International Journal of General Medicine最新文献

{"title":"Comparing Different Contrast Injection Methods for Multislice Spiral CT Imaging in Triple-Rule-Out Examinations: A Study on Acute Chest Pain Patients.","authors":"Yue Meng, Fang Li, Yan Gu, Ying Zhou, Yonggang Zhang, Yongbao Liu, Zhongchang Miao","doi":"10.2147/IJGM.S496454","DOIUrl":"10.2147/IJGM.S496454","url":null,"abstract":"<p><strong>Introduction: </strong>The traditional injection method of contrast media (CM) has limitations in terms of volume and imaging quality. Choosing the optimal CM injection method is crucial for ensuring high-quality images and accurate diagnosis in triple-rule-out computed tomography angiography (TRO-CTA). This study aims to investigate the application value of combining region of interest (ROI) with CM injection method alterations in TRO-CTA.</p><p><strong>Methods: </strong>A total of 596 patients with chest pain, chest tightness, and dyspnea as the primary clinical symptoms, underwent TRO-CTA examinations based on their clinical symptoms and test indicators. These patients were randomly assigned to one of three groups: the traditional group, the modified group, and the high-pitch helical scan (FLASH) group. Key parameters, including computed tomography (CT) values, vascular filling contrast numbers, image noise, signal-to-noise ratio, contrast noise ratio, image scores, CM volume, CM injection rate, radiation dose of the aorta, pulmonary artery, and coronary artery, were systematically compared across the three groups.</p><p><strong>Results: </strong>The CM volume and radiation doses were significantly higher in the traditional group compared to the other groups (P < 0.05). CT values for the aorta and coronary artery in the traditional group were higher than those in the modified and FLASH group, while CT values for the pulmonary artery were lower in the traditional group compared to the modified and FLASH groups (ALL P-value for traditional group versus FLASH group, P-value for modified group versus traditional group < 0.05, P-value for modified group versus FLASH group > 0.05).</p><p><strong>Conclusion: </strong>The combination of the modified CM injection method and FLASH scanning mode reduces the required CM volume by nearly 30 mL and significantly decreases radiation dose exposure during the examination. This approach shows valuable potential for improving both the safety and effectiveness of TRO-CTA, with significant implications for clinical practice.</p>","PeriodicalId":14131,"journal":{"name":"International Journal of General Medicine","volume":"18 ","pages":"1231-1246"},"PeriodicalIF":2.1,"publicationDate":"2025-03-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11892740/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143597128","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Elderly Patients with Tuberculosis Combined with Diabetes Mellitus: A Comprehensive Analysis of Lymphocyte Subpopulation Dynamics, Clinical Features, Drug Resistance and Disease Regression.","authors":"Xin Wang, Yu Song, Na Li, JingYi Huo, BingBing Wang, Xue Jiang, YuLiang Zhang","doi":"10.2147/IJGM.S502996","DOIUrl":"10.2147/IJGM.S502996","url":null,"abstract":"<p><strong>Objective: </strong>The aim of this study was to evaluate lymphocyte subsets and clinical outcomes in patients with pulmonary tuberculosis (PTB) combined with type 2 diabetes mellitus (T2DM).</p><p><strong>Methods: </strong>Between January 2022 and March 2024, 320 patients aged > 60 years were included, 95 of whom had comorbid DM. Lymphocyte subsets (T-lymphocytes, B-lymphocytes, and natural killer cells, NK) were assessed in both groups of patients using fluorescence-associated cell sorting. Clinical characteristics, drug resistance, regression and lymphocyte subsets were compared in PTB patients with DM versus PTB patients alone. The correlation between lymphocyte subsets-related indices and the above characteristics in PTB patients with DM was analyzed.</p><p><strong>Results: </strong>Compared with PTB patients alone, PTB patients with comorbid DM had a higher positive rate (81.05% vs 62.67%, <i>P</i> = 0.001), prolonged course of antituberculosis treatment (298 [244, 339] days vs 223 [179, 254], <i>P</i> < 0.001) and a lower cured rate (64.21% vs 75.56%, <i>P</i> = 0.039). Compared with PTB patients only, PTB patients with comorbid DM had higher T-lymphocytes and CD4⁺ T-lymphocytes (<i>P</i> < 0.001, <i>P</i> = 0.006) and lower NK cells (<i>P</i> < 0.001). In PTB patients with comorbid DM, both treatment-prolonged patients and drug-resistant patients showed reduced NK cells. NK cells were slightly elevated in cured patients, although there was no significant difference (<i>P</i> = 0.082).</p><p><strong>Conclusion: </strong>Lymphocyte homeostasis is altered in PTB patients with comorbid DM, making them more susceptible to severe clinical manifestations and therapeutic outcomes. Notably, NK cells have been identified as key regulatory cells with a significant impact on prolonged treatment course and drug resistance. This study provides new ideas and methods for individualized treatment of PTB combined with DM in the elderly, which can help to optimize the treatment plan and improve the outcome and quality of life of patients.</p>","PeriodicalId":14131,"journal":{"name":"International Journal of General Medicine","volume":"18 ","pages":"1271-1282"},"PeriodicalIF":2.1,"publicationDate":"2025-03-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11892736/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143597133","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Inter- and Intra-Observer Reliability of the New Comprehensive Classification of Intertrochanteric Fracture of the Femur.","authors":"Xiaopei Xu, Yang Liu, Shuo Diao, Junlin Zhou","doi":"10.2147/IJGM.S508342","DOIUrl":"10.2147/IJGM.S508342","url":null,"abstract":"<p><strong>Objective: </strong>To evaluate the reliability of the new Comprehensive Classification in clinical practice by comparing it with Evans-Jensen (1975), AO/OTA (2007), and AO/OTA (2018).</p><p><strong>Methods: </strong>A total of 192 patients with intertrochanteric fractures were included in the study. The fractures were classified using the Comprehensive Classification system and the three traditional methods by eight observers, consisting of four high-experienced and four less-experienced surgeons. Interobserver and intraobserver agreements were assessed using kappa statistics and compared across the different classification systems.</p><p><strong>Results: </strong>The study included 192 patients. The average inter-observer Kappa values using CT scan for Evans-Jensen (1975), AO/OTA (2007), AO/OTA (2018), and Comprehensive Classification were 0.361 ± 0.06, 0.348 ± 0.05, 0.408 ± 0.06, and 0.492 ± 0.02, respectively. The average inter-observer Kappa values based on 3DR were 0.411 ± 0.04, 0.396 ± 0.03, 0.442 ± 0.03, and 0.524 ± 0.03, respectively. The average intra-observer Kappa values using CT scan were 0.467 ± 0.05, 0.453 ± 0.06, 0.542 ± 0.04, and 0.631 ± 0.02, respectively. The average intra-observer Kappa values using 3DR were 0.503 ± 0.05, 0.482 ± 0.03, 0.561 ± 0.03, and 0.672 ± 0.02, respectively. The mean kappa value of Inter- and intra-observer for the Comprehensive Classification were higher than Evans-Jensen (1975) and AO/OTA (2007/2018) using CT scans and 3DR (P < 0.05). Additionally, the high-experienced group using 3DR had the highest agreement, at approximately 75.86%, when comparing the implant type corresponding to the fracture classification evaluated by the observer with the implant type selected by the observer.</p><p><strong>Conclusion: </strong>The Comprehensive Classification system emerges as a reliable and superior tool for the classification of intertrochanteric fractures, offering improved agreement rates and practical treatment insights compared to traditional methods. This classification has guiding significance for treatment.</p>","PeriodicalId":14131,"journal":{"name":"International Journal of General Medicine","volume":"18 ","pages":"1261-1270"},"PeriodicalIF":2.1,"publicationDate":"2025-03-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11892744/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143597096","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Neutrophil Extracellular Traps Enhance Procoagulant Activity and Predict Poor Prognosis in Patients With Metastatic Breast Cancer.","authors":"Youwei Gong, Binjie Chen, Qixing Tan, Wei Wei","doi":"10.2147/IJGM.S511024","DOIUrl":"10.2147/IJGM.S511024","url":null,"abstract":"<p><strong>Objective: </strong>Neutrophil extracellular traps (NETs) are associated with poor prognosis and an increased risk of venous thromboembolism (VTE) in metastatic breast cancer (MBC). This study aims to determine whether NETs promote hypercoagulability and if NETs and plasma hypercoagulability markers are biomarkers of survival in MBC.</p><p><strong>Methods: </strong>Circulating levels of neutrophil extracellular trap (NET) markers and hypercoagulability markers (TAT, fibrinogen, and D-dimer) were assessed in 112 MBC patients before treatment, compared to 55 healthy controls. Stratified by NET levels and plasma TAT, fibrinogen, and D-dimer, the correlation with overall survival was analyzed. The NET procoagulant activity was evaluated using fibrin and purified coagulation complex production assays, and by measuring coagulation time (CT).</p><p><strong>Results: </strong>MBC patients exhibited significantly elevated plasma NET levels compared to healthy controls (all <i>P</i><0.05), circulating MPO-DNA and NE-DNA levels were positively correlated with plasma TAT, fibrinogen, D-dimer, CT, FVIIIa, and platelet (PLT) counts. Additionally, we observed a significant increase in NETs formation in control neutrophils exposed to MBC plasma compared to those exposed to control plasma. NETs from MBC neutrophils significantly increased the potency of control plasma to generate thrombin and fibrin, effects that were notably attenuated by DNase I. Plasma TAT and D-dimer levels were significantly higher in MBC patients who died within three years post-recruitment compared to those who survived beyond three year. Plasma TAT and D-dimer were inversely correlated with survival. High plasma levels of MPO-DNA were associated with significantly worse overall survival (HR: 2.445, 95% CI: 1.255-4.762, <i>P</i>=0.007). MBC patients with both high D-dimer and high MPO-DNA had significantly reduced survival (HR: 2.450, 95% CI: 1.332-4.488, <i>P</i>=0.002).</p><p><strong>Conclusion: </strong>Our results highlight the increased release of NETs in MBC patients and reveal that NET formation enhances hypercoagulability and cancer progression. Targeting NETs may be a potential therapeutic strategy to inhibit MBC progression and mitigate thrombotic complications in MBC.</p>","PeriodicalId":14131,"journal":{"name":"International Journal of General Medicine","volume":"18 ","pages":"1247-1259"},"PeriodicalIF":2.1,"publicationDate":"2025-03-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11890004/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143585616","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A Questionnaire Study of Leadership in General Medicine: General Physicians in Japan are Facing Challenges in Education and Research [Response to Letter].","authors":"Risa Hirata, Masaki Tago, Hiromizu Takahashi, Takashi Watari, Kiyoshi Shikino, Yosuke Sasaki, Taro Shimizu","doi":"10.2147/IJGM.S522557","DOIUrl":"10.2147/IJGM.S522557","url":null,"abstract":"","PeriodicalId":14131,"journal":{"name":"International Journal of General Medicine","volume":"18 ","pages":"1215-1216"},"PeriodicalIF":2.1,"publicationDate":"2025-03-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11884409/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143573027","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Correlation Of Fut2 And Fut3 Gene Polymorphisms With Inflammatory Bowel Disease In Guangxi Zhuang Population.","authors":"Xiaodan Lv, Zhou Huang, Shiquan Li, Xiaofang Xu, Deyi Chen, Lichun Han, Yu He, Shan Huang, Huiling Wu, Yunwei Qi, Liqiang Wei, Min Chen, Lingling Zhan, Xiaoping Lv","doi":"10.2147/IJGM.S505711","DOIUrl":"10.2147/IJGM.S505711","url":null,"abstract":"<p><strong>Objective: </strong>This study aimed to investigate the association between the polymorphisms of the FUT2 gene (rs1047781, rs601338) and the FUT3 gene (rs3745635, rs28362459) with susceptibility to inflammatory bowel disease (IBD) in the Zhuang population of Guangxi.</p><p><strong>Methods: </strong>Intestinal mucosal tissues were collected from 113 Zhuang patients (41 with Crohn's disease [CD] and 72 with ulcerative colitis [UC]) and 120 han patients (42 with CD and 78 with UC), all of whom were unrelated and diagnosed with IBD, as well as from 106 Zhuang and 119 han unrelated healthy controls, from January 2021 to December 2023. DNA was extracted from intestinal mucosal samples in each group. FUT2 gene polymorphisms (rs1047781, rs601338) and FUT3 gene polymorphisms (rs3745635, rs28362459) were amplified using polymerase chain reaction (PCR). PCR product fragments were analyzed by DNA sequencing, and sequence analysis was conducted using the GenBank database.</p><p><strong>Results: </strong>The genotype and allele frequencies of the FUT2 rs1047781 polymorphism in the Zhuang UC patient group were significantly different from those in the control group (P < 0.05). Similarly, significant differences were observed in the genotype and allele frequencies of the FUT3 rs3745635 polymorphism in the Zhuang UC and CD patient groups compared to controls (P < 0.05). No statistically significant differences were found in the genotype and allele frequencies of FUT2 rs1047781 between the Zhuang CD patients and controls (P > 0.05). Additionally, no significant differences were observed in the genotype and allele frequencies of FUT2 rs601338 and FUT3 rs28362459 polymorphisms between the Zhuang UC and CD patient groups and the controls (P > 0.05).</p><p><strong>Conclusion: </strong>The FUT2 rs1047781 and FUT3 rs3745635 polymorphisms may be associated with IBD in the Guangxi Zhuang population, while FUT2 rs601338 and FUT3 rs28362459 polymorphisms may not show such an association.</p>","PeriodicalId":14131,"journal":{"name":"International Journal of General Medicine","volume":"18 ","pages":"1217-1230"},"PeriodicalIF":2.1,"publicationDate":"2025-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11881759/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143567023","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Practical Applications of Artificial Intelligence Diagnostic Systems in Fundus Retinal Disease Screening.","authors":"Qingquan Wei, Lifang Chi, Meiling Li, Qinghua Qiu, Qing Liu","doi":"10.2147/IJGM.S507100","DOIUrl":"10.2147/IJGM.S507100","url":null,"abstract":"<p><strong>Purpose: </strong>This study aims to evaluate the performance of a deep learning-based artificial intelligence (AI) diagnostic system in the analysis of retinal diseases, assessing its consistency with expert diagnoses and its overall utility in screening applications.</p><p><strong>Methods: </strong>A total of 3076 patients attending our hospital underwent comprehensive ophthalmic examinations. Initial assessments were performed using the AI, the Comprehensive AI Retinal Expert (CARE) system, followed by thorough manual reviews to establish final diagnoses. A comparative analysis was conducted between the AI-generated results and the evaluations by senior ophthalmologists to assess the diagnostic reliability and feasibility of the AI system in the context of ophthalmic screening.</p><p><strong>Results: </strong>: The AI diagnostic system demonstrated a sensitivity of 94.12% and specificity of 98.60% for diabetic retinopathy (DR); 89.50% sensitivity and 98.33% specificity for age-related macular degeneration (AMD); 91.55% sensitivity and 97.40% specificity for suspected glaucoma; 90.77% sensitivity and 99.10% specificity for pathological myopia; 81.58% sensitivity and 99.49% specificity for retinal vein occlusion (RVO); 88.64% sensitivity and 99.18% specificity for retinal detachment; 83.33% sensitivity and 99.80% specificity for macular hole; 82.26% sensitivity and 99.23% specificity for epiretinal membrane; 94.55% sensitivity and 97.82% specificity for hypertensive retinopathy; 83.33% sensitivity and 99.74% specificity for myelinated fibers; and 75.00% sensitivity and 99.95% specificity for retinitis pigmentosa. Additionally, the system exhibited notable performance in screening for other prevalent conditions, including DR, suspected glaucoma, suspected glaucoma, pathological myopia, and hypertensive retinopathy.</p><p><strong>Conclusions: </strong>: The AI-assisted screening system exhibits high sensitivity and specificity for a majority of retinal diseases, suggesting its potential as a valuable tool for screening practices. Its implementation is particularly beneficial for grassroots and community healthcare settings, facilitating initial diagnostic efforts and enhancing the efficacy of tiered ophthalmic care, with important implications for broader clinical adoption.</p>","PeriodicalId":14131,"journal":{"name":"International Journal of General Medicine","volume":"18 ","pages":"1173-1180"},"PeriodicalIF":2.1,"publicationDate":"2025-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11882464/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143573029","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Dynamic Immune Indicator Changes as Predictors of ARDS in ICU Patients with Sepsis: A Retrospective Study.","authors":"Xiaochi Lu, Yi Chen, Gongping Zhang, Xu Zeng, Linjie Lai, Chaojun Qu","doi":"10.2147/IJGM.S501252","DOIUrl":"10.2147/IJGM.S501252","url":null,"abstract":"<p><strong>Background: </strong>Understanding the dynamic changes in immune indicators during sepsis and their predictive value for Acute respiratory distress syndrome (ARDS) is crucial for improving patient outcomes.</p><p><strong>Methods: </strong>This single-center, observational retrospective study was conducted at Lishui Central Hospital, Zhejiang Province. Patients diagnosed with Sepsis-3 were categorized into non-ARDS and ARDS groups based on ARDS development. Data collection included demographics, clinical data, and immune parameters. Immune parameters were collected on days 1, 3, and 7 post-admission. Multivariate logistic regression analysis identified independent risk factors for ARDS, and a nomogram model was constructed. The predictive ability of the model was evaluated using ROC curves.</p><p><strong>Results: </strong>Multivariate analysis identified key factors for the nomogram, including CD4, CD8, Treg, lymphocyte, IgG, and IgA levels on Days 3 and 7. On Day 3, CD8 (P < 0.001), Tregs (P = 0.021), IgG (P < 0.001), and IgA (P < 0.001) showed significant negative correlations with ARDS development. On Day 7, CD4 (P < 0.001), CD8 (P < 0.001), lymphocyte count (P < 0.001), and IgA (P < 0.001) similarly demonstrated significant negative correlations with ARDS risk. The nomogram model had an AUC of 0.998 (95% CI: 0.997-0.999), indicating high predictive ability.</p><p><strong>Conclusion: </strong>Early dynamic changes in immune indicators, including CD8, CD4, Treg, IgA, IgG, and Lymphocyte, predict ARDS development in ICU sepsis patients.</p>","PeriodicalId":14131,"journal":{"name":"International Journal of General Medicine","volume":"18 ","pages":"1163-1172"},"PeriodicalIF":2.1,"publicationDate":"2025-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11882469/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143573028","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Correlation of Environmental Aerosols and Changes in Pulmonary Function of Pigeon Breeders in Turpan.","authors":"Mailidan Maimaitiniyazi, Xiaohong Yang","doi":"10.2147/IJGM.S508539","DOIUrl":"10.2147/IJGM.S508539","url":null,"abstract":"<p><strong>Background: </strong>To investigate the effects of aerosol size and concentration in pigeon lofts on the lung function of pigeon feeders.</p><p><strong>Methods: </strong>From January 2022 to February 2022, 135 pigeon-feeders randomly selected from four villages in Turpan City, Xinjiang Uygur Autonomous Region were included in the exposure group, and 135 healthy medical check-ups from the same villages who had not been exposed to pigeons were included in the control group, and were questioned in detail to record the relevant information about the study subjects, to complete the pulmonary function examination, and to examine the concentration and size of aerosols in the surroundings, to analyze the effects of different concentrations and sizes of pigeon house aerosols on the lung function of the pigeon-feeders. The effects of different concentrations and sizes of pigeon loft aerosols on the lung function (FEV1, FVC, FEV1%, FVC%, FEV1/FVC) of the pigeon breeders were analyzed, and the correlation between the lung function and the number of years of keeping pigeons, the number of pigeons kept, and the time of daily exposure were analyzed.</p><p><strong>Results: </strong>The aerosol concentration in the pigeon lofts of the exposed group was higher than that of the control group. Comparing the lung function of the exposed group with that of the control group, it was found that the lung function indexes of FVC, FEV1% and FVC% were lower than those of the control group, the aerosol concentration was negatively correlated with the lung function indexes of FEV1, FVC, FEV1%, FVC%, FEV1/FVC, the daily contact time with pigeons was negatively correlated with lung function indices, the number of years of keeping pigeons was negatively correlated with FEV1, and the difference was statistically significant.</p><p><strong>Conclusion: </strong>The higher the aerosol concentration in the pigeon lofts, the longer the years of keeping, the longer the daily contact time with pigeons, the more pronounced is the decrease of the lung function indices in pigeon feeders.</p>","PeriodicalId":14131,"journal":{"name":"International Journal of General Medicine","volume":"18 ","pages":"1193-1201"},"PeriodicalIF":2.1,"publicationDate":"2025-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11881754/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143567020","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"miR-145-5p Inhibits HER2-Positive Breast Cancer Cells via Targeting ARF6.","authors":"Lixian Yang, Wei Dang, Fanting Kong, Xuan Zhang, Lei Zheng, Lining Jia, Xiaowei Li, Na Lu","doi":"10.2147/IJGM.S510358","DOIUrl":"10.2147/IJGM.S510358","url":null,"abstract":"<p><strong>Background: </strong>The objective of this study was to examine how miR-145-5p contributes to inhibiting the growth and movement of breast cancer cells by targeting and modulating the ADP-ribosylation factor 6 gene, as well as to clarify the mechanisms involved.</p><p><strong>Methods: </strong>Bioinformatics analysis was used to study miR-145-5p expression in breast cancer samples from the TCGA database. RT-qPCR was performed on 25 pairs of HER2-positive breast cancer tissues and adjacent normal tissues, as well as in SK-BR3 and MCF10A cell lines. The effects of miR-145-5p overexpression on cell viability, migration, and invasion were assessed using CCK-8, scratch, and Transwell assays in SK-BR3 cells. A dual luciferase reporter assay was used to confirm miR-145-5p binding to the 3'-UTR of ARF6 mRNA. Additionally, the combined effects of miR-145-5p and ARF6 overexpression on SK-BR3 cell proliferation, migration, and invasion were evaluated.</p><p><strong>Results: </strong>The examination of the TCGA database indicated that the expression levels of miR-145-5p were reduced in both paired and unpaired breast cancer tissues in comparison to normal control breast tissues. Notably, miR-145-5p showed a remarkably lower expression in HER2-positive breast cancer tissues versus paraneoplastic tissues. When the cells were transfected with a miR-145-5p mimic, there was a significant reduction in SK-BR3 cell proliferation, migration, and invasion in vitro. Conversely, the transfection of the cells with a miR-145-5p inhibitor led to a notable increase in SK-BR3 cell proliferation, migration, and invasion. Furthermore, miR-145-5p was found to suppress the expression of ARF6 mRNA by directly interacting with its 3'-untranslated region.</p><p><strong>Conclusion: </strong>Overall, this study reveals that miR-145-5p suppresses the proliferation, migration, and invasion of breast cancer cells by interacting with ARF6 mRNA. Consequently, this miRNA might serve as a new target for accurate diagnosis and treatment of breast cancer.</p>","PeriodicalId":14131,"journal":{"name":"International Journal of General Medicine","volume":"18 ","pages":"1181-1192"},"PeriodicalIF":2.1,"publicationDate":"2025-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11881773/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143567026","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}