Iranian Biomedical Journal最新文献_第7页

Metabolic Regulation of T cell Activity: Implications for Metabolic-Based T-cell Therapies for Cancer T细胞活性的代谢调节:基于代谢的T细胞治疗癌症的意义

Iranian Biomedical Journal Pub Date : 2023-01-01 DOI: 10.52547/ibj.3811

Jahangir Abdesheikhi, Farnaz Sedghy, Merat Mahmoodi, Hossein Fallah, Mahdi Ranjkesh

{"title":"Metabolic Regulation of T cell Activity: Implications for Metabolic-Based T-cell Therapies for Cancer","authors":"Jahangir Abdesheikhi, Farnaz Sedghy, Merat Mahmoodi, Hossein Fallah, Mahdi Ranjkesh","doi":"10.52547/ibj.3811","DOIUrl":"https://doi.org/10.52547/ibj.3811","url":null,"abstract":"Immunometabolism is an emerging field in tumor immunotherapy. Understanding the metabolic competition for access to the limited nutrients between tumor cells and immune cells can reveal the complexity of the tumor microenvironment and help develop new therapeutic approaches for cancer. Recent studies have focused on modifying the function of immune cells by manipulating their metabolic pathways. Besides, identifying metabolic events, which affect the function of immune cells leads to new therapeutic opportunities for treatment of inflammatory diseases and immune-related conditions. According to the literature, metabolic pathway such as glycolysis, tricarboxylic acid cycle, and fatty acid metabolism, significantly influence the survival, proliferation, activation, and function of immune cells and thus regulate immune responses. In this paper, we reviewed the role of metabolic processes and major signaling pathways involving in T-cell regulation and T-cell responses against tumor cells. Moreover, we summarized the new therapeutics suggested to enhance anti-tumor activity of T cells through manipulating metabolic pathways.","PeriodicalId":14500,"journal":{"name":"Iranian Biomedical Journal","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9971708/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10871147","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Multiplex Snapshot minisequencing for the detection of common PAH gene mutations in Iranian patients with Phenylketonuria 多重快照微序列检测伊朗苯丙酮尿症患者常见多环芳烃基因突变

Iranian Biomedical Journal Pub Date : 2023-01-01 DOI: 10.52547/ibj.3856

Pegah Namdar Aligoodarzi, Golale Rostami, Seyed Reza Kazemi Nezhad, Mohammad Hamid

{"title":"Multiplex Snapshot minisequencing for the detection of common PAH gene mutations in Iranian patients with Phenylketonuria","authors":"Pegah Namdar Aligoodarzi, Golale Rostami, Seyed Reza Kazemi Nezhad, Mohammad Hamid","doi":"10.52547/ibj.3856","DOIUrl":"https://doi.org/10.52547/ibj.3856","url":null,"abstract":"Background: Phenylketonuria is a common inborn defect of amino acid metabolism in the world. This failure is caused by an autosomal recessive insufficiency of the hepatic enzyme hyperphenylalaninemia (PAH), which catalyzes the irreversible hydroxylation of phenylalanine to tyrosine. More than 1,040 different disease-causing mutations have already been identified in the PAH gene. The most prominent complication of Phenylketonuria, if not diagnosed and treated, is severe mental retardation. Hence, early diagnosis and initiation of nutritional therapy are the most significant measures in preventing this mental disorder. Given these data, we developed a simple and rapid molecular test to detect the most frequent PAH mutations.Methods: Multiplex assay was developed based on the SNaPshot minisequencing approach to simultaneously perform genotyping of the 10 mutations at the PAH gene. We optimized detection of these mutations in one multiplex PCR, followed by 10 single-nucleotide extension reactions. DNA sequencing assay was also used to verify genotyping results obtained by SNaPshot minisequencing.Result: All 10 genotypes were determined based on the position and the fluorescent color of the peaks in a single electropherogram. Sequencing results of these frequent mutations showed that by using this method, a 100% detection rate could be achieved in the Iranian population.Conclusion: SNaPshot minisequencing can be useful as a secondary test in neonatal screening for HPA in neonates with a positive screening test, and it is also suitable for carrier screening. The assay can be easily applied for accurate and time- and cost-efficient genotyping of the selected SNPs in various population.","PeriodicalId":14500,"journal":{"name":"Iranian Biomedical Journal","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9971712/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10816098","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Rabies: Perils and Prevention, an Insight into Practices and Perception in Residents of Pakistan 狂犬病:危险与预防，巴基斯坦居民的实践与认知

Iranian Biomedical Journal Pub Date : 2023-01-01 DOI: 10.52547/ibj.3841

Saif Ullah Shaikh, Faryal Zaid, Zahida Shaikh, Muhammad Faisal Fahim

{"title":"Rabies: Perils and Prevention, an Insight into Practices and Perception in Residents of Pakistan","authors":"Saif Ullah Shaikh, Faryal Zaid, Zahida Shaikh, Muhammad Faisal Fahim","doi":"10.52547/ibj.3841","DOIUrl":"https://doi.org/10.52547/ibj.3841","url":null,"abstract":"Background: There is a sheer lack of knowledge in treating rabies in Pakistan. To decrease the number of victims every year, immunization and awareness programs are the basic necessities of Pakistani population. The aim of this study was to highlight the lack of learning strategies and how to overcome this problem, so as to eliminate rabies.Methods: This cross-sectional study was conducted on 692 respondents, aged 8-50 years, in Karachi city of Pakistan from January 2022 to June 2022. The study was based on demographic characteristics and basic knowledge of rabies, mode of transmission, clinical signs, and range of animal host species. Binary logistic regression analysis was performed to know the risk factor of rabies among different age groups, marital status, occupation, etc.Results: Results revealed that all the age groups were at risk of the wrong knowledge about rabies, odds = 1.182 and odds = 1.775 for 20-30 and 31-40 years of age, respectively; however, 31-40 years were at the high risk of showing odds=3.597 (95% C.I 1.621-7.983). The correlation of occupation was also checked with rabies knowledge. Only doctors (odds = 1.396) and students (odds = 1.955) showed their unawareness about rabies.Conclusion: This study highlights the grave situation that holds the country in the form of rabies. Through this study we aspire to raise awareness regarding the transmission, spread, and control of rabies","PeriodicalId":14500,"journal":{"name":"Iranian Biomedical Journal","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9971711/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10816102","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

In silico Identification of Hypoxic Signature followed by reverse transcription-quantitative PCR Validation in Cancer Cell Lines 肿瘤细胞系缺氧信号的计算机识别及逆转录-定量PCR验证

Iranian Biomedical Journal Pub Date : 2023-01-01 DOI: 10.52547/ibj.3803

Sara Shayan, Golnaz Bahramali, Arash Arashkia, Kayhan Azadmanesh

{"title":"In silico Identification of Hypoxic Signature followed by reverse transcription-quantitative PCR Validation in Cancer Cell Lines","authors":"Sara Shayan, Golnaz Bahramali, Arash Arashkia, Kayhan Azadmanesh","doi":"10.52547/ibj.3803","DOIUrl":"https://doi.org/10.52547/ibj.3803","url":null,"abstract":"Background: Hypoxic tumor microenvironment is one of the important impediments for conventional cancer therapy. This study aimed to computationally identify hypoxia-related messenger RNA (mRNA) signatures in nine hypoxic-conditioned cancer cell lines and investigate their role during hypoxia.Methods: Nine RNA sequencing (RNA-Seq) expression data sets were retrieved from the Gene Expression Omnibus database. Diﬀerentially expressed genes (DEGs) were identified in each cancer cell line. Then 23 common DEGs were selected by comparing the gene lists across the nine cancer cell lines. Reverse transcription-quantitative PCR (qRT-PCR) was performed to validate the identified DEGs.Results: By comparing the data sets, GAPDH, LRP1, ALDOA, EFEMP2, PLOD2, CA9, EGLN3, HK, PDK1, KDM3A, UBC, and P4HA1 were identified as hub genes. In addition, miR-335-5p, miR-122-5p, miR-6807-5p, miR-1915-3p, miR-6764-5p, miR-92-3p, miR-23b-3p, miR-615-3p, miR-124-3p, miR-484, and miR-455-3p were determined as common micro RNAs. Four DEGs were selected for mRNA expression validation in cancer cells under normoxic and hypoxic conditions with qRT-PCR. The results also showed that the expression levels determined by qRT-PCR were consistent with RNA-Seq data.Conclusion: The identified protein-protein interaction network of common DEGs could serve as potential hypoxia biomarkers and might be helpful for improving therapeutic strategies.","PeriodicalId":14500,"journal":{"name":"Iranian Biomedical Journal","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9971715/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10871150","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A Flow Cytometry Panel for Differential Diagnosis of Mantle Cell Lymphoma from Atypical B-Chronic Lymphocytic Leukaemia 流式细胞术鉴别诊断套细胞淋巴瘤与非典型b -慢性淋巴细胞白血病

Iranian Biomedical Journal Pub Date : 2023-01-01 DOI: 10.52547/ibj.3275

Mahdieh Mehrpouri, Maryam Sadat Hosseini, Leila Jafari, Mohammad Mosleh, Eesmaeil Shahabi Satlsar

{"title":"A Flow Cytometry Panel for Differential Diagnosis of Mantle Cell Lymphoma from Atypical B-Chronic Lymphocytic Leukaemia","authors":"Mahdieh Mehrpouri, Maryam Sadat Hosseini, Leila Jafari, Mohammad Mosleh, Eesmaeil Shahabi Satlsar","doi":"10.52547/ibj.3275","DOIUrl":"https://doi.org/10.52547/ibj.3275","url":null,"abstract":"Background: Differential diagnosis of chronic lymphoproliferative disorders (CLDs) has remained challenging due to the highly variable morphology features and immunophenotyping. Currently, the development of multiple-marker panel analyses by flow cytometry has opened a broad way for diagnosis of CLDs.Methods: We analyzed the peripheral blood and bone marrow samples of 131 patients with B-cell CLDs (including 91 chronic lymphocytic leukemia (CLL), 15 atypical CLL, 14 mantle cell lymphoma (MCL), and 11 CD5-/CD10-lymphoma patients) from April 2018 to April 2019, using a panel of specific markers by flow cytometry.Results: Our results indicated that the expression pattern of CD22, CD23, FMC-7, and CD5 allowed us to accurately and differentially diagnose the B-CLL, MCL, and CD5-/CD10- lymphoma, while it was not capable of differentiating MCL from atypical CLL. We, however, found that the expression patterns of CD38 and immunoglobulin light chain differed significantly between atypical B-CLL and MCL. CD38 and lambda light chain were remarkably expressed in MCL patients (92.8% and 85%, respectively) compared to the atypical CLL (1.1% and 0% respectively), with the p value less than 0.001 for both markers. In contrast to MCL patients, all the patients with atypical CLL, expressed kappa light chain. The immunohistochemistry method used for cyclin D1 confirmed that the flow cytometry detection of kappa and lambda light chains could provide a new approach with high sensitivity (91%) and moderate specificity (50%) to distinguish MCL patients from atypical B-CLL.Conclusion: Expression of CD5, CD20 (bright), CD22, FMC-7, CD38, and lambda light chain with no expression of CD23 can accurately detect MCL and differentiate it from atypical B-CLL","PeriodicalId":14500,"journal":{"name":"Iranian Biomedical Journal","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9971714/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10871149","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Preparation, Optimization, and Investigation of Naringenin-Loaded Microemulsion for Topical Application 外用柚皮素微乳的制备、优化及研究

Iranian Biomedical Journal Pub Date : 2022-11-01 DOI: 10.52547/ibj.3722

Anayatollah Salimi, Sara Amirimoghadam, Farid Bagheri

{"title":"Preparation, Optimization, and Investigation of Naringenin-Loaded Microemulsion for Topical Application","authors":"Anayatollah Salimi, Sara Amirimoghadam, Farid Bagheri","doi":"10.52547/ibj.3722","DOIUrl":"https://doi.org/10.52547/ibj.3722","url":null,"abstract":"Background: Flavonoids are a large group of phenolic compounds possessing anti-inflammatory and antioxidant effects. NAR is a flavonoid with various pharmacological properties. Using pharmaceutical compounds on skin is one of the routes of administration to achieve local and systemic effects. The aim of this study was to develop a topical formulation of NAR by the preparation of a NAR ME, which was further tested its skin permeability in rats.Methods: Eight 0.5% NAR MEs were prepared by mixing appropriate amounts of surfactant (Tween 80 and Labrasol), cosurfactant (Capryol 90) and the oil phase (oleic acid-Transcutol P in a ratio of 1:10). The drug was dissolved in the oil phase. The physicochemical properties of MEs such as droplet size, viscosity, release, and skin permeability were assessed using Franz Cells diffusion.Results: Based on the results, the droplet size of MEs ranged between 5.07 and 35.15 nm, and their viscosity was 164-291 cps. Independent factors exhibited a strong relationship with both permeability and drop size. The permeability findings revealed that the diffusion coefficient of NAR by the ME carrier increased compared to the drug saturation solution.Conclusion: The most validated results were obtained for Jss and particle size. Optimal formulations containing MEs with Jss and particle sizes varying between minimum and maximum amounts are suitable for topical formulations of NAR.","PeriodicalId":14500,"journal":{"name":"Iranian Biomedical Journal","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2022-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9763875/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10448313","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Combined Effect of Neutron Radiation and Curcumin on Breast Cancer Cells Cytotoxicity in 3D Culture Medium 中子辐射和姜黄素对三维培养基中乳腺癌细胞毒性的联合影响

Iranian Biomedical Journal Pub Date : 2022-11-01 DOI: 10.52547/ibj.3756

Sajedeh Zargan, Mehdi Salehi Barough, Jamil Zargan, Mohsen Shayesteh, Ashkan Haji Noor Mohammadi, Mohsen Mousavi, Hani Keshavarz Alikhani

{"title":"Combined Effect of Neutron Radiation and Curcumin on Breast Cancer Cells Cytotoxicity in 3D Culture Medium","authors":"Sajedeh Zargan, Mehdi Salehi Barough, Jamil Zargan, Mohsen Shayesteh, Ashkan Haji Noor Mohammadi, Mohsen Mousavi, Hani Keshavarz Alikhani","doi":"10.52547/ibj.3756","DOIUrl":"https://doi.org/10.52547/ibj.3756","url":null,"abstract":"Introduction: Introduction: Chemotherapy, biotherapy, and radiotherapy play a limited but important role in treating breast cancer. For more efficient treatment, combination therapy could be an appropriate option. In this study, radiotherapy using neutron radiation emitted from a 241Am-Be neutron source, as well as biotherapy using curcumin (80 μM) was combined to investigate the efficiency of treatment towards MCF-7 breast cancer in a three dimensional (3D) culture medium.Methods: Methods: MTT, neutral red uptake assay, nitric oxide, glutathione assay, catalase, cytochrome c, comet assay, and caspase-3 were used to determine the effect of neutron radiation and also neutron and curcumin combination on the viability of cancer cells.Results: Results: The results of cytotoxicity test showed that neutron irradiation with or without curcumin at 5, 10, 15, and 20 h reduced the survival of tumor cells. Moreover, the rate of apoptosis due to the neutron effect at different irradiation times enhanced with the increasing time.Conclusion: Conclusion: Due to the significant anticancer effect of curcumin in 3D culture, using this molecule before or after neutron therapy is recommended.","PeriodicalId":14500,"journal":{"name":"Iranian Biomedical Journal","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2022-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9841218/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10616300","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Evaluation of Anticancer and Cytotoxic Effects of Genistein on PC3 Prostate Cell Line under Three-Dimensional Culture Medium 染料木素对前列腺PC3细胞株三维培养的抗癌和细胞毒作用评价

Iranian Biomedical Journal Pub Date : 2022-11-01 DOI: 10.52547/ibj.3711

Seyedeh Masoumeh Khamesi, Mehdi Salehi Barough, Jamil Zargan, Mohsen Shayesteh, Nooshin Banaee, Ashkan Haji Noormohammadi, Hani Keshavarz Alikhani, Mohsen Mousavi

{"title":"Evaluation of Anticancer and Cytotoxic Effects of Genistein on PC3 Prostate Cell Line under Three-Dimensional Culture Medium","authors":"Seyedeh Masoumeh Khamesi, Mehdi Salehi Barough, Jamil Zargan, Mohsen Shayesteh, Nooshin Banaee, Ashkan Haji Noormohammadi, Hani Keshavarz Alikhani, Mohsen Mousavi","doi":"10.52547/ibj.3711","DOIUrl":"https://doi.org/10.52547/ibj.3711","url":null,"abstract":"Background: Prostate cancer is a major cause of disease and mortality among men. Genistein (GNT) is an isoflavone found naturally in legumes. Isoflavones, a subset of phytoestrogens, are structurally similar to mammalian estrogens. This study aimed to evaluate the anticancer and cytotoxic effects of GNT on PC3 cell line under three dimensional (3D) culture medium.Methods: The 3D culture was created by encapsulating the PC3 cells in alginate hydrogel. MTT assay, neutral red uptake, comet assay, and cytochrome C assay were used to study the anticancer and cytotoxic effects of GNT at 120, 240, and 480 μM concentrations. Also, nitric oxide (NO), catalase, and glutathione assay levels were determined to evaluate the effect of GNT on the cellular stress. The culture medium was used as the negative control.Results: GNT reduced the production of cellular NO and increased the production of catalase and glutathione, confirming the results of the NO test. Evaluation of the toxicity effect of GNT at the concentrations of 120, 240, and 480 μM using comet assay showed that this chemical agent induces apoptosis in PC3 cells in a dose-dependent manner. As the level of cytochrome C in PC3 cells treated with different concentrations of GNT was not significantly different from that of the control, GNT could induce apoptosis in PC3 cells through the non-mitochondrial pathway.Conclusion: The findings of this study disclose that the anticancer effect of GNT on PC3 cells under 3D culture conditions could increase the effectiveness of treatment. Also, the cell survival rate is dependent on GNT concentration.","PeriodicalId":14500,"journal":{"name":"Iranian Biomedical Journal","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2022-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9763873/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10814198","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 2

A Novel Hyperthermostable Recombinant Protein Nanocage 一种新型超热稳定重组蛋白纳米笼

Iranian Biomedical Journal Pub Date : 2022-11-01 DOI: 10.52547/ibj.3839

Yaghoub Ahmadyousefi, Massoud Saidijam, Bagher Amirheidari, Fatehmeh Rahbarizadeh, Meysam Soleimani

{"title":"A Novel Hyperthermostable Recombinant Protein Nanocage","authors":"Yaghoub Ahmadyousefi, Massoud Saidijam, Bagher Amirheidari, Fatehmeh Rahbarizadeh, Meysam Soleimani","doi":"10.52547/ibj.3839","DOIUrl":"https://doi.org/10.52547/ibj.3839","url":null,"abstract":"Background: Background: Ferritin has an important role in iron storage in the cells, and due to its nanocage structure and self-assembly properties, it has wide application prospects in nanobiotechnology.Methods: Methods: The maize (Zea mays) ferritin gene ZmFer1 was cloned and expressed in Escherichia coli BL21 (DE3) for the first time. Change in macromolecular structure of ZmFer1 ferritin due to heat treatment was investigated using native PAGE electrophoresis, dynamic light scattering (DLS), and transmission electron microscopy (TEM). Change in the secondary structures of the protein was evaluated using circular dichroism spectroscopy. Moreover, alteration in the conformation of the protein was evaluated using UV-absorption spectra and intrinsic fluorescence spectra. The melting temperature (Tm) of ZmFer1 was obtained using differential scanning calorimetry (DSC). Finally, the effect of heat on the function of ZmFer1 was assessed by iron loading ability.Results: Results: The purified ZmFer1 protein showed a homopolymer nanocage structure. The results of native PAGE electrophoresis, DLS, and TEM techniques showed that ZmFer1 protein nanocage is stable to heat treatment up to 90 °C, and some of the protein nanocages retain their macromolecular structures even at 100 °C in liquid aqueous solution. Based on the DSC results, ZmFer1 protein nanocage had a Tm of 81.9 °C. After treatment at 100 °C, stable ZmFer1 protein nanocages were able to store iron atoms.Conclusion: Conclusion: Recombinant ZmFer1 ferritin with a Tm > 80°C is a hyperthermostable protein nanocage. The results of this study are beneficial for the development of protein nanocages that are stable under extreme temperature conditions, as well as application of ZmFer1 in nanobiotechnology, biomaterials, and biomedical fields.","PeriodicalId":14500,"journal":{"name":"Iranian Biomedical Journal","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2022-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9841219/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10608641","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Upregulation of MiRNA-149-5p Reduces the Infract Volume in Middle Cerebral Artery Occlusion Rats by Modulating Cation-Chloride Cotransporters Expressions 上调MiRNA-149-5p通过调节阳离子-氯共转运蛋白表达减少大脑中动脉闭塞大鼠梗死体积

Iranian Biomedical Journal Pub Date : 2022-11-01 DOI: 10.52547/ibj.3759

Hossein Mostafavi, Narges Amoli, Elham Ghasemloo, Meysam Forouzandeh, Masoumeh Hosseini, Mehdi Eskandari

{"title":"Upregulation of MiRNA-149-5p Reduces the Infract Volume in Middle Cerebral Artery Occlusion Rats by Modulating Cation-Chloride Cotransporters Expressions","authors":"Hossein Mostafavi, Narges Amoli, Elham Ghasemloo, Meysam Forouzandeh, Masoumeh Hosseini, Mehdi Eskandari","doi":"10.52547/ibj.3759","DOIUrl":"https://doi.org/10.52547/ibj.3759","url":null,"abstract":"Background: Brain ischemia often leads to the chloride gradient alternations, which affects volume regulation and neuronal survival. Increase in NKCC1 expression and reduction in KCC2 level under ischemic condition results in inflammation and neuronal death. In this study, we investigated the effect of mimic miRNA and coenzyme Q10 (CoQ10) on the expression of cation-chloride cotransporters (CCCs) (NKCC1 and KCC2) after cerebral ischemia.Methods: In this study, cerebral ischemia was modeled using the middle cerebral artery occlusion method. Rats were randomly divided into six groups: sham, model, negative control, vehicle, and the first and second treatments. In the Sham group, ischemia was not induced, and no treatment was performed. In the Model group, ischemia induction was performed, and other groups, in addition to ischemia induction, received Scramble miRNA, Ethanol, mimic miRNA-149-5p and CoQ10, respectively. Each group was divided into three subgroups to assess the volume of the tissue damage and neurological deficits scores (NDS) in subgroup 1, brain water content in subgroup 2, level of miRNA-149-5p and CCC expressions in subgroup 3.Results: Our data suggested that the use of mimic miRNA and Q10 increased the level of miRNA-149 and KCC2 expression and decreased NDS, NKCC1 expression, brain water content, and infract volume.Conclusion: Findings of this study suggest that the mimic miRNA and Q10 may have neuroprotective effects through reducing infract volume and brain water content and modulating the expression of CCCs after brain ischemia.","PeriodicalId":14500,"journal":{"name":"Iranian Biomedical Journal","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2022-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9763874/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9233973","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0