Iolanda Venuti, Marina Ceruso, Giuseppe Palma, Giorgio Smaldone, Tiziana Pepe
{"title":"DNA barcoding and nutritional analysis as a tool for promoting the market of inland fish species.","authors":"Iolanda Venuti, Marina Ceruso, Giuseppe Palma, Giorgio Smaldone, Tiziana Pepe","doi":"10.4081/ijfs.2021.9565","DOIUrl":"https://doi.org/10.4081/ijfs.2021.9565","url":null,"abstract":"<p><p>The increasing world market demand for seafood requires an expansion of product categories available to consumers. Inland fish are usually considered having unmarked taste and are less appreciated by consumers; thus, they have low commercial value. Therefore, the marketing of the lake's fresh and processed fish is limited to the local market and consumers are currently uninformed and mistrustful about these species. In this study, six different fish species were caught in the Fondi lake (Lazio, central Italy): <i>Anguilla anguilla</i>, <i>Tinca tinca</i>, <i>Carassius gibelio</i>, <i>Cyprinus carpio</i>, <i>Micropterus salmoides</i>, <i>Chelon ramada</i>. All the samples were subjected to nutritional and DNA barcoding analysis. Moisture, protein, fat, carbohydrates, ash, and sodium content were measured. As regards the fatty acids profile, the most abundant were MUFAs with the highest value in <i>Anguilla anguilla</i> (45.97%). Oleic acid (C18: 1 n9 cis) was particularly high in <i>Cyprinus carpio</i> (55.46%). The fraction of polyunsaturated fatty acids (PUFA) revealed a higher DHA content (C22: 6 n3) in <i>Anguilla anguilla</i> than the other species (>12 %) while <i>Chelon ramada</i> presented both higher EPA content (C 20: 5 n3) and total fraction of omega 3 PUFAs. Concerning molecular analysis, a 655 bp fragment of cytochrome C oxidase subunit I (<i>COI</i>) gene was successfully used for the identification at the species level using both BOLD and BLAST public databases. The present study gives the basis for improving the knowledge and promoting inland fish' market and traceability along the supply chain.</p>","PeriodicalId":14508,"journal":{"name":"Italian Journal of Food Safety","volume":"10 3","pages":"9565"},"PeriodicalIF":1.3,"publicationDate":"2021-10-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/0d/ef/ijfs-10-3-9565.PMC8513186.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"39588969","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Federico Tomasello, Marta Pollesel, Elisabetta Mondo, Federica Savini, Raffaele Scarpellini, Federica Giacometti, Luna Lorito, Marco Tassinari, Sean Cuomo, Silvia Piva, Andrea Serraino
{"title":"Effectiveness of alkaline electrolyzed water in reducing bacterial load on surfaces intended to come into contact with food.","authors":"Federico Tomasello, Marta Pollesel, Elisabetta Mondo, Federica Savini, Raffaele Scarpellini, Federica Giacometti, Luna Lorito, Marco Tassinari, Sean Cuomo, Silvia Piva, Andrea Serraino","doi":"10.4081/ijfs.2021.9988","DOIUrl":"https://doi.org/10.4081/ijfs.2021.9988","url":null,"abstract":"<p><p>Alkaline electrolyzed water (REW) is known for its cleaning action. The aim of this work was to assess REW effectiveness in reducing microbial load on surfaces intended for contact with food. Stainlesssteel surfaces were experimentally contaminated, bacterial inactivation was tested before and after treatment with REW. Treatment with REW was operated spraying it on the contaminated plates until drying. Tests were conducted for <i>Salmonella</i> spp., <i>Listeria</i> spp., <i>Staphylococcus aureus</i> and <i>Escherichia coli</i>. The treatment revealed different degrees of sanitizing activity of REW on different bacterial species, with higher efficacy on <i>E</i>. <i>coli</i> and <i>Salmonella</i> spp. than <i>S. aureus</i>, <i>Listeria</i> spp.. Statistical analysis revealed a significant microbial load reduction (p<0.01) after treatment with REW, suggesting that it has a good disinfectant activity which, along with its easy and safe use, makes it a good alternative to many other more widely used disinfectants.</p>","PeriodicalId":14508,"journal":{"name":"Italian Journal of Food Safety","volume":"10 3","pages":"9988"},"PeriodicalIF":1.3,"publicationDate":"2021-10-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/39/16/ijfs-10-3-9988.PMC8513190.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"39677718","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Alessio Parco, Giusi Macaluso, Maria Foti, Maria Vitale, Vittorio Fisichella, Marco Tolone, Guido Ruggero Loria
{"title":"Phenotypic and genotypic study on antibiotic resistance and pathogenic factors of <i>Staphylococcus aureus</i> isolates from small ruminant mastitis milk in South of Italy (Sicily).","authors":"Alessio Parco, Giusi Macaluso, Maria Foti, Maria Vitale, Vittorio Fisichella, Marco Tolone, Guido Ruggero Loria","doi":"10.4081/ijfs.2021.9722","DOIUrl":"10.4081/ijfs.2021.9722","url":null,"abstract":"<p><p><i>Staphyloccoccus aureus</i> is the major cause of mastitis in small ruminants in the Mediterranean farms causing severe losses to dairy industry. Antibiotic treatment has been the most common approach to control these infections. Aim of this study was to investigate antimicrobial resistance (AMR), virulence factors and biofilm-related genes of 84 Sicilian strains of <i>S. aureus</i> isolated from sheep and goats milk during two different periods δT<sub>1</sub> (2006-2009) and δT<sub>2</sub> (2013-2015). Kirby Bauer method and Polymerase Chain Reaction (PCR) were utilized to monitor AMR and related genes (<i>mecA, tetK, tetM, ermA, ermC</i>). Moreover, toxin genes (<i>tsst-1, sea-see, seg-sej</i>, and <i>sep</i>) and biofilm genes (<i>bap, ica, sasC</i>) were studied. Twenty-six isolates (30.9%) showed multidrug resistance. The two groups showed similar results with exception for higher values of resistance for tilmicosin and lower for sulfamethoxazole and vancomycin of the second group. <i>MecA</i> gene was detected in one isolate. Tetracycline resistance was higher than 20%, with an increase in δT<sub>2</sub> group. Toxin genes were found in 5 isolates (5.9%), belonging of δT<sub>2</sub> group, while 57 of isolates (67.8%) showed biofilm related genes. The high presence of multi-resistant isolates suggests the need of more responsible use of antibiotic therapy for the control of these infections.</p>","PeriodicalId":14508,"journal":{"name":"Italian Journal of Food Safety","volume":"10 3","pages":"9722"},"PeriodicalIF":1.3,"publicationDate":"2021-10-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/a4/00/ijfs-10-3-9722.PMC8513188.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"39588970","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Ineta Simonavičienė, Gintarė Zakarienė, Aušra Lozoraitytė, Gintarė Zaborskienė, Gediminas Gerulis, Artūras Stimbirys
{"title":"Identification and serotyping of <i>Listeria monocytogenes</i>, isolated from various salmon products, sold in retail market in Lithuania.","authors":"Ineta Simonavičienė, Gintarė Zakarienė, Aušra Lozoraitytė, Gintarė Zaborskienė, Gediminas Gerulis, Artūras Stimbirys","doi":"10.4081/ijfs.2021.9341","DOIUrl":"10.4081/ijfs.2021.9341","url":null,"abstract":"<p><p>Cold smoked salmon products (belly flaps, pieces, fillet, and loin) obtained from the retail market in Lithuania were tested for the presence of <i>L. monocytogenes</i>. It was found that contamination of the cold smoked fish products with <i>Listeria</i> spp. depends on the type of the product. Contamination with listeria in salmon belly flaps was 7.5 times higher than in the loin (P<0.05), 1.8 times higher than in the pieces (P<0.05) and 30 times higher than in the fillet (P<0.05). Microbiological analysis showed that 32.5% (P<0.05) of the fish product samples were infected with <i>L. monocytogenes</i>, while multiplex PCR confirmed 31.25% positive samples (P<0.01)<i>.</i> According to the study results, <i>L. monocytogenes</i> strains were divided into two serotypes: 4b (94.6%) and 1/2a (5.4%). High contamination of the products with <i>Listeria</i> spp. showed that cold smoked salmon products, sold in local market, can be a reason of human listeriosis in Lithuania.</p>","PeriodicalId":14508,"journal":{"name":"Italian Journal of Food Safety","volume":"10 3","pages":"9341"},"PeriodicalIF":1.8,"publicationDate":"2021-09-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/0e/6a/ijfs-10-3-9341.PMC8491009.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"39562542","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Natalia Montero, Marco Atzori, Barbara Marras, Alexandre Bettoschi, Pierpaolo Nurchis, Valentina Coroneo, Clara Sanna, Marco Schintu
{"title":"Trace metal levels in the edible tissues of sea cucumbers (<i>Holothuria tubulosa and Holothuria polii</i>) from Sardinia (Western Mediterranean).","authors":"Natalia Montero, Marco Atzori, Barbara Marras, Alexandre Bettoschi, Pierpaolo Nurchis, Valentina Coroneo, Clara Sanna, Marco Schintu","doi":"10.4081/ijfs.2021.9576","DOIUrl":"https://doi.org/10.4081/ijfs.2021.9576","url":null,"abstract":"<p><p>Sea cucumbers represent an important part of the diet in Asian and Pacific regions and are also used in traditional medicine. These habits have led to the overexploitation of local sea cucumber populations in these areas, driving the pursuit of new stock regions, such as Mediterranean areas. In Italy, contrarily to that observed for other Mediterranean countries, the exploitation of sea cucumber stocks is not extensive, which opens a new market opportunity. Thus, from a food safety perspective, this work aims at reporting the first assessment of trace metal concentrations (As, Cd, Cr, Cu, Hg, Ni, Pb) in the edible tissues of <i>Holothuria polii</i> and <i>Holoturia tubulosa</i> collected in Sardinia, the second-largest island in the Mediterranean Sea. Metal concentrations found in <i>H. polii</i> were generally higher than in <i>H. tubulosa</i>. However, in both species, they were lower than those reported for other areas of the Western Mediterranean. Cd, Hg, and Pb were below the limits established for seafood in Europe. As concentrations were in the range of those measured in other commercial seafood species in the Mediterranean. Thus, these species may be harvested and traded to fulfil the demands of local and international markets.</p>","PeriodicalId":14508,"journal":{"name":"Italian Journal of Food Safety","volume":"10 3","pages":"9576"},"PeriodicalIF":1.3,"publicationDate":"2021-09-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/99/a5/ijfs-10-3-9576.PMC8491012.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"39562544","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Update on animal welfare perception by Italian consumers: A descriptive survey.","authors":"Giulia Rubini, Eleonora Nannoni, Jorgelina Di Pasquale, Giovanna Martelli, Luca Sardi","doi":"10.4081/ijfs.2021.9588","DOIUrl":"https://doi.org/10.4081/ijfs.2021.9588","url":null,"abstract":"<p><p>The aim of this study was to evaluate if and how the perception of animal welfare by Italian consumers and their purchasing behavior of animal-friendly foods have evolved in recent years. The research was carried out through a survey on a representative sample of Italian consumers (n=969). Responses showed that 69% of consumers declared to pay attention to animal welfare at time of purchasing, and they mostly acquired information about animal welfare through the mass media. In accordance with previous surveys, the categories perceived as having the highest welfare level were fish and dairy cows, whereas the lowest welfare level was attributed to broilers and pigs. Compared to previous surveys, Italian consumers seem to have improved their knowledge about the legislation on animal protection and increased their interest toward animal welfare issues. However, they still find it difficult to identify animalfriendly labels, together with a lack of knowledge about non-conventional (<i>e.g.</i> extensive and organic) farming systems.</p>","PeriodicalId":14508,"journal":{"name":"Italian Journal of Food Safety","volume":"10 3","pages":"9588"},"PeriodicalIF":1.3,"publicationDate":"2021-09-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/05/c4/ijfs-10-3-9588.PMC8491011.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"39562545","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Giuliana Siddi, Francesca Piras, Vincenzo Spanu, Mariella Demontis, Maria Pina Meloni, Rita Sanna, Veronica Cibin, Enrico Pietro Luigi De Santis, Christian Scarano
{"title":"Trend of <i>Salmonella enterica</i> occurrence and serotypes in Sardinian pig slaughterhouses.","authors":"Giuliana Siddi, Francesca Piras, Vincenzo Spanu, Mariella Demontis, Maria Pina Meloni, Rita Sanna, Veronica Cibin, Enrico Pietro Luigi De Santis, Christian Scarano","doi":"10.4081/ijfs.2021.9362","DOIUrl":"10.4081/ijfs.2021.9362","url":null,"abstract":"<p><p>The aim of this study was to evaluate <i>Salmonella</i> prevalence and serotypes in four Sardinian pig slaughterhouses. Moreover, a population study was conducted with pulsed field gel electrophoresis (PFGE). The results were compared with previous investigations carried out during years 2008 and 2014. A total of 147 samples were collected, 117 from slaughtered pigs (lymph nodes, colon content and carcass surface) and 30 from the slaughterhouse environment (surfaces in contact and not in contact with meat). <i>Salmonella</i> was isolated from 3.4% pig samples and was not detected from environmental samples. Comparing the results with those of previous investigations, occurrence showed a sharp decrease through the years in both animals (18.8% in 2008, 10% in 2014 and 3.4% in 2020) and environmental samples (34.1% in 2008, 3.7 in 2014, and 0% in 2020). At the same time, prevalence of carriers (pigs positive at lymph nodes and/or colon content level) showed a reduction through the years and was always lower in animals coming from local farms rather than those coming from other European Member States, probably indicating the role of stressful factors as transport in increasing <i>Salmonella</i> susceptibility and shedding. <i>Salmonella</i> serotypes were monophasic Typhimurium, Rissen and Muenchen. Overall, 13 different <i>Salmonella</i> serotypes were identified during the three surveys with the most prevalent being serotypes often isolated from slaughtered pigs and during human salmonellosis cases: <i>S.</i> Derby and <i>S</i>. Typhimurium in 2008, <i>S.</i> Anatum and <i>S.</i> Rissen in 2014, monophasic <i>S.</i> Typhimurium in 2020. Population study with pulsed field gel electrophoresis showed a high similarity between <i>Salmonella</i> strains belonging to the same serotype. The results of the investigations showed a decrease of <i>Salmonella</i> occurrence during twelve years in Sardinia, probably due to the improvement in the application of correct GMPs and GHPs at slaughterhouse and also to a reduction of the rate of carrier pigs at farm level.</p>","PeriodicalId":14508,"journal":{"name":"Italian Journal of Food Safety","volume":"10 2","pages":"9362"},"PeriodicalIF":1.3,"publicationDate":"2021-08-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/82/01/ijfs-10-2-9362.PMC8419715.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"39424416","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Hani Dimassi, Rita Haddad, Rana Awada, Lama Mattar, Hussein F Hassan
{"title":"Food shopping and food hygiene related knowledge and practices during the COVID-19 pandemic: The case of a developing country.","authors":"Hani Dimassi, Rita Haddad, Rana Awada, Lama Mattar, Hussein F Hassan","doi":"10.4081/ijfs.2021.9384","DOIUrl":"https://doi.org/10.4081/ijfs.2021.9384","url":null,"abstract":"<p><p>The aim of this study was to assess the level of food hygiene and food shopping knowledge and practices among residents of Lebanon during the COVID-19 pandemic, and to identify their socio-demographic determinants. A cross-sectional study was conducted through an online questionnaire composed of 13 questions about demographics, and 25 questions related to knowledge and practices in terms of hand maintenance, mask placing, and food shopping. A total of 1337 participants filled the survey. On average, participants scored 73±15 and 67±17% on food hygiene and knowledge and practices, respectively. In terms of best practices by section, they scored 77±22, 51±42, and 65±23% on hand maintenance, mask placing and shopping, respectively. Hand maintenance best practices score was significantly (p<0.05) affected by gender; mask placing best practices score was significantly affected by age and major of study; shopping best practices score was significantly affected by gender; overall best practices score was significantly affected by gender and major of study; food hygiene knowledge score was significantly affected by gender, age, marital status, educational level, and major of study. Food hygiene practices and knowledge scores were significantly (p<0.001) related with a weak correlation coefficient (R=0.114). No study has determined the food hygiene and shopping knowledge and practices during the COVID-19 pandemic in a developing country before.</p>","PeriodicalId":14508,"journal":{"name":"Italian Journal of Food Safety","volume":"10 2","pages":"9384"},"PeriodicalIF":1.3,"publicationDate":"2021-07-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/a2/fb/ijfs-10-2-9384.PMC8404529.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"39396794","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Alberto Olivastri, Barbara Paoletti, Carlotta Lauteri, Luca Pennisi, Domenico Paludi, Anna Rita Festino, Alberto Vergara
{"title":"Parasitic cysts in wild boars hunted in Central Italy: The sanitary controls in the wild game meats chain.","authors":"Alberto Olivastri, Barbara Paoletti, Carlotta Lauteri, Luca Pennisi, Domenico Paludi, Anna Rita Festino, Alberto Vergara","doi":"10.4081/ijfs.2021.9383","DOIUrl":"10.4081/ijfs.2021.9383","url":null,"abstract":"<p><p>Seven hundred sixty-five (765) adult wild boars were examined during the 2016/2017 hunting season for the research of parasites. <i>Post mortem</i> inspection was carried out at the slaughterhouse by the Official Veterinarian on the plucks (heart, tongue, lungs, diaphragm, and liver) of the killed animals presented by hunters. Of these, 0.8% (6/765) were positive for <i>Echinococcus granulosus sensu lato</i> (s.l.), and 2.6% (20/765) were positive for the metacestode stage of <i>Taenia hydatigena</i> (<i>Cysticercus tenuicollis</i>), while 0.5% (4/765) animals showed a mixed infection (<i>Echinococcus granulosus</i> s.l. and <i>Taenia hydatigena</i>). Sixty-three (63) cystic lesions were found. Of these 25,4% (16/63) were caused by <i>Echinococcus granulosus</i> s.l. and 74,6% (47/63) were caused by <i>Cysticercus tenuicollis</i>. The more involved organs were liver and lungs, in a less extension omentum and diaphragm. Parasitological analyses showed an overall prevalence of 3.9% for metacestodes in the hunted animals examined (Paoletti <i>et al</i>., 2018). Hydatids were molecularly characterized as <i>E. granulosus sensu stricto</i>. <i>Trichinella spp</i>. examination results showed no evidences of parasitic cysts. The products of hunting used for own consumption and direct sale to the final consumer or retailer, according to Regulation (EC) No 853/2004, lack of overall control by the Competent Authority. This is a critical point in the food chain of the game meat. The data obtained show the importance of the <i>post mortem</i> inspection and the central role of the Competent Authority to ensure not only the food safety of game meat but also to collect data for extensive epidemiological investigations on live-stocks wildlife settings having a direct impact on public health.</p>","PeriodicalId":14508,"journal":{"name":"Italian Journal of Food Safety","volume":"10 2","pages":"9383"},"PeriodicalIF":1.3,"publicationDate":"2021-07-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/24/1c/ijfs-10-2-9383.PMC8404528.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"39396792","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Luigi Crobu, Alessandro Graziano Mudadu, Rita Melillo, Giovanni Luigi Pais, Domenico Meloni
{"title":"Qualitative determination of histamine in canned yellowfin tuna (<i>Thunnus albacares</i>) marketed in Sardinia (Italy) by rapid screening methods.","authors":"Luigi Crobu, Alessandro Graziano Mudadu, Rita Melillo, Giovanni Luigi Pais, Domenico Meloni","doi":"10.4081/ijfs.2021.9379","DOIUrl":"https://doi.org/10.4081/ijfs.2021.9379","url":null,"abstract":"<p><p>Histamine is produced by the bacterial decarboxylation of histidine, an ammino acid present in large amount especially in scombroid fish such as tuna. Fish containing high levels of histamine have been associated with many instances of \"scombroid poisoning\". Since histamine is heat resistant, its presence has been used as an indicator of the good manufacturing practice and of the preservation state of canned tuna. In this study we have applied a rapid screening method to determine the presence of histamine in canned tuna marketed in Sardinia (Italy). A total of 165 samples of canned tuna were screened for the qualitative determination of histamine by HistaSure™ Fish Rapid Test. The results were consistently in agreement with the food safety criteria (<100 mg/kg of histamine) laid down in EC Regulation 2073/2005 (as amended). The HistaSure™ kit was confirmed as a rapid screening method for the presence of histamine in canned tuna.</p>","PeriodicalId":14508,"journal":{"name":"Italian Journal of Food Safety","volume":"10 2","pages":"9379"},"PeriodicalIF":1.3,"publicationDate":"2021-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/b0/1a/ijfs-10-2-9379.PMC8273629.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"39261558","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}