Lactic Acid Production by Lactobacillus brevis Isolated from Oral Microbiota

Abstract

Lactic acid is used in various industrial areas such as the food, pharmaceutical, textile and other chemical industries. In this study, 49 lactic acid bacteria were isolated from oral microbiota of voluntary patients. These isolates were screened for their lactic acid production abilities. The isolate which has a high potential for lactic acid production was selected. The optimal conditions for lactic acid production by Lactobacillus brevis were determined. According to the results obtained, sucrose as carbon source, 50 g/L of sucrose amount, diammonium hydrogen citrate as nitrogen source, 8 g/L of nitrogen amount, 42 °C as temperature, 4.5 x 108 CFU/mL of inoculum amount and 72 hours of incubation time were found to be optimal values.