Chemistry - An Asian Journal最新文献_第2页

Synthesis and Electronic Features of M-N Bonding of N-Heterocyclic Imine Coordinated Alkali-Metal Amides [MN(SiMe₃)₂, M = Li, Na, K]. n -杂环亚胺配位碱金属酰胺M- n键的合成及电子特性[MN(SiMe3)2, M = Li, Na, K]。

IF 3.2 3区化学

Chemistry - An Asian Journal Pub Date : 2026-11-01 DOI: 10.1002/asia.71038

Ayantika Das, Sofia A Khan, Bindusagar Das, Ritu Yadav, Balvinder Singh, Sajesh P Thomas, Subrata Kundu

{"title":"Synthesis and Electronic Features of M-N Bonding of N-Heterocyclic Imine Coordinated Alkali-Metal Amides [MN(SiMe3)2, M = Li, Na, K].","authors":"Ayantika Das, Sofia A Khan, Bindusagar Das, Ritu Yadav, Balvinder Singh, Sajesh P Thomas, Subrata Kundu","doi":"10.1002/asia.71038","DOIUrl":"https://doi.org/10.1002/asia.71038","url":null,"abstract":"N-Heterocyclic imine (NHI) ligands are attractive due to their strong electron-donating properties, and their applications in coordination chemistry continue to expand. In this study, we investigated the reactivity of MN(SiMe3)2 (M = Li, Na, K) toward (tBu 2NHC)NH, and (Me 2CAAC)NH [NHC = N-heterocyclic carbene, CAAC = cyclic (alkyl)(amino)carbene]. The basicity of MN(SiMe3)2 (M = Li, Na, K) was insufficient to deprotonate the N─H bond of these carbene-derived imines; instead, coordination complexes were formed. The lithium derivative was isolated with the general formula [{(carbene)NH}2LiN(SiMe3)2], whereas the sodium and potassium bis(trimethylsilyl)amide analogues crystallized as [{(carbene)NH}2{MN(SiMe3)2}2] (M = Na, K). The compounds were thoroughly characterized by multinuclear NMR spectroscopy and single-crystal x-ray diffraction. Furthermore, the x-ray quantum crystallographic (QCr) technique of Hirshfeld Atom Refinement (HAR) revealed hydrogen atom positions and electronic features of the M─N bonds.","PeriodicalId":145,"journal":{"name":"Chemistry - An Asian Journal","volume":"21 21","pages":""},"PeriodicalIF":3.2,"publicationDate":"2026-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149369743","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Surface-Modified Dysprosium-Trimesic Acid Metal-Organic Frameworks for the Fluorescent Detection of Coumaphos. 表面修饰的三聚酸镝金属-有机骨架用于复方福的荧光检测。

IF 3.2 3区化学

Chemistry - An Asian Journal Pub Date : 2026-11-01 DOI: 10.1002/asia.71040

Karanveer Singh, M Manolata Devi, Suvankar Chakraverty, Bhanu Prakash

{"title":"Surface-Modified Dysprosium-Trimesic Acid Metal-Organic Frameworks for the Fluorescent Detection of Coumaphos.","authors":"Karanveer Singh, M Manolata Devi, Suvankar Chakraverty, Bhanu Prakash","doi":"10.1002/asia.71040","DOIUrl":"https://doi.org/10.1002/asia.71040","url":null,"abstract":"We report a novel Dy-BTC metal-organic framework (DTM) platform and its surface-engineered derivatives for highly sensitive, label-free photoluminescent detection of the organophosphate (Op) pesticide coumaphos (CP) in ethanol. Systematic control of synthesis parameters produced a highly crystalline, intensely luminescent DTM scaffold (M/L = 2:1; solvothermal, 120°C; pH 7; EtOH; 24 h), which exhibited selective PL quenching by CP across 0.25-250 ppb. Targeted surface functionalization of DTM with L-ascorbic acid (L-AA) and 11-mercaptoundecanoic acid (MUDA) introduced -COOH and -SH recognition motifs that markedly enhanced analyte affinity and quenching efficiency via noncovalent interactions and electron/energy transfer. The Stern-Volmer constants increased from (1 × 1010) L·mol- 1 (DTM) to (4 × 1010) L·mol- 1 (DT-AA) and (3 × 1010) L·mol- 1 (DT-MUDA), while limits of detection improved from 30.40 ppb (DTM) to 4.89 ppb (DT-AA) and 3.65 ppb (DT-MUDA). The sensing of CP at single-digit ppb levels with good linearity (R2 > 0.96) and low material cost highlights the potential of combining MOF building-unit design with post-synthetic surface engineering. The DT-AA and DT-MUDA platforms therefore provide promising proof-of-concept candidates for subsequent matrix validation and development of portable CP screening approaches for environmental and food-related applications.","PeriodicalId":145,"journal":{"name":"Chemistry - An Asian Journal","volume":"21 21","pages":""},"PeriodicalIF":3.2,"publicationDate":"2026-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149369742","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Adsorption Technology for Lithium Extraction: Recent Progress and Future Perspectives 锂萃取吸附技术研究进展及展望

IF 3.2 3区化学

Chemistry - An Asian Journal Pub Date : 2026-10-31 DOI: 10.1002/asia.71033

Jie Kang, Bowen Shi, Zhenbin Wang, Zhufeng Lu, Zhen Guo, Zhengping Dong

{"title":"Adsorption Technology for Lithium Extraction: Recent Progress and Future Perspectives","authors":"Jie Kang, Bowen Shi, Zhenbin Wang, Zhufeng Lu, Zhen Guo, Zhengping Dong","doi":"10.1002/asia.71033","DOIUrl":"https://doi.org/10.1002/asia.71033","url":null,"abstract":"<div>\u0000 \u0000 Lithium, a critical enabler of the new energy paradigm, has made its efficient recovery a strategic priority worldwide. Among direct lithium extraction (DLE) technologies, adsorption stands out for its superior Mg/Li selectivity and low energy intensity when processing salt-lake brines. This review critically surveys recent breakthroughs in inorganic ion sieves, covering anion-dependent behavior and magnetic functionalization of Li/Al-LDHs, synergistic doping–coating strategies for Mn-based sieves, and atomic-scale engineering of Ti-based systems. It further examines emerging adsorbents, including MOFs, COFs, and crown-ether hybrids, with respect to their lithium-recognition mechanisms and application potential. On this basis, the review distills key scientific bottlenecks and engineering obstacles, and outlines transformative pathways forward, notably machine-learning-assisted material design, electrochemical–adsorption integration, and recovery from ultra-dilute resources. Unlike previous reviews that focus on individual material classes, this review establishes a unified cross-category framework that links synthesis dimensions, evidence levels, performance trade-offs, and application-specific decision rules for adsorptive lithium extraction. In doing so, it aims to provide a cohesive framework for both fundamental inquiry and industrial deployment of adsorptive lithium extraction.\u0000 </div>","PeriodicalId":145,"journal":{"name":"Chemistry - An Asian Journal","volume":"21 21","pages":""},"PeriodicalIF":3.2,"publicationDate":"2026-10-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149312828","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Selenocarbonyl Synthetic Strategies, Molecular Diversification, and Applications of C═Se-Containing Compounds 含C =硒化合物的硒羰基合成策略、分子多样化和应用

IF 3.2 3区化学

Chemistry - An Asian Journal Pub Date : 2026-10-31 DOI: 10.1002/asia.71030

Malak Ghufran Ullah, Muhammad Akram, Jie Liu, Mamoona Sadaf, Xinrong Cheng, Wenbin Yi

{"title":"Selenocarbonyl Synthetic Strategies, Molecular Diversification, and Applications of C═Se-Containing Compounds","authors":"Malak Ghufran Ullah, Muhammad Akram, Jie Liu, Mamoona Sadaf, Xinrong Cheng, Wenbin Yi","doi":"10.1002/asia.71030","DOIUrl":"https://doi.org/10.1002/asia.71030","url":null,"abstract":"<div>\u0000 \u0000 Selenocarbonyl compounds are an important class of organoselenium compounds with unique reactivity, distinct electronic properties, and versatile biological activities. This review critically summarizes the main synthetic methods for C═Se derivatives, including selenoamides, selenoformamides, selenoureas, selenocarbamates, isoselenocyanates, and selenosemicarbazones. It covers both classical selenium-transfer reagents (Woollins’ reagent, phosphorus pentaselenide, LiAlHSeH, metal selenides, selenium dioxide, potassium selenocyanate, and elemental selenium) and recent one-pot, multicomponent, and nucleophilic strategies. Rather than treating the literature as a chronological catalogue, the discussion is organized around shared selenium sources, reactive intermediates, and downstream functions, and explicitly compares reaction conditions, substrate scope, isolated yields, operational simplicity, product stability, safety, and scalability. The review further describes applications in selenium-containing heterocycles, metal complexes, bioactive molecules, supramolecular systems, and functional materials, highlighting reported anticancer, antimicrobial, antioxidant, anti-inflammatory, and enzyme-inhibitory activities. A dedicated discussion of 77Se NMR spectroscopy clarifies how selenium chemical shifts connect molecular structure, electronic properties, and coordination behavior. Despite notable progress, many existing methods still rely on toxic or moisture-sensitive reagents, strong bases, elevated temperatures, or tedious purification, and biological and materials-related applications remain largely underexplored. Future research should focus on safer selenium sources, simpler scalable reactions, improved product stability, and better integration of molecular design with practical application.\u0000 </div>","PeriodicalId":145,"journal":{"name":"Chemistry - An Asian Journal","volume":"21 21","pages":""},"PeriodicalIF":3.2,"publicationDate":"2026-10-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149312850","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Sustainable Syngas Production From CO2-Assisted Gasification of Solid Wastes: A Simulation-Based Approach to CO2 Utilization 固体废物二氧化碳辅助气化可持续合成气生产：基于模拟的二氧化碳利用方法

IF 3.2 3区化学

Chemistry - An Asian Journal Pub Date : 2026-10-31 DOI: 10.1002/asia.71025

Yaneeporn Patcharavorachot, Pimchanok Anuthawong, Puttarak Wisuwong, Dang Saebea, Amornchai Arpornwichanop

{"title":"Sustainable Syngas Production From CO2-Assisted Gasification of Solid Wastes: A Simulation-Based Approach to CO2 Utilization","authors":"Yaneeporn Patcharavorachot, Pimchanok Anuthawong, Puttarak Wisuwong, Dang Saebea, Amornchai Arpornwichanop","doi":"10.1002/asia.71025","DOIUrl":"https://doi.org/10.1002/asia.71025","url":null,"abstract":"<div>\u0000 \u0000 CO2-assisted gasification offers a potential route for waste valorization and CO2 utilization, yet the suitability of chemically distinct wastes under comparable operating conditions remains insufficiently established. This study compares wood residue (WR), rice straw (RS), poultry litter (PL), and high-density polyethylene (HDPE) using an Aspen Plus equilibrium model. The effects of temperature (500°C–1000°C) and gasifying agent-to-waste (G/W) mass ratio (0.5–3.0) on syngas composition, H2/CO ratio, gas yield, lower heating value, cold gas efficiency, and net heat duty were evaluated. The feedstocks exhibited distinct thermodynamic responses. Biomass-derived feedstocks generally showed improved gas-phase performance with increasing temperature, whereas HDPE exhibited substantially higher gas yields and a distinct response to CO2 availability. Contour-based trade-off analysis and constraint-based screening were further applied to identify feedstock-specific feasible operating regions. Within the predefined operating window of 700°C–900°C and G/W = 0.5–2.5, using H2/CO = 1–2 and CGE ≤ 100% as screening criteria, HDPE exhibited a broad feasible region, WR showed a highly restricted region, and no feasible regions were identified for PL and RS. These findings provide a systematic framework for preliminary feedstock and operating-domain screening for CO2-assisted waste-to-syngas conversion.\u0000 </div>","PeriodicalId":145,"journal":{"name":"Chemistry - An Asian Journal","volume":"21 21","pages":""},"PeriodicalIF":3.2,"publicationDate":"2026-10-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149312785","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

LED Light-Induced Modulation of the Anticancer Activity of a [(2-(6-Bromopyridin-2-yl)-1H-benzo[d]imidazol-6-yl)(phenyl)methanone]-Based Iridium(III) Complex [（2-（6-溴吡啶-2-基）- 1h -苯并[d]咪唑-6-基）（苯基）甲烷]基铱（III）配合物抗癌活性的LED光诱导调制

IF 3.2 3区化学

Chemistry - An Asian Journal Pub Date : 2026-10-30 DOI: 10.1002/asia.71034

Utpal Das, Pavan Gutti, Rishav Das, Shanooja Shanavas, Debasish Mishra, Sudheer Shenoy P, Bipasha Bose, Priyankar Paira

{"title":"LED Light-Induced Modulation of the Anticancer Activity of a [(2-(6-Bromopyridin-2-yl)-1H-benzo[d]imidazol-6-yl)(phenyl)methanone]-Based Iridium(III) Complex","authors":"Utpal Das, Pavan Gutti, Rishav Das, Shanooja Shanavas, Debasish Mishra, Sudheer Shenoy P, Bipasha Bose, Priyankar Paira","doi":"10.1002/asia.71034","DOIUrl":"10.1002/asia.71034","url":null,"abstract":"<div>\u0000 \u0000 Metal complexes are emerging as powerful agents in light-activated cancer therapy, a cutting-edge therapeutic strategy for combating cancer and other serious diseases. Among them, iridium (Ir)-based photosensitizers have recently gained significant attention, driving innovation in photo-activated cancer therapy and opening new avenues for targeted treatment. Building on this momentum, we have designed a novel benzimidazole-based iridium complex, [LIr], which demonstrates remarkable potential as a phototherapeutic agent. Upon activation with a direct light source from a 40 W white LED (400−700 nm), with a fixed distance of 15 cm between the light source and the samples maintained throughout all the experiments, [LIr] exhibits potent catalytic activity, efficiently oxidizing key cellular antioxidants: converting glutathione (GSH) to glutathione disulfide (GSSG), NADH to NAD+, and NADPH to NADP+. This redox disruption compromises cancer cell defenses and enhances therapeutic efficacy. In addition, light activation of complex [LIr] generates a burst of reactive oxygen species (ROS), including the highly cytotoxic singlet oxygen, with a notable singlet oxygen quantum yield. This multifaceted reactivity not only disrupts cellular redox balance but also induces oxidative stress in targeted cancer cells.\u0000 </div>","PeriodicalId":145,"journal":{"name":"Chemistry - An Asian Journal","volume":"21 21","pages":""},"PeriodicalIF":3.2,"publicationDate":"2026-10-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149293454","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Surfactant-Assisted Cascade Synthesis of Tetrahydroquinoline Cyanocarboxamides Through Intramolecular Hydride-Transfer Cyclization in Aqueous CTAB Media 表面活性剂在CTAB水溶液中通过分子内氢化物转移环化辅助级联合成四氢喹啉氰羧酰胺。

IF 3.2 3区化学

Chemistry - An Asian Journal Pub Date : 2026-10-30 DOI: 10.1002/asia.71037

Rodney A. Fernandes, Rahul D. Mandal

引用次数: 0

Copper-Catalyzed C─N Cross-Coupling in Water Enabled by Rational Ligand Design and Micellar Cooperativity 合理配体设计和胶束协同性使铜催化的水中C─N交叉偶联成为可能。

IF 3.2 3区化学

Chemistry - An Asian Journal Pub Date : 2026-10-29 DOI: 10.1002/asia.71031

Purusottam Pradhan, Rajan Yadav, Shalini Kumari, Susanta Hazra

{"title":"Copper-Catalyzed C─N Cross-Coupling in Water Enabled by Rational Ligand Design and Micellar Cooperativity","authors":"Purusottam Pradhan, Rajan Yadav, Shalini Kumari, Susanta Hazra","doi":"10.1002/asia.71031","DOIUrl":"10.1002/asia.71031","url":null,"abstract":"<div>\u0000 \u0000 Nitrogen-containing compounds are ubiquitous in pharmaceuticals, agrochemicals, and functional materials, and the development of sustainable methodologies for carbon–nitrogen (C─N) bond formation is of fundamental importance. Herein, we report a rationally designed bifunctional ligand and micellar cooperativity that effectively enables Cu-catalyzed amination of aryl bromides under mild aqueous conditions. A series of structurally tailored ligands was designed by systematically tuning their electronic and steric properties to stabilize the active copper catalyst and promote selective C─N bond formation. When combined with the bio-derived non-ionic surfactant CNSL-1000-M, the micellar environment provides a confined hydrophobic reaction space, enhancing catalyst performance and substrate activation. This cooperative ligand–micelle system enables the C─N coupling with both electron-rich and electron-deficient aryl bromides at 60°C, affording the corresponding aryl amines in good to excellent yields. The active Cu catalyst was thoroughly characterized by UV–visible spectroscopy, x-ray photoelectron spectroscopy (XPS), and Electron Paramagnetic Resonance (EPR). It is worth noting that our current catalyst system shows better catalytic activity and selectivity than conventional organic solvents such as DMF, 1,4-dioxane, DMSO, and toluene. This study demonstrates that electronic, steric, and micellar cooperativity play decisive roles in aqueous copper-catalyzed reactions and establishes a practical, sustainable platform for C─N cross-coupling reactions.\u0000 </div>","PeriodicalId":145,"journal":{"name":"Chemistry - An Asian Journal","volume":"21 21","pages":""},"PeriodicalIF":3.2,"publicationDate":"2026-10-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149293474","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

BICAAC-Catalyzed Hydroboration of N-Heteroarenes and Imines: A Metal-Free Route to Reduced N-Heterocycles and Amines bicaac催化n -杂环烯和亚胺的硼化反应：一条无金属还原n -杂环和胺的途径。

IF 3.2 3区化学

Chemistry - An Asian Journal Pub Date : 2026-10-29 DOI: 10.1002/asia.71024

Alisha Sharma, Surbhi Bansal, Sanjay Singh

{"title":"BICAAC-Catalyzed Hydroboration of N-Heteroarenes and Imines: A Metal-Free Route to Reduced N-Heterocycles and Amines","authors":"Alisha Sharma, Surbhi Bansal, Sanjay Singh","doi":"10.1002/asia.71024","DOIUrl":"10.1002/asia.71024","url":null,"abstract":"<div>\u0000 \u0000 Catalytic hydroboration of N-heteroarenes and imines represents an attractive and sustainable strategy for accessing a broad range of synthetically valuable nitrogen-containing compounds. Herein, we disclose the metal-free catalytic activity of bicyclic (alkyl)(amino)carbene (BICAAC) (I) toward the hydroboration of N-heteroarenes and imines using pinacolborane (HBpin). The catalyst exhibits excellent regioselectivity, promoting 1,4-hydroboration of pyridines and 1,2-hydroboration of quinolines, while efficiently reducing a variety of imines under mild reaction conditions. Mechanistic investigations, based on a series of control experiments, reveal that the reaction is initiated by the addition of the B─H bond of HBpin across the carbene center to generate the catalytically active BICAAC-borane species (II). This intermediate activates the substrate through a Lewis acid–base interaction between the boron center and the nitrogen atom of the substrate, followed by hydride transfer from a second equivalent of HBpin to afford the hydroborated products. This work significantly expands the scope of BICAAC organocatalysis and demonstrates its potential as an efficient metal-free catalyst for the reduction of challenging nitrogen-containing unsaturated substrates.\u0000 </div>","PeriodicalId":145,"journal":{"name":"Chemistry - An Asian Journal","volume":"21 21","pages":""},"PeriodicalIF":3.2,"publicationDate":"2026-10-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149293480","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Subzero-Temperature NMR Observation of Protein Dynamics and Ligand Binding Enabled by Encapsulation Within Coordination Cages 低温核磁共振观察蛋白质动力学和配体结合在配位笼内的封装。

IF 3.2 3区化学

Chemistry - An Asian Journal Pub Date : 2026-10-29 DOI: 10.1002/asia.71029

Risa Ebihara, Takahiro Nakama, Miri Tadokoro, Maho Yagi-Utsumi, Koichi Kato, Makoto Fujita

{"title":"Subzero-Temperature NMR Observation of Protein Dynamics and Ligand Binding Enabled by Encapsulation Within Coordination Cages","authors":"Risa Ebihara, Takahiro Nakama, Miri Tadokoro, Maho Yagi-Utsumi, Koichi Kato, Makoto Fujita","doi":"10.1002/asia.71029","DOIUrl":"10.1002/asia.71029","url":null,"abstract":"Lowering temperatures provides an effective strategy for probing rapid protein dynamics. However, conventional nuclear magnetic resonance (NMR) measurements are usually limited to temperatures above 0°C due to water freezing and increasing rotational correlation times. In this study, we report subzero-temperature NMR analysis enabled by encapsulating a protein within spherical coordination cages. Spatial confinement within the metallo-cages stabilizes proteins by preventing aggregation, enabling multi-dimensional NMR measurements of a 20 kDa protein at temperatures as low as −30°C in low-viscosity acetone–water mixtures. Below 0°C, the NMR spectra of the protein revealed markedly slowed dynamics, resolving two distinct conformations as split signals. Furthermore, the subzero-temperature analysis enabled detection of exchangeable hydroxy protons that are unobservable at ambient temperatures. Saturation transfer difference (STD) NMR revealed binding of carbohydrate hydroxy groups to lysozyme, owing to the large apertures of the cages, which permit ligand diffusion while the cages stabilize proteins under subzero conditions. This cage confinement thus facilitates the study of rapid protein dynamics and ligand binding beyond the limits of conventional solution NMR.","PeriodicalId":145,"journal":{"name":"Chemistry - An Asian Journal","volume":"21 21","pages":""},"PeriodicalIF":3.2,"publicationDate":"2026-10-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/asia.71029","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149293488","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0