Japanese circulation journal最新文献_第5页

Transient ventricular wall thickening in acute myocarditis: a serial echocardiographic and histopathologic study. 急性心肌炎的一过性心室壁增厚:一系列超声心动图和组织病理学研究。

Japanese circulation journal Pub Date : 2001-09-20 DOI: 10.1253/JCJ.65.863

S. Hiramitsu, S. Morimoto, S. Kato, A. Uemura, N. Kubo, K. Kimura, A. Sugiura, T. Itoh, H. Hishida

{"title":"Transient ventricular wall thickening in acute myocarditis: a serial echocardiographic and histopathologic study.","authors":"S. Hiramitsu, S. Morimoto, S. Kato, A. Uemura, N. Kubo, K. Kimura, A. Sugiura, T. Itoh, H. Hishida","doi":"10.1253/JCJ.65.863","DOIUrl":"https://doi.org/10.1253/JCJ.65.863","url":null,"abstract":"The present study was designed to determine whether the wall thickening seen in acute myocarditis is caused by interstitial edema. The study group comprised 25 patients (idiopathic myocarditis, 17; eosinophilic myocarditis, 8) in whom acute myocarditis was diagnosed histologically and who underwent echocardiography and endomyocardial biopsy during both the acute and convalescent phases. The following echocardiographic parameters were measured: interventricular septum and left ventricular posterior wall thickness, left ventricular end-diastolic dimension, and left ventricular ejection fraction. Based on the myocardial biopsy specimens, the degree of interstitial edema was classified into 3 grades [(-), 1(+), 2(+)] and the transverse diameter of cardiac myocytes was measured using light microscopy. The thickness of both the interventricular septum and left ventricular wall decreased from 14.3+/-3.7 mm and 13.3+/-2.4 mm in the acute phase to 9.7+/-1.7 mm (p<0.001) and 10.2+/-1.7 mm (p<0.0001), respectively, in the convalescent phase. Edema was present in 22 patients (88.0%) in the acute phase, but in the convalescent phase, edema was present in only 7 patients (28.0%), indicating a significant reduction in the degree of edema (p<0.0001). Cardiac myocyte diameter did not differ significantly between the acute (13.6+/-1.1 microm) and convalescent (13.8+/-1.8 microm) phases.","PeriodicalId":14544,"journal":{"name":"Japanese circulation journal","volume":"65 1","pages":"863-6"},"PeriodicalIF":0.0,"publicationDate":"2001-09-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"80944044","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 88

Multiple pulmonary emboli with pulmonary hypertension caused by effort thrombosis and effective balloon venoplasty of the subclavian vein. 锁骨下静脉球囊成形术致肺动脉高压的多发性肺栓塞。

Japanese circulation journal Pub Date : 2001-09-20 DOI: 10.1253/JCJ.65.920

N. Isaka, N. Yamada, S. Araki, K. Onishi, Munenobu Motoyasu, T. Okinaka, Masaaki Ito, Takeshi Nakano

引用次数: 6

Hypertrophic cardiomyopathy with midventricular obstruction and apical aneurysm: a case report. 肥厚性心肌病合并室中梗阻及顶动脉瘤1例。

Japanese circulation journal Pub Date : 2001-09-20 DOI: 10.1253/JCJ.65.915

K. Harada, T. Shimizu, Y. Sugishita, A. Yao, J. Suzuki, K. Takenaka, Y. Hirata, R. Nagai, T. Takahashi

{"title":"Hypertrophic cardiomyopathy with midventricular obstruction and apical aneurysm: a case report.","authors":"K. Harada, T. Shimizu, Y. Sugishita, A. Yao, J. Suzuki, K. Takenaka, Y. Hirata, R. Nagai, T. Takahashi","doi":"10.1253/JCJ.65.915","DOIUrl":"https://doi.org/10.1253/JCJ.65.915","url":null,"abstract":"A 71-year-old woman had hypertrophic cardiomyopathy associated with midventricular obstruction and an apical aneurysm in the left ventricle. She had had abnormal electrocardiograms for more than 30 years and for the past year had been suffering from occasional attacks of dizziness and low systemic blood pressure. Holter 24-h electrocardiographic monitoring revealed ventricular paroxysmal contractions (676/day) with nonsustained ventricular tachycardia. Doppler echocardiography revealed paradoxical jet flow from the apical aneurysm to the left ventricular outflow during early diastole. Magnetic resonance imaging depicted midventricular hypertrophy and a dyskinetic thin apical wall, which were confirmed by angiography. Coronary angiograms showed no narrowing of the major extramural coronary arteries, but there was compression of aberrant coronary arteries apparently feeding the hypertrophic portion of the left ventricular wall. Stress thallium-201 myocardial imaging showed a persistent severe defect in the left ventricular apex. A hemodynamic study revealed low cardiac output and an intraventricular pressure gradient (approximately 90 mmHg) between the left ventricular apical high-pressure chamber and the subaortic low-pressure chamber. The present case represents a rare combination of hypertrophic cardiomyopathy, midventricular obstruction, and an apical aneurysm in an elderly woman. Myocardial ischemia may have played an important role in the genesis of the apical aneurysm.","PeriodicalId":14544,"journal":{"name":"Japanese circulation journal","volume":"64 1","pages":"915-9"},"PeriodicalIF":0.0,"publicationDate":"2001-09-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"81429039","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 34

Characterization of anti-myocardial autoantibodies in Japanese patients with dilated cardiomyopathy. 日本扩张型心肌病患者抗心肌自身抗体的特征。

Japanese circulation journal Pub Date : 2001-09-20 DOI: 10.1253/JCJ.65.867

A. Baba, T. Yoshikawa, M. Chino, A. Murayama, K. Mitani, S. Nakagawa, I. Fujii, M. Shimada, M. Akaishi, S. Iwanaga, Y. Asakura, K. Fukuda, H. Mitamura, S. Ogawa

{"title":"Characterization of anti-myocardial autoantibodies in Japanese patients with dilated cardiomyopathy.","authors":"A. Baba, T. Yoshikawa, M. Chino, A. Murayama, K. Mitani, S. Nakagawa, I. Fujii, M. Shimada, M. Akaishi, S. Iwanaga, Y. Asakura, K. Fukuda, H. Mitamura, S. Ogawa","doi":"10.1253/JCJ.65.867","DOIUrl":"https://doi.org/10.1253/JCJ.65.867","url":null,"abstract":"Few previous reports have comprehensively screened all the anti-myocardial autoantibodies (AMCA) in relation to other clinical profiles in patients with idiopathic dilated cardiomyopathy (IDC), so the present study used both immunohistochemistry (FITC) and immunoblotting (IB) for screening patients with IDC in order to characterize the clinical significance of AMCA. Sera were collected from 100 patients with IDC and age-matched 100 healthy control subjects (CTL). For FITC, an unfixed frozen section of human myocardium was used for the standard indirect immunofluorescence; for IB, total cardiac homogenates of the same myocardium were blotted to serum at 2 sets of dilution (1:200 and 1:10,000). The positive rates of AMCA detection for each method were as follows (IDC vs CTL); 39% vs 6% for FITC, 38% vs 4% for IB (1:200), and 10% vs 0% for IB (1:10,000). Fifty-nine patients with IDC and 8 CTL were positive for AMCA by either method, and 18 patients with IDC and 2 CTL were positive for AMCA by both methods. IB-positivity at 1:200 was an independent predictor by multiple logistic regression analysis of non-sustained ventricular tachycardias as well as left ventricular end-diastolic diameter and plasma norepinephrine concentration.","PeriodicalId":14544,"journal":{"name":"Japanese circulation journal","volume":"136 1","pages":"867-73"},"PeriodicalIF":0.0,"publicationDate":"2001-09-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"86307651","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 15

Role of transesophageal echocardiography in the prediction of thromboembolism in patients with chronic nonvalvular atrial fibrillation. 经食管超声心动图在预测慢性非瓣膜性房颤患者血栓栓塞中的作用。

Japanese circulation journal Pub Date : 2001-09-20 DOI: 10.1253/JCJ.65.874

S. Miyazaki, Takahide E. Ito, Michihiro E. Suwa, Tomomi Nakamura, A. Kobashi, Y. Kitaura

{"title":"Role of transesophageal echocardiography in the prediction of thromboembolism in patients with chronic nonvalvular atrial fibrillation.","authors":"S. Miyazaki, Takahide E. Ito, Michihiro E. Suwa, Tomomi Nakamura, A. Kobashi, Y. Kitaura","doi":"10.1253/JCJ.65.874","DOIUrl":"https://doi.org/10.1253/JCJ.65.874","url":null,"abstract":"The purpose of this study was to determine whether parameters derived from transesophageal echocardiography (TEE) could predict thromboembolism in patients with chronic nonvalvular atrial fibrillation (AF). Eighty-nine patients, mean age 66+/-9 years, who underwent TEE in 1996 to 1999 were studied. The clinical endpoint was a thromboembolic event, including transient ischemic attack (TIA). Sixty-seven patients (75%) were anticoagulated with warfarin after TEE. After a follow-up period of 29+/-10 months, 1 patient died suddenly, 4 had a thromboembolism, and 3 had a TIA; the annual embolic event rate was 3.3%. Left atrial appendage (LAA) thrombus (86% vs 17%, p<0.001), LAA dysfunction (LAA velocity <20 cm/s; 71% vs 25%, p=0.009), and severe LA spontaneous echo contrast (29% vs 2%, p=0.002) were more prevalent in patients with an embolic event than in those without. In patients with LAA thrombus, the annual event rate was 11% as compared with 1.2% in those without (p=0.004). On the Cox proportional hazards model analysis, LAA thrombus (chi-square 7.0, p=0.008), severe LA spontaneous echo contrast (chi-square 7.0, p=0.008), and LAA dysfunction (chi-square 5.9, p=0.015) were significantly related to thromboembolism. Multivariate analysis revealed that LAA thrombus (chi-square 5.5, p=0.019) and LAA dysfunction (chi-square 4.0, p=0.045) were the independent predictors. In conclusion, TEE parameters, particularly the presence of LAA thrombus, can be used to assess thromboembolic potential in patients with chronic nonvalvular AF.","PeriodicalId":14544,"journal":{"name":"Japanese circulation journal","volume":"770 1","pages":"874-8"},"PeriodicalIF":0.0,"publicationDate":"2001-09-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"89502126","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 21

Relationship between blood pressure and cardiac events in patients with a healed myocardial infarction. 心肌梗死愈合后患者血压与心脏事件的关系

Japanese circulation journal Pub Date : 2001-09-20 DOI: 10.1253/JCJ.65.879

M. Wufuer, K. Ishikawa, T. Takenaka, A. Kimura, Takahiro Hayashi, K. Kanamasa

{"title":"Relationship between blood pressure and cardiac events in patients with a healed myocardial infarction.","authors":"M. Wufuer, K. Ishikawa, T. Takenaka, A. Kimura, Takahiro Hayashi, K. Kanamasa","doi":"10.1253/JCJ.65.879","DOIUrl":"https://doi.org/10.1253/JCJ.65.879","url":null,"abstract":"This study investigated the association between blood pressure (diastolic [DBP] and systolic [SBP]) and cardiac events in a total of 6,602 patients with a healed myocardial infarction (MI) (5,320 men, 1,282 women; mean age, 58.9+/-10.4 years), including in-patients and out-patients, from January 1986 to January 1999. The primary endpoints (cardiac events) were recurrent MI (fatal and non-fatal), death from congestive heart failure, or sudden death. The total number of cardiac events was 195 (3.0%) and the incidences of the 3 cardiac events were compared among the 3 DBP groups (DBP low group, <70 mmHg; DBP middle group, 70-89 mmHg; DBP high group, > or =90 mmHg). There were some significant differences in the characteristics of patients among the 3 groups, but no significant difference in cardiac death and total mortality was found among the 3 groups. Cardiac events occurred in 59 of 1,780 patients (3.3%, 33.9/1,000 person years) in the DBP low group, tended to be reduced in number in the DBP middle group (87 of 3,549 patients 2.5%, 21.4/1,000 person years) and were slightly increased in number in the DBP high group (24 of 642 patients 3.7%, 35.2/1,000 person years). Although this correlation between BP and cardiac events appears to be J-shaped, because there is no statistically significant difference in the rate of cardiac events among the 3 groups, we conclude that DBP does not influence the occurrence of cardiac events. The prevalence of cardiac events tended to be greater with a SBP less than 120 mmHg.","PeriodicalId":14544,"journal":{"name":"Japanese circulation journal","volume":"45 1","pages":"879-86"},"PeriodicalIF":0.0,"publicationDate":"2001-09-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"82491754","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 2

Monophasic action potentials of the right atrium in patients with paroxysmal atrial fibrillation. 阵发性心房颤动患者右心房单相动作电位。

Japanese circulation journal Pub Date : 2001-09-20 DOI: 10.1253/JCJ.65.893

J. Sekiya, Y. Ohnishi, T. Inoue, M. Yokoyama

{"title":"Monophasic action potentials of the right atrium in patients with paroxysmal atrial fibrillation.","authors":"J. Sekiya, Y. Ohnishi, T. Inoue, M. Yokoyama","doi":"10.1253/JCJ.65.893","DOIUrl":"https://doi.org/10.1253/JCJ.65.893","url":null,"abstract":"To investigate the mechanism of atrial fibrillation (AF), monophasic action potentials (MAPs) from the atrial myocardium were studied in 7 patients with paroxysmal AF (PAF) and in 7 control individuals. The MAPs were recorded using a contact catheter during sinus rhythm and continuous pacing at the high right atrium (HRA) with pacing cycle lengths of 600, 500 and 400 ms. MAPs were obtained from 6 sites in each participant. The MAPD90 was measured from onset to 90% of MAP repolarization. Average, maximal and minimal MAPD90 (avMAPD90, maxMAPD90 and minMAPD90) were obtained from all participants. The dispersion of MAPD90 (dispMAPD90) was defined as the difference between maxMAPD90 and minMAPD90. The width of each atrial potential (WAP) and the wavelength index (WLI=MAPD90/WAP) were determined. Average, maximal and minimal WLI (avWLI, maxWLI and minWLI) were obtained from all participants. The avMAPD90 and maxMAPD90 did not significantly differ between the 2 groups. The minMAPD90 in the PAF group was significantly smaller than that in the control group at HRA pacing with cycle lengths of 500 and 400 ms (210+/-18ms vs 245+/-14 ms, p<0.05; 207+/-23 ms vs 238+/-20 ms, p<0.05; respectively). The dispMAPD90 was significantly longer in the PAF group than in the control group during sinus and HRA pacing. The WAP value did not differ between the 2 groups. The minWLI in the PAF group was significantly smaller than that in the control group at HRA pacing with cycle lengths of 500 and 400 ms (3.3+/-0.5 vs 3.8+/-0.3, p<0.05; 3.2+/-0.4 vs 3.7+/-0.3, p<0.02). A shortened and widened dispersion of atrial refractoriness may play an important role in the genesis of AF. Furthermore, smaller wavelengths may form in the atrium of patients with PAF.","PeriodicalId":14544,"journal":{"name":"Japanese circulation journal","volume":"12 1","pages":"893-6"},"PeriodicalIF":0.0,"publicationDate":"2001-09-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"80876589","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 7

The DD genotype of angiotensin converting enzyme polymorphism is a risk factor for coronary artery disease and coronary stent restenosis in Japanese patients. 血管紧张素转换酶多态性的DD基因型是日本患者冠状动脉疾病和冠状动脉支架再狭窄的危险因素。

Japanese circulation journal Pub Date : 2001-09-20 DOI: 10.1253/JCJ.65.897

I. Taniguchi, T. Yamazaki, K. Wagatsuma, T. Kurusu, Y. Shimazu, K. Takikawa, M. Yoshikawa, S. Kageyama, S. Mochizuki

{"title":"The DD genotype of angiotensin converting enzyme polymorphism is a risk factor for coronary artery disease and coronary stent restenosis in Japanese patients.","authors":"I. Taniguchi, T. Yamazaki, K. Wagatsuma, T. Kurusu, Y. Shimazu, K. Takikawa, M. Yoshikawa, S. Kageyama, S. Mochizuki","doi":"10.1253/JCJ.65.897","DOIUrl":"https://doi.org/10.1253/JCJ.65.897","url":null,"abstract":"Stent implantation has decreased the incidence of restenosis after coronary intervention, but has not eliminated it. The contribution of the angiotensin-converting enzyme (ACE) genotype to the development of coronary artery disease and restenosis after coronary stenting was investigated in 67 Japanese patients in whom 103 lesions in which stents had been successfully implanted were assessed by quantitative coronary angiography, before, immediately after coronary stenting, and during follow-up. The distribution of the patients with the DD, ID, and II genotypes was 13%, 54%, and 33%, respectively. The prevalence of multivessel disease in the DD genotype was significantly higher (DD genotype: 78%; ID genotype: 58%; II genotype: 27%, chi2=8.13, p=0.016) and the late loss in the DD genotype (1.43+/-0.96 mm) was significantly greater (ID genotype: 0.78+/-0.98 mm and II genotype: 0.79+/-0.88 mm, p<0.05 vs DD genotype). However, there was no significant difference in the restenosis rate among the 3 genotypes. The present study in Japanese patients indicates that the DD genotype is associated with more extensive coronary artery disease and progression of the inward remodeling within the stented lesion, which is primarily caused by neointimal hyperplasia.","PeriodicalId":14544,"journal":{"name":"Japanese circulation journal","volume":"84 1","pages":"897-900"},"PeriodicalIF":0.0,"publicationDate":"2001-09-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"74608150","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 27

Myocardial inflammatory cell infiltrates in cases of dilated cardiomyopathy as a determinant of outcome following partial left ventriculectomy. 扩张型心肌病患者心肌炎症细胞浸润作为左心室部分切除术后预后的决定因素。

Japanese circulation journal Pub Date : 2001-08-20 DOI: 10.1253/JCJ.65.797

Y. Kanzaki, F. Terasaki, M. Okabe, T. Hayashi, H. Toko, H. Shimomura, S. Fujioka, Y. Kitaura, K. Kawamura, Y. Horii, T. Isomura, H. Suma

{"title":"Myocardial inflammatory cell infiltrates in cases of dilated cardiomyopathy as a determinant of outcome following partial left ventriculectomy.","authors":"Y. Kanzaki, F. Terasaki, M. Okabe, T. Hayashi, H. Toko, H. Shimomura, S. Fujioka, Y. Kitaura, K. Kawamura, Y. Horii, T. Isomura, H. Suma","doi":"10.1253/JCJ.65.797","DOIUrl":"https://doi.org/10.1253/JCJ.65.797","url":null,"abstract":"Partial left ventriculectomy (PLV) can be used to treat refractory congestive heart failure caused by dilated cardiomyopathy (DCM). In order to understand the relationship between the underlying myocardial injury and early clinical outcomes after PLV, histopathologic, immunohistochemical and virologic studies of the resected myocardium were performed. The posterolateral left ventricular walls from 27 patients with idiopathic DCM were examined. Cardiomyocyte diameter, degree of myocardial fibrosis, degree of cardiomyocyte degeneration, and degree of inflammatory cell infiltration were compared with mortality rates. Polymerase chain reaction was performed to detect enterovirus genome in the myocardium. Some patients had inflammatory cell infiltrates with focal accumulations of lymphocytes and macrophages, including both cytotoxic/suppressor T-cells and helper/inducer T-cells. The number of inflammatory cells (activated lymphocytes plus macrophages/mm2) was significantly greater in patients who died of cardiac insufficiency after surgery (27.8 +/- 5.7; n = 7) than in the survivors (11.1 +/- 2.5; n = 15). There was no significant difference in the degree of myocardial fibrosis, cardiomyocyte diameter or degree of cardiomyocyte degeneration between the 2 groups. Enterovirus genome was detected in the myocardium of 9 (38%) of 24 patients examined and 5 of these enterovirus-positive hearts had severe inflammatory cell infiltrates (37.9 +/- 2.5/mm2). Early survival in patients undergoing PLV for DCM is significantly affected by the degree of myocardial inflammation, so patients with more severe or ongoing inflammation may have poor clinical outcomes. Chronic myocarditis may play an important role in the etiology and pathophysiology of idiopathic DCM.","PeriodicalId":14544,"journal":{"name":"Japanese circulation journal","volume":"2 1","pages":"797-802"},"PeriodicalIF":0.0,"publicationDate":"2001-08-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"87985739","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 37

Role of calcineurin in insulin-like growth factor-1-induced hypertrophy of cultured adult rat ventricular myocytes. 钙调磷酸酶在胰岛素样生长因子-1诱导的成年大鼠心室肌细胞肥大中的作用。

Japanese circulation journal Pub Date : 2001-08-20 DOI: 10.1253/JCJ.65.815

T. Miyashita, Yasuchika Takeishi, Hiroki Takahashi, Shuichi Kato, Isao Kubota, H. Tomoike

{"title":"Role of calcineurin in insulin-like growth factor-1-induced hypertrophy of cultured adult rat ventricular myocytes.","authors":"T. Miyashita, Yasuchika Takeishi, Hiroki Takahashi, Shuichi Kato, Isao Kubota, H. Tomoike","doi":"10.1253/JCJ.65.815","DOIUrl":"https://doi.org/10.1253/JCJ.65.815","url":null,"abstract":"The present study examined the role of calcineurin in insulin-like growth factor (IGF)-1-induced hypertrophy in primary cultures of adult rat ventricular myocytes (ARVM), prepared from the ventricles of 14-16-week-old male Sprague-Dawley rats. The effects of several humoral factors, including phenylephrine, angiotensin II, endothelin-1, IGF-1 and interleukin-6, on the morphology of ARVM were studied. Myocyte surface area was significantly increased by IGF-1 (2,268 +/- 571 to 3,018 +/- 836 microm2, p < 0.01), but not by other humoral factors. This hypertrophic effect of IGF-1 was blocked by genistein (tyrosine kinase inhibitor), PD98059 (MEK inhibitor). These findings suggest that IGF-1 produces ARVM hypertrophy by a tyrosine kinase-MEK mediated pathway as has been reported in neonatal cardiomyocytes. IGF-1-mediated ARVM hypertrophy was also attenuated by cyclosporine A (calcineurin inhibitor), and staurosporine and chelerythrine (protein kinase C inhibitors). IGF-1 markedly increased calcineurin activity (8.7 +/- 1.2 to 98.0 +/- 54.3 pmol x h(-1) mg(-1), p < 0.01), and this activation was completely blocked by pre-treatment with cyclosporine A (8.5 +/- 11.4pmol x h(-1) x mg(-1), p < 0.01) and chelerythrine (2.3 +/- 2.7 pmol x h(-1) mg(-1), p < 0.01). It appears that IGF-1 activates calcineurin by a protein kinase C-dependent pathway. Increased mRNA expression of atrial natriuretic factor by IGF-1 was inhibited by cyclosporine A (p < 0.01). The findings indicate that IGF-1 induces ARVM hypertrophy by protein kinase C and calcineurin-related mechanisms. The fact that elevated calcineurin activity and induced atrial natriuretic factor mRNA expression by IGF-1 were blocked by cyclosporine A further supports the hypothesis that calcineurin is critically involved in IGF-1-induced ARVM hypertrophy.","PeriodicalId":14544,"journal":{"name":"Japanese circulation journal","volume":"39 1","pages":"815-9"},"PeriodicalIF":0.0,"publicationDate":"2001-08-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"88582741","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 24