IUFS Journal of Biology最新文献_第4页

Study of biofilm associated bacteria on polyvinyl chloride, stainless steel and glass surfaces in a model cooling tower system with different microbiological methods 用不同微生物学方法研究模型冷却塔系统中聚氯乙烯、不锈钢和玻璃表面生物膜相关细菌

IUFS Journal of Biology Pub Date : 2012-10-05 DOI: 10.18478/IUFSJB.93915

Nazmiye Ozlem Sanli Yurudu

引用次数: 5

Antioxidant and antibacterial properties of a lichen species Diploschistes scruposus (Schreb.) Norman 一种地衣的抗氧化和抗菌特性诺曼

IUFS Journal of Biology Pub Date : 2012-10-05 DOI: 10.18478/IUFSJB.47020

B. Sökmen, Sinem Aydın, K. Kınalıoğlu

{"title":"Antioxidant and antibacterial properties of a lichen species Diploschistes scruposus (Schreb.) Norman","authors":"B. Sökmen, Sinem Aydın, K. Kınalıoğlu","doi":"10.18478/IUFSJB.47020","DOIUrl":"https://doi.org/10.18478/IUFSJB.47020","url":null,"abstract":"Bu calismada, Diploschistes scruposus (Schreb.) Norman (Graphidaceae) likeninin antibakteriyal ve antioksidan ozellikleri arastirilmistir. D. scruposus likeninin etanol ekstresinin antioksidan aktivitesi bes farkli metodla calisilmistir: DPPH (2,2-difenil-1-pikrilhidrazil) radikal supurme aktivitesi, ABTS.+ (2,2'-azino-bis (3etilbenzotiyazolin-6-sulfonik asit) radikali supurme aktivitesi, indirgeme gucu ve toplam fenolik iceriginin belirlenmesi. Test ekstresinin degisik antioksidan aktiviteleri bilinen antioksidanlarla kiyaslanmistir. Liken ekstresinin %50 inhibisyon konsantrasyon degerleri (IC50), DPPH radikali supurme aktivitesi icin 54.37±0.71; ABTS.+ radikali supurme aktivitesi icin 45.45±0.80 olarak gozlenmistir. D. scruposus etanol ekstresinin 1 mg’inda 161.4±0.01 µg/mL gallik asit esdegeri toplam fenolik bilesikleri bulunmaktadir. Liken ekstresinin antibakteriyal aktivitesi agar kuyu difuzyon yontemine gore belirlenmistir. Maksimum antibakteriyal aktivite Bacillus subtilis’e, en dusuk antibakteriyal aktivite ise Escherichia coli’ye karsi gozlenmistir. Bu calisma, D. scruposus likeninin dogal antioksidan ve antibakteriyal kaynagi olarak kullanilabilecegini gostermistir","PeriodicalId":14521,"journal":{"name":"IUFS Journal of Biology","volume":"1 1","pages":"43-51"},"PeriodicalIF":0.0,"publicationDate":"2012-10-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"89116989","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 8

Ecdysone receptor B1 in Bombyx mori L. (Lepidoptera:Bombycidae) prothoracic gland under various organ culture conditions 不同器官培养条件下家蚕前胸腺蜕皮激素受体B1的研究

IUFS Journal of Biology Pub Date : 2012-10-05 DOI: 10.18478/IUFSJB.06808

Emre Batır, E. Goncu, O. Parlak

{"title":"Ecdysone receptor B1 in Bombyx mori L. (Lepidoptera:Bombycidae) prothoracic gland under various organ culture conditions","authors":"Emre Batır, E. Goncu, O. Parlak","doi":"10.18478/IUFSJB.06808","DOIUrl":"https://doi.org/10.18478/IUFSJB.06808","url":null,"abstract":"During the early last larval instar, hormone secretory activity of prothoracic glands in Bombyx mori L. is undetectable despite the high prothoracicotropic hormone (PTTH) level in hemolymph. Early reports indicated that the inhibition of gland activity is caused by 20-hydroxyecdysteroid (20E) peak on day 2 of the fourth larval instar and juvenile hormone (JH) only resumes this inactive period during the early days of 5th larval instar. Following these reports, prothoracic glands also become a target of its hormone, 20E. Actions of 20E are mediated via heterodimeric ecdysone receptor complex which consists of ecdysone receptor (EcR) and its partner molecule, ultraspiracle (USP). This study was designed to determine the expression pattern of ecdysone receptor B1 (EcR-B1) in the prothoracic glands of B. mori during last larval instar and early pupal stage. Organ culture experiments were carried out to clarify the effects of juvenile hormone and 20-hydroxyecdysone on the EcR-B1 expression. Immunocytochemical studies revealed the presence of EcR-B1 in prothoracic gland cells. A rise of EcR-B1 immoreactivity was detected on day 3 of 5th instar in which glands recover the secretion activity. Juvenile hormone administration to culture medium maintained EcR-B1 immunoreactivity especially on day 6 and day 9 when compared with control but in contrast to this result, an ecdysone application to culture medium weakened the immunoreactivity. These results suggested that negative feedback effect of ecdysone on secretory activity of the glands may be exerted by means of ecdysone receptor and it may also involve consistent and regular 20E secretion during the fifth instar.","PeriodicalId":14521,"journal":{"name":"IUFS Journal of Biology","volume":"86 1","pages":"31-42"},"PeriodicalIF":0.0,"publicationDate":"2012-10-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"79110353","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Antitumor activity of Ailanthus excelsa (Roxb) stem bark fractions and Canthin-6-one 臭椿茎皮部位和cantin -6- 1的抗肿瘤活性

IUFS Journal of Biology Pub Date : 2012-10-05 DOI: 10.18478/IUFSJB.60321

A. Said, K. Rashed, H. Tokuda, A. Huefner

引用次数: 2

The effect of aminoguanidine on the kidney of diabetic albino Balb/c mice 氨基胍对糖尿病白化Balb/c小鼠肾脏的影响

IUFS Journal of Biology Pub Date : 2012-10-05 DOI: 10.18478/IUFSJB.89070

Ebru Gurel, N. Yilmazer, Cihan Demirci-Tansel

{"title":"The effect of aminoguanidine on the kidney of diabetic albino Balb/c mice","authors":"Ebru Gurel, N. Yilmazer, Cihan Demirci-Tansel","doi":"10.18478/IUFSJB.89070","DOIUrl":"https://doi.org/10.18478/IUFSJB.89070","url":null,"abstract":"The aim of this study is to find out how activated inducible nitric oxide synthase (iNOS) and nitric oxide (NO) affect kidney tissue in streptozotocin (STZ)-induced diabetic mice and whether its influence can be prevented by aminoguanidine (AG), a specific iNOS inhibitor. Twenty-four male mice were divided into four study groups (n=6) receiving a daily dose of 100 mg.kg-1 AG for 90 days (Group AG), a single dose of 150 mg.kg-1 STZ (Group STZ), a single dose of 150 mg.kg-1 STZ followed by daily administration of 100 mg.kg-1 AG for 90 days (Group STZ-AG), and intraperitoneally injections of saline only (Group Control) for 90 days. Dispersion of NADPH-diaphorase (NADPH-d) was stronger in the kidney sections of STZ-treated animals compared with the controls. STZ treatment caused disruption of continuity of the brush borders in proximal tubules, glomerular endothelial damage, and considerable renin granules in the juxtaglomerular cells. AG administration following STZ treatment partly prevented histological and cytological changes in kidney cortex, and renin dispersion was similar to that in control animals. We found that increased inducible nitric oxide (iNO) caused kidney tissue degeneration that could be prevented to some extent by AG treatment. There is a possible relationship between increased iNOS and dispersion of renin granules in juxtaglomerular cells in diabetes.","PeriodicalId":14521,"journal":{"name":"IUFS Journal of Biology","volume":"30 1","pages":"17-29"},"PeriodicalIF":0.0,"publicationDate":"2012-10-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"73461632","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 2

Quality of drinking water of Canakkale Province, Turkey 土耳其恰纳卡莱省饮用水质量

IUFS Journal of Biology Pub Date : 2012-06-27 DOI: 10.18478/IUFSJB.07608

M. Coşkun, A. Çayır, M. Coşkun, Aslı Kovanci

引用次数: 1

Cathepsin B inhibition by Z-FA.FMK blocks TNF-a/D-GalN-induced oxidative damage through increasing antioxidant defence in the brain of mice Z-FA抑制组织蛋白酶B。FMK通过增加小鼠大脑的抗氧化防御来阻断TNF-a/ d - galn诱导的氧化损伤

IUFS Journal of Biology Pub Date : 2012-01-01 DOI: 10.18478/IUFSJB.79872

S. Gezginci-Oktayoglu, Ismet Burcu Turkyilmaz, R. Yanardag, Ş. Bolkent

{"title":"Cathepsin B inhibition by Z-FA.FMK blocks TNF-a/D-GalN-induced oxidative damage through increasing antioxidant defence in the brain of mice","authors":"S. Gezginci-Oktayoglu, Ismet Burcu Turkyilmaz, R. Yanardag, Ş. Bolkent","doi":"10.18478/IUFSJB.79872","DOIUrl":"https://doi.org/10.18478/IUFSJB.79872","url":null,"abstract":"Cathepsin B is a cysteine lysosomal protease which takes place in many inflammatory diseases. Inflammatory processes within the brain represent a potential pathogenetic factor in neurodegenerative diseases and inhibiton of cathepsin B, which is an inflammation-related enzyme, can be a potential therapeutic utility in neuroinflammatory diseases. Thus, we researched the effect of benzyloxycarbonyl-phenilalaninealanine fluoromethylketone (Z-FA.FMK), which is a pharmacological inhibitor of cathepsin B, on tumour necrosis factor-I± (TNF-I±) and D-galactosamine (D-GalN)-induced brain damage. Because oxidative damage accompanies inflammatory processes we aimed to research the alteration in some markers of oxidative damage and antioxidant defence system. For this investigation mice were treated with 700 mg/kg D-GalN and 15 I¼g/kg TNF-I± one hour after administration with 8 mg/kg Z-FA.FMK. Treatment with Z-FA.FMK before TNF-I±/D-GalN injection resulted in decreased lipid peroxidation levels, while catalase, superoxide dismutase, glutathione peroxidase, paraoxonase 1 activities and glutathione levels were increased in the brain tissue of mice. These results showed that cathepsin B inhibition by Z-FA. FMK could be a potential therapeutic utility in neuroinflammatory diseases because of its ability to block TNF-I±/D-GalN-induced oxidative damage by increasing antioxidant defence in the brain of mice. Keywords: Benzyloxycarbonyl-Phenyl-Alanine-fluoromethylketone (Z-FA.FMK), Cathepsin B, Dgalactosamine, Oxidative damage, Tumour necrosis factor-I± (TNF-I±).","PeriodicalId":14521,"journal":{"name":"IUFS Journal of Biology","volume":"72 1","pages":"85"},"PeriodicalIF":0.0,"publicationDate":"2012-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"76503261","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Comparison of real-time PCR and conventional cultural methods to detect Escherichia coli O157:H7 in spices in Turkey 实时PCR与传统培养法检测土耳其香料中大肠杆菌O157:H7的比较

IUFS Journal of Biology Pub Date : 2012-01-01 DOI: 10.18478/iufsjb.99956

Asli Ozkizilcik, M. Ateş, B. Çerçi

{"title":"Comparison of real-time PCR and conventional cultural methods to detect Escherichia coli O157:H7 in spices in Turkey","authors":"Asli Ozkizilcik, M. Ateş, B. Çerçi","doi":"10.18478/iufsjb.99956","DOIUrl":"https://doi.org/10.18478/iufsjb.99956","url":null,"abstract":"In this study, 66 different spice samples were analyzed in order to compare Real-Time PCR and conventional cultural methods for the detection of E. coli O157:H7 in spices. Thus, 66 different spice samples (raw materials) were collected from various places in Izmir, Turkey. Conventional cultural methods included pre-enrichment step, selection step and confirmation step. The molecular method included two steps: pre-enrichment and Real-Time PCR step. Beside these methods, total aerobic mesophilic bacteria, coliform, fecal coliform in samples, were also counted and the presence of Salmonella sp. was investigated in order to determine total microbial load in samples. The results showed that aerobic mesophilic bacteria counts were 5.1x103 -2.0x108 CFU/g, coliform counts were 8.0 x 102 CFU/g for the average values of all samples. Salmonella sp. was not found in any of the samples.In four samples E. coli O157:H7 positive by Real-Time PCR were found while all of the samples were negative by cultural methods. We observed that the results of Real-Time PCR were more reliable than conventional methods. Furthermore, the results were obtained in only 20 hours by Real-Time PCR method whereas conventional cultural method was completed in 4 days. Keywords: Cultural method, E. coli O157:H7, real-time PCR, spice","PeriodicalId":14521,"journal":{"name":"IUFS Journal of Biology","volume":"93 1","pages":"113"},"PeriodicalIF":0.0,"publicationDate":"2012-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"75652189","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Biofilm formation on galvanized steel by SRB isolate obtained from cooling tower water 从冷却塔水中提取的SRB分离物在镀锌钢板上形成生物膜

IUFS Journal of Biology Pub Date : 2011-10-27 DOI: 10.18478/IUFSJB.45154

Derya Ozuolmez, A. Çotuk

引用次数: 3

Presentation of flora, life forms and chorology of plant species in the Jahrom area (Fars Province, Iran) 伊朗法尔斯省Jahrom地区植物区系、生命形式和物种的时序介绍

IUFS Journal of Biology Pub Date : 2011-10-27 DOI: 10.18478/IUFSJB.54441

G. Ghanbarian, E. Jafari, A. Hatami

{"title":"Presentation of flora, life forms and chorology of plant species in the Jahrom area (Fars Province, Iran)","authors":"G. Ghanbarian, E. Jafari, A. Hatami","doi":"10.18478/IUFSJB.54441","DOIUrl":"https://doi.org/10.18478/IUFSJB.54441","url":null,"abstract":"In the present study, species were classified according to their growth habit and Raunkiaer's life- forms system were used to characterize the flora of Jahrom. Jahrom in the south east of the Fars Province is an area with about 1,627,470 hectares , maximum altitude at 3167 m and minimum altitude at 300 m, located between 52° 30I„ to 54° 00I„ east latitudes and 28 ° 00I„ to 29° 00I„north longitudes. We studied the area from a floristic and ecological point of view. The results showed that the flora of this region includes 346 species belonging to 234 genera and 67 families. Papilionaceae with 52 species, Asteraceae with 47 species and Poaceae with 36 species are the main families of this area. Plant life forms were studied by Raunkiaer's method. It showed that therophytes with (30.1%) of species, hemicryptophyte with (30.1%) of species, phanerophytes with (17.6%) of species and chamaephyte with (13%) of species, are the dominant life forms of the area. The chorological studies showed that (81.8%) of species belong to Irano-Turanian zone, (9.2%) of species belong to Sahara-Sindian zone and (9%) of common species belong to Irano-Turanian, Sahara-Sindian zones.","PeriodicalId":14521,"journal":{"name":"IUFS Journal of Biology","volume":"130 1","pages":"1-11"},"PeriodicalIF":0.0,"publicationDate":"2011-10-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"81721427","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 5