International Journal of General Medicine最新文献

{"title":"Association of a Sulfur-Containing Diet and a CTH Polymorphism with Bone Density in the Uyghur Population of China: A Preliminary Study.","authors":"Lingna Fang, Zhiqin Zhang, Dawei He, Yanming Hao, Yan Gao, Rongzhu Lu, Chong Li","doi":"10.2147/IJGM.S522804","DOIUrl":"10.2147/IJGM.S522804","url":null,"abstract":"<p><strong>Background: </strong>The aim of this study was to examine the impact of a sulfur-containing diet and a CTH polymorphism (G1208T; rs1021737) on bone density.</p><p><strong>Methods: </strong>A total of 200 Uyghur residents aged over 50 from Xinjiang, China, were recruited for this study. Fasting blood samples were collected from the participants to measure serum hydrogen sulfide (H₂S) levels and perform CTH polymorphism sequencing. Dietary sulfur intake was assessed using a food frequency questionnaire and categorized into animal protein-derived and non-animal protein-derived sources. Skeletal health of the calcaneus was evaluated using quantitative ultrasound scanning.</p><p><strong>Results: </strong>The study included a total of 200 participants, comprising 81 males and 119 females. Participants were stratified based on osteopenia status, with a T-score ≥ -1.0 indicating normal bone density and a T-score < -1.0 indicating osteopenia. Individuals in the osteopenia group exhibited significantly lower bone density markers, including broadband ultrasound attenuation (BUA) and speed of sound (SOS), as well as lower total weekly dietary sulfur intake and weekly dietary sulfur intake from animal protein. Additionally, they had significantly higher serum H₂S levels compared to participants with normal bone density. However, no differences in CTH genotype were observed between the normal bone density group and the osteopenia group. Participants were further categorized into tertiles (Q1 to Q3) based on weekly dietary sulfur intake from animal protein. Compared to the Q1 group, the Q3 group showed significantly higher T-scores and BUA values. Binary logistic regression analysis revealed that, compared to the group with high weekly dietary sulfur intake from animal protein, the low and middle intake groups had 3.252 times and 4.330 times higher risks of osteopenia, respectively.</p><p><strong>Conclusion: </strong>Dietary sulfur intake from animal protein may exert a protective effect on bone density.</p>","PeriodicalId":14131,"journal":{"name":"International Journal of General Medicine","volume":"18 ","pages":"2901-2910"},"PeriodicalIF":2.1,"publicationDate":"2025-06-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12151083/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144266177","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The Prognostic Significance of TMSB4X in Glioma Patients.","authors":"Sijie Li, Tianyi Fan, Zengyao Hao, Zizhou Zhang, Xuetao Han, Jiayuan Li, Yu Wang, Huandi Zhou, Xiaoying Xue","doi":"10.2147/IJGM.S521390","DOIUrl":"10.2147/IJGM.S521390","url":null,"abstract":"<p><strong>Objective: </strong>The goal of this study was to analyze in depth the importance of the thymosin beta 4 X-linked gene (TMSB4X) in the disease process of gliomas for the prediction of patient prognosis.</p><p><strong>Methods: </strong>We explored the expression of TMSB4X by analyzing datasets from The Cancer Genome Atlas (TCGA) datasets and the Chinese Glioma Genome Atlas (CGGA). Tumor sample tissues and corresponding paracancerous tissues of glioma patients were collected, and TMSB4X expression in glioma tissues was analyzed using Real-Time PCR to investigate its prognostic significance in glioma patients. In addition, we have plotted the ROC curves for the survival rate, performed univariate and multivariate Cox analyses and constructed nomogram. Differential expression analyses were performed on the basis of median expression levels of the TMSB4X gene, and Intuitive graphical presentation and visualisation of the 403 proteins screened were carried out using the Cytoscape software platform. (version: 3.9.1). The top 50 core genes were then screened using the cyto Hubba algorithm, these genes were analyzed using The Gene Ontology Database (GO) and the Kyoto Encyclopedia of Genes and Genomes (KEGG). TCGA datasets was utilized for Gene Set Enrichment Analysis (GSEA) to obtain potential NF-κB-related pathways in gliomas.</p><p><strong>Results: </strong>Independent prognostic analyses show that the TMSB4X gene was identified as an independent prognostic indicator for gliomas. In GOKEGG and GSEA analyses, the analyses indicate that TMSB4X may play a crucial role in glioma patients by up-regulating I-kappaB kinase/NF-kappaB signaling. In glioma patients, upregulation of the I-kappaB kinase and NF-kappaB signalling pathways plays a crucial role, which may be linked to CASP1.</p><p><strong>Conclusion: </strong>The findings showed that TMSB4X was identified as an potential independent risk factor in the prognosis of glioma patients, a finding that implies that TMSB4X has the potential to serve as a key biomarker for predicting the prognostic status of glioma patients.</p>","PeriodicalId":14131,"journal":{"name":"International Journal of General Medicine","volume":"18 ","pages":"2923-2940"},"PeriodicalIF":2.1,"publicationDate":"2025-06-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12152311/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144274831","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Exploring the Potential Regulatory Mechanisms of Mitophagy in Ischemic Cardiomyopathy.","authors":"Zhaobin Li, Jiajie Kong, Shuqiang Xi, Zeyue Jin, Fan Yang, Zhe Zhu, Lei Liu","doi":"10.2147/IJGM.S519388","DOIUrl":"10.2147/IJGM.S519388","url":null,"abstract":"<p><strong>Purpose: </strong>Ischemic cardiomyopathy (ICM) was a clinical syndrome. Long - term myocardial blood supply insufficiency, caused by coronary atherosclerotic plaque, led to myocardial nutritional disorders and atrophy. After large - scale myocardial infarction, fibrous tissue hyperplasia impaired cardiac systolic and/or diastolic functions, causing heart failure and arrhythmia. Study shows that dysregulated mitophagy can lead to cardiomyocyte death and cardiomyopathy. However, it is still uncertain how mitophagy related genes (MRGs) may affect the diagnosis of ICM.</p><p><strong>Patients and methods: </strong>Data were obtained from public databases. Subsequently, mitochondria autophagy score-related genes (MSRGs) were obtained through Weighted Gene Co-expression Network Analysis (WGCNA). Then, an intersection was taken between MSRGs and the differentially expressed genes (DEGs) obtained from the differential expression analysis to obtain DE-MSRGs. Then, biomarkers were identified through machine learning algorithms and Receiver Operating Characteristic curve (ROC) analysis. Next, analyses of immune infiltration, molecular regulatory network, and drug prediction were carried out. Finally, Reverse Transcription Quantitative Polymerase Chain Reaction (RT-qPCR) was performed on the biomarkers. It provides a certain theoretical basis for the research on the mechanism of the occurrence and development of ICM.</p><p><strong>Results: </strong>In total, 99 DE-MSRGs between ICM and control groups were gained. The four biomarkers (PPDPF, DPEP2, LTBP1, SOCS2) were acquired, and all biomarkers had good diagnostic efficacy for ICM. The content of 3 immune cells between ICM and control groups was significantly different, namely T cells, CD8+ T cells, and neutrophil, and all biomarkers were considerably positively correlated with T cells. The ceRNA network contained 4 mRNAs, 14 miRNAs, and 12 lncRNAs, and TF-mRNA network contained 32 nodes and 38 edges. Finally, 45 drugs targeting the biomarkers were predicted, such as Salmeterol, Histamine, Rotavirus vaccine, etc. Importantly, this all 4 biomarkers were higher in ICM samples in RT-qPCR analysis.</p><p><strong>Conclusion: </strong>Our findings provided four mitophagy related biomarkers (PPDPF, DPEP2, LTBP1, and SOCS2) for diagnosis of ICM, providing a scientific reference for further studies of ICM.</p>","PeriodicalId":14131,"journal":{"name":"International Journal of General Medicine","volume":"18 ","pages":"2881-2899"},"PeriodicalIF":2.1,"publicationDate":"2025-06-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12147806/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144258016","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Endoscopic Ultrasound-Guided Fine Needle Biopsy with Stylet and Suction Versus No Stylet No Suction for the Diagnosis of Pancreatic and Non-Pancreatic Lesions.","authors":"Marwa A Ahmed, Mohamed Yousri Ahmed, Hossam Eldin Shaaban, Sameh E A Abou Elenin, Ahmed Ali El-Habashi, Fatima Belabbes, Mohammed Tag-Adeen, Abeer Abdellatef, Hussein Hassan Okasha","doi":"10.2147/IJGM.S516107","DOIUrl":"10.2147/IJGM.S516107","url":null,"abstract":"<p><strong>Background: </strong>Endoscopic ultrasound (EUS)-guided tissue acquisition is the procedure of choice to obtain samples to diagnose pancreatic and non-pancreatic lesions. Previous randomized trials demonstrated that using stylets during the EUS-fine needle biopsy (FNB) technique does not improve the diagnostic yield. However, little data exists regarding the role of stylet and suction in the core biopsy needle during EUS-guided tissue acquisition.</p><p><strong>Aim of the study: </strong>This study aims to assess the efficacy and diagnostic yield of samples collected by EUS-FNB with stylet and suction versus no stylet, no suction techniques.</p><p><strong>Patients and methods: </strong>This prospective study included 167 patients referred to the Gastroenterology Division, Internal Medicine Department, Kasr Al-Aini Hospitals for EUS-guided biopsy from pancreatic or non-pancreatic lesions from May 2021 to January 2024. Each lesion was sampled twice using an EUS-FNB 22-gauge Franseen-tip needle (Acquire; Boston Scientific, USA); one sample was collected with stylet and suction, while the other with no stylet no suction technique. Subsequently, slides were evaluated for cellularity, tissue integrity, degree of blood contamination, and diagnostic ability.</p><p><strong>Results: </strong>A total of 167 patients fulfilled the inclusion criteria, 78 females and 89 males, with a mean age of 61 years. Most lesions were pancreatic (83.2%, n = 139), while the remaining (16.8%, n = 28) were non-pancreatic. No significant differences were observed between stylet and suction versus no stylet, no suction techniques. The diagnostic performance, including sensitivity, specificity, and overall accuracy, was identical (100%) for both methods when evaluating alcohol-fixed smears in conjunction with formalin-preserved tissues collected through FNB.</p><p><strong>Conclusion: </strong>EUS-FNB tissue acquisition with and without stylet and suction provides comparable diagnostic yield and tissue quality for pancreatic and non-pancreatic lesions. The decision to use a stylet and suction should be individualized based on the clinical context and operator experience.</p>","PeriodicalId":14131,"journal":{"name":"International Journal of General Medicine","volume":"18 ","pages":"2851-2860"},"PeriodicalIF":2.1,"publicationDate":"2025-06-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12145781/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144247787","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Development and Validation of an Anoikis-Related Gene Signature for Prognostic Prediction in Cervical Cancer.","authors":"Silu Meng, Xiangqin Li, Jianwei Zhang, Xiaodong Cheng","doi":"10.2147/IJGM.S508059","DOIUrl":"10.2147/IJGM.S508059","url":null,"abstract":"<p><strong>Background: </strong>Cervical cancer still has high incidence and mortality rates worldwide. This study aimed to evaluate the prognostic value of anoikis-related genes (ARGs) and develop a risk scoring model for accurate survival prediction in cervical cancer patients.</p><p><strong>Methods: </strong>The expression profiles of cervical cancer tissue and survival data were downloaded from TCGA-CESC and CGCI-HTMCP-CC. We identified 83 ARGs significantly associated with patients' survival. Subsequently, we developed a risk-scoring model based on 10 key genes. We assessed the predictive performance of our model by survival analysis, ROC curve analysis, and a nomogram that incorporated clinical factors. Additionally, we validated the expression of Granzyme B (GZMB) by immunohistochemical staining. Furthermore, we compared the biological processes and pathway enrichment in high-risk and low-risk patient groups, using differential gene expression and functional enrichment analysis. Finally, we investigated the immune microenvironment of patients in both high-risk and low-risk groups.</p><p><strong>Results: </strong>Patients in the high-risk group had significantly poorer survival compared to those in the low-risk group. The immunohistochemical results suggested that GZMB was associated with the prognosis of cervical cancer patients. The risk scoring model showed high accuracy in predicting the prognosis of cervical cancer patients. Differential gene expression analysis revealed enriched pathways related to tumor invasion and metastasis in the high-risk group. Conversely, the low-risk group showed a strong association with the activation of immune response pathways.</p><p><strong>Conclusion: </strong>This study concluded that anoikis-related genes played a crucial role in determining the prognosis of individuals with cervical cancer. This discovery not only presented potential biomarkers but also provided valuable insights for informing treatment strategies. The risk scoring model may assist clinicians in better identifying high-risk patients and personalizing treatment plans.</p>","PeriodicalId":14131,"journal":{"name":"International Journal of General Medicine","volume":"18 ","pages":"2861-2879"},"PeriodicalIF":2.1,"publicationDate":"2025-06-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12146097/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144258015","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The Impact of Aerobic Exercise on Health Management in Older Patients with Hypertension: A Systematic Review of Randomized Controlled Trials from the Past Decade.","authors":"Bingxue Zhang, Hongjuan Hu, Ziyi Mi, Haidi Liu","doi":"10.2147/IJGM.S516371","DOIUrl":"10.2147/IJGM.S516371","url":null,"abstract":"<p><strong>Purpose: </strong>This study was based on the PICO framework to systematically evaluate the effects of aerobic exercise on key health management indicators such as blood pressure, heart rate and cardiorespiratory fitness in older hypertensive patients.</p><p><strong>Patients and methods: </strong>A systematic search of randomized controlled trials from four English language databases, Web of Science, PubMed, Cochrane, and Embase, and four Chinese language databases, CNKI, VIP, Wanfang, and Sinomed, was performed (April 2014 to April 2024). StataCorp Stata v.18.0 was used for data analysis. In a random-effects meta-analysis, continuous variables were represented by the mean difference, and each effect size was represented by a 95% confidence interval.</p><p><strong>Results: </strong>Nine randomized controlled trials with 484 participants were included. The meta-analysis revealed that compared with the control group, participants engaging in aerobic exercise significantly reduced systolic blood pressure (SMD = -0.93, 95% CI = -1.48 to -0.39, <i>P</i> =0.001), diastolic blood pressure (SMD = -0.48, 95% CI = -0.75 to -0.21, <i>P</i> =0.001), and heart rate (SMD = -1.78, 95% CI = -3.31 to -0.24, <i>P</i> =0.024), and improved cardiorespiratory health (SMD =0.71, 95% CI =0.24 to 1.18, <i>P</i> =0.003).</p><p><strong>Conclusion: </strong>Older patients with hypertension aged 60 years should engage in 120-150 minutes of low- to moderate-intensity aerobic exercise per week, maintaining 40-75% maximum HR or 40-60% VO₂max (20-30 minutes per day, 5 days per week, or 75-150 minutes of exercise only once or twice a week. However, it is crucial that individuals assess their own health conditions, make appropriate time adjustments, and gradually increase the duration and intensity of exercise. And central randomization with blinded assessment should be used in future randomized controlled trials to reduce implementation bias and measurement bias.</p>","PeriodicalId":14131,"journal":{"name":"International Journal of General Medicine","volume":"18 ","pages":"2823-2838"},"PeriodicalIF":2.1,"publicationDate":"2025-06-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12145098/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144247789","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"KRT23 as a Potential Target for Metabolic Dysfunction-Associated Fatty Liver Disease (MAFLD): Evidence From Bioinformatics Analysis, Human Gene Polymorphism and Animal Experiments.","authors":"Yangmin Hao, Tao Zhang, Shaliyan Tuerxunmaimaiti, Ye Tian, Xinyu Wang, Zhiming Li, Liang Zhao, Lei Bai, Qu Chen, Cheng Li, Ayiguzhali Abulitipu, Rui Wang, Sheng Jiang, Guoli Du","doi":"10.2147/IJGM.S520326","DOIUrl":"10.2147/IJGM.S520326","url":null,"abstract":"<p><strong>Background: </strong>Metabolic dysfunction-associated fatty liver disease (MAFLD) is highly prevalent in Xinjiang, with genetic factors influencing its pathogenesis. Keratin 23 (KRT23), a liver-enriched protein linked to metabolic regulation, remains understudied in MAFLD genetics.</p><p><strong>Objective: </strong>To investigate associations between KRT23 gene polymorphisms, expression, and MAFLD in Xinjiang.</p><p><strong>Methods: </strong>This study enrolled 1,795 MAFLD patients diagnosed via ultrasonography and metabolic criteria. KRT23 polymorphisms (rs72826004, rs2269859) were analyzed. GEO database screening identified MAFLD-related genes. KRT23 expression was assessed in human serum/liver tissues (ELISA, Western blot, qRT‒PCR, IHC) and murine models (high-fat diet-induced MAFLD and db/db mice).</p><p><strong>Results: </strong>Enrichment analysis identified 10 key MAFLD-associated genes, including KRT23. The rs72826004 TT genotype increased MAFLD risk (OR: 2.156, P=0.007), while rs2269859 TT conferred protection (OR: 0.306, P=0.002). MAFLD patients exhibited elevated KRT23 protein/mRNA levels in serum and liver versus controls. Murine models confirmed higher KRT23 expression in MAFLD and db/db mice compared to wild-type.</p><p><strong>Conclusion: </strong>KRT23 gene polymorphism was associated with the occurrence of MAFLD. The rs72826004 loci TT genotype may be a risk factor for MAFLD, whereas the rs2269859 loci TT genotype may be a protective factor against MAFLD. Higher KRT23 expression (protein and mRNA) is related to MAFLD. KRT23 is a potential target for the treatment of MAFLD.</p>","PeriodicalId":14131,"journal":{"name":"International Journal of General Medicine","volume":"18 ","pages":"2807-2821"},"PeriodicalIF":2.1,"publicationDate":"2025-06-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12145148/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144247788","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Efficacy and Safety of Vedolizumab with Glucocorticoids vs Infliximabin Severe Ulcerative Colitis: A Retrospective Study.","authors":"Chenfei Zhang, Xiaoling Huang, Mailidan Maimaitiniyazi, Tingwei Zhou","doi":"10.2147/IJGM.S525638","DOIUrl":"10.2147/IJGM.S525638","url":null,"abstract":"<p><strong>Objective: </strong>To compare the efficacy and safety of vedolizumab plus glucocorticoids versus infliximab in severe ulcerative colitis for optimized treatment selection.</p><p><strong>Methods: </strong>Patients admitted to the Department of Gastroenterology of Xinjiang Uygur Autonomous Region People's Hospital with confirmed diagnosis of ulcerative colitis from January 2020 to December 2022 were collected, and according to the biologics used, the patients were divided into Vedolizumab combined with glucocorticoids group (n = 23) and infliximab group (n = 23). Clinically relevant indicators of the patients were retrospectively analyzed.</p><p><strong>Results: </strong>The difference between the basic information of the two groups of patients before treatment was not significant (<i>p</i> > 0.05). WBC 30 weeks, ESR (6 weeks, 30 weeks), ALB 30 weeks, Hb (6 weeks, 14 weeks, 30 weeks) of the difference between the groups was statistically significant (<i>p</i> < 0.05). WBC, CRP, ESR, ALB, Hb were tested by ANOVA, the differences between groups were statistically significant (<i>p</i> < 0.05). Comparison of the incidence of various complications between the two groups of patients, the difference was not significant (<i>p</i> > 0.05). The clinical response rate, clinical remission rate and mucosal healing rate of the 2 groups were statistically analyzed in terms of treatment effects at weeks 6, 14 and 30, respectively. Comparison between the groups showed that the differences in clinical response rate at week 6, clinical remission rate and endoscopic remission rate at week 30 and at weeks 14 and 30 were not statistically significant (<i>p</i> > 0.05); at weeks 14 and 30, the clinical response rate of group A was better than that of group B, and the differences were statistically significant (<i>p</i> < 0.05). At week 14, the clinical remission rate of group A was better than that of group B, and the difference was statistically significant (<i>p</i> < 0.05).</p><p><strong>Conclusion: </strong>Vedolizumab and infliximab are effective for severe UC, but vedolizumab with glucocorticoids outperforms infliximab in clinical response, remission, and long-term outcomes.</p>","PeriodicalId":14131,"journal":{"name":"International Journal of General Medicine","volume":"18 ","pages":"2839-2849"},"PeriodicalIF":2.1,"publicationDate":"2025-06-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12145104/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144247712","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Value of CT-Based 3D Reconstruction in Analyzing Fracture Line Distribution in Postmenopausal Women with Osteoporotic Pelvic Fractures.","authors":"Chunming Si, Baolin Bai, Wei Cong, Lipeng Zhang, Ruisheng Guan","doi":"10.2147/IJGM.S506333","DOIUrl":"10.2147/IJGM.S506333","url":null,"abstract":"<p><strong>Objective: </strong>This study aimed to evaluate the application value of CT-based 3D reconstruction in analyzing the distribution of pelvic fracture lines and to identify key factors, that affect fracture patterns in postmenopausal women with osteoporotic pelvic fractures.</p><p><strong>Methods: </strong>A total of 150 postmenopausal female patients with osteoporotic pelvic fractures who underwent CT scans in our radiology department from June 2022 to June 2023 were included. Subjects were divided into a normal group (n=60) and a pelvic fracture group (n=90). CT-based 3D reconstruction was used to analyze the distribution of fracture lines. The correlations between fracture lines and various factors were evaluated, such as age, bone mineral density (BMD) and body mass index (BMI).</p><p><strong>Results: </strong>The results showed a significant difference in BMD between the pelvic fracture group and the normal group. The average BMD in the pelvic fracture group was 0.763 ± 0.026 g/cm², which was significantly lower than 0.925 ± 0.051 g/cm² in the normal group (<i>P</i> < 0.001). This finding suggests that BMD plays an important role in the risk of pelvic fractures. 3D reconstruction revealed that fractures were more widespread in low BMD regions and fewer in high BMD regions, highlighting the correlation between lower BMD and higher fracture risk.</p><p><strong>Conclusion: </strong>CT-based 3D reconstruction enhances the assessment of pelvic fractures by providing a detailed evaluation of fracture line distribution. This study found that lower bone mineral density is a significant risk factor for pelvic fractures, with a direct correlation to the number and distribution of fracture lines.</p>","PeriodicalId":14131,"journal":{"name":"International Journal of General Medicine","volume":"18 ","pages":"2799-2806"},"PeriodicalIF":2.1,"publicationDate":"2025-05-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12135960/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144225414","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Identification and Validation of Key Genes Involved in the Coupling of Mitochondria-Associated Endoplasmic Reticulum Membrane in Hemorrhoidal Disease.","authors":"Lihua Mao, Zhiying Rao, Yanru Wang, Jun Yang, Junmei He, Zhi Zheng, Lanyu Chen","doi":"10.2147/IJGM.S511281","DOIUrl":"10.2147/IJGM.S511281","url":null,"abstract":"<p><strong>Background: </strong>Hemorrhoidal disease (HD) is the most prevalent rectal disorder, with various cellular processes influenced by the mitochondria-associated endoplasmic reticulum membrane (MAM). Potential therapeutic mechanisms for HD may be associated with MAM. This study aims to identify key genes linked to MAM in HD and to provide novel therapeutic targets.</p><p><strong>Methods: </strong>Transcriptome data and MAM-related genes (MAM-RGs) were obtained from the Gene Expression Omnibus (GEO) database and relevant literature. Differential expression analysis and single-sample Gene Set Enrichment Analysis (ssGSEA) scores were initially employed to identify candidate genes. Key genes were further refined using Least Absolute Shrinkage and Selection Operator (LASSO) and Protein-Protein Interaction (PPI) networks. A nomogram based on these key genes was developed and assessed. Additionally, CIBERSORT algorithms were utilized to evaluate immune cell infiltration abundance, differences, and correlations in the samples. Finally, the expression of key genes was validated via reverse transcription-quantitative PCR (RT-qPCR).</p><p><strong>Results: </strong>Differential expression analysis identified 956 differentially expressed genes (DEGs), and ssGSEA identified 143 differentially expressed MAM-RGs. A total of 50 candidate genes were selected through their intersection. Machine learning identified two key genes, <i>MUC16</i> and <i>DEFA5</i>. A nomogram with strong predictive capability was constructed. Immune cell analysis revealed two types of differential immune cells-activated dendritic cells and plasma cells-where activated dendritic cells were more highly expressed in the case group, and plasma cells showed a strong positive correlation with <i>DEFA5</i>. Additionally, <i>MUC16</i> was significantly overexpressed in patients with HD, while <i>DEFA5</i> exhibited down-regulation compared to controls.</p><p><strong>Conclusion: </strong>This study identifies <i>MUC16</i> and <i>DEFA5</i> as key genes associated with HD and MAM and presents a predictive nomogram with high accuracy. These findings provide novel insights into the mechanisms and potential treatment targets for HD.</p>","PeriodicalId":14131,"journal":{"name":"International Journal of General Medicine","volume":"18 ","pages":"2781-2798"},"PeriodicalIF":2.1,"publicationDate":"2025-05-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12136070/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144225413","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}