ISJ-Invertebrate Survival Journal最新文献

New data on C1qDC from the colonial ascidian Botryllus schlosseri 殖民地海鞘菌(Botryllus schlosseri) C1qDC的新资料

IF 0.7 4区农林科学

ISJ-Invertebrate Survival Journal Pub Date : 2021-10-29 DOI: 10.25431/1824-307X/ISJ.V18I1.130-137

A. Peronato, G. Minervini, N. Franchi, L. Ballarin

引用次数: 0

Transcriptomic analysis of strain-specific and gender-specific response of silkworm to BmNPV infection 家蚕对BmNPV感染的株特异性和性别特异性转录组学分析

IF 0.7 4区农林科学

ISJ-Invertebrate Survival Journal Pub Date : 2021-08-27 DOI: 10.25431/1824-307X/ISJ.V18I1.98-107

S. He, J. Xu, Y. Fan, F. Zhu, Kan-Shu Chen

{"title":"Transcriptomic analysis of strain-specific and gender-specific response of silkworm to BmNPV infection","authors":"S. He, J. Xu, Y. Fan, F. Zhu, Kan-Shu Chen","doi":"10.25431/1824-307X/ISJ.V18I1.98-107","DOIUrl":"https://doi.org/10.25431/1824-307X/ISJ.V18I1.98-107","url":null,"abstract":"Bombyx mori nucleopolyhedrovirus (BmNPV) is one of the main pathogens causing serious economic losses in sericulture. However, the molecular mechanism of silkworm resistance to BmNPV is still largely unclear, and the differences in the anti-BmNPV response between silkworms of different genders have been rarely studied. In this study, BmNPV resistant strain NB and BmNPV sensitive strain 306 of different genders were used as experimental materials to inoculate BmNPV, and their transcriptomes were sequenced to analyze their response to BmNPV. Eighteen genes specifically differentially expressed in NB after BmNPV inoculation were finally obtained through transcriptomic analysis, fourteen of which were up-regulated and four were down-regulated, suggesting that they might be related to BmNPV resistance. Among them, the expression abundance of eight genes were higher in males than in females, and one gene was in the contrary. These genes suggested that there were certain differences in the anti-BmNPV response between silkworms of different genders. This study provided a new understanding of the molecular mechanism of silkworm resistance to BmNPV and the differences in the anti-BmNPV response between silkworms of different genders, and laid a foundation for future prevention and control of BmNPV.","PeriodicalId":14623,"journal":{"name":"ISJ-Invertebrate Survival Journal","volume":null,"pages":null},"PeriodicalIF":0.7,"publicationDate":"2021-08-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"86268965","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

A C-type lectin (AjSJL-1) containing DPN/WVD motifs in Apostichopus japonicus recognizes multiple microbes Apostichopus japonicus中含有DPN/WVD基序的c型凝集素(AjSJL-1)可识别多种微生物

IF 0.7 4区农林科学

ISJ-Invertebrate Survival Journal Pub Date : 2021-06-29 DOI: 10.25431/1824-307X/ISJ.V18I1.86-97

Qi Zhao, Hui Wang, Weilin Wang, Jiaxiang Li, Yu Liu, Zhuang Xue, Zhaoqun Liu, Lingling Wang, L. Song

{"title":"A C-type lectin (AjSJL-1) containing DPN/WVD motifs in Apostichopus japonicus recognizes multiple microbes","authors":"Qi Zhao, Hui Wang, Weilin Wang, Jiaxiang Li, Yu Liu, Zhuang Xue, Zhaoqun Liu, Lingling Wang, L. Song","doi":"10.25431/1824-307X/ISJ.V18I1.86-97","DOIUrl":"https://doi.org/10.25431/1824-307X/ISJ.V18I1.86-97","url":null,"abstract":"C-type lectins (CTLs) are a superfamily of Ca2+ dependent carbohydrate-recognition proteins with diversified functions ranging from embryonic development to immune defense. In the present study, a CTL containing only one CRD domain with new motifs Asp-Pro-Asn (DPN) and Trp-Val-Asp (WVD) in its Ca2+ binding site 2 (designated as AjSJL-1) was identified from sea cucumber Apostichopus japonicus. The deduced amino acid sequence of AjSJL-1 was homologous to CTLs from other animals with the identities ranging from 19 % to 28.4 %. The mRNA transcripts of AjSJL-1 were detected in all the examined tissues with varied abundance. The expression level of AjSJL-1 mRNA in coelomocyte was up-regulated significantly at 12 h after Vibrio splendidus challenge. The recombinant protein of AjSJL-1 (rAjSJL-1) displayed significant binding activity to lipopolysaccharide, peptidoglycan, mannose and D-galactose in a Ca2+-dependent manner. Moreover, rAjSJL-1 exhibited strong binding capability to V. splendidus but week to Staphylococcus aureus, Bifidobacterium breve, Pichia pastoris and Yarrowia lipolytica in the presence of Ca2+. These results collectively suggested that AjSJL-1 with new DPN/WVD motifs served as a pattern recognition receptor in sea cucumber with the capability to bind broad-spectrum microbes and initiate the immune response against invaders.","PeriodicalId":14623,"journal":{"name":"ISJ-Invertebrate Survival Journal","volume":null,"pages":null},"PeriodicalIF":0.7,"publicationDate":"2021-06-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"75722656","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Immunological and oxidative responses of the lesser mulberry pyralid, Glyphodes pyloalis by an aqueous extract of Artemisia annua L. 黄花蒿水提取物对小桑树pyloalis的免疫和氧化反应。

IF 0.7 4区农林科学

ISJ-Invertebrate Survival Journal Pub Date : 2021-06-24 DOI: 10.25431/1824-307X/ISJ.V18I1.75-85

Z. Afraze, Jalal Jalali Sendi

引用次数: 4

A Field-Friendly Loop-Mediated Isothermal Amplification (FF-LAMP) method for rapid detection of Nosema bombycis in silkworm, Bombyx mori 现场友好环介导等温扩增(FF-LAMP)方法快速检测家蚕微孢子虫

IF 0.7 4区农林科学

ISJ-Invertebrate Survival Journal Pub Date : 2021-06-21 DOI: 10.25431/1824-307X/ISJ.V18I1.66-74

V. Sivaprasad, L. Satish, G. Mallikarjuna, N. Chandrakanth, A. Josepha, S. M. Moorthy

{"title":"A Field-Friendly Loop-Mediated Isothermal Amplification (FF-LAMP) method for rapid detection of Nosema bombycis in silkworm, Bombyx mori","authors":"V. Sivaprasad, L. Satish, G. Mallikarjuna, N. Chandrakanth, A. Josepha, S. M. Moorthy","doi":"10.25431/1824-307X/ISJ.V18I1.66-74","DOIUrl":"https://doi.org/10.25431/1824-307X/ISJ.V18I1.66-74","url":null,"abstract":"Pebrine is a destructive disease that exhibits horizontal and vertical transmission and therefore it is the only mandatory quarantine item in sericulture. Here, a field-friendly loop-mediated isothermal amplification (FF-LAMP) method has been developed and validated for the rapid detection of Nosema bombycis, a causative agent of pebrine disease in silkworm, Bombyx mori. FF-LAMP primers were selected and designed for small ribosomal subunit gene and the assay was performed to detect the N. bombycis infection in silkworm. The FF-LAMP reaction was effective at 6 mM MgSO4, 1.4 mM dNTPs at 63 °C. The detection range of LAMP assay was found to be 101 dilutions of N. bombycis spores. Specificity of the primers was tested using DNA isolated from pebrine infected silkworm, pebrine free silkworm and pure N. bombycis by conventional PCR and FF-LAMP assay. Results revealed that the primers were specific to N. bombycis DNA. The FF-LAMP assay was validated in different basic silkworm seed farms with simultaneous microscopic examination of N. bombycis infection. This newly developed method is highly effective, specific, sensitive and rapid in detecting N. bombycis infection, eliminating the DNA purification steps and usage of sophisticated equipment. This method can be used in testing large number of samples making it field friendly method in sericulture industry.","PeriodicalId":14623,"journal":{"name":"ISJ-Invertebrate Survival Journal","volume":null,"pages":null},"PeriodicalIF":0.7,"publicationDate":"2021-06-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"88217506","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

A diet rich in diatom improves the antibacterial capacity of Pacific oyster Crassostrea gigas by enhancing norepinephrine-regulated immunomodulation 富含硅藻的饲料通过增强去甲肾上腺素调节的免疫调节来提高太平洋牡蛎长牡蛎的抗菌能力

IF 0.7 4区农林科学

ISJ-Invertebrate Survival Journal Pub Date : 2021-04-14 DOI: 10.25431/1824-307X/ISJ.V18I1.56-65

Q. Sun, Yan Zheng, X. Chen, Ning Kong, Yi Wang, Yukun Zhang, Y. Zong, Zhaoqun Liu, Lingling Wang, L. Song

{"title":"A diet rich in diatom improves the antibacterial capacity of Pacific oyster Crassostrea gigas by enhancing norepinephrine-regulated immunomodulation","authors":"Q. Sun, Yan Zheng, X. Chen, Ning Kong, Yi Wang, Yukun Zhang, Y. Zong, Zhaoqun Liu, Lingling Wang, L. Song","doi":"10.25431/1824-307X/ISJ.V18I1.56-65","DOIUrl":"https://doi.org/10.25431/1824-307X/ISJ.V18I1.56-65","url":null,"abstract":"Microalgae such as dinoflagellate and diatom are the major food source of bivalve species, and sufficient food intake contributes to the immunity and the growth of bivalves. In the present study, a monoamine oxidase gene (named as CgMAO), which is the rate-limiting enzyme of norepinephrine (NE) biosynthesis, was cloned from C. gigas. After the oysters were fed with a diet rich in diatom for 21 and 40 d, the NE contents in oyster serum, as well as the mRNA expression of CgMAO in oyster haemocytes, increased significantly compared with control group. Besides, the mRNA expression of cytokines CgTNF-1 and CgIL17-5 in haemocytes and the activities of immune-related enzymes (SOD and LYZ) in oyster serum also increased significantly after diatom feeding. These results collectively suggested that sufficient microalgae intake might significantly enhance the antibacterial capacity in oyster by prompting the biosynthesis of NE and triggering the subsequent antibacterial processes modulated by NE.","PeriodicalId":14623,"journal":{"name":"ISJ-Invertebrate Survival Journal","volume":null,"pages":null},"PeriodicalIF":0.7,"publicationDate":"2021-04-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"79433759","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The temporal dynamics of bacteria in the coelomic fluid of sea cucumber Apostichopus japonicus after evisceration 海参去内脏后体腔液中细菌的时间动态

IF 0.7 4区农林科学

ISJ-Invertebrate Survival Journal Pub Date : 2021-02-24 DOI: 10.25431/1824-307X/ISJ.V18I1.46-55

C. Zhang, Zichao Yu, Zhuang Xue, Huan Li, J. Zhu, Liyan Wang, L. Song

{"title":"The temporal dynamics of bacteria in the coelomic fluid of sea cucumber Apostichopus japonicus after evisceration","authors":"C. Zhang, Zichao Yu, Zhuang Xue, Huan Li, J. Zhu, Liyan Wang, L. Song","doi":"10.25431/1824-307X/ISJ.V18I1.46-55","DOIUrl":"https://doi.org/10.25431/1824-307X/ISJ.V18I1.46-55","url":null,"abstract":"Sea cucumbers have been emerged as important models to study organ regeneration and development owing to the capacity to regenerate its organs quickly after evisceration. Evisceration is a special defense mechanism for sea cucumber to eject all of internal organs when they encounter predators or adverse environmental conditions. However, little was known about the dynamics of bacterial community in coelomic fluid after evisceration. In the present study, evisceration was induced by intracelomic injection of 0.35 M KCl, and the significantly alternation of bacterial community in coelomic fluid of sea cucumber Apostichopus japonicus was observed with lower diversity and total bacterial load at 7 dpe (days post evisceration) and 14 dpe. The bacterial community was tended to restore at 28 dpe. In particular, relative abundances of Bacteroidetes and Rubritaleaceae, which involved in degradation of polysaccharides and lipid, increased significantly at 7 dpe (p < 0.05), and returned to the original level at 28 dpe. In addition, the predicted functions of bacterial community indicated that the bacteria associated with metabolism pathways of amino acid, lipid and carbohydrate also increased significantly at 7 dpe. These results suggested that the bacterial community in coelomic fluid of A. japonicus was highly dynamic and could rebuild a stable community structure after evisceration. It was suggested that the enriched metabolic related beneficial bacteria at early stage played a role after evisceration in terms of decomposing polysaccharides and lipid to provide energy.","PeriodicalId":14623,"journal":{"name":"ISJ-Invertebrate Survival Journal","volume":null,"pages":null},"PeriodicalIF":0.7,"publicationDate":"2021-02-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"80549561","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 2

The influence of surface waters on the bioavailability and toxicity of copper oxide nanoparticles to freshwater mussels 地表水对氧化铜纳米颗粒对淡水贻贝的生物利用度和毒性的影响

IF 0.7 4区农林科学

ISJ-Invertebrate Survival Journal Pub Date : 2021-02-17 DOI: 10.25431/1824-307X/ISJ.V18I1.33-45

J. Auclair, P. Turcotte, C. Gagnon, C. Gagne

{"title":"The influence of surface waters on the bioavailability and toxicity of copper oxide nanoparticles to freshwater mussels","authors":"J. Auclair, P. Turcotte, C. Gagnon, C. Gagne","doi":"10.25431/1824-307X/ISJ.V18I1.33-45","DOIUrl":"https://doi.org/10.25431/1824-307X/ISJ.V18I1.33-45","url":null,"abstract":"The increased commercial use of copper oxide nanoparticles (nCuO) led to the release of nanoparticles in wastewaters potentially harming the aquatic biota. The purpose of this study was to determine the toxic action of nCuO and dissolved Cu (II) to Dreissena bugensis freshwater mussels placed in 4 types of surface waters: aquarium, green (high conductivity), brown (high organic carbon) and 10 % municipal effluent (high conductivity and anthropogenic source of organic carbon). Mussels were exposed to 50 µg/L of nCuO or Cu (II) for 96 h at 15 °C in the above waters. The results revealed that the total Cu loadings were higher in mussels placed in organic-rich waters (brown and effluent) and exposed to either forms of Cu. Tissue Cu contents were correlated with air-time survival, lipid peroxidation, protein-ubiquitin levels and DNA strand breaks. Both surface water types and Cu forms influenced Zn (II) mobilization, glutathione S-transferase activity and protein turnover (ubiquitin binding). Based on the surface water properties, Cu (II) was more influenced by the levels and origin of the organic carbon content while nCuO was more influenced by the total suspended solids. In conclusion the toxicity of nCuO could be influenced by surface waters properties expecially when similar physiological targets are impacts by these treatments.","PeriodicalId":14623,"journal":{"name":"ISJ-Invertebrate Survival Journal","volume":null,"pages":null},"PeriodicalIF":0.7,"publicationDate":"2021-02-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"89348523","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Evolutionary insights on a novel mussel-specific foot protein-3 gene family 一个新的贻贝特异性足蛋白-3基因家族的进化见解

IF 0.7 4区农林科学

ISJ-Invertebrate Survival Journal Pub Date : 2021-01-20 DOI: 10.25431/1824-307X/ISJ.V18I1.19-32

E. Bortoletto, P. Venier, A. Figueras, B. Novoa, U. Rosani

{"title":"Evolutionary insights on a novel mussel-specific foot protein-3 gene family","authors":"E. Bortoletto, P. Venier, A. Figueras, B. Novoa, U. Rosani","doi":"10.25431/1824-307X/ISJ.V18I1.19-32","DOIUrl":"https://doi.org/10.25431/1824-307X/ISJ.V18I1.19-32","url":null,"abstract":"Silky byssus threads enable a number of marine and freshwater bivalve mollusks to attach themselves to hard substrates. Byssus production is an energy-costly process, which accompany the switch from planktonic to sessile life. Pointing the attention to a small foot protein (fp-3α) first identified in Perna viridis and abundantly secreted during the bissogenesis, we report the presence of a fp-3α gene family in species of the Mytilus complex, byssogenic bivalve mollusks mostly inhabiting marine waters. In the genome of Mytilus galloprovincialis we identified twelve fp-3α genes showing differences in exon-intron organization and suggesting that, as in the case of arthropod and mollusk defensins, exon shuffling could have played an important role in the evolution of this gene family. Also, the different tissue expression patterns of these mussel genes support their functional diversification. All predicted fp-3α proteins curiously possess a Csαβ three-dimensional motif based on 10 highly conserved cysteines and exhibit structural similarity to invertebrate defensins. The role of these small cysteine-rich proteins in supporting the byssus-mediated mussel adhesion or their action as host defence peptides remains to be established with further study.","PeriodicalId":14623,"journal":{"name":"ISJ-Invertebrate Survival Journal","volume":null,"pages":null},"PeriodicalIF":0.7,"publicationDate":"2021-01-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"76616200","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

A cold bath for a formalin-free laboratory: alternative fixative methods in early developmental stages of the sea urchin Paracentrotus lividus (Lamarck, 1816) 无福尔马林实验室的冷水浴:海胆(Paracentrotus lividus)早期发育阶段的几种固定方法(Lamarck, 1816)

IF 0.7 4区农林科学

ISJ-Invertebrate Survival Journal Pub Date : 2021-01-15 DOI: 10.25431/1824-307X/ISJ.V18I1.11-18

A. Cannavacciuolo, Antonia Chiarore, M. Munari

{"title":"A cold bath for a formalin-free laboratory: alternative fixative methods in early developmental stages of the sea urchin Paracentrotus lividus (Lamarck, 1816)","authors":"A. Cannavacciuolo, Antonia Chiarore, M. Munari","doi":"10.25431/1824-307X/ISJ.V18I1.11-18","DOIUrl":"https://doi.org/10.25431/1824-307X/ISJ.V18I1.11-18","url":null,"abstract":"Fixatives are widespread in biological and medical research because they allow preserving specimens for a long time. Historically, formaldehyde has been the most used fixative so far, but new solutions are needed because of its carcinogenicity. In this study, we tested alternative fixative methods to find a harmless, economic, and simple-to-use methodology to fix samples for larval morphological analysis in Paracentrotus lividus. In two separate experiments, P. lividus embryos were fixed after 48 h post-fertilization by adding Formalin Free Tissue AccustainTM, NaOH-buffered Formalin Free Tissue AccustainTM, glacial ethanol and denatured ethanol at different concentrations (from 10 % to 70 %) and by submerging the vials containing the larvae in seawater at 0 °C and maintained at 4 °C for 144 h. Our results suggested that all the alternative fixatives tested do not guarantee a good quality of larvae for morphological purposes, while larvae that faced the thermal shock and were kept at 4 °C did not show any evidence of damage throughout time. The results of this study candidate this method as a good and safe substitute of formalin in studies that require morphological and taxonomic recognition and shed light on its use in other kinds of studies as well.","PeriodicalId":14623,"journal":{"name":"ISJ-Invertebrate Survival Journal","volume":null,"pages":null},"PeriodicalIF":0.7,"publicationDate":"2021-01-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"90933569","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 4