IUFS Journal of Biology最新文献_第5页

The role of insulin-like growth factor-I and polyamines in developing rat small intestine 胰岛素样生长因子- 1和多胺在发育大鼠小肠中的作用

IUFS Journal of Biology Pub Date : 2011-10-27 DOI: 10.18478/IUFSJB.99580

O. Karabulut-Bulan, Ş. Bolkent

{"title":"The role of insulin-like growth factor-I and polyamines in developing rat small intestine","authors":"O. Karabulut-Bulan, Ş. Bolkent","doi":"10.18478/IUFSJB.99580","DOIUrl":"https://doi.org/10.18478/IUFSJB.99580","url":null,"abstract":"IGF-I, Insulin like growth factor-I is a multi-functional polypeptide which organizes the proliferation and differentiation of various cell types and has a metabolic activity similar to insulin. We know that IGF-I receptors localize in the gastrointestinal tract. Polyamines play an important role in the gastrointestinal growth which connect with IGF-I. DFMO, I±-difluoromethylornithine is an irreversible inhibitor of ODC, ornithine decarboxylase, the rate-limiting enzyme in the synthesis of polyamines. In the IGF-I-promoted growth the role of polyamines mainly investigate in vitro systems, however, in vivo studies are necessary to clarify the subject. Our aim in this study is to examine the in vivo role of IGF-I as a stimulant in the growth of developing rat's small intestine, and to clarify the role of polyamines in the period of growth promoted by IGF-I. In this study, animals were divided into 2 groups. DFMO (500 mg/kg/day) was administered for 10 days to 9 rats forming the experimental group. Sterile 0.9% NaCl was injected at the same volume as for the experimental animals into 8 rats forming the control group. Tissue samples were taken from the rats for histological, immunohistochemical and biochemical analyses on the 10th day. In the group given DFMO, the expansion and compression of the villi, as well as a decrease in the PAS-positive reaction intensity of brush border and in the number of goblet cells were observed. mmunohistochemical assessments showed that the number of PCNA, ODC and IGF-I positive cells in the DFMO-treated group decreased significantly, compared with the control group. Biochemical investigations showed that DNA values in the small intestinal tissue decreased insignificantly in the DFMO treated group as compared to the control one. We concluded that application of DFMO in the developing rat small intestine inhibited polyamine synthesis and IGF-I synthesis and partially prevented growth of the intestine. \u0000Keywords: Polyamine, I±-difluoromethylornithine, insulin-like growth factor-I, ornithine decarboxylase","PeriodicalId":14521,"journal":{"name":"IUFS Journal of Biology","volume":"89 1","pages":"13-24"},"PeriodicalIF":0.0,"publicationDate":"2011-10-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"83864963","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Morpho-anatomical observations on Colchicum boissieri Orph. in 秋水仙的形态解剖观察。在

IUFS Journal of Biology Pub Date : 2011-10-27 DOI: 10.18478/IUFSJB.35892

E. Sevgi, Orhan Küçüker

{"title":"Morpho-anatomical observations on Colchicum boissieri Orph. in","authors":"E. Sevgi, Orhan Küçüker","doi":"10.18478/IUFSJB.35892","DOIUrl":"https://doi.org/10.18478/IUFSJB.35892","url":null,"abstract":"In this study, the morpho-anatomical features of Colchicum boissieri Orph. were demonstrated. The morphological characteristics of the corm, leaf, flower, fruit and seeds were presented and the habitus of the species were given by photographs. Corm lengths, cataphyll color and length, leaf color, outer-inner tepal dimension (length x width), perigonium tube dimension, outer-inner filament (length) and anther (length), styles color, seed number, shape, dimension and color were determined. The corm of C. boissieri is soboliferous, occasionally tooth-like projections, vertical, soboles horizontal, 2.3-4.5 cm in length and 0.6-1.4 cm in width. The neck (collum) of the corm is 3-5.5 cm in length. The cataphyll is yellowish-white in color, 3-7.5 cm in length. Perigonium tube is 4.4-13 cm in length. The seeds are that wrinkled surface subglobose to globose in shape, yellowish-brown in color, and (1.3-) 1.4-1.9 mm in diameter. \u0000Anatomical characteristics of the corm, leaf, fruit and seeds were shown with photographs. Transverse sections from the middle portion of the corm, leaf, pericarp and seed testa were obtained. The upper and lower surface views of the leaf, pericarp and the seed testa were examined. Anatomical features of C. boissieri were discussed in this research for the first time. Anatomical features of C. boissieri are similar to some Colchicum. In the anatomical view of leaf, it has been determined that there are differences in the number of spongy parenchyma cell layers between the species. Testa of the seeds include epidermis, parenchyma and pigment layer cells. \u0000Key words: Colchicum, morphology, anatomy, soboliferous","PeriodicalId":14521,"journal":{"name":"IUFS Journal of Biology","volume":"06 1","pages":"53-61"},"PeriodicalIF":0.0,"publicationDate":"2011-10-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"86188405","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 3

Length-weight relationship of eight decapod crustaceans of the Sığacık Bay, Aegean Sea coast of Turkey 土耳其爱琴海沿岸Sığacık湾八种十足甲壳类动物的长度-重量关系

IUFS Journal of Biology Pub Date : 2011-06-03 DOI: 10.18478/IUFSJB.17950

Tahir Özcan, T. Katağan

引用次数: 4

Phenolic compounds from seeds of Zizyphus spina-christi 水仙种子中酚类化合物的研究

IUFS Journal of Biology Pub Date : 2011-06-03 DOI: 10.18478/IUFSJB.00166

A. Said, A. Huefner, El-Sayed Ali Abu tabl, G. Fawzy

引用次数: 1

Effects of glucagon-like peptide-2 on TNF-alpha/actinomycin D-induced intestinal epithelial injury: a scanning electron microscopic and immunohistochemical study 胰高血糖素样肽-2对tnf - α /放线菌素d诱导的肠上皮损伤的影响:扫描电镜和免疫组织化学研究

IUFS Journal of Biology Pub Date : 2011-06-03 DOI: 10.18478/IUFSJB.91609

P. A. Pirincci, P. Turan, S. Arbak, Ş. Bolkent

{"title":"Effects of glucagon-like peptide-2 on TNF-alpha/actinomycin D-induced intestinal epithelial injury: a scanning electron microscopic and immunohistochemical study","authors":"P. A. Pirincci, P. Turan, S. Arbak, Ş. Bolkent","doi":"10.18478/IUFSJB.91609","DOIUrl":"https://doi.org/10.18478/IUFSJB.91609","url":null,"abstract":"Glucagon-like peptide-2 (GLP-2) is a peptide hormone with intestinotrophic activity, which is released from the intestinal endocrine cells. The aim of this study was to investigate the effects observed by scanning electron microscope of GLP-2 in intestinal epithelial injury induced by tumor necrosis factor-alpha (TNF-α)/actinomycin D (Act D). In addition, it aimed to elucidate whether the action mechanism of GLP-2 might be mediated by gastrointestinal hormones such as cholecystokinin and somatostatin . The intestinal epithelial injury was induced by intraperitoneal administration of 15 µg/kg TNF-α and 800 µg/kg Act D per mouse. Animals were injected subcutaneously 200 µg/kg GLP-2 analogue every 12 hr for 10 consecutive days prior to the administration of TNF-α and Act D. Scanning electron microscopic examination revealed severe epithelial damage in the small intestine of TNF-α/Act D-administered mice. The administration of TNF-α/Act D was significantly increased the number of somatostatin -immunoreactive endocrine cells, but did not affect the number of cholecystokinin -immunoreactive endocrine cells in the intestinal mucosa . On the other hand, GLP-2 analogue pretreatment prevented TNF-α/Act D-induced intestinal epithelial injury by causing a marked decrease in the degenerative changes and the number of somatostatin -immunoreactive endocrine cells, and a significant increase in the number of cholecystokinin -immunoreactive endocrine cells. As a result, the present study indicates that GLP-2 has a protective effect against TNF-α/Act D-induced intestinal epithelial injury. Morever protective effect of GLP-2 might be related to somatostatin and cholecystokinin.","PeriodicalId":14521,"journal":{"name":"IUFS Journal of Biology","volume":"80 1","pages":"27-38"},"PeriodicalIF":0.0,"publicationDate":"2011-06-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"79987246","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Detection of genetically modified rice: Collaborative validation study of a PCR based detection of genetically modified rice Oryza sativa commercially available in Saudi Arabia 转基因水稻的检测:沙特阿拉伯市售转基因水稻Oryza sativa PCR检测的协同验证研究

IUFS Journal of Biology Pub Date : 2011-06-03 DOI: 10.18478/IUFSJB.73474

M. Ibrahim, I. Alaraidh, A. Amid, A. Farouk, S. Bazaid, R. Greiner, Abdullah Alghunaim

引用次数: 3

The paraldehyde fuchsin positive material in corpora cardiaca of Pimpla turionellae L. (Hymenoptera:Ichneumonidae) during egg maturation 膜翅目:姬蜂科)卵成熟过程中心体三聚甲醛品红阳性物质的研究

IUFS Journal of Biology Pub Date : 2011-06-03 DOI: 10.18478/IUFSJB.49924

Aydin Ozluk, N. Gül

{"title":"The paraldehyde fuchsin positive material in corpora cardiaca of Pimpla turionellae L. (Hymenoptera:Ichneumonidae) during egg maturation","authors":"Aydin Ozluk, N. Gül","doi":"10.18478/IUFSJB.49924","DOIUrl":"https://doi.org/10.18478/IUFSJB.49924","url":null,"abstract":"The purpose of this study is to examine the effects of the neurosecretory material in corpus cardiacum (CC) on the reproduction of endoparasitic Pimpla turionellae L. For this purpose, the egg maturation and amount of the neurosecretory material in corpus cardiacum of the insects were examined by the serial cross sections. Egg maturation was determined by measuring the terminal oocyte length in the serial cross sections of the ovariole. The egg growth which has observed on the 3 rd day, reached the maximum on 15 th day and after that day the egg laying was observed. The sections of insect brains were stained by paraldehyde fuchsin . The amount of the paraldehyde fuchsin positive neurosecretory (PF + NS) material in CC was at a certain level in the first day of egg development. While terminal oocyte was reaching the maximum length, the amount of the PF + NS material in CC decreased to the minimum level. During the egg laying phase, the amount of the PF + NS material in CC reached the maximum level. These observations give us the idea that the neurosecretory material in corpus cardiacum of this insect may be related to the egg development.","PeriodicalId":14521,"journal":{"name":"IUFS Journal of Biology","volume":"25 1","pages":"19-25"},"PeriodicalIF":0.0,"publicationDate":"2011-06-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"84064403","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Trace element concentrations in animal feed samples from Thrace region, Turkey 土耳其色雷斯地区动物饲料样品中的微量元素含量

IUFS Journal of Biology Pub Date : 2011-06-03 DOI: 10.18478/IUFSJB.41340

M. Belivermiş, Ö. Kılıç, T. Engizek, S. Ergenç, A. Çayır

引用次数: 3

The apoptotic effects of Streptozotocin in different dose and administration time on pancreatic islet cells of rats 链脲佐菌素不同剂量和给药时间对大鼠胰岛细胞凋亡的影响

IUFS Journal of Biology Pub Date : 2011-01-01 DOI: 10.18478/IUFSJB.23700

S. Gezginci-Oktayoglu

{"title":"The apoptotic effects of Streptozotocin in different dose and administration time on pancreatic islet cells of rats","authors":"S. Gezginci-Oktayoglu","doi":"10.18478/IUFSJB.23700","DOIUrl":"https://doi.org/10.18478/IUFSJB.23700","url":null,"abstract":"The loss of islet cells by apoptotic cell death plays a central role in the pathogenesis of experimental animal models such as hyperglycemia and diabetes. Streptozotocin (STZ) is widely used for the induction of diabetes in animals by destruction of pancreatic i¢ cells. The aim of the present study is to determine the effects of different dose and administration time of STZ on pancreatic islet cells. With this aim, four different experimental groups consist of the animals treated with citrate buffer (0.01 M, pH: 4.5) only, the animals sacrificed after 5 hours of the 40 mg/kg STZ injection, the animals sacrificed after 6 hours of the 60 mg/kg STZ injection, and the animals sacrificed after 4 hours of the 75mg/kg STZ injection were composed. While the apoptotic cells were observed by TUNEL staining method and according to morphologic criteria of the cells, insulin synthesized cells were shown by immunohistochemical technique. As the result it was determined an increase in blood glucose levels and apoptotic islet cells in 40 and 75 mg/kg STZ-injected rats while islet and beta cell areas were decreased in all groups. These results indicate that 40 and 75 mg/kg STZ injection at the end of 5 and 4 hours, respectively, causes hyperglycemia by triggering apoptosis in islet cells of adult rats. Keywords: Apoptosis, hyperglycemia, islet cell, rat, streptozotocin.","PeriodicalId":14521,"journal":{"name":"IUFS Journal of Biology","volume":"9 1","pages":"43-52"},"PeriodicalIF":0.0,"publicationDate":"2011-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"78628486","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 2

Histological and Morphological Studies of digestive tube and liver of the Persian tooth-carp, Aphanius persicus(Actinopterygii: Cyprinodontidae) 波斯齿鱼(Aphanius persicus)消化道和肝脏的组织学形态学研究(放线翼目:棘齿鱼科)

IUFS Journal of Biology Pub Date : 2010-11-25 DOI: 10.18478/IUFSJB.06589

M. Monsefi, Z. Gholami, H. Esmaeili

引用次数: 27