Italian Journal of Food Safety最新文献

{"title":"Probiotic-fortified <i>Solanum lycopersicum</i> (tomato) juice: free or encapsulated <i>Lactobacillus plantarum</i> and <i>Lactobacillus</i> <i>delbrueckii</i>.","authors":"Reyhane Zakian, Marjan Nouri","doi":"10.4081/ijfs.2025.13932","DOIUrl":"10.4081/ijfs.2025.13932","url":null,"abstract":"<p><p>Recent consumer perception of a nutritious diet improves demand for functional and safety products such as probiotics. The present research aims to investigate enriching Solanum lycopersicum (tomato) juice, including free and encapsulated probiotic bacteria. Initially, physicochemical attributes of encapsulations were evaluated. Then tomato juice samples, including a control without bacteria, free or encapsulated Lactobacillus plantarum (L. plantarum, T1 and T2), Lactobacillus delbrueckii (L. delbrueckii, T3 and T4), and a mixture (T5 and T6), were prepared over 28 days of shelf life. Several assays were performed, such as pH, lycopene, turbidity, stability, antioxidant, probiotic viability, sensory, and structure. Physicochemical functions of encapsulation illustrated that the results were in the suitable range. The pH of all treatments declined, and free L. plantarum demonstrated a greater effect on reduction. The control and encapsulated L. plantarum samples exhibited the lowest lycopene, ranging from 0.64 to 0.35 μL/mL, while the highest ranged from 0.64 to 0.50 μL/mL during the shelf life. Encapsulated dual bacteria indicated higher turbidity, stability, and antioxidant features compared to the control throughout shelf life. The control maintained greater transparency than others, and microbial analysis indicated that probiotic populations were elevated until the 14th day and then reduced. The encapsulated dual-bacteria illustrated the maximum viability and sensory, while the control had the minimum ratings. Morphological analysis confirmed a homogeneous structure for encapsulated bacteria. Overall results depicted that treatments containing encapsulated bacteria are considered the preferred option to promote nutritious juice.</p>","PeriodicalId":14508,"journal":{"name":"Italian Journal of Food Safety","volume":" ","pages":""},"PeriodicalIF":1.7,"publicationDate":"2025-11-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12777941/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145337003","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Dietary exposure assessment to nickel through the consumption of poultry, beef, and pork meat for different age groups in the Italian population.","authors":"Alessio Manfredi, Maria Olga Varrà, Emanuela Zanardi, Maria Vitellino, Mariantonietta Peloso, Patrizio Lorusso, Sergio Ghidini, Elisabetta Bonerba, Damiano Accurso","doi":"10.4081/ijfs.2025.13840","DOIUrl":"10.4081/ijfs.2025.13840","url":null,"abstract":"<p><p>Dietary risk assessment for toxic elements focuses on those listed by Commission Regulation (EU) 2023/915. However, new toxicological evidence suggests expanding research to other elements, including nickel. Classified as carcinogenic by the International Agency for Research on Cancer, nickel exposure mainly occurs through food and water. In individuals with hypersensitivity, oral exposure to this element may trigger symptoms ranging from dermatitis to systemic nickel allergy syndrome. Based on this evidence, the European Food Safety Authority set a tolerable daily intake (TDI) of 13 μg/kg bw/day, recommending further data collection to establish maximum levels in food. In this study, nickel occurrence was evaluated in 809 muscle meat samples (poultry, beef, and pork). Statistical analysis was conducted to identify differences in mean concentrations among the different meat types. Moreover, contamination levels of nickel were used to assess the dietary exposure of different age groups of Italian consumers through meat consumption, providing a comprehensive risk characterization. Toddlers were the most exposed age group, while the elderly were the least exposed. Across all age groups, exposure levels followed the pattern: pork > poultry > beef. Generally, meat consumption contributed less than 1% of the nickel TDI for all the age groups. In particular, the highest contribution to the TDI, equal to 0.86%, was associated with the consumption of pork by toddlers. Therefore, data from this study suggest that nickel contamination in poultry, beef, and pork has a minimal impact on human exposure, posing a negligible risk to public health.</p>","PeriodicalId":14508,"journal":{"name":"Italian Journal of Food Safety","volume":" ","pages":""},"PeriodicalIF":1.5,"publicationDate":"2025-11-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12679953/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144873157","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The relationship between the occurrence of subclinical mastitis and milk quality in medium-sized Holstein cow farms in Albania.","authors":"Renis Maçi, Roland Meçaj, Majlind Sulçe, Florian Plaku, Artan Xhafa, Enkelejda Sallaku, Xhelil Koleci, Myqerem Tafaj","doi":"10.4081/ijfs.2025.13748","DOIUrl":"10.4081/ijfs.2025.13748","url":null,"abstract":"<p><p>Subclinical mastitis (SCM) reduces both milk production and milk quality. In this study, the prevalence of SCM, as determined by the California Mastitis Test (CMT), was analyzed in relation to lactation year, milk density, lactose content, electrical conductivity, and fat content at both the individual cow level and the farm level. The focus was on the overall prevalence of CMT-positive cases within the farms and the mean values of physicochemical changes in milk for each farm. A total of 711 udder quarters from 178 Holstein cows across nine dairy farms were sampled at three intervals during lactation (i.e., in three lactation periods). The number of cows per farm ranged from 21 to 140, covering various lactation stages. A CMT score of 1+ in any quarter was considered SCM-positive. The significance and correlation of SCM's impact on changes in physicochemical milk parameters were analyzed using a robust compound regression. Prevalence of SCM was found to be between 0.14 and 0.63. High prevalence of SCM showed a significant increase in milk conductivity and a significant decrease in milk lactose, protein, solid non-fat (SNF) content, and density. The prevalence of mild and severe SCM varied across different seasons and lactation stages (p<0.05). Significant correlations (p<0.05) between lactation year, lactose content, electrical conductivity, SNF content, and protein were measured at the farm level using a milk analyzer and compared with SCM (R2=0.28; R2=0.41; R2=0.26; R2=0.36; R2=0.39). These findings suggest that physicochemical milk parameters, which are routinely measured in Albanian dairies, can serve as an effective early-warning indicator for dairy farms to detect potential cases of SCM.</p>","PeriodicalId":14508,"journal":{"name":"Italian Journal of Food Safety","volume":" ","pages":""},"PeriodicalIF":1.7,"publicationDate":"2025-11-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12679963/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144591255","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Food safety and public health implications by determining the antibiotic residues in animal-derived food commercially available in southern Punjab, Pakistan.","authors":"Fakhra Naznane, Uzma Maqbool, Muhammad Ismail Chughtai, Imran Khaliq, Aatika Aatika, Sana Mustafa, Zikria Saleem, Zainab Tufail, Tawaf Ali Shah, Mirjalol Ismoilov Ruziboy Ugli, Bekzod Madaminov, Muhammad Usman Qamar","doi":"10.4081/ijfs.2025.12766","DOIUrl":"10.4081/ijfs.2025.12766","url":null,"abstract":"<p><p>This comprehensive study aimed to estimate the prevalence of antimicrobial residues in beef, mutton, chicken, fish, and milk. Twenty fresh samples of chicken, mutton, beef, fish, and bovine milk were collected aseptically from retail shops in Southern Punjab, Pakistan. The enzyme-linked immunosorbent assay (ELISA) technique was used to screen the samples for various antimicrobial residues. The ELISA screening of fish samples revealed residue contamination with tetracycline, as well as the metabolites 3-amino-2-oxazolidinone (AOZ) and 5-methyl-morpholino-3-amino-2-oxazolidinone (AMOZ), with concentration ranges of 2.00-108 parts per billion (ppb), 0.09-0.47 ppb, and 0.01-1.08 ppb, respectively. Chicken samples showed residues of tetracycline, quinolone, AOZ, and AMOZ, with detected residual concentrations ranging from 5.60 to 126 ppb for tetracycline, 18 to 230 ppb for quinolone, 0.20 to 1.96 ppb for AOZ, and 0.01 to 1.02 ppb for AMOZ. In livestock, milk samples exhibited 100% contamination for penicillin and 10% for chloramphenicol residues, with concentrations of 0.2-4.1 ppb and 0.01-0.19 ppb, respectively. Beef samples tested positive for residues of penicillin (20%), tetracycline (40%), and chloramphenicol (45%), with residual ranges of 1.36-88 ppb for penicillin, 3.7-148 ppb for tetracycline, and 0.04-0.37 ppb for chloramphenicol. Mutton samples were contaminated with 10% tetracycline and 25% quinolones, showing residual ranges of 1.28-108 ppb for tetracycline and 34-520 ppb for quinolones. Out of 100 samples, 23 showed residues exceeding the maximum residue limit. Specifically, 30.4% of chicken samples, 17.3% of mutton samples, 30.4% of beef samples, 17.3% of milk samples, and 4.3% of fish samples were affected. This study conclusively demonstrated the presence of various antibiotic residues in food-producing animal samples.</p>","PeriodicalId":14508,"journal":{"name":"Italian Journal of Food Safety","volume":" ","pages":""},"PeriodicalIF":1.7,"publicationDate":"2025-11-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12715669/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145040129","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Development and validation of a rapid test kit for iodine detection in salt.","authors":"Aurang Zeb, Tauqeer Ahmad, Muhammad Yasin, Zahid Mehmood, Aurang Zeb Khan","doi":"10.4081/ijfs.2025.13884","DOIUrl":"10.4081/ijfs.2025.13884","url":null,"abstract":"<p><p>Iodine deficiency disorders are widespread among vulnerable populations in Pakistan. To combat this menace, the Universal Salt Iodization program needs regular monitoring throughout the salt supply chain. Traditional titration methods are limited by accessibility, cost, and time, making rapid test tools a more practical alternative for household surveys. In this context, the current study was designed to develop a rapid test kit for on-the-spot analysis of salt samples and validate it against standard reference methods by applying single- and multiple-observer study designs. Results indicated that the regression plot analysis of the developed kit produced an excellent average R2 value of 0.9949. Percent yield/recovery of the titration method and the rapid test kit was achieved at 97.66% and 88.7-102.4%, along with a maximum value of coefficient of variance of 2.84 and 3.45, respectively. Moreover, the limit of detection/sensitivity was higher for multiple-observer designs (97.08%) as compared to the single-observer design (82.09%). The limit of quantification/specificity declined sharply for the multiple-observer design (40.4%) against the single-observer design (69.7%). This study revealed that despite yielding low specificity for multiple observers and occasional overestimations, the developed kit demonstrated strong potential as a sustainable and efficient alternative tool for iodine testing, thereby supporting and enhancing the overall impact of the salt iodization program in the country.</p>","PeriodicalId":14508,"journal":{"name":"Italian Journal of Food Safety","volume":" ","pages":""},"PeriodicalIF":1.7,"publicationDate":"2025-11-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12679958/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144873156","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A retail market survey on fish fraud from Southern Italy using DNA barcoding.","authors":"Laura Del Sambro, Ashraf Ali, Giovanni Normanno, Loredana Capozzi, Stefano Castellana, Pietro Di Taranto, Fiorenza Petruzzi, Donatella Belluscio, Antonio Parisi, Angelica Bianco","doi":"10.4081/ijfs.2025.13376","DOIUrl":"10.4081/ijfs.2025.13376","url":null,"abstract":"<p><p>Consumption of seafood, which includes both wild and aquaculture products, has increased several-fold during the last 50 years. Species substitution, in which low-value fish are replaced with high-value fish, is one of the prominent phenomena happening in the international seafood trade and the leading cause of fraud in the fishery sector, leading to both economic and health concerns. In this study, DNA barcoding was employed to identify 78 fishery product samples collected from markets and supermarkets located in the Apulia region (Southern Italy) at the genus or species level. Non-compliance between the species detected and the species declared in the label was detected in 5 (6.41%) samples. This study highlights the need for further investigations regarding the traceability and assessment of food product authentication. Indeed, accurate taxonomic assignment and a robust traceability system are essential tools for tackling food adulteration problems, providing transparency, and protecting food safety.</p>","PeriodicalId":14508,"journal":{"name":"Italian Journal of Food Safety","volume":" ","pages":""},"PeriodicalIF":1.7,"publicationDate":"2025-11-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12715670/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145033376","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Impact of volcanic eruptions on heavy metal contamination in the food chain.","authors":"Samuele Pulze, Noemi Presti, Aniello Anastasio","doi":"10.4081/ijfs.2025.13517","DOIUrl":"10.4081/ijfs.2025.13517","url":null,"abstract":"<p><p>This work aims to assess the risk of heavy metal contamination of volcanic origin in the agri-food chain, describing the potential danger to human health. Volcanic phenomena lead to the environmental presence of contaminants, including heavy metals, which influence our ecosystems, food, and health. Opinions have been expressed regarding the presence of heavy metals in our diet and the potential risk of food contamination following volcanic eruptions. Regulation (EU) 915/2023 does not reference the sanctions that would be applied if food products with analytical values exceeding the established tolerance limits for heavy metals were marketed. Legislative Decree No. 18 of February 23, 2023, while providing a sanctions framework, is not specific to the exceedance of maximum limits for heavy metals in water intended for human consumption; it is plausible to apply the Penal Code (Articles 444 and 452) and Law No. 283 of April 30, 1962. The effect of volcanic eruptions seems to play a residual role in heavy metal contamination in food supply chains, considering that their presence is quantified in trace amounts. Given that a limited number of active volcanoes have been monitored for heavy metal emissions and that data regarding the contribution of volcanic activity is lacking, there are still too many missing pieces to conduct a realistic risk assessment of the impact volcanic activity has on the food chain. In conclusion, the 2022 Environmental Data Yearbook from the Higher Institute for Environmental Protection and Research indicates that Italy has achieved the goals set internationally by the Aarhus Convention.</p>","PeriodicalId":14508,"journal":{"name":"Italian Journal of Food Safety","volume":" ","pages":""},"PeriodicalIF":1.7,"publicationDate":"2025-11-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12679960/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144873160","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"MeatCulture: microbiological, hygienic and qualitative profile of bovine fifth quarter viscera.","authors":"Giuliana Siddi, Francesca Piras, Maria Pina Meloni, Mattia Migoni, Mario Cuccu, Roberta Lai, Fabrizio Simbula, Daniela Cabras, Marco Manca, Enrico Pietro Luigi De Santis, Christian Scarano","doi":"10.4081/ijfs.2025.13431","DOIUrl":"10.4081/ijfs.2025.13431","url":null,"abstract":"<p><p>The MeatCulture project aimed to improve the quality and safety of bovine fifth quarter viscera (tripe, intestine, lung, and trachea), which are traditional dish ingredients in many communities. Stabilization studies were conducted on tripe and intestine samples, and preservability studies were conducted on lungs and trachea. Tripe samples (24 across two batches) underwent washing and bleaching treatments with different temperature-time combinations, then they were vacuum-packed, stored at 2±2°C, and analyzed at various timepoints. The pH was determined, and the Enterobacteriaceae count and the presence of Salmonella, Listeria monocytogenes, and Yersinia enterocolitica were investigated. Results indicated that applying a washing step at a temperature range between 55°C and 65°C for 2 minutes, followed by bleaching at a temperature range between 58°C and 70°C for 2 minutes, preserved stable pH (T10: 7.24±0.06) and controlled Enterobacteriaceae counts, which decreased at T7 (2.78±0.48 CFU/g) before slightly increasing at T10 (3.24±1.55). Pathogens were never detected. Part of the small intestine (jejunum) and colon samples were vacuum-packed (30 samples overall), subjected to four types of heat treatments, stored at 2±2°C, and analyzed at various timepoints. pH and microbial profile were evaluated as previously described. The treatment with water at 70°C for 15' showed the best results with Enterobacteriaceae counts below the detection limits in most samples at T10. The lungs (153 samples) and trachea (81 samples) samples were packaged with CO2 and N (ratios of 50/50 and 70/30) modified atmosphere packaging (MAP). The samples were stored at two different temperatures (2±2°C and 8±2°C) and analyzed at various timepoints. On each sample, pH, aerobic colony count, Enterobacteriaceae count, mesophilic lactic acid bacteria count, and Pseudomonas spp. count were evaluated. The microbial profile of the samples stored at 2±2°C and with the 50/50 MAP showed lower average values.</p>","PeriodicalId":14508,"journal":{"name":"Italian Journal of Food Safety","volume":" ","pages":""},"PeriodicalIF":1.7,"publicationDate":"2025-11-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12679954/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145086210","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"<i>Colchicum autumnale</i> poisoning in a dairy farm in Switzerland: a glimpse at the milk safety aspect.","authors":"Karen Barmettler, Adrian Fäh, Hanspeter Naegeli, Sandra N Poetzsch, Simone Weisser, Roger Stephan","doi":"10.4081/ijfs.2025.13828","DOIUrl":"10.4081/ijfs.2025.13828","url":null,"abstract":"<p><p>Colchicine poisoning in livestock presents a significant challenge for dairy farming, particularly in regions where Colchicum autumnale (autumn crocus) is prevalent in pastures. This case report describes an incident on an organic dairy farm in Eastern Switzerland where Brown Swiss cattle showed clinical signs after consuming freshly mown meadow forage containing autumn crocus leaves. The ingestion of this highly toxic plant led to severe effects such as apathy, hypothermia, and reduced milk production, which required immediate veterinary intervention. As colchicine was expected to be secreted in the milk, the milk supplier imposed a delivery stop based on European Commission Regulation (EU) No 37/2010, excluding the use of colchicine as a pharmacologically active substance in food-producing animals. Using liquid chromatography coupled to tandem mass spectrometry, it was shown that a bulk milk sample taken 2 weeks after the poisoning event tested positive, while a further bulk tank milk sample collected 5 weeks after the incident tested negative. Milk deliveries were then resumed. This paper further reviews the current understanding of colchicine as a chemical hazard in milk and presents a toxicological assessment, which is crucial for setting a detection limit for methods aimed at detecting colchicine in milk, given the absence of maximum limits and withdrawal periods following exposure. This study contributes to raising awareness of colchicine as a milk safety concern and to improving the monitoring of toxic plant exposure in livestock management (from feed to food concept).</p>","PeriodicalId":14508,"journal":{"name":"Italian Journal of Food Safety","volume":" ","pages":""},"PeriodicalIF":1.7,"publicationDate":"2025-11-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12679959/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144992383","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Incidence rates of tumors in dogs in southern Italy - focus on the Campania region - 2018/2022.","authors":"Maria Ottaiano, Loredana Baldi, Veronica Del Monaco, Roberta Brunetti, Roberta Pellicanò, Stefania Cavallo, Rosa D'Ambrosio, Federica Gargano, Maria Dimatteo, Marianna D'Amore, Guido Rosato, Marina Pompameo, Barbara Degli Uberti, Esterina De Carlo","doi":"10.4081/ijfs.2025.13402","DOIUrl":"10.4081/ijfs.2025.13402","url":null,"abstract":"<p><p>Neoplastic disease in dogs and humans has many similarities, including similar clinical manifestations, metastatic potential, and genomic instability. However, tumor latency is shorter in dogs, which may serve as a sentinel for the identification of carcinogenic environmental exposures. This study aims to describe the data collected in the Animal Tumor Registry (RTA) of the Campania Region in the period 2018-2022, with particular attention to the incidence rates of malignant tumors in dogs, divided by province. RTA tumor cases received by the Istituto Zooprofilattico Sperimentale del Mezzogiorno were analyzed; out of a total of 885 tumors, 70% were malignant, with a higher frequency in female dogs. The crude and standardized incidence rate of malignant tumors in dogs was calculated on a provincial basis. Caserta showed the highest incidence rate for both malignant tumors [incidence rate: 135.07 per 100,000; 95% confidence interval (CI): 135.01-135.13] and mammary tumors (incidence rate: 52.75 per 100,000; 95% CI: 52.69-52.80). The results highlight the importance of tumor monitoring in dogs as an indicator of potential environmental risk factors and underline the value of the RTA for the collection of data useful for the prevention and study of neoplastic diseases, as well as in humans.</p>","PeriodicalId":14508,"journal":{"name":"Italian Journal of Food Safety","volume":" ","pages":""},"PeriodicalIF":1.7,"publicationDate":"2025-11-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12715672/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144626357","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}