IUFS Journal of Biology最新文献

Lipoxygenase Inhibitory Activities of Some Plant Extracts and Chemical Compounds 一些植物提取物和化合物的脂氧合酶抑制活性研究

IUFS Journal of Biology Pub Date : 2016-03-23 DOI: 10.18478/IUFSJB.47797

O. Sacan, Emin Turhan

引用次数: 11

The role of Escherichia coli bacterioferritin in stressed induced conditions 大肠杆菌铁蛋白在应激诱导条件下的作用

IUFS Journal of Biology Pub Date : 2015-01-01 DOI: 10.18478/iufsjb.70302

E. Çelen, M. Kılıç, G. Moore

{"title":"The role of Escherichia coli bacterioferritin in stressed induced conditions","authors":"E. Çelen, M. Kılıç, G. Moore","doi":"10.18478/iufsjb.70302","DOIUrl":"https://doi.org/10.18478/iufsjb.70302","url":null,"abstract":"Escherichia coli possesses at least two iron storage proteins: FtnA (ferritin) and Bfr (Bacterioferritin). FtnA is shown to be a major iron storage protein in E. coli . Although Bfr resembles the ferritin in many of its structural and functional features, the role of Bfr in E. coli is not clear. The aim of this study was to investigate the role of E. coli Bfr in stress induced conditions. In this study, E. coli bfr - (lacking the chromosomal bfr gene) and E. coli bfr + (the same as the former but containing the E. coli bfr overexpression vector) mutants were used. In order to examine the role of E. coli Bfr in stress induced conditions, E. coli cells were grown in the presence of excess iron. In the case of hydrogen peroxide toxicity, the cells were grown in an agar plate containing hydrogen peroxide discs and the results of toxicity were expressed as the size of the cell death zone. In order to find out whether Bfr supports cell growth under iron and phosphate starvation, the E. coli cells were grown in iron and phosphate depleted media. In E. coli the overproduced Bfr did not support the growth of cells under iron and phosphate deficient conditions. The results showed that overproduced Bfr in E. coli does not contribute to the survival of the cell in iron and phosphate starvation. Furthermore, it does not enhance the resistance of E. coli against iron and hydrogen peroxide toxicity.","PeriodicalId":14521,"journal":{"name":"IUFS Journal of Biology","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2015-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"87471158","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Phenolic composition and antibacterial activity of crude methanolic Calendula officinalis flower extract against plant pathogenic bacteria 金盏花粗甲醇提取物的酚类成分及其对植物病原菌的抑菌活性

IUFS Journal of Biology Pub Date : 2015-01-01 DOI: 10.18478/IUFSJB.29216

Özüm Larçin, D. A. Körpe, Ö. Işeri, F. Sahin

{"title":"Phenolic composition and antibacterial activity of crude methanolic Calendula officinalis flower extract against plant pathogenic bacteria","authors":"Özüm Larçin, D. A. Körpe, Ö. Işeri, F. Sahin","doi":"10.18478/IUFSJB.29216","DOIUrl":"https://doi.org/10.18478/IUFSJB.29216","url":null,"abstract":"Plant-derived compounds comprise diverse biological activities with different mechanisms of actions. Aim of this study was to comparatively evaluate antibacterial activities of methanol and aqueous flower and leaf extracts of Calendula officinalis (pot marigold) on plant-borne pathogens, with total phenolic contents and analysis of phenolics. Flower methanol and aqueous extracts had activity against 4 and 5 strains, respectively out of 11 phytopathogens tested. Highest activity was obtained with methanol extract of flower against E. amylovara and C. michiganensis in all strains tested with 256 and 512 µg/mL minimum inhibitory concentrations, respectively. Total phenolic content of flower extracts were higher than leaf extracts, and the methanol extract of the flower had the highest total phenols among four extracts obtained. Two flower extracts with antibacterial activity were tested for phenolic content. Chlorogenic acid, caffeic acid, rutin, and salicylic acid were common in both methanol and aqueous flower extracts. Gallic acid was only present in methanol extract, whereas vanillic acid was present in the aqueous extract. Higher antibacterial activity of the methanol extract may be correlated to the considerably higher relative rutin amount. Considering eco-safety and effectiveness, antibacterial activities of plant extracts would be important in phytopathogen control.","PeriodicalId":14521,"journal":{"name":"IUFS Journal of Biology","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2015-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"87816773","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 7

Three wheat cultivars’ responses to NaCl treatments: changes in lipid peroxidation, cell viability, hydrogen peroxide content and antioxidant defence parameters 3个小麦品种对NaCl处理的响应:脂质过氧化、细胞活力、过氧化氢含量和抗氧化防御参数的变化

IUFS Journal of Biology Pub Date : 2015-01-01 DOI: 10.18478/IUFSJB.56960

A. Sen, S. Alikamanoğlu

{"title":"Three wheat cultivars’ responses to NaCl treatments: changes in lipid peroxidation, cell viability, hydrogen peroxide content and antioxidant defence parameters","authors":"A. Sen, S. Alikamanoğlu","doi":"10.18478/IUFSJB.56960","DOIUrl":"https://doi.org/10.18478/IUFSJB.56960","url":null,"abstract":"The responses of lipid peroxidation, antioxidant defence parameters, the content of hydrogen peroxide (H 2 O 2 ) and cell viability were investigated in callus cultures of Triticum aestivum L. (wheat) ‘Tekirdag’, ‘Pehlivan’ and ‘Flamura-85’ cultivars under various NaCl concentrations (0, 0.05, 0.1 and 0.15 M). According to two-way ANOVA, the interaction between NaCl treatments and cultivars on the antioxidant enzyme activity and malondialdehyde (MDA), proline and H 2 O 2 content were statistically significant. Statistically significant differences between cultivars were also detected using two-way ANOVA analyses of antioxidant enzyme activities, MDA and H 2 O 2 content. The activity of antioxidant enzymes such as superoxide dismutase (SOD), guaiacol peroxidase (POX), catalase (CAT), ascorbate peroxidase (APX) and glutathione reductase (GR) increased under salt treatments, except for catalase activity in Tekirdag. Proline and oxidized glutathione (GSSG) content increased significantly with NaCl concentrations whereas the reduced glutathione (GSH) increase was not statistically significant. GSH/GSSG ratios decreased under salt treatments in all three wheat cultivars as compared to the control. MDA and H 2 O 2 content increased, but the viabilities of callus cultures decreased as salt concentrations increased. Based on the cellular responses of three different wheat calli against salinity, Flamure-85 was the best one because of its superior antioxidant defence system.","PeriodicalId":14521,"journal":{"name":"IUFS Journal of Biology","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2015-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"77190234","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Cytogenetic Studies in Some Species of Medicago L. in Iran 伊朗部分苜蓿属植物的细胞遗传学研究

IUFS Journal of Biology Pub Date : 2014-11-27 DOI: 10.18478/IUFSJB.63904

S. Sadeghian, S. M. H. Hejazi

引用次数: 6

Scale Surface Microstructure and Scale Size in Three Mugilid Fishes (Teleostei, Mugilidae) of Iran from Three Different Habitats 伊朗三种不同生境木鱼的鳞片表面微观结构和鳞片大小

IUFS Journal of Biology Pub Date : 2014-11-27 DOI: 10.18478/IUFSJB.62393

H. Esmaeili, R. Khaefi, G. Sayyadzadeh, M. S. Tahami, Benafsheh Parsi, A. Gholamifard

{"title":"Scale Surface Microstructure and Scale Size in Three Mugilid Fishes (Teleostei, Mugilidae) of Iran from Three Different Habitats","authors":"H. Esmaeili, R. Khaefi, G. Sayyadzadeh, M. S. Tahami, Benafsheh Parsi, A. Gholamifard","doi":"10.18478/IUFSJB.62393","DOIUrl":"https://doi.org/10.18478/IUFSJB.62393","url":null,"abstract":"A substantial number of species within mugilid family has been recorded from marine, brackish and freshwater bodies of Iran. Descriptions of these species have been based mainly on morphological and anatomical data; however, the characters related to the fish scales have not up to now been intensively studied and employed for the identification of the fish species. The objective of this study is to test whether (i) scale surface morphology and microstructure, and (ii) scale size can be used to discriminate species. To achieve these objectives, scales of three species of the genus Liza from three different habitats, Liza abu (freshwater, Maharlu basin), Liza klunzingeri (sea, Persian Gulf) and Liza saliens (brackish water, Caspian Sea) have been studied using SEM images, scale measurements, and uni and multivariate statistics. It is opined that scale surface morphology and microstructure may help in distinguishing the species. In addition, scale size and J-indices, represent a valuable tool for species separation, which corroborates earlier studies for the use of these indices in fish taxonomy.","PeriodicalId":14521,"journal":{"name":"IUFS Journal of Biology","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2014-11-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"73221935","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 13

Lactic Acid Production by Lactobacillus brevis Isolated from Oral Microbiota 从口腔微生物群中分离的短乳杆菌产乳酸

IUFS Journal of Biology Pub Date : 2014-11-27 DOI: 10.18478/IUFSJB.65510

Yağmur Toptaş, G. Akça, A. Çabuk

引用次数: 2

Poly (DL-Lactic-Co-Glycolic Acid) Microparticle- Doxorubicin Formulations for Anti-cancer Drug Delivery 用于抗癌药物传递的聚乳酸-羟基乙酸微粒子-阿霉素制剂

IUFS Journal of Biology Pub Date : 2014-11-27 DOI: 10.18478/IUFSJB.31212

G. Çağlar, S. Yalçın, G. Gündüz, U. Gündüz

{"title":"Poly (DL-Lactic-Co-Glycolic Acid) Microparticle- Doxorubicin Formulations for Anti-cancer Drug Delivery","authors":"G. Çağlar, S. Yalçın, G. Gündüz, U. Gündüz","doi":"10.18478/IUFSJB.31212","DOIUrl":"https://doi.org/10.18478/IUFSJB.31212","url":null,"abstract":"Cancer is a group of diseases in which normal cells are converted to cells capable of autonomous growth and invasion. In the chemotherapeutic control of cancer, drugs are usually given systemically so they reach toxic levels in cancer cells. This causes serious side effects in healthy cells. Another important problem with chemotherapy is resistance developed to cytotoxic drugs (multi drug resistance). As a possible solution to these problems, in the present study, two different microparticle fabrication methods were compared; double emulsion solvent evaporation (SE) method for encapsulation of Doxorubicin into PLGA microparticles. The most appropriate method was the SE techniquies which lead to higher encapsulation efficiencies. Processing factors were evaluated for their effects on encapsulation efficiency and results indicated that any change that hinder drug diffusion would result in increased drug content in microparticles. To asses the cytotoxicities of synthesized microparticles, cell proliferation assays were performed with XTT reagent on Doxorubicin resitant and sensitive breast cancer cell lines, MCF-7. DOX entrapped microparticles was effective on both sensitive and DOX resistant MCF-7 cells. The concentration of drug in resistant cancer cells was increased indicating a partial reversal of drug resistance. The results of this study will provide new insights to the development of new drug delivery systems for cancer therapy.","PeriodicalId":14521,"journal":{"name":"IUFS Journal of Biology","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2014-11-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"82538488","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 2

A Study on Floristic Composition, Chorology and Ecological Structure: A case Study from a Small-scale Forest Reserve, Talesh, Iran 植物区系组成、年代与生态结构研究——以伊朗Talesh小规模森林保护区为例

IUFS Journal of Biology Pub Date : 2014-11-27 DOI: 10.18478/IUFSJB.59206

M. Ravanbakhsh, T. Amini

{"title":"A Study on Floristic Composition, Chorology and Ecological Structure: A case Study from a Small-scale Forest Reserve, Talesh, Iran","authors":"M. Ravanbakhsh, T. Amini","doi":"10.18478/IUFSJB.59206","DOIUrl":"https://doi.org/10.18478/IUFSJB.59206","url":null,"abstract":"This study, carried out on vegetation of the Gisoum forest reserve with 171 hectares area located 14 km to Resvanshar city, Guilan province, was to examine the structure and composition of the forest vegetation. A first topographic map of the area with the scale of 1:10000 was prepared and the study area was delimited. Transect- quadrate method of sampling was used to record the floristic and phytosociological data necessary for the analysis in about 44 quadrates of 400 m2. Some vegetation parameters viz. density and diameter at breast height (DBH) for trees (over story vegetation) as well as cover-abundance for under story vegetation were recorded, respectively. The life form spectrum of Raunkiaer, the dominant vegetation type of the area, is based on importance value and vegetation structure based on frequency classes, also their chorology caring a central importance in vegetation description were determined. The study of the floristic list showed that 76 species belonging to 66 genera and 45 families existed in the area.The largest families were Asteraceae and Rosaceae with 5 species. Investigation of the geographical distribution of plant species indicated that 44 % belonged to the Europe – Siberian zone. Plant life forms by Raunkiaer method showed that phanerophytes with 35.5 % and hemicryptophytes with 27.6 % were the most frequent life forms. Dominant vegetation type based on importance value index was Buxus hyrcanus-Parrotia persica. Also the evaluation of vegetation structure based on frequency class revealed that abundance-distribution was irregular in under story and regular in upper story vegetation.","PeriodicalId":14521,"journal":{"name":"IUFS Journal of Biology","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2014-11-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"74033656","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 3

Programmed Cell Death in the Digestive Canal of Bombyx mori (Lepidoptera: Bombycidae) during Prepupal Period 家蚕(鳞翅目:家蚕科)蛹前消化道细胞程序性死亡

IUFS Journal of Biology Pub Date : 2014-01-01 DOI: 10.18478/IUFSJB.28196

Gözde Selek, E. Goncu, O. Parlak

引用次数: 1