大肠杆菌铁蛋白在应激诱导条件下的作用

E. Çelen, M. Kılıç, G. Moore
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引用次数: 0

摘要

大肠杆菌拥有至少两种铁储存蛋白:FtnA(铁蛋白)和Bfr(细菌铁蛋白)。FtnA被证明是大肠杆菌中主要的铁储存蛋白。虽然Bfr在许多结构和功能特征上与铁蛋白相似,但Bfr在大肠杆菌中的作用尚不清楚。本研究的目的是探讨大肠杆菌Bfr在应激诱导条件下的作用。本研究采用大肠杆菌bfr -(缺失染色体bfr基因)和大肠杆菌bfr +(与前者相同,但含有大肠杆菌bfr过表达载体)突变体。为了检验大肠杆菌Bfr在应激诱导条件下的作用,大肠杆菌细胞在过量铁的存在下生长。在过氧化氢毒性的情况下,将细胞生长在含有过氧化氢圆盘的琼脂板中,毒性结果以细胞死亡区的大小表示。为了确定Bfr是否支持铁和磷酸盐饥饿下的细胞生长,我们在缺铁和缺磷培养基中培养大肠杆菌细胞。在大肠杆菌中,过量产生的Bfr不支持铁和磷酸盐缺乏条件下的细胞生长。结果表明,在铁磷酸盐饥饿条件下,大肠杆菌中过量产生Bfr并不能促进细胞的存活。此外,它不会增强大肠杆菌对铁和过氧化氢毒性的抵抗力。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
The role of Escherichia coli bacterioferritin in stressed induced conditions
Escherichia coli possesses at least two iron storage proteins: FtnA (ferritin) and Bfr (Bacterioferritin). FtnA is shown to be a major iron storage protein in E. coli . Although Bfr resembles the ferritin in many of its structural and functional features, the role of Bfr in E. coli is not clear. The aim of this study was to investigate the role of E. coli Bfr in stress induced conditions. In this study, E. coli bfr - (lacking the chromosomal bfr gene) and E. coli bfr + (the same as the former but containing the E. coli bfr overexpression vector) mutants were used. In order to examine the role of E. coli Bfr in stress induced conditions, E. coli cells were grown in the presence of excess iron. In the case of hydrogen peroxide toxicity, the cells were grown in an agar plate containing hydrogen peroxide discs and the results of toxicity were expressed as the size of the cell death zone. In order to find out whether Bfr supports cell growth under iron and phosphate starvation, the E. coli cells were grown in iron and phosphate depleted media. In E. coli the overproduced Bfr did not support the growth of cells under iron and phosphate deficient conditions. The results showed that overproduced Bfr in E. coli does not contribute to the survival of the cell in iron and phosphate starvation. Furthermore, it does not enhance the resistance of E. coli against iron and hydrogen peroxide toxicity.
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