A. Said, U. Hawas, S. El-Shenawy, S. M. Nofal, K. Rashed
{"title":"Flavonoids and some biological activities of Ailanthus excelsa leaves","authors":"A. Said, U. Hawas, S. El-Shenawy, S. M. Nofal, K. Rashed","doi":"10.18478/IUFSJB.48603","DOIUrl":"https://doi.org/10.18478/IUFSJB.48603","url":null,"abstract":"In the present study, the methanolic extract of Ailanthus excelsa (Roxb) leaves, a plant used in Egyptian traditional medicine was investigated for phytochemical constituents and some biological activities. In vitro: antioxidant activity and in vivo: Lethal dose (LD 50 ) acute and chronic toxicity and anti-nociceptive and anti-inflammatory effects were determined. It was found that methanolic extract of Ailanthus excelsa leaves is nontoxic up to 5 g.kg -1 b. wt in mice. Also rats were given a daily single oral dose of the extract for 7 and 30 successive days for assessment of acute and chronic toxicity. The extract did not induce any significant change in serum levels of ALT, AST, GGT, total protein, albumin, BUN and creatinine as compared with saline control group in rats. The extract exhibited a significant antioxidant, anti-nociceptive activity and no anti-inflammatory effects. Chromatographic separation of A. excelsa MeOH extract has yielded eleven known flavonoid compounds. Their structures were established on the basis of chromatographic properties and spectroscopic (UV, 1 H, 13 C NMR, MS) analyses.","PeriodicalId":14521,"journal":{"name":"IUFS Journal of Biology","volume":"1 1","pages":"41-55"},"PeriodicalIF":0.0,"publicationDate":"2010-11-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"85758403","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Morphological Characteristics and Growth Abilities of Free Living Amoeba Isolated From Domestic Tap Water Samples in İstanbul","authors":"Z. Zeybek, Miray Üstüntürk, Ali Rıza Binay","doi":"10.18478/IUFSJB.08200","DOIUrl":"https://doi.org/10.18478/IUFSJB.08200","url":null,"abstract":"Free living amoeba (FLA) have been isolated from water and soil samples throughout the world. Among the many genera of FLA , members of only four genera are responsible for human disease: Acanthamoeba spp., Balamuthia mandrillaris , Naegleria fowleri and Sappinia diploidea . Naegleria spp. and Acanthamoeba spp. can be cultivated on nonnutrient agar (NNA) in the presence of living or dead bacteria. Balamuthia , however, will not grow with bacteria as a food source but they can feed upon smaller soil amoeba. In the current study we try to find out the morphological appearance (Trichrome staining) and growth characteristics of free living amoeba in different liquid culture media previously isolated from domestic tap water samples (10 isolates) and a strain of Acanthamoeba castellanii (A. castellanii) isolated from a keratitis case. Although A.castellanii strain grew on proteose peptone-yeast extract-glucose and proteose peptone-yeast (with or without antibiotics), our isolates from domestic tap water samples did not grow on any tested liquid culture media. It is suggested that investigating both the morphological characteristics and the growth characteristics on liquid culture media are necessary for the identification of free living amoeba.","PeriodicalId":14521,"journal":{"name":"IUFS Journal of Biology","volume":"31 22","pages":"17-23"},"PeriodicalIF":0.0,"publicationDate":"2010-11-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"91404520","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"The Anti-Genotoxic Effect of Taurine on Aluminum Sulphate-Induced DNA Damage in Human Peripheral Lymphocytes","authors":"H. Turkez, F. Geyikoğlu","doi":"10.18478/IUFSJB.01865","DOIUrl":"https://doi.org/10.18478/IUFSJB.01865","url":null,"abstract":"Aluminum sulphate Al 2 (SO 4 ) 3 is commonly used as a coagulant in the purification of drinking water in many regions of the world. Aluminum (Al) is also involved in several pharmaceutical and cosmetic products. However, Al is suspected to cause serious disorders such as Alzheimer's by oxidative DNA damage. On the other hand, taurine (TA) is an amino acid found in mammalian tissues and it has been suggested to play a role in the defense against cellular damage. The objective of this study was to determine whether the supplemetation of a natural antioxidant, TA conferred the protection against Al exposure in human blood cultures. For this aim, sister chromatid exchange (SCE) and micronucleus (MN) rates were assessed. Present results showed that 10 μg/ml of Al 2 (SO 4 ) 3 has no effect on SCE and MN rates, but 20 μg/ml of this compound increased the rates of SCEs and MN. Besides, the TA at all studied concentrations (25, 50 and 100 μg/ml) did not alter the mean SCE and MN values as compared to controls. Moreover, the negative cytogenetic alterations induced by Al could be significantly ( P<0.05 ) reduced by the application of TA. This study reveals for the first time the ameliorative role of TA against Al-induced DNA damage in vitro .","PeriodicalId":14521,"journal":{"name":"IUFS Journal of Biology","volume":"18 1","pages":"25-32"},"PeriodicalIF":0.0,"publicationDate":"2010-11-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"78725330","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Y. Aydin, T. Talas-Oğraş, A. Altinkut, I. Ismailoğlu, E. Arican, N. Gozukirmizi
{"title":"Cytohistological Studies During Cotton Somatic Embryogenesis With Brassinosteroid Application","authors":"Y. Aydin, T. Talas-Oğraş, A. Altinkut, I. Ismailoğlu, E. Arican, N. Gozukirmizi","doi":"10.18478/IUFSJB.01577","DOIUrl":"https://doi.org/10.18478/IUFSJB.01577","url":null,"abstract":"The histology and chromosome alterations of somatic embryo initiation and development in cotton ( Gossypium hirsutum L. cv. Coker) was examined during in vitro exogenous brassinosteroid (BR) application. Calli developed from hypocotyl explants were subjected to 0.5 μM BR. After 10 weeks of calli cultures with somatic embryos at different developmental stages were fixed for histological examinations. The histological examination showed that two types of embryogenic cells induced; epidermal or subepidermal. The embryogenic mass and somatic embryos were mostly derived from several morphologically competent cells from subepidermal cases; however single progenitor cells for epidermal situations. Chromosome behaviour of the cells surrounding embryogenic cells showed abnormal divisions and higher level of polyploidy on BR treated calli when compared to control group.","PeriodicalId":14521,"journal":{"name":"IUFS Journal of Biology","volume":"160 1","pages":"33-39"},"PeriodicalIF":0.0,"publicationDate":"2010-11-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"87903941","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"ENVIRONMENTAL BIOLOGY AND PATHOPHYSIOLOGY OF CADMIUM","authors":"Y. Çotuk, M. Belivermiş, Ö. Kılıç","doi":"10.18478/IUFSJB.63020","DOIUrl":"https://doi.org/10.18478/IUFSJB.63020","url":null,"abstract":"Cadmium (Cd) is a soft, silver-white metal being together with zinc and mercury in group II b in the periodic table. It's melting and boiling point are 320.9 0 C and 765 0 C, respectively. Cd is rapidly oxidized into cadmium oxide in the air. When reactive gases such as carbon dioxide, sulphur dioxide, sulphur trioxide and hydrogen chloride or water vapour are present in the air, cadmium vapour reacts to produce cadmium carbonate, cadmium sulphite, cadmium sulphate, cadmium chloride or cadmium hydroxide. These compounds may be formed in chimneys and emitted into the environment. It is indicated that zinc-bearing coals of the central United States and carboniferous age coals of other countries contain large sub economic resources of cadmium. Although some cadmium compounds such as acetate, chloride and sulphate are soluble in water; cadmium oxide, carbonate and sulphide are insoluble.","PeriodicalId":14521,"journal":{"name":"IUFS Journal of Biology","volume":"33 1","pages":"1-5"},"PeriodicalIF":0.0,"publicationDate":"2010-11-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"89460341","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"EFFECT OF OVERIECTOMY AND OF ESTROGEN ADMINISTRATION UPON DUODENAL ULCERATION INDUCED BY CYSTEAMINE","authors":"A. Kv, M. KuraneM, M. PillaiM.","doi":"10.18478/IUFSJB.75270","DOIUrl":"https://doi.org/10.18478/IUFSJB.75270","url":null,"abstract":"Duodenal ulcers were induced in ovariectomized, intact and old mice using cysteamine hydrochloride. Under these experimental conditions ovariectomy and old age strikingly increased sensitivity to ulcer induction while estrogen administration showed a decrease in sensitivity to ulcer induction. Nevertheless, the administration of estrogen in old mice showed no effects in either intact or overiectomized mice. This change in ulcer sensitivity reflected from histological, histochemical and biochemical studies. The histological study was performed by using haematoxylin-eosin and haematoxyline staining technique. The histochemistry of the duodenal region was studied by using periodic acid Schiff reaction (PAS) for glycoproteins. The biochemical study was performed to study various constituents of glycoproteins like hexose, fucose, sialic acid and the protein. The result showed that ulcer severity was more in overiectomized cysteamine treated mice and old mice treated with cysteamine. The histological studies showed that ovariectomy decreased or did not show any change in the ulcer sensitivity considering cryptus Lieburkuhn and Brunner's glands. The same result reflected by differential intensity in the staining property of the Brunner's gland. The biochemical study showed that the glycoprotein contents were reduced many times in overiectomized cysteamine treated mice and their reversal in estrogen administered ovariectomized cysteamine injected mice. These findings prove that estrogen protects the duodenal ulcer from cysteamine administration.","PeriodicalId":14521,"journal":{"name":"IUFS Journal of Biology","volume":"35 1","pages":"7-16"},"PeriodicalIF":0.0,"publicationDate":"2010-11-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"80005554","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
A. Berrichi, Reda Tazi, Ahmed Belliro, N. Kouddane, A. Bouali
{"title":"Role of salt stress on seed germination and growth of jojoba plant Simmondsia chinensis (Link) Schneider","authors":"A. Berrichi, Reda Tazi, Ahmed Belliro, N. Kouddane, A. Bouali","doi":"10.18478/IUFSJB.01476","DOIUrl":"https://doi.org/10.18478/IUFSJB.01476","url":null,"abstract":"An extensive problem in agriculture is the accumulation of salts from irrigation water. Evaporation and transpiration remove pure water from the soil. This water loss concentrates solutes in the soil. When irrigation water contains a high concentration of solutes, salts can reach rapidly injurious levels vis-a-vis salt-sensitive species. One factor limiting jojoba plant ( Simmondsia chinensis (Link) Schneider) domestication in the oriental areas of Morocco is the damage caused by salt effect since irrigation enhances salinity in plantation soils. The objectives of this study were to define the effect of various NaCl concentration levels on plant growth and seed germination. The tolerance to salinity of jojoba seed and seedlings was assessed at NaCl concentrations of 0, 1, 2, 3, 5, and 7 g l −1 and the experiment was undertaken in the laboratory under greenhouse conditions. Results obtained show that more than half of the seeds germinated in the presence of 7g/l of NaCl; whereas 5g/l of NaCl inhibited completely the emergence of plumules. Concerning jojoba seedlings, 3 g/l of NaCl marked the start of negative affect on the growth.","PeriodicalId":14521,"journal":{"name":"IUFS Journal of Biology","volume":"29 1","pages":"95-102"},"PeriodicalIF":0.0,"publicationDate":"2010-11-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"86996290","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Isolation and identification of Two new cyclic amino acids from the seeds of Zizyphus spina-christi L. (Willd) by means of 1H-NMR-13C-NMR-HSQC-HMBC and GC-MS","authors":"A. Said, A. Huefner, E. Table, G. Fawzy","doi":"10.18478/IUFSJB.70605","DOIUrl":"https://doi.org/10.18478/IUFSJB.70605","url":null,"abstract":"In this study we isolate two new cyclic amino acids from the seeds of Zizyphus spina-christi L. (Willd ) 70% methanolic extract. The two compounds were identified by means of 1 H-NMR- 13 C-NMR-HSQC-HMBC and GC-MS as 4-hydroxymethyl-1-methyl pyrrolidine-2-carboxylic acid (less polar and major compound) and 4-hydroxy-4-hydroxymethyl-1-methyl pyrrolidine-2-carboxylic acid (more polar and minor compound) of the ratio 4:3.","PeriodicalId":14521,"journal":{"name":"IUFS Journal of Biology","volume":"7 1","pages":"77-86"},"PeriodicalIF":0.0,"publicationDate":"2010-11-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"77658199","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"LENGTH-WEIGHT RELATIONSHIPS OF FISHES IN SHALLOW WATERS OF ERDEK BAY (SEA OF MARMARA, TURKEY)","authors":"Çetin Keskin, Ö. Gaygusuz","doi":"10.18478/IUFSJB.34222","DOIUrl":"https://doi.org/10.18478/IUFSJB.34222","url":null,"abstract":"The study was carried out monthly between April 2000 and December 2001 on the seagrass and/or sandy bottom in shallow waters of Erdek Bay (Sea of Marmara, Turkey). Samples were collected using 35 m long beach seine. Fish samples were identified, measured and weighed. The length-weight relationships of juvenile and adult specimens of 36 fish species were investigated. The parameters a and b were calculated by functional regression, as was the coefficient of determination ( r 2 ). r 2 values were higher than 0.950 for 27 species in the list, while only one species was lower than 0.80. The exponent b ranged from 2.282 to 3.741.","PeriodicalId":14521,"journal":{"name":"IUFS Journal of Biology","volume":"5 1","pages":"87-94"},"PeriodicalIF":0.0,"publicationDate":"2010-11-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"89604216","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Benin Toklu Alıçlı, N. Balkıs, A. Toklu, Ahmet Höbek
{"title":"First record of Amphorellopsis tetragona ( Protozoa: Ciliophora: Tintinnina)","authors":"Benin Toklu Alıçlı, N. Balkıs, A. Toklu, Ahmet Höbek","doi":"10.18478/IUFSJB.72728","DOIUrl":"https://doi.org/10.18478/IUFSJB.72728","url":null,"abstract":"Amphorellopsis tetragona was reported for the first time from the Sea of Marmara and contributed to the regional check-list of the plankton species of Turkish seas. The specimen was collected on 17 October 2008 from surface neritic waters with a depth of 30m in the research area. The primary hydrographic parameters, temperature 17 oC, salinity 27 ppt and dissolved oxygen 5 mg l -1 , were recorded at the sampling station.","PeriodicalId":14521,"journal":{"name":"IUFS Journal of Biology","volume":"22 1","pages":"103-107"},"PeriodicalIF":0.0,"publicationDate":"2010-11-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"75588383","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}