Iranian journal of allergy, asthma, and immunology最新文献_第9页

Different Gene Expression Patterns of IL-1 Family Members in Parkinson's Disease: Results from Bayesian Regression Model. 帕金森病中 IL-1 家族成员的不同基因表达模式：贝叶斯回归模型的结果

IF 1.5 4区医学

Iranian journal of allergy, asthma, and immunology Pub Date : 2024-02-11 DOI: 10.18502/ijaai.v23i1.14955

Negin Jafariaghdam, Majid Khoshmirsafa, Alireza Zamani, Elahe Talebi-Ghane, Shadi Moradi, Faezeh Shahba, Mehrdokht Mazdeh, Mohammad Mahdi Eftekharian

{"title":"Different Gene Expression Patterns of IL-1 Family Members in Parkinson's Disease: Results from Bayesian Regression Model.","authors":"Negin Jafariaghdam, Majid Khoshmirsafa, Alireza Zamani, Elahe Talebi-Ghane, Shadi Moradi, Faezeh Shahba, Mehrdokht Mazdeh, Mohammad Mahdi Eftekharian","doi":"10.18502/ijaai.v23i1.14955","DOIUrl":"10.18502/ijaai.v23i1.14955","url":null,"abstract":"Parkinson's disease, the second most prevalent neurodegenerative disorder lacking a recognized etiology, is influenced by oxidative stress and alterations in inflammatory cytokine levels. This study aimed to investigate the expression levels of Interleukin(IL)1 receptor accessory protein (IL-1RAcP), IL1β, IL1α, IL33, and IL36 genes in blood cells and serum IL-1β levels in Parkinson's disease patients compared to healthy controls (HCs).I n this case-control study, 44 Parkinson's disease patients and 44 age- and sex-matched HCs were included. Gene expression levels were assessed using Quantitative Real-time PCR, and serum IL-1β levels were measured via enzyme-linked immunosorbent assay. Advanced statistical analyses using the Bayesian regression model in R software were employed. Parkinson's disease patients exhibited elevated expression levels of IL-1RAcP and IL1β genes but decreased levels of IL1α, IL33, and IL36 compared to HCs. Age-based differences were not significant. Regarding gender, IL33 transcript levels were significantly higher in males, and serum IL-1β levels were increased in patients. Subgroup analysis by gender indicated alterations in IL1β and IL-1RAcP expression in both genders, while IL1α, IL33, and IL36 showed reduced expression only in males. Remarkably, only female patients displayed significantly higher serum IL-1β levels than female HCs. These findings suggest that dysregulation of immune-related factors plays a crucial role in Parkinson's disease.","PeriodicalId":14560,"journal":{"name":"Iranian journal of allergy, asthma, and immunology","volume":"23 1","pages":"69-81"},"PeriodicalIF":1.5,"publicationDate":"2024-02-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140131412","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Unraveling the Impact of Blood Transfusion on Transcription Factors Regulating T Helper 1, 2, 17 and Regulatory T cells. 揭示输血对调节 T 辅助细胞 1、2、17 和调节性 T 细胞的转录因子的影响。

IF 1.5 4区医学

Iranian journal of allergy, asthma, and immunology Pub Date : 2024-02-11 DOI: 10.18502/ijaai.v23i1.14958

Samad Valizadeh, Azita Chegini, Faranak Behnaz, Ali Akbar Pourfatollah, Shahram Samiee, Ronak Karbalaeifar

{"title":"Unraveling the Impact of Blood Transfusion on Transcription Factors Regulating T Helper 1, 2, 17 and Regulatory T cells.","authors":"Samad Valizadeh, Azita Chegini, Faranak Behnaz, Ali Akbar Pourfatollah, Shahram Samiee, Ronak Karbalaeifar","doi":"10.18502/ijaai.v23i1.14958","DOIUrl":"10.18502/ijaai.v23i1.14958","url":null,"abstract":"T helper 1 (TH1) and TH2 lymphocytes are the most important components of the immune system affected by blood transfusion. This study aimed`` to evaluate the effect of blood transfusion on gene expression of transcription factors related to the development of TH1, TH2, TH17 and regulatory T cells (Tregs). In this cross-sectional study, 20 patients diagnosed with abdominal aortic aneurysms requiring surgical repair were studied from January 2018 to August 2020. We utilized real-time PCR to evaluate the expression of transcription factor genes associated with TH1, TH2, TH17, and Treg, namely T-box-expressed-in-T-cells (T-bet), GATA-binding protein 3 (GATA-3), retinoid-related orphan receptor (RORγt), and fork head box protein 3 (Foxp3), respectively. The sampling occurred before anesthesia, 24- and 72 hours post-transfusion, and at the time of discharge. The results showed that the T-bet gene expression, compared to the time before transfusion, was significantly decreased 24 hours after blood transfusion and upon discharge while GATA3 genes exhibited a significant reduction both 24 and 72 hours after the transfusion, as compared to the pre-transfusion levels and the time of patient discharge. The Foxp3 gene demonstrated an increase at all study stages, with a notable surge, particularly 72 hours after red blood cell (RBC) transfusion. Conversely, the expression of RORγt gene, consistently decreased throughout all stages of the study. RBC transfusion in abdominal aortic aneurysm patients altered the balance of transcription gene expression of TH1, TH2, TH17, and Treg cells.","PeriodicalId":14560,"journal":{"name":"Iranian journal of allergy, asthma, and immunology","volume":"23 1","pages":"107-114"},"PeriodicalIF":1.5,"publicationDate":"2024-02-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140131449","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A Quality-of-life Study in Patients with Anaphylaxis to Hymenoptera Venom in Iran. 伊朗膜翅目昆虫毒液过敏性休克患者生活质量研究

IF 1.5 4区医学

Iranian journal of allergy, asthma, and immunology Pub Date : 2024-02-11 DOI: 10.18502/ijaai.v23i1.14954

Mohammad Hasan Bemaniyan, Maryam Heidari, Marzieh Tavakol, Mohammad Nabavi, Fatemeh Ramezani Kashal, Maryam Gholami, Bita Bemaniyan

{"title":"A Quality-of-life Study in Patients with Anaphylaxis to Hymenoptera Venom in Iran.","authors":"Mohammad Hasan Bemaniyan, Maryam Heidari, Marzieh Tavakol, Mohammad Nabavi, Fatemeh Ramezani Kashal, Maryam Gholami, Bita Bemaniyan","doi":"10.18502/ijaai.v23i1.14954","DOIUrl":"10.18502/ijaai.v23i1.14954","url":null,"abstract":"Little is known about the quality of life of patients with anaphylaxis to Hymenoptera venom. The Vespid Allergy Quality of Life Questionnaire (VQLQ) is commonly used to assess the psychological burden of this condition. This study aimed to evaluate the validity and reliability of the Persian version of VQLQ. In this cross-sectional study, VQLQ was translated into Persian according to expert recommendations. The final translated version of VQLQ was then administered to 115 patients with Hymenoptera venom allergy at an asthma and allergy clinic in Iran. More than half of the participants were between 20 and 40 years of age, and 60% were male. Fear, anxiety, and outdoor activities had the most significant impact on the quality of life of patients with Hymenoptera venom allergy. Additionally, quality of life was more affected in women than in men, while no correlation was found with age. Furthermore, the quality of life was affected by a history of acute anaphylactic shock due to Hymenoptera venom. The Persian version of VQLQ enables the measurement of quality of life in patients with Hymenoptera venom allergy in the Iranian population. The inclusion of VQLQ in the initial evaluation of these patients may potentially guide allergist in providing support for venom-specific immunotherapy.","PeriodicalId":14560,"journal":{"name":"Iranian journal of allergy, asthma, and immunology","volume":"23 1","pages":"59-68"},"PeriodicalIF":1.5,"publicationDate":"2024-02-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140131409","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Measurement of the Neutrophils Count and Oxidative Burst in Neutrophils of Patients with Sanjad Sakati Syndrome. 桑贾德-萨卡提综合征患者中性粒细胞计数和氧化爆发的测定

IF 1.5 4区医学

Iranian journal of allergy, asthma, and immunology Pub Date : 2024-02-11 DOI: 10.18502/ijaai.v23i1.14959

Farhad Abolnezhadian, Majid Aminzadeh, Sara Iranparast, Sajad Dehnavi, Fatemeh Dousti, Moosa Sharifat, Hamid Moradzadegan

{"title":"Measurement of the Neutrophils Count and Oxidative Burst in Neutrophils of Patients with Sanjad Sakati Syndrome.","authors":"Farhad Abolnezhadian, Majid Aminzadeh, Sara Iranparast, Sajad Dehnavi, Fatemeh Dousti, Moosa Sharifat, Hamid Moradzadegan","doi":"10.18502/ijaai.v23i1.14959","DOIUrl":"10.18502/ijaai.v23i1.14959","url":null,"abstract":"Sanjad Sakati Syndrome (SSS) is categorized as a neuroendocrine-related disease due to disorders of the nervous and hormonal systems. Since hormonal changes in these patients may affect the nature and function of the immune system. Thus, in this study, cell count and phagocytotic function of neutrophils were evaluated which may be influenced by changes in the hormonal rate and growth factors. In this study, the neutrophil count value and the oxidative burst were evaluated in six patients diagnosed with SSS and six healthy individuals. There was a significant reduction in the neutrophil count observed in SSS patients compared to healthy controls (37.41±7.93 percent vs. 66.5±6.8 percent). However, there was no significant difference in neutrophil oxidative index between patients with SSS and control subjects (172.33±55.08 vs. 217.00±77.38). We concluded that in patients with SSS, the phagocytic activity of neutrophils was not affected by hormonal changes, while the number of neutrophils and neutrophil-to-lymphocyte ratio (NLR) index were decreased.","PeriodicalId":14560,"journal":{"name":"Iranian journal of allergy, asthma, and immunology","volume":"23 1","pages":"115-121"},"PeriodicalIF":1.5,"publicationDate":"2024-02-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140131415","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The Role of Innate and Adaptive Immune System in the Pathogenesis of Schizophrenia. 先天性和适应性免疫系统在精神分裂症发病机制中的作用。

IF 1.5 4区医学

Iranian journal of allergy, asthma, and immunology Pub Date : 2024-02-11 DOI: 10.18502/ijaai.v23i1.14951

Marziyeh Soltani, Yousef Mirzaei, Ali Hussein Mer, Mina Mohammad-Rezaei, Zahra Shafaghat, Soheila Fattahi, Fatemeh Azadegan-Dehkordi, Meghdad Abdollahpour-Alitappeh, Nader Bagheri

{"title":"The Role of Innate and Adaptive Immune System in the Pathogenesis of Schizophrenia.","authors":"Marziyeh Soltani, Yousef Mirzaei, Ali Hussein Mer, Mina Mohammad-Rezaei, Zahra Shafaghat, Soheila Fattahi, Fatemeh Azadegan-Dehkordi, Meghdad Abdollahpour-Alitappeh, Nader Bagheri","doi":"10.18502/ijaai.v23i1.14951","DOIUrl":"10.18502/ijaai.v23i1.14951","url":null,"abstract":"Schizophrenia is one of the most severely debilitating mental disorders that affects 1.1% of the world's population. The exact cause of the disease is not known, but genetics, environmental factors (such as infectious agents, season and region of birth, exposure to viruses, low birth weight, advanced paternal age, and tobacco), and immune system dysfunction can all contribute to the development of schizophrenia. Recently, the role of the immune system in schizophrenia has received much attention. Both acquired and innate immune systems are involved in the pathogenesis of schizophrenia and facilitate the disease's progression. Almost all cells of the immune system including microglia, B cells, and T cells play an important role in the blood-brain barrier damage, inflammation, and in the progression of this disease. In schizophrenia, the integrity of the blood-brain barrier is reduced and then the immune cells are recruited into the endothelium following an increase in the expression of cell adhesion molecules. The entry of immune cells and cytokines leads to inflammation and antibody production in the brain. Accordingly, the results of this study strengthen the hypothesis that the innate and acquired immune systems are involved in the pathogenesis of schizophrenia.","PeriodicalId":14560,"journal":{"name":"Iranian journal of allergy, asthma, and immunology","volume":"23 1","pages":"1-28"},"PeriodicalIF":1.5,"publicationDate":"2024-02-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140131416","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Immunomodulatory Effect of Calcitriol on Experimental Autoimmune Encephalomyelitis Mice, A Multiple Sclerosis Animal Model 骨化三醇对实验性自身免疫性脑脊髓炎小鼠(多发性硬化症动物模型)的免疫调节作用

4区医学

Iranian journal of allergy, asthma, and immunology Pub Date : 2023-11-07 DOI: 10.18502/ijaai.v22i5.13995

Behrouz Robat-Jazi, Mona Oraei, Sama Bitarafan, Seyed Alireza Mesbah-Namin, Ali Noori-Zadeh, Fatemeh Mansouri, Karim Parastouei, Ali Anissian, Mir Saeed Yekaninejad, Ali Akbar Saboor-Yaraghi

{"title":"Immunomodulatory Effect of Calcitriol on Experimental Autoimmune Encephalomyelitis Mice, A Multiple Sclerosis Animal Model","authors":"Behrouz Robat-Jazi, Mona Oraei, Sama Bitarafan, Seyed Alireza Mesbah-Namin, Ali Noori-Zadeh, Fatemeh Mansouri, Karim Parastouei, Ali Anissian, Mir Saeed Yekaninejad, Ali Akbar Saboor-Yaraghi","doi":"10.18502/ijaai.v22i5.13995","DOIUrl":"https://doi.org/10.18502/ijaai.v22i5.13995","url":null,"abstract":"Previous studies noted an imbalance in T helper (Th) 17 and regulatory T cells (Tregs) in experimental autoimmune encephalomyelitis (EAE), a multiple sclerosis animal model. calcitriol, vitamin D's active form, was found to ameliorate EAE symptoms by favoring Tregss over Th17 cells, suggesting immunomodulatory effects. This study aimed to assess calcitriol's impact on EAE manifestations and cytokine profile in mice. In this study, we recruited twenty-eight C57BL/6 mice and divided them into 4 groups: healthy controls, EAE, EAE with calcitriol treatment, and healthy mice with calcitriol treatment. CD4+ T cells were isolated from splenocytes using magnetic-activated cell sorting. Real-time polymerase chain reaction was employed to quantify the genes associated with Th9 cells (i.e., SPI1 encoding PU.1 and IL9 encoding interleukin [IL]-9). Moreover, the levels of IL-17 and transforming growth factor beta (TGF-β) were evaluated through enzyme-linked immunosorbent assay in the supernatant of CD4+ T cell culture stimulated by anti-CD3 and anti-CD28 antibodies for 72 hours. In the supernatant of CD4+ T cell cultures, the levels of interleukin-17 (IL-17) were significantly increased, while the levels of transforming growth factor beta (TGF-β) were decreased in the EAE Group compared to the healthy control group. Calcitriol treatment reversed these changes and attenuated EAE symptoms, as confirmed in hematoxylin and eosin, and luxol fast blue stains. Notably, calcitriol increased IL9 gene expression in both EAE and healthy mice. This study provides further evidence of the anti-inflammatory effects of calcitriol and its role in attenuating EAE.","PeriodicalId":14560,"journal":{"name":"Iranian journal of allergy, asthma, and immunology","volume":"72 6","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-11-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135475684","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Amygdalin Improves Allergic Asthma via the Thymic Stromal Lymphopoietin–dendritic Cell–OX40 Ligand Axis in a Mouse Model 小鼠模型中苦杏仁苷通过胸腺基质淋巴生成素-树突状细胞ox40配体轴改善过敏性哮喘

4区医学

Iranian journal of allergy, asthma, and immunology Pub Date : 2023-11-07 DOI: 10.18502/ijaai.v22i5.13993

Wen Cui, Huan Zhou, Ya-zun Liu, Yan Yang, Yi-zhong Hu, Zhao-peng Han, Jian-er Yu, Zheng Xue

{"title":"Amygdalin Improves Allergic Asthma via the Thymic Stromal Lymphopoietin–dendritic Cell–OX40 Ligand Axis in a Mouse Model","authors":"Wen Cui, Huan Zhou, Ya-zun Liu, Yan Yang, Yi-zhong Hu, Zhao-peng Han, Jian-er Yu, Zheng Xue","doi":"10.18502/ijaai.v22i5.13993","DOIUrl":"https://doi.org/10.18502/ijaai.v22i5.13993","url":null,"abstract":"Asthma, characterized by persistent inflammation and increased sensitivity of the airway, is the most common chronic condition among children. Novel, safe, and reliable treatment strategies are the focus of current research on pediatric asthma. Amygdalin, mainly present in bitter almonds, has anti-inflammatory and immunoregulatory potential, but its effect on asthma remains uninvestigated. Here, the impact of amygdalin on the thymic stromal lymphopoietin (TSLP)–dendritic cell (DC)–OX40L axis was investigated. A BALB/c mouse model for allergic asthma was established using the ovalbumin-sensitization method. Amygdalin treatment was administered between days 21 and 27 of the protocol. Cell numbers and hematoxylin and eosin (H&E) staining in bronchoalveolar lavage fluid (BALF) were used to observe the impact of amygdalin on airway inflammation. TSLP, IL-4, IL-5, IL-13, and IFN-γ concentrations were determined via Enzyme-linked immunosorbent assay (ELISA). TSLP, GATA-3, and T-bet proteins were measured using western blotting. Cell-surface receptor expression on DCs (MHC II, CD80, and CD86) was assessed via flow cytometry. OX40L mRNA and protein levels were detected using western blotting and qRT-PCR, respectively. Amygdalin treatment attenuated airway inflammation decreased BALF TSLP levels, inhibited DC maturation, restrained TSLP-induced DC surface marker expression (MHCII, CD80, and CD86), and further decreased OX40L levels in activated DCs. This occurred together with decreased Th2 cytokine levels (IL-4, IL-5, and IL-13) and GATA3 expression, whereas Th1 cytokine (IFN-γ) levels and T-bet expression increased. Amygdalin thus regulates the Th1/Th2 balance through the TSLP–DC–OX40L axis to participate in inflammation development in the airways, providing a basis for potential allergic asthma treatments.","PeriodicalId":14560,"journal":{"name":"Iranian journal of allergy, asthma, and immunology","volume":"72 5","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-11-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135475685","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Combined Treatment of Progressive Encephalitis in an X-linked Agammaglobulinemia Patient x连锁无球蛋白血症患者进行性脑炎的综合治疗

4区医学

Iranian journal of allergy, asthma, and immunology Pub Date : 2023-11-07 DOI: 10.18502/ijaai.v22i5.13999

Marco Antonio Yamazaki-Nakashimada, Patricia Herrera-Mora, Alfonso Mahrx-Bracho, Gabriela López-Herrera, Juan Carlos Bustamante-Ogando, Selma Cecilia Scheffler-Mendoza

引用次数: 0

MicroRNA-29a-3p Accelerates Inflammatory Damage in Neonatal Pneumonia Via Targeting Krüppel-like Factor 4 MicroRNA-29a-3p通过靶向kr<s:1> ppel样因子4加速新生儿肺炎的炎症损伤

4区医学

Iranian journal of allergy, asthma, and immunology Pub Date : 2023-11-07 DOI: 10.18502/ijaai.v22i5.13994

Xiao Juan Xu, Wei Liu, ShiNa Liland

{"title":"MicroRNA-29a-3p Accelerates Inflammatory Damage in Neonatal Pneumonia Via Targeting Krüppel-like Factor 4","authors":"Xiao Juan Xu, Wei Liu, ShiNa Liland","doi":"10.18502/ijaai.v22i5.13994","DOIUrl":"https://doi.org/10.18502/ijaai.v22i5.13994","url":null,"abstract":"Neonatal pneumonia (NP) is a frequently occurring illness during the neonatal phase. The study investigated the molecular process and the role of microRNA (miR)-29a-3p in NP. Peripheral blood was collected from NP patients and healthy newborns. Human lung fibroblasts cell line (WI-38) were treated with lipopolysaccharide (LPS)) to establish a cellular model for NP. Then, miR-29a-3p and Krüppel-like Factor 4 (KLF4) levels were detected by RT-qPCR or Western blot. The relationship between miR-29a-3p and KLF4 was confirmed by dual luciferase reporter gene assay. Cell survival was assessed using the CCK-8 assay, whereas the levels of interleukin-6, tumor necrosis factor-α, and IL-1β were quantified using ELISA. Additionally, apoptosis was evaluated through flow cytometry. Meanwhile, Bax and Bcl-2 were detected by RT-qPCR. Neonatal rats were administered LPS intraperitoneally (3 mg/kg) to induce NP, and pathological injury and inflammatory reaction were analyzed. MiR-29a-3p was elevated but KLF4 was silenced in NP patient’s serum, LPS-treated WI-38 cell line, and LPS-treated newborn rats. Silence of miR-29a-3p or elevation of KLF4 constrained cell proliferation with inflammation of LPS-treated WI-38 cell line. MiR-29a-3p immediately targeted KLF4. Additionally, silence of miR-29a-3p alleviated LPS-stimulated lung injury and inflammation in neonatal rats. The protective action of silenced miR-29a-3p in LPS-treated WI-38 cell line and newborn rats was turned around by silencing KLF4. This study demonstrates originally that miR-29a-3p boosts inflammatory damage in NP via targeting KLF4, offering a basis for clinically diagnosing and treating NP.","PeriodicalId":14560,"journal":{"name":"Iranian journal of allergy, asthma, and immunology","volume":"69 2","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-11-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135476706","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Cycle Threshold Values Predict COVID-19 Severity and Mortality but are not Correlated with Laboratory Markers 周期阈值预测COVID-19严重程度和死亡率，但与实验室标志物无关

4区医学

Iranian journal of allergy, asthma, and immunology Pub Date : 2023-11-07 DOI: 10.18502/ijaai.v22i5.13996

Behnaz Esmaeili, Hoda Khoshnevis, Atefe Alirezaee, Abbas Shakoori, Zahra Pourpak, Hamid Chegini, Zahra Ahmadinejad

{"title":"Cycle Threshold Values Predict COVID-19 Severity and Mortality but are not Correlated with Laboratory Markers","authors":"Behnaz Esmaeili, Hoda Khoshnevis, Atefe Alirezaee, Abbas Shakoori, Zahra Pourpak, Hamid Chegini, Zahra Ahmadinejad","doi":"10.18502/ijaai.v22i5.13996","DOIUrl":"https://doi.org/10.18502/ijaai.v22i5.13996","url":null,"abstract":"Many studies have evaluated the possible utility of cycle threshold (Ct) values as a predictor of Coronavirus disease 2019 (COVID-19) severity and patient outcome. Given the inconsistent results, we aimed to evaluate the association between severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) Ct values and disease severity, inflammatory markers, and outcomes in Iranian patients with COVID-19. A retrospective study of 528 patients with COVID-19 hospitalized from September 2020 to October 2021 was conducted. Demographic, clinical, and laboratory data of patients were retrieved from electronic medical records. Ct values were analyzed as a continuous variable after subcategorizing into 3 groups: low (Ct values<20), medium (Ct values 20 to 30), and high (Ct values>30). Of the 528 patients (45.1% female) aged 13 to 97 years, 109 patients had low Ct values, 312 patients had medium, and 107 patients had high Ct values. Patients with low Ct values were more likely to present with critical COVID-19, require invasive mechanical ventilation and develop complications such as acute respiratory distress syndrome and pneumonia. Furthermore, patients with low or medium Ct values were more likely to die compared to patients with high Ct values.Multivariate analysis showed that patients with low or medium Ct values were more likely to have severe COVID-19 compared with patients with high Ct values. The multivariate analysis also showed a higher risk of mortality in patients with low Ct values compared to patients with high Ct values, although this was not statistically significant. Our findings revealed that Ct values were an independent predictor of COVID-19 severity. The risk of mortality was higher in patients with low Ct values. However, further investigation is needed to address the correlation between Ct values and inflammatory factors.","PeriodicalId":14560,"journal":{"name":"Iranian journal of allergy, asthma, and immunology","volume":"70 3","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-11-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135476702","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0