International Journal of General Medicine最新文献

{"title":"Clinical Efficacy of Qingke Mixture in Treating Mycoplasma Pneumonia in Children: A Randomized Controlled Trial.","authors":"Xiaofei Xie, Wei Wang, ShengTao Cui, Tianlong Xie, Yongfu Song, Na Wang, Zhuang Wang, Yongji Wang","doi":"10.2147/IJGM.S531095","DOIUrl":"10.2147/IJGM.S531095","url":null,"abstract":"<p><strong>Background: </strong>Mycoplasma pneumoniae pneumonia (MPP) is a common respiratory infection in children, current treatments are limited by resistance and side effects. This study aims to evaluate the clinical efficacy and safety of combining Qingke Mixture with azithromycin for treating MPP in children.</p><p><strong>Methods: </strong>This prospective, randomized, double-blind, controlled trial included 92 children diagnosed with MPP. The treatment group received Qingke Mixture and azithromycin, while the control group received azithromycin alone. Outcomes assessed included clinical symptoms, inflammatory markers, immune response, pulmonary function, and adverse events.</p><p><strong>Results: </strong>The treatment group showed significantly higher clinical efficacy (95.65% vs 80.43%, P < 0.05). The treatment group showed faster resolution of pulmonary symptoms, shorter hospital stays, and greater improvements in pulmonary function and TCM symptom scores (P < 0.05). Inflammatory markers (WBC, CRP, IL-6, IL-8) were significantly lower, while immunoglobulin levels (IgA, IgG, IgM) and T lymphocyte subsets (CD3+, CD4+/CD8+ ratio) were higher in the treatment group compared to the control group (P < 0.05). The incidence of adverse events was low in both groups, with no statistically significant difference.</p><p><strong>Conclusion: </strong>Qingke Mixture combined with azithromycin significantly improves clinical outcomes in children with MPP, enhancing efficacy while maintaining safety.</p>","PeriodicalId":14131,"journal":{"name":"International Journal of General Medicine","volume":"18 ","pages":"5073-5083"},"PeriodicalIF":2.0,"publicationDate":"2025-09-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12415112/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145029772","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"RCN1 Binds KIF14 and Promotes the Malignant Growth of Cervical Cancer Through the PI3K-AKT Pathway.","authors":"Yanyu Li, Li Cai, Jiayun Zhou, Xuping Zhang, Yunuo Zheng, Jingbo Zhang, Hui Cheng, Qing Wang, Bei Zhang","doi":"10.2147/IJGM.S531003","DOIUrl":"10.2147/IJGM.S531003","url":null,"abstract":"<p><strong>Purpose: </strong>The fourth most common cause of cancer-related deaths in women is cervical cancer. Though treatment of early-stage cervical cancer is often effective, middle and advanced stage cervical cancer is hard to treat and prone to recurrence. We sought to explore the mechanism underlying cervical cancer progression to identify new therapeutic approaches.</p><p><strong>Methods: </strong>Label-free mass spectrometry (LC-MS/MS) was used to identify differentially expressed proteins in cervical cancer and normal tissues. The findings were confirmed by Western blotting, RT-qPCR, and immunohistochemistry. The function of RCN1 in tumor invasion, metastasis, and proliferation was investigated using in vitro and in vivo tests. Immunoprecipitation tandem mass spectrometry (IP-MS) was performed in RCNI knockdown cells to identify downstream pathways. Western blotting was used for detecting the expressions of the key proteins included in KIF14 and PI3K-AKT-mTOR signaling pathways before and after RCN1 knockout.</p><p><strong>Results: </strong>RCN1 expression is elevated in patients with lymph node metastases and recurrent cervical cancer and correlates with poor prognosis. Knockdown and overexpression assays revealed that RCN1 promotes proliferation, migration and invasion of cervical cancer cells. RCN1 overexpression encourages metastasis in a mouse xenograft model. Furthermore, RCN1 targets KIF14, an activator of AKT, thus providing a molecular basis that could explain the malignant behavior of RCN1-expressing cervical cancer.</p><p><strong>Conclusion: </strong>RCN1 is significantly expressed in cervical cancer, which is associated with a poor prognosis, spread and recurrence. By promoting KIF14-induced activation of the PI3K-AKT-mTOR pathway, RCN1 may facilitate the malignant development of cervical cancer.</p>","PeriodicalId":14131,"journal":{"name":"International Journal of General Medicine","volume":"18 ","pages":"5047-5062"},"PeriodicalIF":2.0,"publicationDate":"2025-09-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12415097/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145029408","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Associations Between Inflammatory and Catecholamine Markers and Clinical Outcomes in People with Post-Acute SARS-CoV-2 Infection.","authors":"Lynn H Gerber, Micahel Estep, Leyla De Avila, Jillian K Price, Ali A Weinstein, Maria Stepanova, Aybike Birerdinc, Zobair Younossi","doi":"10.2147/IJGM.S534539","DOIUrl":"10.2147/IJGM.S534539","url":null,"abstract":"<p><strong>Purpose: </strong>The diagnosis of post-acute SARS-CoV-2 infection (PASC) is broad, referring to new or persistent health problems >four weeks after being infected with SARSCoV-2. The aim of this study was to determine whether cytokines, chemokines or catecholamine levels could specify the clinical condition.</p><p><strong>Patients and methods: </strong>Seventy-nine participants participated in person to study PASC. They were average 51 years (mean), 52% female, 62% Caucasian, 11% African American and 37% Hispanic with a mean BMI of 30.5. Most prevalent symptoms were fatigue, memory loss and shortness of breath. We extracted co-morbid conditions, length of hospital stay and course and laboratory values; medications, history of regular exercise (total of 150 minutes/week), measures of cognition (PCCOG), including Color Word Interference Test (CWIT), Coding, Arithmetic, Matrix Reasoning), clinical assessment of health behavior change, and several patient reported outcomes (PROs) (Edmonton Symptom Assessment System (ESAS), health-related quality of life instrument (EQ5D), anxiety and depression (GAD7, PHQ9) and fatigue (Functional Assessment of Chronic Illness Therapy - Fatigue (FACIT-F).</p><p><strong>Results: </strong>These data suggest that people with PASC are more likely to report lower levels of physical well-being, emotional well-being and higher fatigue levels than the non-PASC population. Epinephrine levels correlate statistically significantly with PROs (p<0.05), for overall FACIT-F, as well as the physical and functional subscales. The fatigue severity self-report, PHQ9 and number of symptoms were also significantly correlated. Interleukin-1 beta (IL1b) was inversely correlated with the Physical Well Being (PWB) and Emotional Well Being (EWB) FACIT-F subscales, the GAD7 and the PCCOG scale (p<0.05).</p><p><strong>Conclusion: </strong>Participants in this observational study of PASC report lower levels of emotional, physical well-being, more fatigue, anxiety and depression than are reported in population norms. Epinephrine and IL1b correlate with these findings and may offer a biological measurement, providing clinically useful information for tracking persistence or recovery. These findings may encourage further study to develop newer treatment approaches.</p>","PeriodicalId":14131,"journal":{"name":"International Journal of General Medicine","volume":"18 ","pages":"4975-4985"},"PeriodicalIF":2.0,"publicationDate":"2025-09-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12413844/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145015141","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Evaluation of Serum Levels of Kidney Injury Molecule-1 (KIM-1) and Neutrophil Gelatinase-Associated Lipocalin (NGAL) as Potential Biomarkers of Renal Tubular Damage in Brucellosis Patients.","authors":"Ahmet Dundar, Halit Irmak, Tugba Ayhanci, Songul Cetik Yildiz","doi":"10.2147/IJGM.S544766","DOIUrl":"10.2147/IJGM.S544766","url":null,"abstract":"<p><strong>Background: </strong>Brucellosis is a zoonotic and multisystemic disease that is widespread worldwide and can present with many different clinical conditions, ranging from asymptomatic to serious and fatal conditions. Brucellosis may be linked to renal tubular and acute kidney damage, nephrotic syndrome, and various types of nephropathies.</p><p><strong>Objective: </strong>Our research was carried out prospectively to investigate the relationship between brucellosis and various biochemical markers and particularly to investigate the role of renal tubular damage biomarkers.</p><p><strong>Methods: </strong>Demographic and biochemical data from 60 controls and 60 brucellosis patients were included in the study. Kidney injury molecule-1 (KIM-1) and Neutrophil gelatinase-associated lipocalin (NGAL) were analyzed by. The diagnostic accuracy of KIM-1 and NGAL for brucellosis was assessed using receiver operating characteristic (ROC) curve analysis.</p><p><strong>Results: </strong>According to our findings, significant differences of KIM-1 and NGAL were observed between brucellosis and healthy patients. A high AUC (area under the curve) value of 0.742 (95% CI: 0.616-0.868) and 0.835 (95% CI: 0.729-0.941) was observed for KIM-1 and NGAL, respectively.</p><p><strong>Conclusion: </strong>Serum levels of KIM-1 and NGAL, which are renal tubular damage markers, where higher in Brucellosis than in healthy patients. These biomarkers can contribute to the rapid and accurate diagnosis of brucellosis regarding the involvement of nephropathies and to the standardization of comprehensive diagnostic warning indicators.</p>","PeriodicalId":14131,"journal":{"name":"International Journal of General Medicine","volume":"18 ","pages":"5039-5046"},"PeriodicalIF":2.0,"publicationDate":"2025-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12413496/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145015164","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The Role of Sirtuins in Bone Repair From the Perspective of Glucose Metabolism.","authors":"Yun Ding, Yu Gao, Jianjun Sun, Zhigang Cai","doi":"10.2147/IJGM.S530523","DOIUrl":"10.2147/IJGM.S530523","url":null,"abstract":"<p><p>Impaired clinical fracture healing remains a major challenge, with surgical treatment often insufficient in patients with metabolic disorders or comorbidities such as diabetes and osteoporosis. Recent advances in metabolomics have brought the Sirtuin protein family to the forefront of bone regeneration research. These NAD⁺-dependent deacetylases exhibit cell-specific expression and regulate critical processes in osteoblasts and osteoclasts, linking glucose metabolism with bone remodeling. Sirtuins influence key pathways such as Wnt/β-catenin, AMPK, and mTOR, offering novel insights into the mechanisms of fracture healing. Emerging pharmacological strategies targeting Sirtuins show promising results in preclinical models. This review highlights the potential of Sirtuin-based interventions as therapeutic targets in the metabolic regulation of bone repair.</p>","PeriodicalId":14131,"journal":{"name":"International Journal of General Medicine","volume":"18 ","pages":"5013-5031"},"PeriodicalIF":2.0,"publicationDate":"2025-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12412762/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145015135","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The Imaging Evaluation of Left Atrial Appendage: CT Large-Spiral Arterial Late Scan.","authors":"Pan An, Qiang Tian, Shijun Duan, Xiulong Feng, Yu Han, Yaoning Wei, Wen Wang, Qian Yin","doi":"10.2147/IJGM.S537696","DOIUrl":"10.2147/IJGM.S537696","url":null,"abstract":"<p><strong>Purpose: </strong>Compared with retrospective ECG-gated arterial phase scan, to investigate the clinical application value of dual-source CT large-spiral arterial late scan in the imaging evaluation of left atrial appendage (LAA).</p><p><strong>Patients and methods: </strong>A total of 108 patients requiring LAA CT angiography (CTA) due to atrial fibrillation (AF) were selected from September 2024 to December 2024, including 52 patients in group A (Flash large-spiral arterial late scan) and 56 patients in group B (retrospective ECG-gated arterial phase scan). All patients underwent double-phase scan. The interval between the two periods is 30s. Clinical data of patients were collected, the scanning range and radiation dose received were recorded. The CT values of LAA, ascending aorta (AA) at the same level and left atrium at the largest level were measured. At the same time, evaluate other meaningful lesions within the scan range.</p><p><strong>Results: </strong>The difference between the two groups of scanning range and radiation dose was statistically significant (<i>P</i> < 0.05). There was statistical significance in the subjective evaluation and objective evaluation of LAA filling at the first phase scanning of the two groups (<i>P</i> < 0.05). The detection of other meaningful lesions in the scanning range of group A was significantly higher than that of group B.</p><p><strong>Conclusion: </strong>By adopting the three-generation dual-source FLASH large-pitch arterial late scan mode, the complete filling rate of the LAA was significantly improved. Not only shortened the examination time, reduced the radiation dose, but also increased the detection rate of other significant lesions within the scanning range for the patients.</p>","PeriodicalId":14131,"journal":{"name":"International Journal of General Medicine","volume":"18 ","pages":"4965-4973"},"PeriodicalIF":2.0,"publicationDate":"2025-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12412602/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145015203","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A Classification Method for General Medicine Physicians to Advance Field Research in Japan.","authors":"Masaki Tago, Risa Hirata, Kiyoshi Shikino, Takashi Watari, Hiromizu Takahashi, Tomoyo Nishi, Yosuke Sasaki, Taro Shimizu","doi":"10.2147/IJGM.S540846","DOIUrl":"10.2147/IJGM.S540846","url":null,"abstract":"<p><strong>Purpose: </strong>General medicine physicians in Japan play diverse roles depending on their work environments; however, there is no clear definition. In the United States, the establishment of a definition for \"hospitalists\" has contributed to the accumulation of evidence. To develop research in general medicine and build evidence in Japan, there is an urgent need to establish a systematic classification method for general medicine physicians.</p><p><strong>Methods: </strong>Based on discussions with six hospital-based general medicine physicians with over 10 years of experience at an academic conference in 2024 and a literature review, we proposed a classification method for general medicine physicians.</p><p><strong>Results: </strong>The proposed classification method was based on four indicators: physician maldistribution index, inpatient care contribution index (the contribution of one general medicine physician to inpatient care across all beds in the hospital), clinical content (home medical care, outpatient care, emergency response, inpatients management, and critical inpatients management), and education (medical students, clinical residents, and senior residents). These indicators were established by considering physician characteristics, target population, social environment, and hospital environment, enabling an objective evaluation of factors contributing to the practice of general medicine physicians.</p><p><strong>Conclusion: </strong>This classification method may serve as a foundation for research that makes it easier to understand the characteristics of general medicine physicians under study and allows for the comparison of roles according to regional characteristics and hospital size. Future validation and continuous improvement of this classification method are expected to advance the accumulation of evidence related to general medicine physicians. In this paper, we present a proposal for a classification method; future validation of this classification is necessary.</p>","PeriodicalId":14131,"journal":{"name":"International Journal of General Medicine","volume":"18 ","pages":"5033-5038"},"PeriodicalIF":2.0,"publicationDate":"2025-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12413545/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145015138","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"To Evaluate the Effect of Pediatric Massage Combined with Comprehensive Management on Growth Parameters and Biochemical Indicators in Children with Short Stature.","authors":"Qing Yang, Jing Wu, Xue Deng, Linrui Chen, Yanhui Chen","doi":"10.2147/IJGM.S535676","DOIUrl":"10.2147/IJGM.S535676","url":null,"abstract":"<p><strong>Purpose: </strong>To investigated the effect of pediatric massage combined with comprehensive management on height improvement in children with short stature.</p><p><strong>Patients and methods: </strong>This retrospective analysis included medical records of 80 children diagnosed with short stature, who received treatment at Wuhu Hospital of Traditional Chinese Medicine between April 2022 and April 2023. According to the recorded treatment modalities, patients were divided into two groups: the control group received standard comprehensive management (including psychological health guidance, diet, sleep, exercise), while the massage group received additional pediatric syndrome-based massage therapy alongside comprehensive treatment. Relevant clinical indicators-including height, weight, BMI (body mass index), IGF-1 (insulin-like growth factor-1), BA (bone age), 25(OH)D (25-hydroxyvitamin D), annual growth velocity (GV), TCM (Traditional Chinese Medicine) symptom scores, as well as cortisol, luteinizing hormone (LH), and growth hormone (GH) levels-were extracted from the medical records and compared between the two groups at baseline and after the recorded treatment period.</p><p><strong>Results: </strong>When comparing both groups of patients before and after the intervention, significant improvements were observed in height, weight, IGF-1, bone age index (BAI), 25(OH)D levels, GV, and TCM symptom scores (P < 0.05). Notably, the massage group showed significantly greater improvements in height, weight, BAI, IGF-1, and GV compared to the control group (P < 0.05). Furthermore, massage therapy was associated with a reduction in serum cortisol levels and an increase in LH and GH levels, indicating potential endocrine benefits.</p><p><strong>Conclusion: </strong>Pediatric massage combined with comprehensive management effectively promotes improvements in height, weight, and annual growth velocity (GV) among children with short stature.</p>","PeriodicalId":14131,"journal":{"name":"International Journal of General Medicine","volume":"18 ","pages":"4987-5000"},"PeriodicalIF":2.0,"publicationDate":"2025-08-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12410388/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145015146","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The Impact of School Education on High Myopia in Children and Adolescents.","authors":"Lurun Yu, Cancan Zhang, Yi Lu, Qi Gong, Ouwen Duan, Qing Yuan, Lianhong Zhou","doi":"10.2147/IJGM.S537179","DOIUrl":"10.2147/IJGM.S537179","url":null,"abstract":"<p><strong>Purpose: </strong>To investigate the effect of school education on the prevalence of high myopia.</p><p><strong>Patients and methods: </strong>This prospective cross-sectional study, conducted in schools across Hubei Province, included 1,017,622 students from 103 county-level administrative regions between October 2021 and November 2023. Refractive measurements and basic demographic data were collected for all participants. The prevalence of high myopia and the differences in prevalence across grade levels were calculated. Regression discontinuity was used to assess the effects of age and education on high myopia prevalence.</p><p><strong>Results: </strong>A total of 1,017,622 students participated, with 540,860 (53.15%) boys, and an average age of 11.93 ± 3.06 years. Over three years, the average prevalence of high myopia was 4.75%. Prevalence increased with grade level, with the largest difference observed between grades 9 and 10. Age showed no significant effect on high myopia prevalence. Each additional year of education led to an increase in prevalence by 1.26% (95% CI: -1.87, -0.65, P=0.000) in 2021, 1.20% (95% CI: -1.88, -0.52, P=0.001) in 2022, and 1.26% (95% CI: -2.49, -0.04, P=0.04) in 2023.</p><p><strong>Conclusion: </strong>Increased grade, rather than age, is the key factor driving the rise in high myopia prevalence. Interventions targeting myopia risk factors during schooling could potentially reduce the growing prevalence of high myopia.</p>","PeriodicalId":14131,"journal":{"name":"International Journal of General Medicine","volume":"18 ","pages":"4957-4964"},"PeriodicalIF":2.0,"publicationDate":"2025-08-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12410389/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145015189","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Changes in the Gut Microbiota of Neonates with Hyperbilirubinemia Reaching Phototherapy Thresholds.","authors":"Qiao Yu, Tiantian Lu, Jingjing Yan, Nan Shen, Rang Wu, Song Liu, Zhen Zhang, Suyue Zhu","doi":"10.2147/IJGM.S531481","DOIUrl":"10.2147/IJGM.S531481","url":null,"abstract":"<p><strong>Purpose: </strong>The gut microbiota plays a crucial role in bilirubin metabolism in neonates. The phototherapy threshold assesses the need for clinical intervention in neonatal hyperbilirubinemia (NH). This study aimed to investigate gut microbiota alterations in neonates with NH meeting the phototherapy threshold.</p><p><strong>Patients and methods: </strong>A total of 75 neonates with NH who met the phototherapy threshold (NH group), and 67 healthy neonates (control group) were included. Fecal samples were collected within one hour before initiating phototherapy for 16S rRNA gene sequencing and bioinformatic analysis.</p><p><strong>Results: </strong>In contrast to healthy controls, the NH group showed significantly higher Shannon (<i>p</i><0.001) and abundance-based coverage estimator (<i>p</i><0.05) indices, as well as significant differences in both unweighted and weighted UniFrac values (<i>p</i><0.001 for each). In addition, linear discriminant analysis effect size revealed significant taxonomic shifts in the gut microbiota of the NH group at multiple levels, including phylum, class, order, family, and genus. Among the key differential genera, the abundance of <i>Streptococcus</i> (<i>p</i><0.001) was significantly reduced, whereas <i>Escherichia</i> (<i>p</i><0.001) and <i>Klebsiella</i> (<i>p</i><0.001) were markedly enriched.</p><p><strong>Conclusion: </strong>Neonates meeting the phototherapy threshold exhibit altered gut microbiota composition, characterized by increased diversity, richness, and an elevated abundance of opportunistic pathogenic genera. These results offer valuable preliminary insights into the gut microbiome changes associated with NH requiring phototherapy.</p>","PeriodicalId":14131,"journal":{"name":"International Journal of General Medicine","volume":"18 ","pages":"5001-5011"},"PeriodicalIF":2.0,"publicationDate":"2025-08-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12413546/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145015132","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}