International Journal of Mycobacteriology最新文献_第10页

Potential Role of Whole Genome Sequencing to Predict the Virulence, Anti-TB Resistance, and Variants of Mycobacterium tuberculosis Strains from Rifampicin-sensitive Pulmonary Tuberculosis Patients in Surabaya, East Java, Indonesia. 全基因组测序在预测印度尼西亚东爪哇泗水利福平敏感肺结核患者结核分枝杆菌菌株的毒力、抗结核耐药性和变体中的潜在作用

IF 1.6

International Journal of Mycobacteriology Pub Date : 2025-04-01 Epub Date: 2025-06-20 DOI: 10.4103/ijmy.ijmy_54_25

Ni Made Mertaniasih, Muhamad Frendy Setyawan, Ummi Amaliatush Sholichah Putri Merdekawati, Nurul Wiqoyah, Deby Kusumaningrum, Irfan Arif Ikhwani, Sohkichi Matsumoto

{"title":"Potential Role of Whole Genome Sequencing to Predict the Virulence, Anti-TB Resistance, and Variants of Mycobacterium tuberculosis Strains from Rifampicin-sensitive Pulmonary Tuberculosis Patients in Surabaya, East Java, Indonesia.","authors":"Ni Made Mertaniasih, Muhamad Frendy Setyawan, Ummi Amaliatush Sholichah Putri Merdekawati, Nurul Wiqoyah, Deby Kusumaningrum, Irfan Arif Ikhwani, Sohkichi Matsumoto","doi":"10.4103/ijmy.ijmy_54_25","DOIUrl":"10.4103/ijmy.ijmy_54_25","url":null,"abstract":"Background: Tuberculosis (TB) is the second concern of a fatal infectious disease in the world caused by Mycobacterium tuberculosis (MTB). Indonesia has many regions that is known as a hotspot region for MTB cases, one of the most cities high newly case detected in 5 years was Surabaya. In 2022, Surabaya reported a higher pulmonary TB (PTB) prevalence rate of 0.35%. This study aimed to investigate the genomic and phylogenetic characteristics of MTB from isolates of rifampicin-sensitive PTB patients in Surabaya using whole genome sequencing (WGS).Methods: This study is a cross-sectional study to descriptively analyses WGS data using bioinformatics. Out of 8 enrolled drug-sensitive PTB patients; however, only three cultured isolates successfully grew on MB 7H11/OADC agar and subjected for WGS analysis.Results: Whole genome analysis revealed that all the samples were drug sensitive. The identified samples were majority belonged to lineage 4.4.1 (Euro-American [S-type]) and we found a novel strain in East Java region known as Lineage 4.10 (Euro-American [Uganda 1]). In addition, we identified a novel SNVs predicted to be associated with genomic adaptation in fgd1, embC, embA, and rv0565c under antibiotic pressures.Conclusion: WGS predicts that all the samples from pulmonary rifampicin-sensitive TB patients in this study were drug sensitive. We report the first discovery of a novel L4.10 strain, classified as Uganda 1, in Surabaya, Indonesia.","PeriodicalId":14133,"journal":{"name":"International Journal of Mycobacteriology","volume":"14 2","pages":"153-163"},"PeriodicalIF":1.6,"publicationDate":"2025-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144336510","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Nonfunctioning Kidney Due to Renal Tuberculosis: A Diagnostic Challenge. 肾结核导致肾功能不全：诊断挑战。

IF 1.6

International Journal of Mycobacteriology Pub Date : 2025-04-01 Epub Date: 2025-06-20 DOI: 10.4103/ijmy.ijmy_46_24

Madhuri Singh, Shirish Sahebrao Chandanwale, Akshi Raj, Kumar Roushan, Anuj Sharma

{"title":"Nonfunctioning Kidney Due to Renal Tuberculosis: A Diagnostic Challenge.","authors":"Madhuri Singh, Shirish Sahebrao Chandanwale, Akshi Raj, Kumar Roushan, Anuj Sharma","doi":"10.4103/ijmy.ijmy_46_24","DOIUrl":"10.4103/ijmy.ijmy_46_24","url":null,"abstract":"Urogenital tuberculosis (TB) is a common manifestation of extrapulmonary TB, accounting for approximately 30%-40% of all cases, with the kidneys being the most frequently affected organ. Despite its prevalence, renal TB often presents diagnostic challenges due to nonspecific clinical symptoms, which can lead to delayed diagnosis and treatment. Increased occurrences of extrapulmonary TB have been observed in recent decades, linked to a rise in organ transplants and the prevalence of acquired immune deficiency syndrome. The urogenital form of the disease may arise from either disseminated infection or primary genitourinary localization. Symptoms typically include pyuria, dysuria, fever, flank pain, and burning micturition, often revealing a mass related to hydronephrosis of the affected kidney. Clinicians in regions with high TB prevalence, such as India, should maintain a high index of suspicion for renal TB, especially in patients with recurrent urinary tract infections. Early identification and treatment are crucial to prevent the development of nonfunctioning kidneys and associated complications. This case report highlights the importance of recognizing the clinical presentation of renal TB to improve diagnosis and management in affected patients.","PeriodicalId":14133,"journal":{"name":"International Journal of Mycobacteriology","volume":"14 2","pages":"201-203"},"PeriodicalIF":1.6,"publicationDate":"2025-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144336509","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Re-evaluated Treatment Outcomes of Bacteriologically Positive Patients with Tuberculosis Registered at an Urban Health Center, Lusaka, Zambia, 2023. 2023年在赞比亚卢萨卡城市卫生中心登记的细菌学阳性结核病患者的治疗效果重新评估

IF 1.5

International Journal of Mycobacteriology Pub Date : 2025-01-01 Epub Date: 2025-03-20 DOI: 10.4103/ijmy.ijmy_232_24

Samuel Daka, Masaki Ota, Graham Kapalu Samungole

{"title":"Re-evaluated Treatment Outcomes of Bacteriologically Positive Patients with Tuberculosis Registered at an Urban Health Center, Lusaka, Zambia, 2023.","authors":"Samuel Daka, Masaki Ota, Graham Kapalu Samungole","doi":"10.4103/ijmy.ijmy_232_24","DOIUrl":"10.4103/ijmy.ijmy_232_24","url":null,"abstract":"Background: The objective is to re-evaluate treatment outcomes of all bacteriologically positive patients with tuberculosis (TB) registered at Facility X in 2023.Methods: A retrospective cohort study was conducted on treatment outcomes of patients with TB using TB treatment and laboratory TB registers and treatment cards. Patients with incorrect treatment outcomes and those who were not evaluated by the facility had correct treatment outcomes assigned. In addition, treatment outcomes for patients who were transferred out were retrieved.Results: A total of 350 patients with bacteriologically positive TB were registered at facility X in 2023. The number of male patients was 274 (78%), the age of the patients ranged from 2.9 to 80 years, and 262 (74.9%) were new patients. The health facility managed to evaluate 340 (97%) patients, of whom 334 (95.4%) were correctly evaluated, while 16 (4.6%) were either incorrectly evaluated or not evaluated at all. As a result of this re-evaluation, the proportion of the evaluated patients increased from 97% (340 of 350 patients) to 99.4% (348 of 350 patients). The cure rate rose from 90.6% to 92.2% and the treatment success rate increased from 90.9% to 92.9%.Conclusion: This study has demonstrated that it is possible to evaluate almost all patients with TB at the end of their treatment. Inaccuracies in reporting TB data can negatively affect the implementation of TB programs. Health facilities should strive to correctly evaluate all patients with TB.","PeriodicalId":14133,"journal":{"name":"International Journal of Mycobacteriology","volume":"14 1","pages":"43-47"},"PeriodicalIF":1.5,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145280294","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Treatment Adherence among People with Drug-resistant Tuberculosis in Lagos Nigeria: The Effects of Stigma, Resilience, Social Support, and Temporal Discounting. 尼日利亚拉各斯耐药结核病患者的治疗依从性：耻辱感、恢复力、社会支持和时间折扣的影响。

IF 1.5

International Journal of Mycobacteriology Pub Date : 2025-01-01 Epub Date: 2025-03-20 DOI: 10.4103/ijmy.ijmy_209_24

Olusola Adedeji Adejumo, Olusoji James Daniel, Champaklal Jinabhai, Firoza Haffejee

{"title":"Treatment Adherence among People with Drug-resistant Tuberculosis in Lagos Nigeria: The Effects of Stigma, Resilience, Social Support, and Temporal Discounting.","authors":"Olusola Adedeji Adejumo, Olusoji James Daniel, Champaklal Jinabhai, Firoza Haffejee","doi":"10.4103/ijmy.ijmy_209_24","DOIUrl":"10.4103/ijmy.ijmy_209_24","url":null,"abstract":"Background: This study assessed the effects of social support, resilience, temporal discounting, and stigma on medication adherence among people with drug-resistant tuberculosis (PwDR-TB) in Lagos, Nigeria.Methods: A cross-sectional study was conducted between September and December 2023 among 203 adults on DR-TB treatment. The Morisky Medication Adherence Scale-8, Redwood DR-TB scale, multidimensional scale of perceived social support, brief resilience scale, and deferment of gratification scale were used to assess adherence, stigma, social support, resilience, and temporal discounting respectively. Pearson's correlation and hierarchical linear regression analysis were conducted to explore the relationships between adherence, stigma, social support, resilience, and temporal discounting.Results: The prevalence of low, medium, and high adherence was 20.7%, 73.4%, and 5.9%, respectively. Adherence was positively associated with social support (B = 0.380, P < 0.001), resilience (B = 0.210, P < 0.001), and temporal discounting (B = 0.364, 0 < 0.001) and negatively associated with stigma (B = -0.317, P < 0.001). Temporal discounting made a higher significant contribution (B = 0.343, P < 0.001) in predicting adherence than social support (B = 0.187, P = 0.005), resilience (B = 0.175, P = 0.002) and stigma (B = -0.317, P < 0.001).Conclusion: Patient-centred interventions that promote social support, resilience, and temporal discounting are urgently needed to enhance adherence among PwDR-TB. Stigma reduction strategies are required at all levels.","PeriodicalId":14133,"journal":{"name":"International Journal of Mycobacteriology","volume":"14 1","pages":"36-42"},"PeriodicalIF":1.5,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145280279","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Disseminated Mycobacterium haemophilum Infection after Antitumor Necrosis Factor Therapy, Misdiagnosed as Leprosy. 抗肿瘤坏死因子治疗后弥散性血友病分枝杆菌感染误诊为麻风病。

IF 1.5

International Journal of Mycobacteriology Pub Date : 2025-01-01 Epub Date: 2025-03-20 DOI: 10.4103/ijmy.ijmy_5_25

Alireza Eskandari, Mitra Rezaei, Afshin Moniri, Majid Marjani

引用次数: 0

In Memoriam: Professor Leonid P. Titov (1946-2025). 纪念：列昂尼德·p·蒂托夫教授（1946-2025）。

IF 1.5

International Journal of Mycobacteriology Pub Date : 2025-01-01 Epub Date: 2025-03-20 DOI: 10.4103/ijmy.ijmy_31_25

Ali Akbar Velayati, Parissa Farnia

引用次数: 0

Hematemesis Leading to Diagnosis of Pulmonary Aspergillosis. 呕血导致肺曲菌病的诊断。

IF 1.5

International Journal of Mycobacteriology Pub Date : 2025-01-01 Epub Date: 2025-03-20 DOI: 10.4103/ijmy.ijmy_20_25

Mazin Mohammed Al-Mubasher, Tarig Hakim Merghani, Rana El Beshbeishy, Azza Osman Alawad

引用次数: 0

Pyrrolo[1,2-a]quinoxalinic Core-Derived Compounds Targeting the Mycobacterium tuberculosis P-type ATPase Plasma Membrane Ca2+ Transporter, CtpF, as Potential Antituberculous Drugs. 靶向结核分枝杆菌p型atp酶质膜Ca2+转运体（CtpF）的吡咯罗[1,2-a]喹啉核心衍生化合物作为潜在的抗结核药物。

IF 1.5

International Journal of Mycobacteriology Pub Date : 2025-01-01 Epub Date: 2025-03-20 DOI: 10.4103/ijmy.ijmy_197_24

Michael Daniela Rodriguez-Afanado, Fabian Lopez-Vallejo, Cristian Ochoa-Puentes, Luz M Salazar, Carlos Yesid Soto

{"title":"Pyrrolo[1,2-a]quinoxalinic Core-Derived Compounds Targeting the Mycobacterium tuberculosis P-type ATPase Plasma Membrane Ca2+ Transporter, CtpF, as Potential Antituberculous Drugs.","authors":"Michael Daniela Rodriguez-Afanado, Fabian Lopez-Vallejo, Cristian Ochoa-Puentes, Luz M Salazar, Carlos Yesid Soto","doi":"10.4103/ijmy.ijmy_197_24","DOIUrl":"10.4103/ijmy.ijmy_197_24","url":null,"abstract":"Background: The resistance of Mycobacterium tuberculosis (Mtb) on first- and second-line anti-tuberculosis (TB) drugs is an issue for TB control; therefore, developing new anti-TB drugs is a priority in TB research. In this sense, the Ca2+ P-type ATPase plasma membrane transporter CtpF is an interesting anti-TB drug target.Methods: In this work, the activity of 4C-substituted pyrrolo[1,2-a]quinoxalinic compounds on Mtb viability and Ca2+ ATPase activity mediated by the plasma membrane transporter, CtpF, was assessed. The pyrrolo[1,2-a]quinoxalines compounds were initially in silico and analyzed as potential inhibitors of the CtpF transporter. Molecular docking analyses showed that 4-(3,4-methylenedioxyphenyl) pyrrolo[1,2-a]quinoxaline (4b) and 4-(2-chlorophenyl) pyrrolo[1,2-a]quinoxaline (4c) compounds are potential CtpF inhibitors. These compounds were synthesized by green chemistry using deep eutectic solvent under environmentally friendly processes.Results: Even though both compounds, 4b and 4c, inhibit the plasma membrane Ca2+ ATPase activity mediated by the CtpF transporter (IC50 of 8.05 ± 0.04 µM and 9.15 ± 0.03 µM for 4b and 4c, respectively), only the 4b compound was active on Mtb cells (MIC = 25 µg/mL). Interestingly, compound 4b also showed low toxicity on VERO cells (19.65 ± 0.51%) and hemolytic activity (1.45 ± 0.20%) in human O Rh (+) erythrocytes.Conclusions: 4-(3,4-methylenedioxyphenyl) pyrrolo[1,2-a]quinoxalinic core-derived compounds could be useful for developing alternative anti-TB compounds.","PeriodicalId":14133,"journal":{"name":"International Journal of Mycobacteriology","volume":"14 1","pages":"10-23"},"PeriodicalIF":1.5,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145280297","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Tubercular Vasculitis: A Rare Occurrence. 结核性血管炎：一种罕见的疾病。

IF 1.5

International Journal of Mycobacteriology Pub Date : 2025-01-01 Epub Date: 2025-03-20 DOI: 10.4103/ijmy.ijmy_218_24

Abhijit Acharya, Anusha Preethi, Shakti Bedanta Mishra, Sagarika Panda, Sumirini Puppala

引用次数: 0

Evaluation of Fluorescein Diacetate Viability Staining during Treatment Follow-up Examinations of Smear Positive Pulmonary Tuberculosis Patients. 双醋酸荧光素活性染色在涂阳肺结核患者治疗随访检查中的评价。

IF 1.5

International Journal of Mycobacteriology Pub Date : 2025-01-01 Epub Date: 2025-03-20 DOI: 10.4103/ijmy.ijmy_220_24

Ujjwala Nitin Gaikwad, Padma Das, Anudita Bhargava, Ajoy Kumar Behera, Tushar Bharat Jagzape, Sachin Chandraker

{"title":"Evaluation of Fluorescein Diacetate Viability Staining during Treatment Follow-up Examinations of Smear Positive Pulmonary Tuberculosis Patients.","authors":"Ujjwala Nitin Gaikwad, Padma Das, Anudita Bhargava, Ajoy Kumar Behera, Tushar Bharat Jagzape, Sachin Chandraker","doi":"10.4103/ijmy.ijmy_220_24","DOIUrl":"10.4103/ijmy.ijmy_220_24","url":null,"abstract":"Background: Assessment of treatment response to pulmonary tuberculosis (TB) is currently done by follow-up cultures since smear microscopy cannot distinguish viable from nonviable bacilli. This delays the therapeutic decision and increases the work load on the TB laboratories. This study evaluated the utility of fluorescein diacetate (FDA) viability staining against culture as a modality to assess treatment response in patients receiving anti tubercular treatment.Methods: In a cross-sectional study conducted for 2 years at a tertiary care hospital, around 110 follow-up sputum samples from patients with initial smear positivity were collected. After being processed for light-emitting diode fluorescence (FL) microscopy and liquid culture by MGIT 960, the samples were subjected to FDA viability staining as an additional step. FDA staining and FL microscopy results were compared to culture to determine its diagnostic accuracy in predicting bacterial viability.Results: The positivity rates for FL microscopy, FDA microscopy, and culture were 17%, 10%, and 8.18%, respectively. Culture results correlated with FL microscopy in 83.6% of cases and with FDA staining in 94.5%. FDA staining outperformed FL microscopy in terms of sensitivity, specificity, positive predictive value (PPV), and NPV, with values of 77.7%, 96.04%, 63.6%, and 97.9%, respectively. Among FL microscopy positive samples, FDA staining demonstrated 100% sensitivity and NPV, but poor specificity and PPV (57.14% and 45.45%, respectively) owing to false positive results in some cases.Conclusion: FDA viability staining proved to be a better predictor of bacteriological clearance during follow-up examinations of patients on antitubercular therapy and may be explored in future research.","PeriodicalId":14133,"journal":{"name":"International Journal of Mycobacteriology","volume":"14 1","pages":"30-35"},"PeriodicalIF":1.5,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145280206","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0