipid bilayer membrane. This review summarizes recent results, which establish amphiphilic calixarenes as a new class of biocompatible and non-cytotoxic counterion activators with very high transport activities at nanomolar concentrations. We also include a brief summary of fluorescence-based assays with large unilamellar vesicles (LUVs) to investigate counterion-activated transport. Current methods use liposome-encapsulated, supramolecular host-dye reporter pairs including calixarenes, which provide new mechanistic insights and enable rapid in vitro identification of suitable activators. Taken together, amphiphilic calixarenes are currently emerging as prime candidates for counterion activation of membrane transport, which are highly modifiable and can be specifically tailored towards different cargoes and membrane types.","PeriodicalId":14686,"journal":{"name":"Israel Journal of Chemistry","volume":null,"pages":null},"PeriodicalIF":2.3,"publicationDate":"2024-06-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/ijch.202400023","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141967954","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Practical Applications of Supramolecular Macrocyclic Hosts in the Field of Herbal Medicines 超分子大环宿主在中草药领域的实际应用

IF 2.3 4区化学

Israel Journal of Chemistry Pub Date : 2024-06-14 DOI: 10.1002/ijch.202300179

Huijuan Yu, Kejing Niu, Yuting Zhao, Yuefei Wang

{"title":"Practical Applications of Supramolecular Macrocyclic Hosts in the Field of Herbal Medicines","authors":"Huijuan Yu, Kejing Niu, Yuting Zhao, Yuefei Wang","doi":"10.1002/ijch.202300179","DOIUrl":"10.1002/ijch.202300179","url":null,"abstract":"Herbal medicines (HMs) are gaining increasing popularity and recognition worldwide due to their eco-friendliness and efficacy. With their multi-compounds, multi-targets, and multi-pathways characteristics, HMs have been used in treatment of various diseases. However, the clinical applications of preparations containing HMs have been limited due to their inherent physicochemical properties, including low water solubility, poor stability, and unsatisfactory bioavailability of bioactive compounds. Supramolecular macrocyclic hosts, like cyclodextrins, calixarenes, cucurbiturils, and pillararenes, are important objects of researches in supramolecular chemistry. These hosts have been utilized to encapsulate the ingredients, improve the solubility of poorly water-soluble components, enhance the stability of the tested compounds, increase the bioavailability of bioactive compounds, and ensure the safety of HMs. Herein, we provide a brief introduction to the theories of supramolecular chemistry and summarize the extensive applications of supramolecular macrocyclic hosts in the field of HMs. These applications encompass the screening of bioactive compounds in HMs and the enhancement of druggability for HMs. We hope this review can provide a strategy for dealing with the challenges of HMs, thereby enabling their better applications and development.","PeriodicalId":14686,"journal":{"name":"Israel Journal of Chemistry","volume":null,"pages":null},"PeriodicalIF":2.3,"publicationDate":"2024-06-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141343353","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The Great Codon Escape: Vacating Codons for Genetic Code Expansion and Ribosome Stalling 密码子大逃亡空缺密码子促进遗传密码扩展和核糖体停滞

IF 2.3 4区化学

Israel Journal of Chemistry Pub Date : 2024-06-12 DOI: 10.1002/ijch.202400012

Antonius J. P. Hopstaken, Enno Große Wichtrup, Dr. Seino A. K. Jongkees

{"title":"The Great Codon Escape: Vacating Codons for Genetic Code Expansion and Ribosome Stalling","authors":"Antonius J. P. Hopstaken, Enno Große Wichtrup, Dr. Seino A. K. Jongkees","doi":"10.1002/ijch.202400012","DOIUrl":"10.1002/ijch.202400012","url":null,"abstract":"In ribosomal synthesis of peptides and proteins, genetic information is translated into an amino acid polymer according to the genetic code, which describes the translational command encoded by each codon. However, parts of the genetic code can be adjusted to customize translations. One option is to remove decoding for a specific codon, resulting in a vacant codon. Such vacant codons can be used to stall the ribosome for mechanistic studies and display techniques. Alternatively, the liberated codon can be assigned to encode for incorporation of a noncanonical building block for expansion of the genetic code. In this review we provide an overview of the methods currently available for vacating codons in prokaryotic translation (agnostic of how these are later applied), targeting factors such as amino-acyl tRNA synthetases, tRNA, release factors, and the initiation machinery. Moreover, we assess applicability and compatibility of the currently available techniques and discuss which have the potential to develop into even more powerful approaches in the future.","PeriodicalId":14686,"journal":{"name":"Israel Journal of Chemistry","volume":null,"pages":null},"PeriodicalIF":2.3,"publicationDate":"2024-06-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/ijch.202400012","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141352039","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Supramolecular Approaches to the Detoxification of Nerve Agents 神经毒剂的超分子解毒方法

IF 2.3 4区化学

Israel Journal of Chemistry Pub Date : 2024-06-11 DOI: 10.1002/ijch.202400019

Prof. Dr. Stefan Kubik

引用次数: 0

Spatially and Single-Cell Resolved Profiling of RNA Life Cycle and Epitranscriptomics 以空间和单细胞分辨率剖析 RNA 生命周期和外显子转录组学

IF 2.3 4区化学

Israel Journal of Chemistry Pub Date : 2024-06-03 DOI: 10.1002/ijch.202400028

Qiyang Zhou, Jianting Guo, Xiao Wang

{"title":"Spatially and Single-Cell Resolved Profiling of RNA Life Cycle and Epitranscriptomics","authors":"Qiyang Zhou, Jianting Guo, Xiao Wang","doi":"10.1002/ijch.202400028","DOIUrl":"10.1002/ijch.202400028","url":null,"abstract":"The intricate network of cell functions relies on gene expression programs, where the whole RNA life cycle from DNA to protein is subjected to extensive transcriptional and post-transcriptional gene regulation events. Established bulk RNA sequencing methods provide an averaged, transcriptome-wide quantification of the RNA life cycle, including transcription, processing, translation, transport, and degradation through RNA-protein interactions. Furthermore, numerous studies using bulk epitranscriptomic profiling unveiled that dynamic RNA modifications (e. g., N6-Methyladenosine), add another layer of gene regulations. However, many regulatory events are cell-type specific, subcellularly localized, and subjected to cell-cell communications within the native tissue environment. Thanks to the advances in single-cell sequencing, spatial sequencing, and highly multiplexed imaging methods, we can routinely measure single-cell and spatial transcriptomics. Yet more comprehensive methods to profile every step of the RNA life cycle with single-cell and spatial information are still lacking. In this review, we will summarize and compare early explorations in developing state-of-the-art methods for spatially and single-cell resolved mapping of RNA kinetics, translation, RNA-protein interactions, and epitranscriptomics. It is promising that these new techniques will greatly facilitate our understanding of the RNA-centered regulation landscapes in different cell types and how the post-transcriptional regulations are interconnected within cellular and tissue architecture.","PeriodicalId":14686,"journal":{"name":"Israel Journal of Chemistry","volume":null,"pages":null},"PeriodicalIF":2.3,"publicationDate":"2024-06-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/ijch.202400028","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141270198","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Direct, Quantitative, and Base-Resolution Sequencing of DNA and RNA Modifications 对 DNA 和 RNA 修饰进行直接、定量和碱基分辨率测序

IF 2.3 4区化学

Israel Journal of Chemistry Pub Date : 2024-05-31 DOI: 10.1002/ijch.202400007

Haiqi Xu, Chun-Xiao Song

引用次数: 0

Ethnic Variation and Structure‐Function Analysis of Tauopathy‐Associated PERK Alleles 与 Tauopathy 相关的 PERK 等位基因的种族差异和结构功能分析

IF 3.2 4区化学

Israel Journal of Chemistry Pub Date : 2024-05-24 DOI: 10.1002/ijch.202300173

Goonho Park, Angela Galdamez, Keon‐Hyoung Song, Masako Le, Kyle Kim, Jonathan H. Lin

{"title":"Ethnic Variation and Structure‐Function Analysis of Tauopathy‐Associated PERK Alleles","authors":"Goonho Park, Angela Galdamez, Keon‐Hyoung Song, Masako Le, Kyle Kim, Jonathan H. Lin","doi":"10.1002/ijch.202300173","DOIUrl":"https://doi.org/10.1002/ijch.202300173","url":null,"abstract":"EIF2AK3, also known as PERK, plays a pivotal role in cellular proteostasis, orchestrating the Unfolded Protein Response (UPR) and Integrated Stress Response (ISR) pathways. In addition to its central position in intracellular stress regulation, human GWAS identify EIF2AK3 as a risk factor in tauopathies, neurodegenerative diseases caused by aberrant tau protein accumulation. Guided by these genomic indicators, our investigation systematically analyzed human PERK variants, focusing on those with potential tauopathy linkages. We assembled a comprehensive data set of human PERK variants associated with Wolcott Rallison Syndrome (WRS), tauopathies, and bioinformatically predicted loss‐of‐function, referencing the gnomAD, Ensembl, and NCBI databases. We found extensive racial/ethnic variation in the prevalence of common PERK polymorphisms linked to tauopathies. Using SWISS‐MODEL, we identified structural perturbations in the ER stress‐sensing luminal domain dimers/oligomers of tauopathy‐associated PERK variants, Haplotypes A and B, in combination with another tauopathy‐linked R240H mutation. Recombinant expression of disease‐associated variants in vitro revealed altered PERK signal transduction kinetics in response to ER stress compared to the predominant non‐disease variant. In summary, our data further substantiates that human PERK variants identified in tauopathy genetic studies negatively impact PERK structure, function, and downstream signaling with significant variations in prevalence among different racial and ethnic groups.","PeriodicalId":14686,"journal":{"name":"Israel Journal of Chemistry","volume":null,"pages":null},"PeriodicalIF":3.2,"publicationDate":"2024-05-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141152436","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Porous Crystalline Organic Cages Made by Design 设计制造的多孔结晶有机笼

IF 2.3 4区化学

Israel Journal of Chemistry Pub Date : 2024-05-15 DOI: 10.1002/ijch.202400025

Dr. Svetlana Ivanova, Prof. Dr. Florian Beuerle

引用次数: 0