超声波辅助蜂胶提取:用ATR-FTIR表征蜂胶提取工艺,测定其总抗氧化能力和自由基清除能力

Ü. Erdoğan
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引用次数: 1

摘要

本研究对超声波辅助蜂胶乙醇提取物进行了详细的研究,包括其总酚含量、总抗氧化能力和自由基清除能力。为此,我们通过CURAC和FRAP)测定法测定了蜂胶提取物的总抗氧化能力。同时,采用DPPH•和CUTRAC-羟基自由基清除(HRS)方法研究了蜂胶提取物对自由基的清除能力。用ATR-FTIR对蜂胶提取物的化学成分进行了表征。结果表明,蜂胶富含总酚类成分(189mg GAE/g提取物)。根据CURAC测定,蜂胶提取物的总抗氧化能力为2.43±0.07mmol TE/g-蜂胶提取物。蜂胶提取物的FRAP值为1.11mmol TE/g-蜂胶提取物。蜂胶提取物的DPPH•清除活性为0.71±0.002mmol TE/g提取物。另一方面,根据CURAC方法,不同浓度(5-10μg/mL)的蜂胶提取物的HRS容量分别为68.1%和77.64%。研究结果表明,蜂胶提取物具有较强的清除自由基的潜力。蜂胶提取物中源自酚类化合物的官能团的FTIR光谱如预期的那样。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Ultrasonic assisted propolis extraction: characterization by ATR-FTIR and determination of its total antioxidant capacity and radical scavenging ability
In the current study, ultrasonic assisted ethanolic extract of propolis was discussed in detail, including their total phenolic content, total antioxidant capacity and radical scavenging capacity. For this purpose, we determined the total antioxidant capacity of propolis extract by CUPRAC and FRAP) assay. At the same time, the free radical scavenging capacity of propolis extracts was investigated via the DPPH• and CUPRAC- hydroxyl radical scavenging (HRS) methods. The chemical constituents of propolis extract were characterized by ATR-FTIR. The results revealed that propolis is rich in total phenolic components (189 mg GAE /g extract). According to the CUPRAC assay, the total antioxidant capacity of propolis extract was calculated to be 2.43 ± 0.07 mmol TE/g-propolis extract. FRAP value of propolis extract was determined as 1.11 mmol TE/g-propolis extract. DPPH• scavenging activity of propolis extract was calculated to be 0.71± 0.002 mmol TE / g - extract. On the other hand, according to the CUPRAC method, HRS capacity of propolis extract at different concentrations (5-10 μg/mL) was determined as 68.1% and 77.64 %, respectively. Research findings showed that propolis extract has a strong radical scavenging potential. The FTIR spectra of the functional groups originating from the phenolic compounds in the propolis extract were as expected.
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来源期刊
International Journal of Secondary Metabolite
International Journal of Secondary Metabolite Biochemistry, Genetics and Molecular Biology-Biotechnology
CiteScore
1.40
自引率
0.00%
发文量
28
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