{"title":"Global perceptions and acceptance of irradiated food: a comparative systematic review.","authors":"Jaber Maataoui, Malek Abduljaber, Mohamed Khaddor","doi":"10.4081/ijfs.2025.12885","DOIUrl":"10.4081/ijfs.2025.12885","url":null,"abstract":"<p><p>Irradiated food acceptance around the world exhibits systematic variabilities across many facets related to citizens' perceived approval ratings of the product. The present research summarizes the empirical evidence on the extent to which people around the world accept irradiated food. A systematic review methodology structured the design and implementation of the present analysis. The authors performed comprehensive searches for studies featuring acceptance rates in two broad databases: Web of Science and Scopus. A total of 27 peer-reviewed articles in the English language covering 24,474 people in 15 different countries provided the survey-based data for the investigation. Results indicated that acceptance of irradiated food increased by a large margin in the past 35 years (33% in 1992 to 67% in 2024). Citizens' refusal to purchase irradiated food decreased from 19% to 16% globally in the same period. One of the emerging trends in the results was the rate of irradiated food familiarity, which remained relatively unchanged at 50% in the same time frame. Globally, acceptance of irradiated food is high and rising. Information campaigns and education about the benefits of irradiated food have led to increases in awareness and familiarity. Despite improving perceptions globally, stark variability still exists in acceptance rates, with developing countries having lower acceptance compared to highly industrialized countries. This is the first comparative analysis of different populations' perceptions of irradiated food worldwide. The paper provides new estimates on global acceptance of irradiated food and highlights variability among countries, offering valuable insights for policymakers interested in investing in it.</p>","PeriodicalId":14508,"journal":{"name":"Italian Journal of Food Safety","volume":" ","pages":""},"PeriodicalIF":1.8,"publicationDate":"2025-05-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143483132","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Valeria Rondinone, Lucia Palazzo, Angelica Bianco, Giuseppe La Rosa, Viviana Manzulli, Domenico Galante, Laura Del Sambro, Mariateresa Toce, Antonella Cristina Romano, Lorenzo Pace
{"title":"Identification of <i>Actinobacillus seminis</i> as the cause of abortion in sheep by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry and whole genome sequencing.","authors":"Valeria Rondinone, Lucia Palazzo, Angelica Bianco, Giuseppe La Rosa, Viviana Manzulli, Domenico Galante, Laura Del Sambro, Mariateresa Toce, Antonella Cristina Romano, Lorenzo Pace","doi":"10.4081/ijfs.2025.13384","DOIUrl":"10.4081/ijfs.2025.13384","url":null,"abstract":"<p><p>Actinobacillus seminis is a gram-negative bacterium that affects reproductive organs, causing epididymitis, low fertility, and occasional abortions in sheep and goats. The virulence factors and the pathogenicity mechanisms of A. seminis have not been clearly elucidated yet. The aim of this study is to report a laboratory investigation performed on a sheep farm in the Basilicata region (southern Italy) following cases of abortion. Four samples of aborted fetuses taken from two sheep were analyzed to investigate the main pathogens causing abortions by bacterial isolation. In addition, serum samples belonging to the sheep have also been analyzed. Analysis by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) of the isolated colonies identified protein spectra referable to A. seminis. This result was also confirmed through the use of genome sequencing. Therefore, these data could explain cases of abortion that occurred on the farm and would confirm that MALDI-TOF MS technology is a rapid, accurate, and inexpensive method for identifying bacteria.</p>","PeriodicalId":14508,"journal":{"name":"Italian Journal of Food Safety","volume":" ","pages":""},"PeriodicalIF":1.8,"publicationDate":"2025-05-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143515586","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Evaluation of hygienic food handling practices and associated factors among food handlers in the Amhara region, Ethiopia: a systematic review and meta-analysis.","authors":"Lamenew Fenta, Kebadu Tadesse","doi":"10.4081/ijfs.2025.12836","DOIUrl":"10.4081/ijfs.2025.12836","url":null,"abstract":"<p><p>Foodborne illnesses as a result of poor food handling practices pose a significant threat to public health. The main objective of this systematic review and meta-analysis was to pool the level of hygienic food handling practices among food handlers working in public food establishments in the Amhara region, Ethiopia. An inclusive search of databases was undertaken using PubMed/MEDLINE, SCOPUS, Web of Science, and Google Scholar from the 1st of January 2014 to the 30th of December 2023. Data was collected, entered into Excel, and finally exported to Stata V.17 for analysis. Eyeball testing using forest plots, Cochrane Q test statistics and I2 had been used to identify and measure heterogeneity. The pooled prevalence of hygienic food handling practices was estimated using a random effects model. The pooled prevalence of hygienic food handling practices of food handlers in the Amhara region was 48% [95% confidence interval (CI): (43%, 53%)] with significant heterogeneity (I2=94.39%, p<0.05). Food handlers who have more than 2 years of food preparation experience [odds ratio (OR) = 3.51; 95% CI (2.64, 4.38)] and who got food preparation training [OR=2.14; 95% CI (1.06, 3.22)] were significantly associated with good food handling practice in the Amhara region. The pooled prevalence of hygienic food handling practices was low in the Amhara region, Ethiopia. This review indicated that a high level of experience and training in food preparation were the determinants of good food handling practices in the region. This finding can be used by policymakers, non-governmental organizations, and stakeholders for intervention and regulatory measures.</p>","PeriodicalId":14508,"journal":{"name":"Italian Journal of Food Safety","volume":" ","pages":""},"PeriodicalIF":1.8,"publicationDate":"2025-05-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143255562","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Isabela De Sainz, Mauricio Redondo-Solano, Godofredo Solano, Lautaro Ramírez
{"title":"Optimization of process conditions and kinetic microbial growth for milk fermentation using domestic kefir grains from Costa Rica.","authors":"Isabela De Sainz, Mauricio Redondo-Solano, Godofredo Solano, Lautaro Ramírez","doi":"10.4081/ijfs.2025.12477","DOIUrl":"https://doi.org/10.4081/ijfs.2025.12477","url":null,"abstract":"<p><p>Kefir, a fermented milk product, differs from yogurt due to its unique microbial composition, offering a broad spectrum of health benefits. Given its global popularity and high cost, there is a significant trend towards domestic kefir production. This study explores the optimization of kefir fermentation using Costa Rican domestic kefir grains, assessing the effects of temperature, agitation, and initial starter culture concentration. A central composite rotatable design and response surface statistical approach were employed to evaluate these parameters. Microbial growth data were fitted into a quadratic model, revealing significant interactions, particularly with temperature affecting both lactic acid bacteria (LAB) and yeast populations. Optimized fermentation conditions were established at 25°C, 0 rpm, and 5 g/L initial biomass, under which final microbial populations reached 9.45±0.13 log(cfu)/mL for yeast and 9.23±0.06 log(cfu)/mL for LAB. The specific growth velocity for kefir biomass was 0.029 1/h and the total acid production rate was 0.060 g/(L h). Notably, the acetic acid production was significantly less than lactic acid, indicating a dominance of LAB over acetic acid bacteria, which is crucial for the desired flavor and health benefits of kefir. Additionally, microbial enumeration on glucose-yeast extract calcium carbonate agar and Rogosa agar showed distinct colony formations, highlighting the complex microbial interactions within kefir. This comprehensive dataset suggests that the performance of non-commercial starter cultures can be significantly improved under controlled conditions, providing a basis for developing guidelines for domestic kefir production. This study not only optimizes kefir production but also ensures that home-prepared kefir can meet quality standards, potentially enhancing its nutritional and therapeutic benefits.</p>","PeriodicalId":14508,"journal":{"name":"Italian Journal of Food Safety","volume":" ","pages":""},"PeriodicalIF":1.8,"publicationDate":"2025-05-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144018518","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Physicochemical and organoleptic properties of moringa instant (<i>Moringa oleifera Lam</i>) drink enriched with ginger, turmeric, galangal, and lemongrass.","authors":"Rostiati Dg Rahmatu, Amalia Noviyanty, Fathurrahman Fathurrahman, Syahraeni Kadir, Eko Priyantono, Septian Palma Ariany, Nurpiani Unda","doi":"10.4081/ijfs.2025.13512","DOIUrl":"https://doi.org/10.4081/ijfs.2025.13512","url":null,"abstract":"<p><p>This research aimed to evaluate the impact of various herbal additions on the quality and sensory attributes of moringa (Moringa oleifera Lam)-based instant beverages. The study was conducted in several laboratories at Tadulako University and the Goods Quality Testing and Certification Unit in Palu. Physicochemical properties (water content, vitamin C, antioxidants, solution pH, and dissolution time) were evaluated using a completely randomized design. At the same time, sensory attributes such as color, aroma, and taste were examined using a randomized block design. The experiment tested six different herbal additions: P0 (no herbs), P1 (red ginger), P2 (lemongrass), P3 (galangal), P4 (white turmeric), and P5 (yellow turmeric), with each treatment repeated three times. Results showed that yellow turmeric yielded the best physicochemical outcomes, with a dissolution time of 83 seconds, a water content of 5.27%, a pH of 6.36, a vitamin C content of 12.44%, and an antioxidant activity of 49.25%. In contrast, red ginger emerged as the most preferred by panelists, scoring highest in terms of color, aroma, and taste, with values of 3.50, 3.97, and 3.60, respectively. The findings indicate that herbal additives can enhance the functional properties and sensory appeal of moringa instant drinks. Yellow turmeric effectively optimized physicochemical traits, making the beverage more suitable for health-conscious consumers. Meanwhile, red ginger improved sensory appeal, increasing the likelihood of consumer acceptance. Future research should optimize the concentrations of yellow turmeric and red ginger to balance their effects on physicochemical and sensory qualities, investigate advanced drying techniques to meet Indonesian National Standard 4320:1996 water content standards, and explore the bioavailability and health impacts of these products.</p>","PeriodicalId":14508,"journal":{"name":"Italian Journal of Food Safety","volume":" ","pages":""},"PeriodicalIF":1.8,"publicationDate":"2025-04-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144002025","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Set-up and validation of end-point polymerase chain reaction for the detection of edible insect DNA - <i>Acheta domesticus</i> and <i>Tenebrio molitor</i> - in food products.","authors":"Giulia Magagna, Serena Pederzani, Michela Tilola, Marina Nadia Losio, Virginia Filipello","doi":"10.4081/ijfs.2025.13551","DOIUrl":"https://doi.org/10.4081/ijfs.2025.13551","url":null,"abstract":"<p><p>Insects offer an alternative protein supply for both animal and human consumption. Recently, companies in Europe have begun insect production for use in food and animal feed. Following Regulation (EU) No. 2015/2283, edible insects are classified under the novel food category and can be marketed and consumed within the European Union. As a result, methods of authentication are necessary to verify the compliance of these insect-based products. In this study, two end-point polymerase chain reaction assays for the detection of the 16S rRNA gene of Acheta domesticus and Tenebrio molitor were fine-tuned and validated. The primer specificity was tested on insect samples, other animals, and plants, and the gene was amplified only in target samples. The limit of detection was evaluated using different food matrices contaminated with decreasing concentrations of insect powder and was below 0.05% (w/w).</p>","PeriodicalId":14508,"journal":{"name":"Italian Journal of Food Safety","volume":" ","pages":""},"PeriodicalIF":1.8,"publicationDate":"2025-04-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144010060","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Antibiotic residues and heavy metals in blue crabs (<i>Callinectes sapidus</i>) fished in the Mediterranean Sea: a preliminary study.","authors":"Alessio Manfredi, Patrizio Lorusso, Annamaria Pandiscia, Elisabetta Bonerba, Edmondo Ceci, Giancarlo Bozzo, Domenico Cristofaro, Valentina Terio","doi":"10.4081/ijfs.2025.13287","DOIUrl":"https://doi.org/10.4081/ijfs.2025.13287","url":null,"abstract":"<p><p>In recent decades, the Mediterranean Sea has experienced the invasion of the blue crab (Callinectes sapidus), which threatens the marine ecosystem and economic activities related to fishing and aquaculture because of its aggressive behavior. Control strategies are developing to reduce its population. In Italy, a partial solution to the problem is its promotion as a food product. However, to ensure consumer safety, promoting consumption must be accompanied by a careful risk analysis. This study aims to assess heavy metals and antibiotic residues in the appendage muscle of 18 blue crab samples from various Mediterranean sites, selected by sex and size. Heavy metals were quantified using inductively coupled plasma mass spectrometry, and antibiotics were analyzed with the liquid chromatography/triple-quadrupole tandem mass spectrometry multiresidues/multiclass method. In all samples, lead was never detected, while cadmium and mercury concentrations never exceeded the limit of 0.5 mg/kg set by Regulation (EU) 2023/915. Only one sample tested positive for the occurrence of 12 antibiotic residues. The results showed that the blue crab is a species commonly characterized by the accumulation of heavy metals, according to other studies. Therefore, monitoring the concentration of metals in these species is important for food safety and ecosystem management. Data on 12 antibiotic residues detected in a single crab sample require further investigation through extensive sampling in terms of both number and sites, involving a wider area along the Mediterranean Sea coast, to allow for proper risk characterization.</p>","PeriodicalId":14508,"journal":{"name":"Italian Journal of Food Safety","volume":" ","pages":""},"PeriodicalIF":1.8,"publicationDate":"2025-04-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144003068","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Gianluigi Ferri, Vincenzo Olivieri, Chiara Di Vittori, Alberto Vergara
{"title":"Seasonal prevalence and antimicrobial resistance profiles in <i>Enterococcus</i> spp. identified from mussels farmed along the coasts of the Abruzzo region.","authors":"Gianluigi Ferri, Vincenzo Olivieri, Chiara Di Vittori, Alberto Vergara","doi":"10.4081/ijfs.2025.13563","DOIUrl":"https://doi.org/10.4081/ijfs.2025.13563","url":null,"abstract":"<p><p>The present study aimed to investigate the antimicrobial resistance (AMR) circulation through the different seasons in the Enterococcus genus isolated from mussels (Mytilus galloprovincialis) for human consumption and farmed along the coasts of the central Adriatic Sea (Abruzzo region, Italy). A total of 250 mussels were collected, and 32 Enterococci (90.62% Enterococcus faecium and 9.37% Enterococcus durans) were identified using the VITEK 2 system (bioMérieux, France). Antibiograms included 26 molecules used for the treatment of veterinary and human infections. Biomolecular screenings involved 45 genetic determinants responsible for AMR. Results showed mainly resistance against tetracycline (44.44%), vancomycin (27.78%), quinupristin-dalfopristin (16.67%), nitrofurantoin, and linezolid (11.11%). Concerning the antibiotic resistance genes (ARGs), multiplex end-point polymerase chain reaction assays mostly amplified tetC (59.37%), tetD (50.00%), cfr (43.75%), vanA and vanD (37.50%), vatE (21.87%), vatD, poxtA and qnrS (18.75%) and 52.67% and 35.11% in winter and spring seasons, respectively. The consistent environmental ARG circulation confirms the genetic pollution of marine environments, and the season variable (water temperatures) significantly influences their horizontal circulation and phenotypical expression. The AMR phenomenon, defined as uncontrolled, represents a crucial public health concern that needs to be monitored.</p>","PeriodicalId":14508,"journal":{"name":"Italian Journal of Food Safety","volume":" ","pages":""},"PeriodicalIF":1.8,"publicationDate":"2025-04-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143772398","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Andrea Zovi, Antonio Vitiello, Sauro Vittori, Germana Borsetta
{"title":"Physiological effects claimed for hemp seeds contained in food preparations: an update.","authors":"Andrea Zovi, Antonio Vitiello, Sauro Vittori, Germana Borsetta","doi":"10.4081/ijfs.2025.13653","DOIUrl":"https://doi.org/10.4081/ijfs.2025.13653","url":null,"abstract":"<p><p>Food supplements are one of the most widely marketed food categories in the world. In relation to several cases of adverse effects reported due to the plant substances contained in these products, the attention of the scientific communities and public decision-makers should be directed to the physiological effects that can be attributed to these substances and that encourage their increasing sales to consumers. This is the case of the plant Cannabis sativa L.. In order to evaluate the scientific validity of the physiological effects attributable to Cannabis sativa, a bibliographic search was carried out on the evidence published. This short manuscript has highlighted how, despite the presence on the market of a large number of plant substances present in food preparations, there is not always clear evidence to support the physiological effects attributed to these products. As a result, it is essential to monitor the market to safeguard the food safety of consumers worldwide.</p>","PeriodicalId":14508,"journal":{"name":"Italian Journal of Food Safety","volume":" ","pages":""},"PeriodicalIF":1.8,"publicationDate":"2025-03-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143752700","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Roberta Brunetti, Stefania Cavallo, Eloise Peirce, Roberta Pellicanò, Diletta Mandato, Francesca Romano, Ivana Elefante, Germana Colarusso, Pellegrino Fenizia, Federica Gargano, Loredana Baldi, Rosa D'Ambrosio
{"title":"Food safety and consumption patterns in the Campania region during the COVID-19 emergency.","authors":"Roberta Brunetti, Stefania Cavallo, Eloise Peirce, Roberta Pellicanò, Diletta Mandato, Francesca Romano, Ivana Elefante, Germana Colarusso, Pellegrino Fenizia, Federica Gargano, Loredana Baldi, Rosa D'Ambrosio","doi":"10.4081/ijfs.2025.13375","DOIUrl":"https://doi.org/10.4081/ijfs.2025.13375","url":null,"abstract":"<p><p>The objective of this study was to evaluate the association between consumers' experience of COVID-19 and changes in their food purchasing decisions during the lockdown as a result of their risk perception. An online questionnaire was created to analyze consumer purchasing behavior and awareness during the pandemic. At the end of the online administration period, the replies collected were downloaded. A descriptive analysis of the data was carried out through the construction of tables of individual variables; for each variable, the patterns, frequencies, and percentages with which they occurred were determined. Subsequently, a multiple correspondence analysis (MCA) was conducted; this is one of the most widely used statistical techniques for the \"multiple\" analysis of qualitative or mixed data collected through questionnaires. The final extraction of the completed questionnaires returned the replies of 114 individuals. In the MCA, not all the variables of the matrix were considered, as they were not proportional to the number of individuals. We considered 7 active variables and 1 supplementary variable, which contributed to the formation of factors. The most notable pattern was that those people who were most worried about the risk of contagion not only sanitized their purchases but also reduced their online purchases and deliveries, believing that these measures could reduce the risk of contagion.</p>","PeriodicalId":14508,"journal":{"name":"Italian Journal of Food Safety","volume":" ","pages":""},"PeriodicalIF":1.8,"publicationDate":"2025-03-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143735847","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}