Juan Pérez Quezadas, Yesica Guadalupe Cabrera Sillas, Rogelio Monreal, Miguel Rangel Medina, José Iván Morales Arredondo, Ricardo Sánchez-Murillo
{"title":"Hydrochemistry and stable isotopes revealed focused and diffuse recharge processes in the Sonora River basin, Mexico.","authors":"Juan Pérez Quezadas, Yesica Guadalupe Cabrera Sillas, Rogelio Monreal, Miguel Rangel Medina, José Iván Morales Arredondo, Ricardo Sánchez-Murillo","doi":"10.1080/10256016.2023.2171032","DOIUrl":"https://doi.org/10.1080/10256016.2023.2171032","url":null,"abstract":"<p><p>A hydro-geochemical characterization was conducted in the northern part of the Sonora River basin, covering an area of 9400 km<sup>2</sup>. Equipotential lines indicated that groundwater circulation coincided with the surface water flow direction. Based on the groundwater temperature measured (on average ∼21 °C), only one spring exhibited thermalism (51 °C). Electrical conductivity (160-1750 <i>μ</i>S/cm), chloride and nitrate concentrations (>10 and >45 mg/L) imply highly ionized water and anthropogenic pollution. In the river network, <i>δ</i><sup>18</sup>O values revealed a clear modern meteoric origin. Focused recharge occurred mainly from the riverbeds during the rainy season. During the dry season, diffuse recharge was characterized by complex return flows from irrigation, urban, agricultural, mining, and livestock. Drilled wells (>50 m) exhibited a strong meteoric origin from higher elevations during the rainy season with minimal hydrochemical anomalies. Our results contribute to the knowledge of mountain-front and mountain-block recharge processes in a semi-arid and human-altered landscape in northern Mexico, historically characterized by limited hydrogeological data.</p>","PeriodicalId":14597,"journal":{"name":"Isotopes in Environmental and Health Studies","volume":"59 1","pages":"48-65"},"PeriodicalIF":1.3,"publicationDate":"2023-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9523321","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Advances in the microbiological diagnosis of herpetic retinitis.","authors":"Julie Gueudry, Bahram Bodaghi","doi":"10.3389/fopht.2022.990240","DOIUrl":"10.3389/fopht.2022.990240","url":null,"abstract":"<p><p>Viral retinitis associated with herpesvirus is one of the most severe forms of uveitis and is a potentially sight-threatening ophthalmologic disease. The prognosis is poor and a rapid and aggressive management is necessary to improve the visual and sometimes vital prognosis of these patients. The treatments used are not without side effects, while many differential diagnoses exist, such as toxoplasmic retinochoroiditis, syphilitic retinitis, endogenous endophthalmitis and intraocular lymphoma. Causatives viruses are herpes simplex virus, varicella-zoster virus, and cytomegalovirus, which require rapid detection in ocular fluid, mainly aqueous humor. However, only a small amount of intraocular fluid is available for analysis. Advances in microbiological diagnostic techniques therefore were key factors in improving the management of these diseases. Historically, the diagnosis was based on immunological tests but more recently advances in molecular biology, in particular polymerase chain reaction, have played a crucial role to obtain a reliable and rapid diagnosis of viral retinitis associated with herpesvirus, as discussed in this review.</p>","PeriodicalId":14597,"journal":{"name":"Isotopes in Environmental and Health Studies","volume":"7 1","pages":"990240"},"PeriodicalIF":0.0,"publicationDate":"2022-09-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11182275/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"81678676","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Adeel A Butt, Victor B Talisa, Peng Yan, Obaid S Shaikh, Saad B Omer, Florian B Mayr
{"title":"Vaccine Effectiveness of 3 Versus 2 Doses of Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2) mRNA Vaccines in a High-Risk National Population.","authors":"Adeel A Butt, Victor B Talisa, Peng Yan, Obaid S Shaikh, Saad B Omer, Florian B Mayr","doi":"10.1093/cid/ciac178","DOIUrl":"10.1093/cid/ciac178","url":null,"abstract":"<p><strong>Background: </strong>Knowledge of the vaccine effectiveness (VE) of a third or booster vaccine dose in preventing SARS-CoV-2 infection or its consequences is critical in developing recommendations for their use. We determined relative VE of 3 vs 2 doses of an mRNA vaccine in preventing symptomatic SARS-CoV-2 infection, hospitalization, and severe/critical disease.</p><p><strong>Methods: </strong>Among veterans who had received 2 doses of an mRNA vaccine by 30 April 2021, we identified those who received a third dose of the same vaccine between 22 September and 24 November 2021 and 1:1 matched controls who had not received their third dose by then. Using Cox proportional hazards model, we calculated adjusted hazards ratios for symptomatic infection, hospitalization, and intensive care unit (ICU) admission or death after SARS-CoV-2-positive test.</p><p><strong>Results: </strong>Among 2 321 366 veterans who received 2 doses of Pfizer BNT-162b2 or Moderna mRNA-1273 vaccine by 30 April 2021, we matched 395 686 persons who received a third dose of the same vaccine between 22 September and 24 November 2021 to controls who did not receive a third dose. Adjusted HRs (95% CI) were .15 (.11-.21) for symptomatic infection and .18 (.13-.26) for hospitalizations for 3 vs 2 doses, corresponding to relative VE of 85% and 82%. Five ICU admissions or deaths were observed (4 among recipients of 2 doses). There was no difference in VE between BNT162b2 versus mRNA-1273 recipients.</p><p><strong>Conclusions: </strong>A third dose of a SARS-CoV-2 mRNA vaccine is associated with high VE against symptomatic infection, hospitalization, and critical disease in the pre-Omicron era.</p>","PeriodicalId":14597,"journal":{"name":"Isotopes in Environmental and Health Studies","volume":"12 1","pages":"e579-e584"},"PeriodicalIF":0.0,"publicationDate":"2022-08-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8903438/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"81697630","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
C Canales-Cerro, E Hidalgo-Hermoso, J Cabello, I Sacristán, A Cevidanes, S Di Cataldo, C Napolitano, D Moreira-Arce, S Klarian, J Millán
{"title":"Carbon and nitrogen isotopic similarity between the endangered Darwin's fox (<i>Lycalopex fulvipes</i>) and sympatric free-ranging dogs in Chiloé Island, Chile.","authors":"C Canales-Cerro, E Hidalgo-Hermoso, J Cabello, I Sacristán, A Cevidanes, S Di Cataldo, C Napolitano, D Moreira-Arce, S Klarian, J Millán","doi":"10.1080/10256016.2022.2106225","DOIUrl":"https://doi.org/10.1080/10256016.2022.2106225","url":null,"abstract":"<p><p>Darwin's fox is an opportunistic omnivorous predator native to Chile classified as endangered by the IUCN Red List. Habitat use by Darwin's foxes can be negatively affected by the presence of free-ranging dogs that range freely across native and non-native habitats and can be a source of fox mortality. The objective of this study was to analyze the isotopic similarity of Darwin's fox and sympatric free-ranging dogs in Chiloé Island to determine the impact of anthropogenic environmental alterations on wild predators. We use hair samples to characterise and compare their <i>δ</i><sup>13</sup>C and <i>δ</i><sup>15</sup>N values and to evaluate isotopic similarity and isotope niches overlap. A generalised linear model was used to associate the isotope value with landscape variables (forest cover and vegetation type) and distance to the nearest house. We found no significant differences in <i>δ</i><sup>13</sup>C or <i>δ</i><sup>15</sup>N values between foxes and dogs, and a marginally significant isotope niche overlap (59.4 %). None of the selected variables at landscape and site scale were related to isotope values. Although our study is not a probe of direct contact between foxes and free-ranging dogs, the high isotopic similarity highlights the risk of pathogen spillover from free-ranging dogs to Darwin's foxes.</p>","PeriodicalId":14597,"journal":{"name":"Isotopes in Environmental and Health Studies","volume":"58 4-6","pages":"316-326"},"PeriodicalIF":1.3,"publicationDate":"2022-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10341089","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Stable isotope patterns of German rivers with aspects on scales, continuity and network status.","authors":"Paul Koeniger, Christine Stumpp, Axel Schmidt","doi":"10.1080/10256016.2022.2127702","DOIUrl":"https://doi.org/10.1080/10256016.2022.2127702","url":null,"abstract":"<p><p>In Germany, river monitoring for tritium started in the early 1970s. Today this monitoring network consists of 50 stations and includes stable isotopes. The stable isotope time series to the end of 2021 are at least four years and for some stations up to 30 years long. Daily river water samples were collected during an extraordinary dry season from October 2018 until end of January 2019 from six selected stations of the Rhine and five stations of the Elbe basin. The most dominating stable isotope effects in river water are the seasonal and altitude effects, but also a continental effect is visible. The isotopes indicate snow and ice melt contributions in the Rhine and Danube during the summer months and a consecutive dilution of these signals by mixing with tributary rivers. Close to the coasts in northern Germany, stable isotope patterns reflect influence of seawater and tides. Daily patterns during the dry season 2018/2019 surprisingly do not exhibit extreme changes but rather trends of enhanced groundwater contribution. Long-term continual data across scales are important for comparing and identifying hydrological processes in German river basins of different size and mean catchment altitudes, and highlight the benefits of a co-organized national network.</p>","PeriodicalId":14597,"journal":{"name":"Isotopes in Environmental and Health Studies","volume":"58 4-6","pages":"363-379"},"PeriodicalIF":1.3,"publicationDate":"2022-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10403448","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Yuvraj Date, Shreyas Managave, Girish Jathar, Rahul Khot, Keith A Hobson
{"title":"Stable sulphur isotope (<i>δ</i><sup>34</sup>S) ratios in bird feathers from India indicate strong segregation between the Himalaya and Gangetic plain, and the rest of India.","authors":"Yuvraj Date, Shreyas Managave, Girish Jathar, Rahul Khot, Keith A Hobson","doi":"10.1080/10256016.2022.2113995","DOIUrl":"https://doi.org/10.1080/10256016.2022.2113995","url":null,"abstract":"<p><p>Although stable hydrogen (<i>δ</i><sup>2</sup>H) and carbon (<i>δ</i><sup>13</sup>C) isotopic compositions of bird feathers have been increasingly used to understand the migration of birds through assignment of individuals to established isoscapes, far less is known about the structure of feather isoscapes based on stable sulphur isotope (<i>δ</i><sup>34</sup>S) assays. While <i>δ</i><sup>2</sup>H isoscapes have been used to investigate the migratory origins of the Globe Skimmer (<i>Pantala flavescens</i>) dragonfly in India, no isotope studies have been applied to avian migration patterns in that region. Here, based on museum collections, we report the feather <i>δ</i><sup>34</sup>S values of 13 avian species that included marine birds with expected <sup>34</sup>S enriched feathers, resident birds throughout India and a single migratory species, Bar-headed Goose (<i>Anser indicus</i>). Feathers from the marine birds were the most enriched (20.6 ± 1.2 ‰, <i>n</i> = 10; range = 4.4 ‰) in <sup>34</sup>S but terrestrial resident species also showed unexpectedly high <i>δ</i><sup>34</sup>S values (11.7 ± 4.8 ‰, <i>n</i> = 74; range = 19.9 ‰) throughout most of India but the Himalayan region and Gangetic plain typically showed <i>δ</i><sup>34</sup>S values less than 6.4 ‰. Our results show the utility of feather <i>δ</i><sup>34</sup>S studies to decipher avian and other animal migration between these two regions.</p>","PeriodicalId":14597,"journal":{"name":"Isotopes in Environmental and Health Studies","volume":"58 4-6","pages":"327-339"},"PeriodicalIF":1.3,"publicationDate":"2022-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10341337","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Soil gas radon, indoor radon and its diurnal variation in the northern region of Cameroon.","authors":"Sadjo, Takoukam Soh Serge Didier, Hamadou Yerima Abba, Vaskanglang Valentin, Saïdou, Mohamadou Alidou","doi":"10.1080/10256016.2022.2102617","DOIUrl":"https://doi.org/10.1080/10256016.2022.2102617","url":null,"abstract":"<p><p>Soil gas radon and indoor radon measurements have been carried out in Mayo-Louti and Benoué Divisions in northern Cameroon. Concentrations of radon in soil have been measured using Markus 10 at the depth of about 1 m. Radon concentration in soil varies from 0.9 to 13.8 kBq m<sup>-3</sup> with a mean value of 4.6 kBq m<sup>-3</sup>. Average daily indoor radon concentrations measured with RadonEye+<sup>2</sup> detectors vary from 7 to 60 Bq m<sup>-3</sup> with an average of 17 Bq m<sup>-3</sup>. Indoor radon concentrations measured with passive RADTRAK detectors range between 15 and 104 Bq m<sup>-3</sup> with a geometric value of 38 Bq m<sup>-3</sup> and a geometric standard deviation of 1.5. This geometric value is lower than the value of 30 Bq m<sup>-3</sup> given by UNSCEAR. Indoor radon inhalation dose ranges between 0.28 and 1.97 mSv a<sup>-1</sup> with geometric value of 0.72 mSv a<sup>-1</sup> (at 0.03 standard deviation). Outdoor radon inhalation ranges between 0.02 and 0.26 mSv a<sup>-1</sup> with a mean value of 0.09 mSv a<sup>-1</sup>. The total annual effective dose due to indoor and outdoor radon exposure for this study area is 0.81 mSv a<sup>-1</sup>, less than 1.15 mSv a<sup>-1</sup> the world average value given by UNSCEAR. There is no significant radiological risk for the inhabitants.</p>","PeriodicalId":14597,"journal":{"name":"Isotopes in Environmental and Health Studies","volume":"58 4-6","pages":"402-419"},"PeriodicalIF":1.3,"publicationDate":"2022-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10690383","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Monika Maria Ciężka, Maciej Górka, Agnieszka Trzyna, Magdalena Modelska, Anna Łubek, David Widory
{"title":"The multi-isotope biogeochemistry (S, C, N and Pb) of <i>Hypogymnia physodes</i> lichens: air quality approach in the Świętokrzyski National Park, Poland.","authors":"Monika Maria Ciężka, Maciej Górka, Agnieszka Trzyna, Magdalena Modelska, Anna Łubek, David Widory","doi":"10.1080/10256016.2022.2110591","DOIUrl":"https://doi.org/10.1080/10256016.2022.2110591","url":null,"abstract":"<p><p>The isotope biogeochemistry of bioindicators has widely demonstrated its added value in environmental issues by allowing to precisely identify sources of contamination. Most of the studies are based on studying one or two isotope systematics. Here, we are presenting an innovative multi-proxy approach that combines chemistry with both stable (C, S, N) and radiogenic (Pb) isotope systematics. Using <i>Hypogymnia physodes</i> bioindicators, we evaluated air quality in the complex environment of the Świętokrzyski National Park (ŚNP, Poland) with the ultimate objective of isotopically identifying the sources responsible for the observed contamination. Combining the isotope systematics showed that home heating is a major source of contamination in winter, whereas the contribution of road traffic increases during the summer. Pb isotope ratios identified industrial activities as the major source of this metal in the atmosphere.</p>","PeriodicalId":14597,"journal":{"name":"Isotopes in Environmental and Health Studies","volume":"58 4-6","pages":"340-362"},"PeriodicalIF":1.3,"publicationDate":"2022-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10690410","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Yuliya Vystavna, Johannes Cullmann, Keith Hipel, Jodie Miller, David X Soto, Astrid Harjung, Andrew Watson, Alexandra Mattei, Seifu Kebede, Maksym Gusyev
{"title":"Better understand past, present and future climate variability by linking water isotopes and conventional hydrometeorology: summary and recommendations from the International Atomic Energy Agency and World Meteorological Organization.","authors":"Yuliya Vystavna, Johannes Cullmann, Keith Hipel, Jodie Miller, David X Soto, Astrid Harjung, Andrew Watson, Alexandra Mattei, Seifu Kebede, Maksym Gusyev","doi":"10.1080/10256016.2022.2108418","DOIUrl":"https://doi.org/10.1080/10256016.2022.2108418","url":null,"abstract":"Better understand past, present and future climate variability by linking water isotopes and conventional hydrometeorology: summary and recommendations from the International Atomic Energy Agency and World Meteorological Organization Yuliya Vystavna , Johannes Cullmann, Keith Hipel, Jodie Miller, David X. Soto, Astrid Harjung, Andrew Watson, Alexandra Mattei, Seifu Kebede f and Maksym Gusyev Isotope Hydrology Section, International Atomic Energy Agency (IAEA), Vienna International Centre, Vienna, Austria; World Meteorological Organization (WMO), Geneva, Switzerland; Department of Systems Design Engineering, University of Waterloo, Waterloo, Canada; School for Climate Studies, Stellenbosch University, Matieland, South Africa; UMR 6134 SPE, CNRS, University of Corsica, Corte, France; Center for Water Resources Research, University of KwaZulu Natal, Pietermaritzburg, South Africa; Institute of Environmental Radioactivity, Fukushima University, Fukushima, Japan","PeriodicalId":14597,"journal":{"name":"Isotopes in Environmental and Health Studies","volume":"58 4-6","pages":"311-315"},"PeriodicalIF":1.3,"publicationDate":"2022-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9111213","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Elizabeth Avery, Olena Samonina, Lidiia Kryshtop, Iryna Vyshenska, Alan E Fryar, Andrea M Erhardt
{"title":"Use of isotopes in examining precipitation patterns in north-central Ukraine.","authors":"Elizabeth Avery, Olena Samonina, Lidiia Kryshtop, Iryna Vyshenska, Alan E Fryar, Andrea M Erhardt","doi":"10.1080/10256016.2022.2131781","DOIUrl":"https://doi.org/10.1080/10256016.2022.2131781","url":null,"abstract":"<p><p>North-central Ukraine is vulnerable to temperature increases and precipitation pattern changes associated with climate change. With water management becoming increasingly important, information on current water sources and moisture recycling is critically needed. Isotope ratios of oxygen (<i>δ</i><sup>18</sup>O) and hydrogen (<i>δ</i><sup>2</sup>H) in precipitation are sensitive to these variables and allow comparisons across the region. The <i>δ</i><sup>2</sup>H and <i>δ</i><sup>18</sup>O values from collected precipitation in Kyiv and Cherkasy in 2020 and published <sup>3</sup>H data for Kyiv from the year 2000 show an influence of the North Atlantic Oscillation (NAO) and provide information about processes affecting precipitation along the storm trajectory. The <i>δ</i><sup>18</sup>O values also show a correlation with temperature, indicating that precipitation patterns may be affected by the rising temperatures in the region, as predicted by recent regional studies using Representative Concentration Pathway scenarios and the global climate model GFDL-ESM2M. When compared to backtracked storm trajectory and NAO data, clear relationships emerged between water isotope ratios, storm paths, and likely moisture recycling. Overall, <i>δ</i><sup>2</sup>H, <i>δ</i><sup>18</sup>O, <sup>3</sup>H, and backtracked storm trajectory data provide more regional and local information on water vapour processes, improving climate-change-driven precipitation forecasts in Ukraine.</p>","PeriodicalId":14597,"journal":{"name":"Isotopes in Environmental and Health Studies","volume":"58 4-6","pages":"380-401"},"PeriodicalIF":1.3,"publicationDate":"2022-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10342509","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}