ISRN Toxicology最新文献_第3页

Cadmium Transport in a Model of Neonatal Intestinal Cells Correlates to MRP1 and Not DMT1 or FPN1. 新生儿肠细胞模型中的镉转运与MRP1而非DMT1或FPN1相关

ISRN Toxicology Pub Date : 2013-01-27 Print Date: 2013-01-01 DOI: 10.1155/2013/892364

Helena Ohrvik, Eva Tydén, Per Artursson, Agneta Oskarsson, Jonas Tallkvist

{"title":"Cadmium Transport in a Model of Neonatal Intestinal Cells Correlates to MRP1 and Not DMT1 or FPN1.","authors":"Helena Ohrvik, Eva Tydén, Per Artursson, Agneta Oskarsson, Jonas Tallkvist","doi":"10.1155/2013/892364","DOIUrl":"https://doi.org/10.1155/2013/892364","url":null,"abstract":"Newborns have a higher gastrointestinal uptake of cadmium than adults. In adults, the iron transporters DMT1 and FPN1 are involved in the intestinal absorption of cadmium, while in neonates, the mechanisms for cadmium absorption are unknown. We have investigated possible cadmium transporters in the neonatal intestine by applying a model of immature human intestinal epithelial Caco-2 cells. To mimic the continuous cadmium exposure via diet in neonates, cells were allowed to differentiate for 7 days in medium containing 1 μ M CdCl2. A dramatic upregulation of the MT1 gene expression followed cadmium pretreatment, indicating a high sensitivity of the immature cells to cadmium. Cadmium pretreatment increased the basolateral efflux of (109)Cd, without causing any effects on the passive diffusion of mannitol or the transepithelial electrical resistance. The augmented transport of cadmium was correlated to an upregulation of MRP1 gene expression and increased activity of the efflux protein MRP1. No effects were observed on gene expression of the efflux proteins MRP2 and P-gp or the iron transporters DMT1, DMT1-IRE and FPN1. In conclusion, our data indicate that continuous cadmium exposure increases the absorption of the metal in immature intestinal cells and that MRP1 is involved in the intestinal cadmium absorption in newborns.","PeriodicalId":14674,"journal":{"name":"ISRN Toxicology","volume":"2013 ","pages":"892364"},"PeriodicalIF":0.0,"publicationDate":"2013-01-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1155/2013/892364","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"31563728","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 14

Antinociceptive Activity and Redox Profile of the Monoterpenes (+)-Camphene, p-Cymene, and Geranyl Acetate in Experimental Models. 单萜烯(+)-莰烯、对伞花烯和香叶乙酸酯在实验模型中的抗伤活性和氧化还原谱。

ISRN Toxicology Pub Date : 2013-01-14 Print Date: 2013-01-01 DOI: 10.1155/2013/459530

Lucindo Quintans-Júnior, José C F Moreira, Matheus A B Pasquali, Soheyla M S Rabie, André S Pires, Rafael Schröder, Thallita K Rabelo, João P A Santos, Pollyana S S Lima, Sócrates C H Cavalcanti, Adriano A S Araújo, Jullyana S S Quintans, Daniel P Gelain

{"title":"Antinociceptive Activity and Redox Profile of the Monoterpenes (+)-Camphene, p-Cymene, and Geranyl Acetate in Experimental Models.","authors":"Lucindo Quintans-Júnior, José C F Moreira, Matheus A B Pasquali, Soheyla M S Rabie, André S Pires, Rafael Schröder, Thallita K Rabelo, João P A Santos, Pollyana S S Lima, Sócrates C H Cavalcanti, Adriano A S Araújo, Jullyana S S Quintans, Daniel P Gelain","doi":"10.1155/2013/459530","DOIUrl":"https://doi.org/10.1155/2013/459530","url":null,"abstract":"Objective. To evaluate antinocicpetive and redox properties of the monoterpenes (+)-camphene, p-cymene, and geranyl acetate in in vivo and in vitro experimental models. Methods. Evaluation of the in vitro antioxidant activity of (+)-camphene, p-cymene, and geranyl acetate using different free radical-generating systems and evaluation of antinociceptive actions by acetic acid-induced writhing and formalin-induced nociception tests in mice. Results. p-Cymene has the strongest antinociceptive effect, but (+)-camphene and geranyl acetate also present significant activity at high doses (200 mg/kg). (+)-Camphene had the strongest antioxidant effect in vitro at TBARS and TRAP/TAR assays and also had the highest scavenging activities against different free radicals, such as hydroxyl and superoxide radicals. Sodium nitroprussiate-derived NO production was enhanced by (+)-camphene. Geranyl acetate and p-cymene also presented some antioxidant effects, but with a varying profile according the free radical-generating system studied. Conclusion. (+)-Camphene, p-cymene, and geranyl acetate may present pharmacological properties related to inflammation and pain-related processes, being potentially useful for development of new therapeutic strategies, with limited possibilities for p-cymene and geranyl acetate.","PeriodicalId":14674,"journal":{"name":"ISRN Toxicology","volume":"2013 ","pages":"459530"},"PeriodicalIF":0.0,"publicationDate":"2013-01-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1155/2013/459530","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"31562718","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 112

Assessment of Polycyclic Aromatic Hydrocarbon Contamination of Breeding Pools Utilized by the Puerto Rican Crested Toad, Peltophryne lemur. 波多黎各冠蟾蜍、狐猴繁殖池多环芳烃污染评价。

ISRN Toxicology Pub Date : 2012-12-16 Print Date: 2012-01-01 DOI: 10.5402/2012/309853

Jenessa Gjeltema, Michael Stoskopf, Damian Shea, Ryan De Voe

引用次数: 0

In vitro-in vivo correlation evaluation of generic alfuzosin modified release tablets. 仿制药阿呋唑嗪改良缓释片的体内外相关性评价。

ISRN Toxicology Pub Date : 2012-11-20 Print Date: 2012-01-01 DOI: 10.5402/2012/813836

Utpal Kumar Sanki, Badal Kumar Mandal

{"title":"In vitro-in vivo correlation evaluation of generic alfuzosin modified release tablets.","authors":"Utpal Kumar Sanki, Badal Kumar Mandal","doi":"10.5402/2012/813836","DOIUrl":"https://doi.org/10.5402/2012/813836","url":null,"abstract":"Alfuzosin, a selective alpha-1a antagonistis is the most recently approved AARAS, with limited cardiac toxicity and exclusively used for lower urinary tract syndromes (LUTS). In order to reduce pill burden and better patient compliance modified release (MR) formulations have been developed. Alfuzosin MR tablet was developed by the use of hot-melt granulation techniques using mono- and diglycerides as rate controlling membranes to minimize health care cost and uses of costly excipients. The other purpose of the study was to evaluate in vitro-in vivo performance of the scale up batch in healthy human subjects for commercialization. The blend uniformity (mean ± RSD%), assay, cumulative percent dissolution at 24 h, hardness, and friability of the biobatch were 100.2 ± 0.05%, 100.43 ± 0.023%, 93.98%, 4.5 kg, 5 min, and 0.08%, respectively. The in vivo pharmacokinetic parameters under fasting conditions between test and reference formulations (Uroxatral 10 mg extended release tablets) were comparable. The 90% CI, geometric mean ratio (%) and power of C max, AUCT, and AUCI of the fasting study for the test and reference formulation were 99.03% to 122.78%, 109%, 0.998; 92.94% to 116.71%, 104%, 1; 98.17% to 124.01%, 110% 1, respectively. The scale up biobatch showed negligible difference in in vitro properties with respect to the pilot batch. The formulation developed with these agents was safe to use as there were no serious adverse events developed during the conduction of the clinical trial on the healthy subjects. Furthermore, the developed formulation was bioequivalent with respect to rate and extends of absorption to the reference formulation.","PeriodicalId":14674,"journal":{"name":"ISRN Toxicology","volume":"2012 ","pages":"813836"},"PeriodicalIF":0.0,"publicationDate":"2012-11-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.5402/2012/813836","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"31503909","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Nitric oxide synthase gene transfer overcomes the inhibition of wound healing by sulfur mustard in a human keratinocyte in vitro model. 一氧化氮合酶基因转移克服了硫芥菜对人角质细胞体外模型伤口愈合的抑制作用。

ISRN Toxicology Pub Date : 2012-11-14 Print Date: 2012-01-01 DOI: 10.5402/2012/190429

Hiroshi Ishida, Radharaman Ray, Jack Amnuaysirikul, Keiko Ishida, Prabhati Ray

{"title":"Nitric oxide synthase gene transfer overcomes the inhibition of wound healing by sulfur mustard in a human keratinocyte in vitro model.","authors":"Hiroshi Ishida, Radharaman Ray, Jack Amnuaysirikul, Keiko Ishida, Prabhati Ray","doi":"10.5402/2012/190429","DOIUrl":"https://doi.org/10.5402/2012/190429","url":null,"abstract":"Sulfur mustard (SM) is a chemical warfare agent that causes extensive skin injury. Previously we reported that SM exposure resulted in suppression of inducible nitric oxide synthase (iNOS) expression to inhibit the healing of scratch wounds in a cultured normal human epidermal keratinocyte (NHEK) model. Based on this finding, the present study was to use adenovirus-mediated gene transfer of iNOS to restore the nitric oxide (NO) supply depleted by exposure to SM and to evaluate the effect of NO on wound healing inhibited by SM in NHEKs. The effect of the iNOS gene transfer on iNOS protein expression and NO generation were monitored by Western blot and flow cytometry, respectively. Wound healing with or without the iNOS gene transfer after SM exposure was assessed by light and confocal microscopy. The iNOS gene transfer via adenovirus resulted in overexpression of the iNOS and an increase in NO production regardless of SM exposure in the NHEK model. The gene transfer was also effective in overcoming the inhibition of wound healing due to SM exposure leading to the promotion of wound closure. The findings in this study suggest that the iNOS gene transfer is a promising therapeutic strategy for SM-induced skin injury.","PeriodicalId":14674,"journal":{"name":"ISRN Toxicology","volume":"2012 ","pages":"190429"},"PeriodicalIF":0.0,"publicationDate":"2012-11-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.5402/2012/190429","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"31503903","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 7

Toxicity Assessment of Expired Pesticides to Green Algae Pseudokirchneriella subcapitata. 过期农药对绿藻类的毒性评价。

ISRN Toxicology Pub Date : 2012-11-14 Print Date: 2012-01-01 DOI: 10.5402/2012/247072

G Satyavani, G Chandrasehar, K Krishna Varma, A Goparaju, S Ayyappan, P Neelakanta Reddy, P Balakrishna Murthy

{"title":"Toxicity Assessment of Expired Pesticides to Green Algae Pseudokirchneriella subcapitata.","authors":"G Satyavani, G Chandrasehar, K Krishna Varma, A Goparaju, S Ayyappan, P Neelakanta Reddy, P Balakrishna Murthy","doi":"10.5402/2012/247072","DOIUrl":"https://doi.org/10.5402/2012/247072","url":null,"abstract":"In order to investigate the effect of expired pesticides on the yield and growth rate of green algae Pseudokirchneriella subcapitata, a study was conducted as per the Organisation for Economic Cooperation and Development (OECD) guideline number 201. Fifteen expired pesticide formulations, most commonly used in Indian agriculture, were tested in comparison with their unexpired counterparts. The expired pesticide formulations studied belonged to various class and functional groups: organophosphate, pyrethroid-based insecticides; azole-based fungicides; acetamide, propionate, acetic acid-based herbicides; fungicides mixtures containing two actives-azole and dithiocarbamate. The toxicity endpoints of yield (EyC50: 0-72 h) and growth rate (ErC50: 0-72 h) of Pseudokirchneriella subcapitata for each pesticide formulation (both expired and unexpired pesticides) were determined statistically using TOXSTAT 3.5 version software. The results pointed out that some expired pesticide formulations exhibited higher toxicity to tested algal species, as compared to the corresponding unexpired pesticides. These data thus stress the need for greater care to dispose expired pesticides to water bodies, to avoid the effects on aquatic ecospecies tested.","PeriodicalId":14674,"journal":{"name":"ISRN Toxicology","volume":"2012 ","pages":"247072"},"PeriodicalIF":0.0,"publicationDate":"2012-11-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.5402/2012/247072","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"31503905","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 14

Bmoo FIBMP-I: A New Fibrinogenolytic Metalloproteinase from Bothrops moojeni Snake Venom. Bmoo fimp - 1:一种新的毛蛇蛇毒纤维蛋白原溶解金属蛋白酶。

ISRN Toxicology Pub Date : 2012-11-04 Print Date: 2012-01-01 DOI: 10.5402/2012/673941

F S Torres, B Rates, M T R Gomes, C E Salas, A M C Pimenta, F Oliveira, M M Santoro, M E de Lima

{"title":"Bmoo FIBMP-I: A New Fibrinogenolytic Metalloproteinase from Bothrops moojeni Snake Venom.","authors":"F S Torres, B Rates, M T R Gomes, C E Salas, A M C Pimenta, F Oliveira, M M Santoro, M E de Lima","doi":"10.5402/2012/673941","DOIUrl":"https://doi.org/10.5402/2012/673941","url":null,"abstract":"A new fibrinogenolytic metalloproteinase (Bmoo FIBMP-I) was purified from Bothrops moojeni snake venom. This enzyme was isolated through a combination of three chromatographic steps (ion-exchange, molecular exclusion, and affinity chromatography). Analyses by reverse phase chromatography, followed by mass spectrometry, showed the presence of enzyme isoforms with average molecular mass of 22.8 kDa. The SDS-PAGE analyses showed a single chain of 27.6 kDa, in the presence and absence of reducing agent. The protein has a blocked N-terminal. One of the peptides obtained by enzymatic digestion of a reduced and S-alkylated isoform was completely sequenced by mass spectrometry (MS/MS). Bmoo FIBMP-I showed similarity with hemorrhagic factor and several metalloproteinases (MP). This enzyme degraded Aα-chain faster than the Bβ-chain and did not affect the γ-chain of bovine fibrinogen. The absence of proteolytic activity after treatment with EDTA, together with the observed molecular mass, led us to suggest that Bmoo FIBMP-I is a member of the P-I class of the snake venom MP family. Bmoo FIBMP-I showed pH-dependent proteolytic activity on azocasein, but was devoid of coagulant, defibrinating, or hemorrhagic activities. The kinetic parameters of proteolytic activity in azocasein were determined (V max = 0.4596 Uh(-1)nmol(-1) ± 0.1031 and K m = 14.59 mg/mL ± 4.610).","PeriodicalId":14674,"journal":{"name":"ISRN Toxicology","volume":"2012 ","pages":"673941"},"PeriodicalIF":0.0,"publicationDate":"2012-11-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.5402/2012/673941","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"31503908","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 9

In vitro effects of herbicides and insecticides on human breast cells. 除草剂和杀虫剂对人乳腺细胞的体外影响。

ISRN Toxicology Pub Date : 2012-10-14 Print Date: 2012-01-01 DOI: 10.5402/2012/232461

Jessica D Rich, Seth M Gabriel, Jennifer R Schultz-Norton

{"title":"In vitro effects of herbicides and insecticides on human breast cells.","authors":"Jessica D Rich, Seth M Gabriel, Jennifer R Schultz-Norton","doi":"10.5402/2012/232461","DOIUrl":"https://doi.org/10.5402/2012/232461","url":null,"abstract":"Numerous studies have indicated that the pesticides and herbicides used in agricultural processes in the United States and Europe may have detrimental effects upon human health. Many of these compounds have been indicated as potential endocrine and reproductive disruptors, although the studies have examined supraphysiological levels well above the US EPA safe levels for drinking water and have often examined these effects in \"model\" cell lines such as Chinese hamster ovary cells. We have now examined the cytotoxicity of more environmentally relevant concentrations of four herbicides, acetochlor, atrazine, cyanazine, and simazine, and two insecticides, chlorpyrifos and resmethrin, in three human breast cell lines. Interestingly, cytotoxicity was not observed in the estrogen-dependent MCF-7 mammary epithelial carcinoma cells; rather increases in cell viability were seen for some of the compounds at select concentrations. These results vary greatly from what was observed in the estrogen independent MDA-MB-231 breast cancer cells and the non-cancerous MCF-10A breast cells. This gives insight into how different tumors may respond to pesticide exposure and allows us to make more accurate conclusions about the potential cytotoxicity or, at times, stimulatory actions of these pesticides.","PeriodicalId":14674,"journal":{"name":"ISRN Toxicology","volume":"2012 ","pages":"232461"},"PeriodicalIF":0.0,"publicationDate":"2012-10-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.5402/2012/232461","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"31503904","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 27

Heavy metal quantification in renal tissue of patients in the state of yucatan and its association with urolithiasis. 尤卡坦州患者肾组织重金属定量及其与尿石症的关系。

ISRN Toxicology Pub Date : 2012-09-27 Print Date: 2012-01-01 DOI: 10.5402/2012/548256

Luis A May-Ix, J Gabriel Rosado-Rubio, Martha Medina-Escobedo, Arturo F Castellanos-Ruelas, Luis A Chel-Guerrero, David A Betancur-Ancona

{"title":"Heavy metal quantification in renal tissue of patients in the state of yucatan and its association with urolithiasis.","authors":"Luis A May-Ix, J Gabriel Rosado-Rubio, Martha Medina-Escobedo, Arturo F Castellanos-Ruelas, Luis A Chel-Guerrero, David A Betancur-Ancona","doi":"10.5402/2012/548256","DOIUrl":"https://doi.org/10.5402/2012/548256","url":null,"abstract":"A possible cause associated with urinary lithiasis (UL) is the bioaccumulation of heavy metals in the kidney. The aim of this study was to evaluate the content of Cu, Pb, and Cd in kidney tissues removed from patients with nephrological problems and associate it with UL. Samples of 50 kidney sections from patients were analyzed. Results were statistically analyzed using a fixed effects model including the overall mean, the effect of the health status of patients (with or without UL), gender (male and female), the interaction between both factors and the random error (NID (0, σ (2))). Cu level was 8.8 ± 4.4 mg/kg (mean ± DS) and 25.5% of samples had levels above normal. Lead content in 97.9% of the samples (3.6 ± 1.5 mg/kg) was above normal. All results of Cd (13.2 ± 16.6 mg/kg) were below the maximum permissible limits. There was no difference in the amount of heavy metals on patients with or without UL (P > 0.05) nor depending on the gender (P > 0.05). It was concluded that there is no apparent relationship between a very elevated level of Cu or Pb in the kidney on the development of UL.","PeriodicalId":14674,"journal":{"name":"ISRN Toxicology","volume":"2012 ","pages":"548256"},"PeriodicalIF":0.0,"publicationDate":"2012-09-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.5402/2012/548256","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"31503907","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 4

Affinity and matrix effects in measuring fish plasma vitellogenin using immunosorbent assays: considerations for aquatic toxicologists. 用免疫吸附法测定鱼血浆卵黄蛋白原的亲和力和基质效应:水生毒理学家的考虑。

ISRN Toxicology Pub Date : 2012-09-18 Print Date: 2012-01-01 DOI: 10.5402/2012/942804

Stephen E Bartell, Heiko L Schoenfuss

{"title":"Affinity and matrix effects in measuring fish plasma vitellogenin using immunosorbent assays: considerations for aquatic toxicologists.","authors":"Stephen E Bartell, Heiko L Schoenfuss","doi":"10.5402/2012/942804","DOIUrl":"https://doi.org/10.5402/2012/942804","url":null,"abstract":"Enzyme-linked immunosorbent assays (ELISAs) are important tools in aquatic toxicology and have become crucial in assessing exposure concentrations in the aquatic environment and acute physiological responses in exposed organisms. These assays utilize the inherent properties of antibodies to recognize and selectively bind a target molecule, while largely ignoring other molecules to provide semiquantitative values. A variety of methodologies to measure plasma vitellogenin using ELISAs have generated widely divergent data. Limitations of the ELISA method are known in the wider immunology field, though aquatic toxicologists may be less familiar with these limitations. We evaluated several mechanisms contributing to the divergent vitellogenin data in the literature. Antibody affinities and the matrix in which standard curves are constructed are possible error generators. These errors can be amplified by large sample dilutions necessary to fall within the standard curve. It is important for the aquatic toxicology research community to realize the limitations and understand the pitfalls of absolute plasma vitellogenin data in their studies.","PeriodicalId":14674,"journal":{"name":"ISRN Toxicology","volume":"2012 ","pages":"942804"},"PeriodicalIF":0.0,"publicationDate":"2012-09-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.5402/2012/942804","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"31595093","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 12