International journal of food microbiology最新文献

{"title":"Anti-listeria activity of newly isolated lactic acid bacteria from fermented foods and their application to smoked salmon(Coho)","authors":"Na-Yeon Son ,&nbsp;Gi-Seong Moon ,&nbsp;Mark S. Turner ,&nbsp;Hyun-Gyun Yuk","doi":"10.1016/j.ijfoodmicro.2025.111148","DOIUrl":"10.1016/j.ijfoodmicro.2025.111148","url":null,"abstract":"<div><div>The objective of this study was to evaluate the anti-listeria activities of lactic acid bacteria (LAB) isolated from Korean fermented foods and to assess the effect of fermentate (cells and cell-free supernatant [CFS]), CFS or cells in controlling <em>L. monocytogenes</em> on smoked salmon, packaged either in vacuum- and air-packaging. One <em>L. lactis</em> (CJNU3001) strain and two <em>Enterococcus faecium</em> (CJNU2524 and CJNU2008) strains appeared to have higher anti-listeria activity than the other 15 LAB strains. They also exhibited anti-listeria activity against 24 <em>L. monocytogenes</em> strains of various serotypes. CJNU3001 had greater anti-listeria activity in brain heat infusion broth than the two <em>E. faecium</em> strains. No anti-listeria activity was observed when the partially purified proteinaceous compounds of all three LAB strains were treated with proteinase K, revealing that their anti-listeria activities might be due to bacteriocins. Amplification and sequence analysis of the nisin gene confirmed that CJNU3001 was a nisin Z producer. The treatment of fermentate and CFS of CNJU3001 on smoked salmon inoculated with <em>L. monocytogenes</em> at 6.0 log CFU/g inhibited the growth of the pathogen during storage. On the other hand, about 2.0 log reduction was achieved immediately after treatment of the fermentate and CFS of CNJU3001 when <em>L. monocytogenes</em> was inoculated at 5.0 log CFU/g, regardless of storage temperature and packaging type. The treatment using only CJNU3001 cells was not effective in controlling <em>L. monocytogenes</em> on smoked salmon, regardless of inoculation level of the pathogen. The fermentate and CFS treatment of CJNU3001 did not cause any color change of vacuum-packed smoked salmon. Thus, this study suggests that CJNU3001 fermentate and CFS as biopreservatives could be used to control the growth of <em>L. monocytogenes</em> on smoked salmon without causing color change.</div></div>","PeriodicalId":14095,"journal":{"name":"International journal of food microbiology","volume":"434 ","pages":"Article 111148"},"PeriodicalIF":5.0,"publicationDate":"2025-03-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143580839","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Biological soil amendments can support survival of pathogenic and non-pathogenic Escherichia coli in soils and sporadic transfer to Romaine lettuce","authors":"Zirui Ray Xiong ,&nbsp;Ellen Gabriel ,&nbsp;Alan Gutierrez ,&nbsp;Cheryl East ,&nbsp;Kalmia E. Kniel ,&nbsp;Michelle D. Danyluk ,&nbsp;Michele Jay-Russell ,&nbsp;Manan Sharma","doi":"10.1016/j.ijfoodmicro.2025.111147","DOIUrl":"10.1016/j.ijfoodmicro.2025.111147","url":null,"abstract":"<div><div>Biological soil amendments (BSAs) are essential agricultural inputs that provide critical nutrients in organic leafy green production. Heat-treated poultry pellets (HTPP) and seabird guano (SBG), which have been treated to reduce microbial pathogen loads, are gaining popularity among growers. Using these BSAs in the process of side-dressing, providing nutrients to crops while growing, may provide opportunities for externally introduced bacterial pathogens to survive in soil. In this study, Romaine lettuce was grown in soils in a controlled environmental growth chamber. Soils were side-dressed twice with different combinations of treated BSAs: HTPP, SBG, and corn steep liquor (CSL). Soils were co-inoculated with non-pathogenic <em>E. coli</em> and two <em>E. coli</em> O157:H7 strains at the second of two side-dressing events. Survival of <em>E. coli</em> in soils over 28 days was evaluated. On day 28 post inoculation, two heads of Romaine lettuce from each planter were harvested, and the presence of <em>E. coli</em> on leaves was determined. Four nonlinear statistical models were fit to predict survival of <em>E. coli</em> in soils. In all soils regardless of BSA treatment, <em>E. coli</em> TVS 353 declined by 4.08–4.51 log CFU/g soil over 28 days, and <em>E. coli</em> O157:H7 declined by 2.77–4.3 log CFU/g soil over 28 days. <em>E. coli</em> TVS 353 and O157:H7 were recovered from 13.3 % (6/45) and 11.1 % (5/45) of plants, respectively. Transfer of <em>E. coli</em> from soils to Romaine lettuce was low, sporadic, and could not be measured quantitatively. Side-dressing with treated BSAs used in organic lettuce production supported but did not enhance survival of <em>E. coli</em> in side-dressed soils under controlled environmental conditions.</div></div>","PeriodicalId":14095,"journal":{"name":"International journal of food microbiology","volume":"434 ","pages":"Article 111147"},"PeriodicalIF":5.0,"publicationDate":"2025-03-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143563601","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Discovery of lactic acid bacteria with high nucleoside degradation and low purine production in tomato sour soup","authors":"Qingli Zhou ,&nbsp;Chaoya Zhao ,&nbsp;Xiefei Li ,&nbsp;Huiqun Wang ,&nbsp;Qun Huang ,&nbsp;Yong Sun ,&nbsp;Yan Zhou","doi":"10.1016/j.ijfoodmicro.2025.111139","DOIUrl":"10.1016/j.ijfoodmicro.2025.111139","url":null,"abstract":"<div><div>Tomato sour soup (TSS) is a traditional fermented food in the southwest of Guizhou province, China. In a previous study, we found that the purine nucleoside compound content in TSS was decreased after fermentation. In this study, we screened the nucleoside-degrading lactic acid bacteria in TSS and explored possible mechanisms for the degradation of purine nucleoside compound. <em>Lp. plantarum</em> ST-11 was chosen because of its strong guanosine and inosine degradation, low guanine and hypoxanthine production, safety, and probiotic characteristics. The whole genome sequence had 3,344,042 bp, and approximately 110 genes were related to nucleotide metabolism. Guanosine administration induced the downregulation of 26 metabolites and upregulation of 75 metabolites, which related to energy substances, purines, and pyrimidines et al. Thus, <em>Lp. plantarum</em> ST-11, with high nucleoside degradation and low purine production, was screened from TSS, as a potential probiotic to prevent hyperuricemia.</div></div>","PeriodicalId":14095,"journal":{"name":"International journal of food microbiology","volume":"434 ","pages":"Article 111139"},"PeriodicalIF":5.0,"publicationDate":"2025-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143552060","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Electroactivity of Shewanella putrefaciens induced by shrimp matrix: Catalyst for spoilage acceleration","authors":"Yilin Qian,&nbsp;Taige Liu,&nbsp;Liu Yang,&nbsp;Xianghong Meng,&nbsp;Fei Jia,&nbsp;Zunying Liu","doi":"10.1016/j.ijfoodmicro.2025.111119","DOIUrl":"10.1016/j.ijfoodmicro.2025.111119","url":null,"abstract":"<div><div>The bacterium <em>Shewanella</em> is commonly found in fishery products along the whole cold chain transportation system and poses a significant threat to public health and the global economy due to its propensity for contaminating food and causing spoilage. In this research, four specific spoilage organisms (SSO) (<em>Shewanella</em> spp.) isolated from various refrigerated aquatic products were found to exhibit electrochemical properties. When modifying the conventional microbial fuel cells with shrimp meat extract as the donor-acceptor, an interesting result was found in the current output of the “shrimp battery”, where it exhibits a significant activation effect and the accumulation of total volatile basic nitrogen, Trimethylamine N-oxide and bioamines. The transcriptomic analysis reveals that the extracellular electron transport pathway of <em>Shewanella putrefaciens</em>-329 in aquatic environments underwent a transfer from <em>Mtr</em> cluster to <em>cbb</em>_<em>3</em>-type, with its metabolic focus transitioning toward the accumulation of amines, sulfides, and biofilms. Our findings demonstrate that the electrochemical characteristics of <em>Shewanella</em> in aquatic environments play a crucial role in accelerating low-temperature spoilage of aquatic products, thereby offering a novel target for mitigating the detrimental loss of aquatic products caused by <em>Shewanella</em>.</div></div>","PeriodicalId":14095,"journal":{"name":"International journal of food microbiology","volume":"434 ","pages":"Article 111119"},"PeriodicalIF":5.0,"publicationDate":"2025-02-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143552059","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Characterization and release of casein‑sodium alginate embedding phage edible film and the application in controlling of Salmonella contamination in food","authors":"Peng Guo ,&nbsp;Lei Zhang ,&nbsp;Mengge Ning ,&nbsp;Ting Cai ,&nbsp;Fangyu Long ,&nbsp;Yahong Yuan ,&nbsp;Tianli Yue","doi":"10.1016/j.ijfoodmicro.2025.111137","DOIUrl":"10.1016/j.ijfoodmicro.2025.111137","url":null,"abstract":"<div><div><em>Salmonella</em> remains a major foodborne pathogen responsible for food poisoning and poses a serious threat to public health. This study has developed an edible antimicrobial film using bacteriophage (phage) SaTp-04, specifically designed to capture and eliminate <em>Salmonella</em> in food. Optimal film conditions were achieved using casein (CA) and sodium alginate (SA) as the matrix, glycerol as the plasticizer, and phage protector. The mechanical properties and phage encapsulation rate were used as response variables. The presence of phage SaTp-04 did not significantly alter the thickness, tensile strength, elongation at break, water vapor permeability, moisture content, or water solubility of the film. Furthermore, the Fourier Transform Infrared spectroscopy, X-ray diffraction, and microscopic morphology of the phage film were similar to those of the phage-free film. The phage titer in the film remained stable for up to five weeks at 4 °C. Phage release from the film in water followed a first-order kinetics model (y = 85.312 × (1-e^−0.106x), R² = 0.9687). The release amount and rate were lower at 4 °C on LB plate surfaces than at 25 °C. The phage film inhibited <em>Salmonella</em> growth on fresh chicken breast and carrots at 4 and 10 °C. The surface of chicken breast packaged with the phage film showed a reduction of approximately 6 log CFU/cm² in <em>Salmonella</em> compared to the control, while the carrot surface demonstrated a reduction in <em>Salmonella</em> to below the detection limit (10 CFU/cm²) at 37 °C. These results confirm the feasibility of using phage SaTp-04 CA–SA edible films for food safety, providing a viable strategy for developing targeted capture packaging for foodborne pathogenic bacteria.</div></div>","PeriodicalId":14095,"journal":{"name":"International journal of food microbiology","volume":"434 ","pages":"Article 111137"},"PeriodicalIF":5.0,"publicationDate":"2025-02-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143528690","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Mechanisms of action of bioprotective plant extracts against the ochratoxigenic Penicillium nordicum in dry-cured sausages","authors":"Elia Roncero ,&nbsp;Micaela Álvarez ,&nbsp;Josué Delgado ,&nbsp;Eva Cebrián ,&nbsp;María J. Andrade","doi":"10.1016/j.ijfoodmicro.2025.111133","DOIUrl":"10.1016/j.ijfoodmicro.2025.111133","url":null,"abstract":"<div><div>Plant extracts are promising strategies to minimise the hazard associated with ochratoxin A (OTA) in dry-cured sausages. Nonetheless, their mechanisms have not been elucidated yet. The mechanisms by which rosemary (REO), thyme (TEO) and oregano (OEO) essential oils and the extract of acorn shell (AE) impact on the OTA production by <em>Penicillium nordicum</em> were evaluated. Their effect on the mould's gene expression, ergosterol synthesis, reactive oxygen species (ROS) production and spore germination and their potential to the OTA detoxification were <em>in vitro</em> studied in three mould strains. Differences at sampling time, plant extract and mould strain levels were found for all mechanisms of the antifungal agents. The relative expression of <em>Hog1</em> and <em>Rho1</em> genes were less affected than that of the <em>otanps</em> and <em>otapks</em> genes in treated moulds compared to the non-treated control. All treatments were able to decrease ergosterol production against one strain. Furthermore, REO 500 μL/mL, AE and OEO 50 μL/mL produced the highest fluorescence associated with ROS for another strain at the end of the incubation. Moreover, a significant detoxification of the plant extracts was only found for OEO 50 μL/mL after 24 h of incubation. Besides, spore germination was totally inhibited by TEO and OEO, obtaining the opposite effect for AE. No clear relationship was found when those findings were correlated to the OTA accumulation. Consequently, the antiochratoxigenic effect of plant extracts seems to be due to the involvement of several mechanisms of action. Finally, the plant extracts did not inhibit the potentially protective cultures against ochratoxigenic moulds.</div></div>","PeriodicalId":14095,"journal":{"name":"International journal of food microbiology","volume":"434 ","pages":"Article 111133"},"PeriodicalIF":5.0,"publicationDate":"2025-02-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143563600","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Oil-in-water emulsion and acid concentrations impact Bacillus weihenstephanensis KBAB4 growth and culturability","authors":"Agathe Dutoit,&nbsp;Clément Trunet,&nbsp;Nicolas Decourcelle,&nbsp;Anne-Gabrielle Mathot,&nbsp;Louis Coroller","doi":"10.1016/j.ijfoodmicro.2025.111132","DOIUrl":"10.1016/j.ijfoodmicro.2025.111132","url":null,"abstract":"<div><div>Organic acids have long been used to limit microbial growth and preserve food products from spoilage and pathogens. However, the food complexity can affect the efficiency of preservatives in inhibiting bacterial growth. The inhibitory activity of acetic, lactic, caprylic and lauric acid in nutrient broth and in a model food system, i.e. an oil-in-water (O/W) emulsion at two pH levels, were compared using the plate count method.</div><div>The fate of <em>B. weihenstephanensis</em> KBAB4 cells incubated in O/W emulsions revealed a loss of culturability on Brain Heart Infusion agar (BHI agar) medium, occurring at different times according to the tested conditions. Cell viability in O/W emulsion was assessed through flow cytometry measurements which confirmed that a large majority of cells possessed an intact membrane, despite only 3 % being able to recover on BHI agar. Decreasing the pH of the emulsions delayed the time at which cells lost their culturability as well as the time at which it was restored. Using acetic, caprylic and lauric acid in the emulsion impacted strain recovery on BHI agar whereas using lactic acid enhanced the cell recovery from the emulsion exposure, on BHI agar surface.</div><div>The culturability of <em>B. weihenstephanensis</em> KBAB4 cells incubated in an O/W emulsion without acid has been restored using other recovery media, highlighting ROS involvement and limited strain catalase activity in the recovery ability. This study put in evidence the susceptibility of this strain to oxidative stress and challenges the use of given media for enumerating spore-forming bacteria in food and other complex matrices.</div></div>","PeriodicalId":14095,"journal":{"name":"International journal of food microbiology","volume":"434 ","pages":"Article 111132"},"PeriodicalIF":5.0,"publicationDate":"2025-02-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143580837","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Genomic features and pathogenicity of atypical diarrheagenic Escherichia coli from a large foodborne outbreak","authors":"Kenji Ohya ,&nbsp;Shouhei Hirose ,&nbsp;Kohei Nishikaku ,&nbsp;Takahiro Ohnishi ,&nbsp;Kenichi Lee ,&nbsp;Sunao Iyoda ,&nbsp;Akiko Kubomura ,&nbsp;Yukihiro Akeda ,&nbsp;Katsumi Mizukami ,&nbsp;Tomikatsu Suzuki ,&nbsp;Kenji Takinami ,&nbsp;Yuhji Taquahashi ,&nbsp;Makiko Kuwagata ,&nbsp;Satoshi Kitajima ,&nbsp;Takashi Inoue ,&nbsp;Yukiko Hara-Kudo","doi":"10.1016/j.ijfoodmicro.2025.111134","DOIUrl":"10.1016/j.ijfoodmicro.2025.111134","url":null,"abstract":"<div><div>An outbreak of diarrheal illness related to milk cartons served in school lunches, occurred in June 2021, involving more than 1800 cases from 25 schools. A strain of <em>Escherichia coli</em> OUT (OgGp9):H18 was implicated in the outbreak. This strain does not possess virulence factors typical of other <em>E. coli</em> pathotypes. In this study, we examined the pathogenicity of the <em>E. coli</em> OUT (OgGp9):H18 strain using genomic analysis and animal models. A core genome-based phylogenetic analysis revealed that this strain belongs to a clade comprising ST1380 strains and is distinct from enteroaggregative <em>E. coli</em> 042 and uropathogenic <em>E. coli</em> UMN026, which were previously considered to be phylogenetically related to this strain. In addition, the strain harbors a plasmid similar to that of atypical enterotoxigenic <em>E. coli</em>, encoding Coli Surface antigen CS8 and a type VI secretion system (T6SS). The strain caused mortality in mice following intraperitoneal inoculation. Marmosets inoculated orally, experienced diarrhea and long-term shedding. Curing the strain of the 103 Kbp plasmid it carries reduced mortality rates and colonization in the experimental animals, indicating that the plasmid encodes virulence factors. However, the mortality of mice treated with the plasmid-cured strain was higher than that of those treated with nonvirulent <em>E. coli</em> K-12, indicating that the chromosome also encodes virulence factors. Identified chromosomal virulence factors include a T6SS, the second type III secretion system in <em>E. coli</em>, ETT2, and the capsule gene cluster <em>kps</em>. These findings suggest that atypical diarrheagenic <em>E. coli</em>, such as the strain investigated in this study, may be the cause of foodborne illness in patients with diarrhea with an unknown cause.</div></div>","PeriodicalId":14095,"journal":{"name":"International journal of food microbiology","volume":"434 ","pages":"Article 111134"},"PeriodicalIF":5.0,"publicationDate":"2025-02-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143552058","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Mycotoxigenic fungi, OTA and fumonisin B2 production by Aspergillus section Nigri isolated from wine grapes and natural occurrence of OTA in wines of Northern Argentinean Patagonia","authors":"Carolina Temperini ,&nbsp;Minna Kemppainen ,&nbsp;Mónica Moya ,&nbsp;Mariana Greco ,&nbsp;Alejandro Pardo ,&nbsp;Graciela Pose","doi":"10.1016/j.ijfoodmicro.2025.111135","DOIUrl":"10.1016/j.ijfoodmicro.2025.111135","url":null,"abstract":"<div><div>The Northern Argentinean Patagonia is the southernmost producing region of wine grapes and wine in Argentina. Their wines possess distinctive features due to the wide variety of red and white grapes and weather conditions, achieving great acceptance in local and international markets. The aims of the present study were to determine and characterize the mycobiota present in wine grapes from Northern Patagonia, to analyse the ability of the isolates to produce mycotoxins, and to determine the natural occurrence of OTA and monitoring its presence in wine over time. A wide diversity of fungal contamination was found on grape berries surface. The most abundant genera isolated were: <em>Alternaria</em> (55.8 %), <em>Aspergillus</em> (12.7 %), <em>Trichoderma</em> (6.5 %), <em>Penicillium</em> (5.3 %), <em>Fusarium</em> (4.4 %), <em>Epiccocum</em> (4.2 %), <em>Acremonium</em> (2.6 %) and <em>Botrytis</em> (2.5 %). <em>Alternaria</em> was the predominant genus with the highest proportion during the three years of study and <em>A. tenuissima</em> was the most frequent species isolated. In contrast, a low incidence of <em>Aspergillus</em> section <em>Nigri</em> was found. Isolates identified belonged to <em>Aspergillus niger</em> aggregate and, more specifically, to <em>A. tubingensis</em> species<em>.</em> No <em>Aspergillus carbonarius</em> isolates were found. A low percentage of <em>A. tubingensis</em> isolates were ochratoxin A producers (4.7 %) in the range of 14.6–233.3 μg/Kg and FB2 producers (3.7 %) in the range of 79.7–277.8 μg/Kg. None of the OTA producer isolates were able to produce FB2 and vice versa. Analysis of OTA content in wine samples from Northern Patagonia showed negative results: OTA was not detected in any of the samples analysed. Our findings suggest a low risk of wine contamination with OTA.</div></div>","PeriodicalId":14095,"journal":{"name":"International journal of food microbiology","volume":"433 ","pages":"Article 111135"},"PeriodicalIF":5.0,"publicationDate":"2025-02-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143510107","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Development of predictive models of biofilm formation by C. sakazakii, E. cloacae on surfaces used in the food industry and medicine","authors":"Mateusz Gemba,&nbsp;Elżbieta Rosiak,&nbsp;Danuta Kołożyn-Krajewska","doi":"10.1016/j.ijfoodmicro.2025.111131","DOIUrl":"10.1016/j.ijfoodmicro.2025.111131","url":null,"abstract":"<div><div><em>Cronobacter sakazakii</em> and <em>Enterobacter cloacae</em> exhibit the ability to form biofilms, making them resistant to drying, antibiotics, and changes in pH. These biofilms can adhere to different surfaces, including tissues, catheters, enteral feeding tubes, and work surfaces, potentially leading to cross-infection risks in both the food and medical sectors. The objective of this study was to develop a predictive model of biofilm formation over time by <em>C. sakazakii</em> and <em>E. cloacae</em> on medical polyvinyl chloride (PVC) at 37 °C and stainless steel (SS), polypropylene (PP) surfaces at 4 °C and different microbial inoculum concentration. A staining method and spectrophotometric measurement were used to assess biofilm formation. SyStat Software Inc. for Windows Table curve 3D v.4.0.05 and non-linear functions were used to develop predictive models. Analysis of biofilm formation on SS and PP surfaces at 4 °C by all analyzed bacteria suggests that hygiene procedures in refrigeration equipment should be performed daily, the maximum safe storage time for bottled milk is 24 h. At 37 °C <em>E. cloacae</em> posed the highest risk of biofilm formation on the surface of PVC tubing at 6–36 h. The six best response surface models of biofilm formation were selected for presentation. The majority of these models demonstrated good accuracy, as evidenced low mean standard errors (MSE), high coefficient R² and Adjusted R(<span><span>Aung and Chang, 2014</span></span>²⁾. These models can be utilized to evaluate the microbiological risks in settings such as human milk banks, neonatal intensive care units and food industry plants.</div></div>","PeriodicalId":14095,"journal":{"name":"International journal of food microbiology","volume":"434 ","pages":"Article 111131"},"PeriodicalIF":5.0,"publicationDate":"2025-02-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143528691","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}