International Journal of Secondary Metabolite最新文献_第6页

Chemical composition of essential oils from Crocus ancyrensis (Herbert) Maw Spreading In Çorum (Türkiye) Region 在Çorum（土耳其）地区传播的番红花（Herbert）Maw精油的化学成分

International Journal of Secondary Metabolite Pub Date : 2023-06-16 DOI: 10.21448/ijsm.1199416

H. Doğan, Ömer Kayir, Erol Alver, İ. Bi̇li̇ci̇

{"title":"Chemical composition of essential oils from Crocus ancyrensis (Herbert) Maw Spreading In Çorum (Türkiye) Region","authors":"H. Doğan, Ömer Kayir, Erol Alver, İ. Bi̇li̇ci̇","doi":"10.21448/ijsm.1199416","DOIUrl":"https://doi.org/10.21448/ijsm.1199416","url":null,"abstract":"Crocus ancyrensis is a yellow-flowered Crocus species and is in the same family and genus with saffron. Although various studies have been conducted on the antioxidant capacity and essential oil content characterization of saffron (Crocus sativus), there is no literature information about the C. ancyrensis plant, which is known as Crocus among the people. The members of Crocus family contain many valuable components including antioxidants, phenolic compounds and essential oils. The essential oils obtained from this family is a complex mixture of more than 30 components, which are primarily terpenes and their derivatives. These mixtures are used in paint, medicine, and food applications especially in the cosmetics sector. In the study, the essential oils of the Crocus ancyrensis plant were extracted with the clevenger system and characterized by GC-MS analyses. As a result, 23 volatile components were identified. 2-Hexenal, 1-ethylbutyl Hydroperoxide, 2-nitro-Hexane, β-Isophorone, α-Isophorone, 2-Caren-10-al and Eugenol are found as the main components of Crocus ancyrensis plant extract. Due to the antioxidant, antimicrobial, antifungal, anticancer and odorant properties of some of the identified components, C. ancyrensis can be used as a medicinal aromatic plant in various fields, especially in the cosmetics and perfume industry.","PeriodicalId":14437,"journal":{"name":"International Journal of Secondary Metabolite","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-06-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"47908479","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The effect of extraction methods on the yields, chemical composition and antifungal activity of sawdust Cedrus atlantica Manetti essential oils 研究了不同提取方法对木木屑精油产率、化学成分及抗真菌活性的影响

International Journal of Secondary Metabolite Pub Date : 2023-06-16 DOI: 10.21448/ijsm.1047122

Chaimaa Bouyahia, M. Benbouzi̇d, S. El hajjaji, M. Slaoui̇, F. Bentata, M. Labhi̇li̇li̇, A. Bouyahya

{"title":"The effect of extraction methods on the yields, chemical composition and antifungal activity of sawdust Cedrus atlantica Manetti essential oils","authors":"Chaimaa Bouyahia, M. Benbouzi̇d, S. El hajjaji, M. Slaoui̇, F. Bentata, M. Labhi̇li̇li̇, A. Bouyahya","doi":"10.21448/ijsm.1047122","DOIUrl":"https://doi.org/10.21448/ijsm.1047122","url":null,"abstract":"The aim of this work is to determine the effect of the extraction method on the yield, the chemical composition, and the antifungal activity of cedarwood essential oils (EOs) from sawdust of Moroccan Cedrus atlantica (C. atlantica). EOs were extracted by different methods: hydrodistillation, soxhlet, maceration, and ultrasound. The chemical composition was determined using gas chromatography mass spectrometry (GC-MS) analysis. The yields of EOs were 5.60%, 11.68%, 4.82% and 9.33% for hydrodistillation, soxhlet, maceration and ultrasound, respectively. GC-MS revealed a diversity of chemical compounds depending on extraction methods. Indeed, the main compound of EOs obtained by soxhlet, maceration, and ultrasound was copalic acid methyl ester by a rate of 28.41%, 20.24%, and 24.17%, respectively. However, -himachalene (21.32%) followed by -himachalene (9.40%), β-Copaen-4α-ol (7.71%) and longifolene (6.74%) are the main compounds of EO extracted by hydrodistillation. The antifungal activity of cedarwood EO was tested in vitro on two pathogenic fungi: Fusarium culmorum (F. Culmorum) and Botrytis cineria (B. Cinerea). The minimum inhibitory concentration (MIC) was determined by successive dilutions of the stock solutions. The extracted EOs by soxhlet, maceration and ultrasound showed the important inhibitory effect against B. cinerea (MIC=1.25 mL/L). However, F. culmorum showed resistance towards all tested EOs. The finding of this study showed clearly that the volatile composition of EOs can be variable according to extraction. methods. Moreover, antifungal effects are depending on chemical composition of EOs but also to tested staris.","PeriodicalId":14437,"journal":{"name":"International Journal of Secondary Metabolite","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-06-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41338966","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Ultrasonic assisted propolis extraction: characterization by ATR-FTIR and determination of its total antioxidant capacity and radical scavenging ability 超声波辅助蜂胶提取:用ATR-FTIR表征蜂胶提取工艺，测定其总抗氧化能力和自由基清除能力

International Journal of Secondary Metabolite Pub Date : 2023-06-16 DOI: 10.21448/ijsm.1167773

Ü. Erdoğan

{"title":"Ultrasonic assisted propolis extraction: characterization by ATR-FTIR and determination of its total antioxidant capacity and radical scavenging ability","authors":"Ü. Erdoğan","doi":"10.21448/ijsm.1167773","DOIUrl":"https://doi.org/10.21448/ijsm.1167773","url":null,"abstract":"In the current study, ultrasonic assisted ethanolic extract of propolis was discussed in detail, including their total phenolic content, total antioxidant capacity and radical scavenging capacity. For this purpose, we determined the total antioxidant capacity of propolis extract by CUPRAC and FRAP) assay. At the same time, the free radical scavenging capacity of propolis extracts was investigated via the DPPH• and CUPRAC- hydroxyl radical scavenging (HRS) methods. The chemical constituents of propolis extract were characterized by ATR-FTIR. The results revealed that propolis is rich in total phenolic components (189 mg GAE /g extract). According to the CUPRAC assay, the total antioxidant capacity of propolis extract was calculated to be 2.43 ± 0.07 mmol TE/g-propolis extract. FRAP value of propolis extract was determined as 1.11 mmol TE/g-propolis extract. DPPH• scavenging activity of propolis extract was calculated to be 0.71± 0.002 mmol TE / g - extract. On the other hand, according to the CUPRAC method, HRS capacity of propolis extract at different concentrations (5-10 μg/mL) was determined as 68.1% and 77.64 %, respectively. Research findings showed that propolis extract has a strong radical scavenging potential. The FTIR spectra of the functional groups originating from the phenolic compounds in the propolis extract were as expected.","PeriodicalId":14437,"journal":{"name":"International Journal of Secondary Metabolite","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-06-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"42799928","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Investigation of antimicrobial and anticancer activity of extracts obtained following UV application to Althaea officinalis L. callus cultures 紫外光对杜鹃花愈伤组织培养物的抑菌和抗癌活性研究

International Journal of Secondary Metabolite Pub Date : 2023-06-13 DOI: 10.21448/ijsm.1291929

F. A. Özdemir, Mesut Turan

{"title":"Investigation of antimicrobial and anticancer activity of extracts obtained following UV application to Althaea officinalis L. callus cultures","authors":"F. A. Özdemir, Mesut Turan","doi":"10.21448/ijsm.1291929","DOIUrl":"https://doi.org/10.21448/ijsm.1291929","url":null,"abstract":"Althaea officinalis L., a plant of the Malvaceae family, is widely used in alternative medicine. The aim of this study is to cultivate the Althaea officinalis plant under in vitro conditions to create an appropriate callus regeneration protocol and investigate the antimicrobial and anticancer activities of methanol and ethyl acetate extracts of calli after UV-C application. Leaf, petiole, and root parts of A. officinalis plants germinated in a sterile environment were used as explant sources. Explants were cultured on MS medium containing different concentrations of 2,4-D (1, 2 mg/l) and BAP (0.25, 0.50, 0.75 mg/l). The most effective (100%) callus growth and callus weight (516.24±0.48 mg) was observed on petiole explants using MS medium containing 1 mg/l 2,4-D + 0.25 mg/l BAP. Calli obtained from leaf and petiole explants were exposed to UV-C treatment. Extractions of calli were carried out using methanol and ethyl acetate solutions. 1 mg/ml, 5 mg/ml, and 10 mg/ml solutions of methanol and ethyl acetate extracts were prepared and their antimicrobial activity on bacteria was investigated using the disc diffusion method for 7 different gram-positive and 9 different gram-negative bacteria. None of the three extract concentrations used had any antimicrobial activities. The anticancer activities of the extracts on SH-SY5Y human neuroblastoma cells were studied using the WST-1 viability kit. 1000, 500, 250, 125, and 62.5 µg/ml concentrations of ethyl acetate extracts of leaf and petiole calli had anticancer activity.","PeriodicalId":14437,"journal":{"name":"International Journal of Secondary Metabolite","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-06-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"44107144","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Aroma profile of the essential oils from different parts of Pycnocycla aucherana Decne. ex Boiss. 圆叶青不同部位精油的香气特征。木香。

International Journal of Secondary Metabolite Pub Date : 2023-05-27 DOI: 10.21448/ijsm.1164925

Fatemeh ASKARİ, Fatemeh SEFİDKON, Zohreh EMAMİ BİSTGANİ, Mohamad Amin SOLTANİPOUR

{"title":"Aroma profile of the essential oils from different parts of Pycnocycla aucherana Decne. ex Boiss.","authors":"Fatemeh ASKARİ, Fatemeh SEFİDKON, Zohreh EMAMİ BİSTGANİ, Mohamad Amin SOLTANİPOUR","doi":"10.21448/ijsm.1164925","DOIUrl":"https://doi.org/10.21448/ijsm.1164925","url":null,"abstract":"The current study focused on the essential oil concentration and aroma profile in different parts of wild Pycnocycla aucherana Decne from Iran during two years 2017 and 2018. Plant samples of P. aucherana were collected from HajiAbad area located in Hormozgan province, Iran at reproductive stage on June 7, 2017 and 2018. Essential oil isolation was done by hydro-distillation method for 3 hours. For the qualification and quantification of components, gas chromatography/mass spectrometry (GC/MS) was applied. Results showed that the averages of essential oil percentage of shoot and leaf (SL) and seed in the first and second year were (0.13, 0.23%) and (0.4, 0.3%) respectively. The main chemotype was namely -phellandrene (5.96-16%), p-cymene (3.07-27.4%), Limonene (0.72-6.80%), γ-cadinene (0.8-4.33%), Spathulenol (1.90-8.64%), Elemol (0.3-6.69%), b-eudesmol (0.8-9.27), and Bulnesol (0.91-3.40%). The highest amount of -phellandrene (16%) and p-cymene (27.4%) was observed in the seed and (SL) of essential oils in the first year respectively. Elemol and Elemicin content increased during the second year in the seed of essential oils with amount of (6.69 %) and (25.69 %), respectively. Overall, the results showed that the geographic origin greatly influenced the chemical composition of P. aucherana.","PeriodicalId":14437,"journal":{"name":"International Journal of Secondary Metabolite","volume":"16 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-05-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135949908","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Antimicrobial evaluation of the Patchouli (Pogostemon cablin Benth.) leaf essential oil combination with standard antimicrobial compounds 广藿香(Pogostemon cablin Benth.)叶精油与标准抗菌化合物组合的抗菌评价

International Journal of Secondary Metabolite Pub Date : 2023-05-27 DOI: 10.21448/ijsm.1232606

Nazlı Şenay Beşi̇ri̇k, G. Göger

{"title":"Antimicrobial evaluation of the Patchouli (Pogostemon cablin Benth.) leaf essential oil combination with standard antimicrobial compounds","authors":"Nazlı Şenay Beşi̇ri̇k, G. Göger","doi":"10.21448/ijsm.1232606","DOIUrl":"https://doi.org/10.21448/ijsm.1232606","url":null,"abstract":"Pogostemon cablin Benth's leaves, which belong to the Lamiaceae family, are used to make patchouli essential oil (PEO). Sesquiterpenes are the main compounds in essential oil. Antibacterial, antifungal, antiviral, antiemetic, and anti-inflammatory activities are just a few of the many pharmacological actions that are well-known. In this investigation, cefuroxime (CEF), moxifloxacin (MOX), clarithromycin (CLA), fluconazole (FLU), and terbinafine (TER) were combined with patchouli (Pogostemon cablin leaf essential oil). Gram-positive Staphylococcus aureus ATCC 6538, Gram-negative Escherichia coli ATCC 8739, and yeasts Candida albicans ATCC 10231, and C. tropicalis ATCC 750 were tested against all combinations. Drug interaction was given as fractional inhibitory concentrations (FIC) after combinations of the essential oils were tested for their minimum inhibitory concentrations (MIC).\u0000The checkerboard method was used to assess antimicrobial interactions, Fluconazole, terbinafine, cefuroxime, and clarithromycin combined with essential oil demonstrated an \"additive effect\" against Candida strains together with E. coli and S. aureus.","PeriodicalId":14437,"journal":{"name":"International Journal of Secondary Metabolite","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-05-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"48728369","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Investigation of In Vitro antiproliferative activity properties of Spartium junceum L. (Spanish broom) against MDA-MB-231 and HepG2 cancer cell lines 菝葜(Spartium junceum L.)体外抗MDA-MB-231和HepG2癌细胞增殖活性的研究

International Journal of Secondary Metabolite Pub Date : 2023-05-27 DOI: 10.21448/ijsm.1182965

Fatma Tuğçe GÜRAĞAÇ DERELİ, S. Akkoç

{"title":"Investigation of In Vitro antiproliferative activity properties of Spartium junceum L. (Spanish broom) against MDA-MB-231 and HepG2 cancer cell lines","authors":"Fatma Tuğçe GÜRAĞAÇ DERELİ, S. Akkoç","doi":"10.21448/ijsm.1182965","DOIUrl":"https://doi.org/10.21448/ijsm.1182965","url":null,"abstract":"Cancer is among the top global public health burdens leading to millions of deaths each year. The study aims to investigate the antiproliferative effect of Spartium junceum L. flowers on different cancer cell lines. The ethanolic extract of the flowers was prepared in the present study. Phytochemical analysis of the plant extract revealed the presence of several phenolic compounds such as cinnamic acid and its derivatives (chlorogenic, p-coumaric, ferulic acids), protocatechuic acid, epicatechin and luteolin. This extract was tested against human breast (MDA-MB-231) and liver (HepG2) cancer cell lines to find out its antiproliferative activity. It was determined that the extract was effective against both cell lines with IC50 values of 2.37 ± 0.47 and 0.98 ± 0.01 µL/mL for MDA-MB-231 and HepG2, respectively. Particularly, the extract was found to be more effective in the liver cancer cell line than the breast cancer cell line. All these obtained findings led us to believe that this medicinal plant could be a promising antiproliferative agent candidate for the treatment of human liver and breast cancers.","PeriodicalId":14437,"journal":{"name":"International Journal of Secondary Metabolite","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-05-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"44436405","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Synergistic antibacterial evaluation of Coriandri aetheroleum and linalool with standard antibiotics 芫荽油、芳樟醇与标准抗生素的协同抑菌效果评价

International Journal of Secondary Metabolite Pub Date : 2023-05-27 DOI: 10.21448/ijsm.1140803

Gözde Öztürk, G. Göger, Fatih Demirci, B. Demirci

{"title":"Synergistic antibacterial evaluation of Coriandri aetheroleum and linalool with standard antibiotics","authors":"Gözde Öztürk, G. Göger, Fatih Demirci, B. Demirci","doi":"10.21448/ijsm.1140803","DOIUrl":"https://doi.org/10.21448/ijsm.1140803","url":null,"abstract":"Within this work, it was aimed to investigate the in vitro antibacterial properties of the Pharma grade coriander (Coriandrum sativum L.) essential oil, and its combinations. The chemical composition of the essential oil was confirmed by gas chromatography (GC) and gas chromatography-mass spectrometry (GC-MS) analyses, simultaneously. Furthermore, the potential antibacterial activity of both the essential oil, and (+)-linalool with standard compounds chloramphenicol, and ciprofloxacin were evaluated using an in vitro microdilution assay against a panel of selected pathogens, namely Acinetobacter baumannii, Bacillus cereus, Escherichia coli, Pseudomonas aeruginosa, and Staphylococcus aureus. Initially, the minimum inhibitory concentrations (MIC) were determined, the essential oil as well as (+)-linalool and the standard antibiotics were combined for the synergistic antibacterial activity potential, where the combination activities were expressed as fractional inhibitory concentration index values (Σ FIC). The Coriander oil was relatively more effective against B. cereus, P. aeruginosa, E. coli, A. baumannii (2500 µg/mL) compared to S. aureus (5000 µg/mL), respectively. (+)- Linalool was found as effective as the essential oil (2500-5000 µg/mL). Coriander oil and antibiotic combinations showed synergistic effects against B. cereus (∑FIC= 0.375), E. coli (∑FIC= 0.078) and S. aureus (∑FIC= 0.375). Combination with (+)-linalool, and antibiotics showed synergistic effects against B. cereus (∑FIC= 0.375), E. coli (∑FIC= 0.093), as well as against S. aureus (∑FIC= 0.375), respectively. To the best of our knowledge, this is the first time of the antimicrobial combination study of linalool, coriander oil, chloramphenicol, and ciprofloxacin. The initial findings of this work suggest further natural product and drug combination evaluations.","PeriodicalId":14437,"journal":{"name":"International Journal of Secondary Metabolite","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-05-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"47356289","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Phytochemical screening and in vitro antioxidant activities of Mentha suaveolens Ehrh. extract 薄荷的植物化学筛选及体外抗氧化活性。摘录

International Journal of Secondary Metabolite Pub Date : 2023-05-27 DOI: 10.21448/ijsm.1148664

Moha Afrokh, K. Boumhara, K. Chatoui̇, S. Tahrouch, A. Hati̇mi̇, H. Harhar, M. Tabyaoui

{"title":"Phytochemical screening and in vitro antioxidant activities of Mentha suaveolens Ehrh. extract","authors":"Moha Afrokh, K. Boumhara, K. Chatoui̇, S. Tahrouch, A. Hati̇mi̇, H. Harhar, M. Tabyaoui","doi":"10.21448/ijsm.1148664","DOIUrl":"https://doi.org/10.21448/ijsm.1148664","url":null,"abstract":"Within the framework of the valorization of the medicinal and aromatic plants of Morocco, we were interested during this study in the characterization and the phytochemical identification of some secondary metabolites present in Mentha suaveolens Ehrh. and the evaluation of the antioxidant activity of this species using four methods: DPPH free radical scavenging test, 2,2'-azinobis-(3-ethylbenzothiazoline-6-sulfonic acid, (ABTS) radical cation scavenging test, FRAP test measuring antioxidant power, and β bleaching-carotene. Mentha suaveolens is a species belonging to the Lamiaceae family, harvested in southern Morocco, and widely used in traditional medicine for its biological properties attributed mainly to phenolic compounds. Just as they protect plants, secondary metabolites also have a protective role in the human body and are therefore beneficial to our health. They are attributed, in particular, with antioxidant, anti-inflammatory, and antibacterial properties. In this regard, characterization and identification tests of secondary metabolites revealed the presence of alkaloids, flavonoids, catechic tannins, and terpenes in this plant, whereas, the aerial parts of this species are devoid of coumarins, cyanogenic compounds, saponins and free quinones. The quantification of the phenolic compounds gave high contents, with contents of total phenols (54.75 ± 5.62) μg GAE/mg, total flavonoids (32.41 ± 0.41) μg QE/mg, and total condensed tannins (27 ± 1) μg CE/mg. In addition, the results obtained show that Mentha suaveolens extract has stronger antioxidant activity using the β-Carotene method with IC50 (0.24 ± 0.06) mg/mL against IC50 (0.021 ± 0.001) mg/mL for the standard antioxidant by the DPPH free radical scavenging test.","PeriodicalId":14437,"journal":{"name":"International Journal of Secondary Metabolite","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-05-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"43638174","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

In-vitro anti-diabetic, anti-Alzheimer, anti-tyrosinase, antioxidant activities of selected coumarin and dihydroisocoumarin derivatives 香豆素和二氢异香豆素衍生物的体外抗糖尿病、抗阿尔茨海默病、抗酪氨酸酶、抗氧化活性

International Journal of Secondary Metabolite Pub Date : 2023-05-24 DOI: 10.21448/ijsm.1196712

H. Şahi̇n

{"title":"In-vitro anti-diabetic, anti-Alzheimer, anti-tyrosinase, antioxidant activities of selected coumarin and dihydroisocoumarin derivatives","authors":"H. Şahi̇n","doi":"10.21448/ijsm.1196712","DOIUrl":"https://doi.org/10.21448/ijsm.1196712","url":null,"abstract":"Benzo-α-pyrone structured coumarin derivatives are secondary metabolites first obtained from Coumarouna odorata in 1822. Coumarin and its structural isomer dihydroisocoumarin derivatives are found in many different sources in nature. Several different bioactivities of these compounds have been reported. In this study, preliminary activity screening and comparison of four purchased coumarin derivatives (esculetin, esculin monohydrate, umbelliferon, scoparone) and four previously isolated 3-phenyl-3,4-dihydroisocoumarin derivatives (thunberginol C, scorzocreticoside I, scorzocreticoside II, scorzopygmaecoside) from a medicinal plant were carried out by in-vitro methods. α-Glucosidase, acetylcholinesterase, butyrylcholinesterase, tyrosinase inhibitor activities and antioxidant potentials of the compounds were evaluated. Consequently, thunberginol C (free – not glycosylated form of 3,4-dihydroisocoumarin structure) showed better potential in all enzyme inhibitory activities compared to coumarin structure. Particularly, α-glucosidase inhibitory activity of this compound with a very low IC50 value (94.76±2.98 µM) compared to standard acarbose (1036.2±2.70 µM) should be noted. Glycosylation and/or methoxy substitution of 3,4-dihydroisocoumarin structure resulted a significant decrease in all tested enzyme inhibitory activities. The structures of esculin MH, umbelliferone, scoparone, scorzocreticoside I, and scorzopygmaeceoside might be considered in further synthetic studies as selective acetylcholinesterase inhibitors. Thunberginol C has a promising potential in tyrosinase inhibitory activity. Esculetin and thunberginol C showed the best results with high potentials in antioxidant activity via 2,2-diphenyl-1-picryl-hydrazyl-hydrate free radical scavenging, 2,2′-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid cation radical decolorization, and cupric ion reducing antioxidant capacity assays compared to the standards.","PeriodicalId":14437,"journal":{"name":"International Journal of Secondary Metabolite","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-05-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"45169825","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1