International journal of food microbiology最新文献

{"title":"Ultrasonic-assisted preparation of rose oil nanoemulsion: Characterization, anti-Escherichia coli activity, and its application in cucumber juice","authors":"Yue Zhang ,&nbsp;Yulin Liu ,&nbsp;Ruiping Wang ,&nbsp;Qi He ,&nbsp;Xiaoyi Ding ,&nbsp;Fangkui Wang ,&nbsp;Yin Zhou","doi":"10.1016/j.ijfoodmicro.2025.111191","DOIUrl":"10.1016/j.ijfoodmicro.2025.111191","url":null,"abstract":"<div><div>In this study, six rose oil nanoemulsions (RONs) were prepared under different ultrasonic power preparation conditions (65w, 91w, 97.5w, 130w, 162.5w, and 195w), naming RON-65w, RON-91w, RON-97.5w, RON-130w, RON-162.5w, RON-195w, respectively. The particle size, and zeta potential revealed that the mean particle size of RONs decreased with the rising of ultrasonic power when RONs were freshly prepared. RONs had storage stability for at least 30 days, and the particle size variations of RON-65w and RON-97.5w were smaller than other RONs. Time-killing assay, minimum inhibitory concentration (MIC), and growth curve analysis revealed that RONs exhibited higher antibacterial activities against <em>Escherichia coli</em> than rose oil (RO). Scanning electron microscopy and bacterial membrane integrity analysis revealed that RO and RONs exerted their antibacterial effect by increasing the permeability of bacterial cells, and the leakage of nucleic acids and protein caused by RONs were higher than RO (<em>p</em> &lt; 0.05). Compared with MIC assay, RON-65w and RON-97.5w exhibited significantly higher antibacterial activities than other RONs in growth curve analysis, and RON-65w led to highest leakage of nucleic acids and protein among all RONs in bacterial membrane integrity analysis (<em>p</em> &lt; 0.05). Thus, the effect of ultrasonic power on antibacterial activity of RONs was more obvious under agitated conditions than static experimental conditions. The results of application experiment indicated that RON-97.5w displayed greater inhibition ability against <em>E.coli</em> than RO in cucumber juice. Based on the results of this study, rose oil nanoemulsions can be applied as natural preservatives in liquid foods to prevent bacteria contamination.</div></div>","PeriodicalId":14095,"journal":{"name":"International journal of food microbiology","volume":"436 ","pages":"Article 111191"},"PeriodicalIF":5.0,"publicationDate":"2025-04-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143816934","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Sublethal sanitizers exposure differentially affects biofilm formation in three adapted Salmonella strains: A phenotypic-transcriptomic analysis of increased biofilm formed by ATCC 14028","authors":"Xiaoxue Yan ,&nbsp;Rongfeng Tao ,&nbsp;Hongyuan Zhou ,&nbsp;Yuhao Zhang ,&nbsp;Dong Chen ,&nbsp;Liang Ma ,&nbsp;Yamin Bai","doi":"10.1016/j.ijfoodmicro.2025.111189","DOIUrl":"10.1016/j.ijfoodmicro.2025.111189","url":null,"abstract":"<div><h3>Purpose</h3><div>Using sanitizer in food industry is an important mean of sterilization and biofilm eradication, but inappropriate operation may lead to resistance, posing a concealed risk to food safety. The purpose of this study was to assess the impact of sub-lethal sanitizers on the biofilm formed by adaptive <em>Salmonella</em> and to explore the variations in transcription within adaptive <em>Salmonella</em> biofilms when co-incubated with sublethal concentrations of sanitizers.</div></div><div><h3>Methods</h3><div>The microbroth dilution method was determined to measure the MIC of three sanitizers on <em>Salmonella</em>, and adaptation induction was conducted with steadily increasing sanitizer concentrations. The effect of sub-MIC sanitizers on the biofilm of <em>Salmonella</em> was investigated by crystal violet method, confocal laser scanning microscopy and transcriptomics.</div></div><div><h3>Results</h3><div>The results indicated that the maximum growth concentration of the adapted strains was 1.69–43.25 times that of the original MIC, and the number of bacteria and matrix content were increased when re-exposed to sub-MIC benzalkonium chloride (BZK), and the expression of regulatory factors and various amino acid biosynthesis and metabolism-related genes showed an up-regulation trend.</div></div><div><h3>Significance</h3><div>This will be beneficial to clarify the correlation and mechanism between the sanitizer adaptation of salmonellae caused by improper sanitization and increased biofilm formation resulting from this adaptation. And it helps to adjust the appropriate dosage of sanitizer and optimize sanitation standard operating procedures (SSOP) in the foodstuff industry, thereby effectively promoting the bactericidal effect and eliminating foodborne pathogens' biofilm.</div></div>","PeriodicalId":14095,"journal":{"name":"International journal of food microbiology","volume":"436 ","pages":"Article 111189"},"PeriodicalIF":5.0,"publicationDate":"2025-04-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143824437","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Dictyophora indusiata polysaccharide enhances flavor and viability of probiotic Bifidobacterium longum F2 in milk beverage","authors":"Zhikun Zeng,&nbsp;Shuangan Chen,&nbsp;Lin Wang,&nbsp;Yaqing Lin,&nbsp;Baodong Zheng,&nbsp;Yi Zhang,&nbsp;Lei Pan","doi":"10.1016/j.ijfoodmicro.2025.111190","DOIUrl":"10.1016/j.ijfoodmicro.2025.111190","url":null,"abstract":"<div><div><em>Dictyophora indusiata</em> polysaccharide (DIP) is a bioactive substance with excellent prebiotic activity. In this study, we investigated the effects of 0.1 % (<em>w</em>/<em>v</em>) DIP on the physical properties, flavor characteristics and <em>Bifidobacterium longum</em> F2 proliferation of milk beverages. The results showed that DIP maintained the stability of milk beverages, and improved the flavor of milk beverages, including the sweetness, sourness and umami. Notably, DIP significantly increased the content of acetoin, 2-nonanone and 1-nonanol on the 1st and 7th days of storage in milk beverages. In particular, DIP promoted the proliferation of <em>Streptococcus thermophilus</em>, <em>Lactobacillus bulgaricus</em>, <em>Bifidobacterium longum</em> F2 and produced acetic acid, which contributed to the improvement of the nutritional properties of the milk beverages. Taken together, the impact of DIP on physical properties, flavor and acetic acid production of milk beverages are consistent with the sensory results. This study demonstrates that DIP is a promising milk beverage additive and provides theoretical guidance for the commercialization of DIP for high value utilization.</div></div>","PeriodicalId":14095,"journal":{"name":"International journal of food microbiology","volume":"436 ","pages":"Article 111190"},"PeriodicalIF":5.0,"publicationDate":"2025-04-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143816935","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Multidrug-resistant, hyperaerotolerant, and aerobic growth of Campylobacter jejuni and C. coli in retail broiler chicken meat in Selangor, Malaysia","authors":"Kah Hui Chong ,&nbsp;Nor Ainy Mahyudin ,&nbsp;Hanan Hasan ,&nbsp;Chia Yeung New ,&nbsp;Nur-Shahera Mohammad Sabri ,&nbsp;Jiang Shan ,&nbsp;Kousalya Padmanabhan ,&nbsp;Nor Azmiraah Abd Jabar ,&nbsp;Wanyu Pan ,&nbsp;Epeng Lee ,&nbsp;Yuli Haryani ,&nbsp;Son Radu ,&nbsp;Mahmud Ab Rashid Nor-Khaizura","doi":"10.1016/j.ijfoodmicro.2025.111187","DOIUrl":"10.1016/j.ijfoodmicro.2025.111187","url":null,"abstract":"<div><div><em>Campylobacter</em> spp. are one of the major zoonotic pathogens from chicken meat, causing world‑leading foodborne diseases. Thus, the aims of this study include monitoring the aerobic plate count (APC), the prevalence, and the characterisations (antimicrobial resistance, aerotolerance, aerobic growth, and plasmid profiling) of <em>Campylobacter</em> spp.. This study sampled 260 chicken thighs and drumsticks from retail supermarkets and wet markets in Selangor, Malaysia. Prevalence and microbial loads of <em>Campylobacter</em> spp. were assessed with the most probable number-polymerase chain reaction (MPN-PCR). Antibiotic resistance assay was conducted with disk diffusion assay with 12 types of antibiotics. Prevalence surveillance indicated that 68.9 % of <em>Campylobacter</em> spp., 47.7 % of <em>C. jejuni</em>, and 40.0 % of <em>C. coli</em> were detected. Co-contamination prevalence of <em>C. jejuni</em> and <em>C. coli</em> (28.46 %) occurred significantly higher than mono-contaminations, 19.62 % in <em>C. jejuni</em> and 11.54 % in <em>C. coli</em>, respectively. The microbial loads of <em>Campylobacter</em> spp., <em>C. jejuni</em>, and <em>C. coli</em> were 1.02 ± 0.83 log MPN/g, 0.60 ± 0.57 log MPN/g, and 0.53 ± 0.58 log MPN/g, respectively. <em>C. jejuni</em> and <em>C. coli</em> showed extremely high resistance against nalidixic acid, ciprofloxacin, cephalothin, and ampicillin. <em>Campylobacter</em> spp. isolated exhibited aberrance from their microaerophilic nature, with 87.5 % of <em>C. jejuni</em> being HAT (hyperaerotolerant) and 12.5 % of <em>C. jejuni</em> were AT (aerotolerant), while 100 % of <em>C. coli</em> were HAT. Alarmingly, the aerobic condition did not confine the growth, as 18.75 % of <em>C. jejuni</em> demonstrated aerobic growth. Hence, our study accentuates the current risk profile of <em>C. jejuni</em> and <em>C. coli</em> from retail broiler chicken meat.</div></div>","PeriodicalId":14095,"journal":{"name":"International journal of food microbiology","volume":"436 ","pages":"Article 111187"},"PeriodicalIF":5.0,"publicationDate":"2025-04-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143816936","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Impact of solid-state fermentation inoculated with Eurotium cristatum on the main composition and flavor of three kinds of Pu-erh tea","authors":"Mengqin Wang ,&nbsp;Da Xu ,&nbsp;Yuan Xu ,&nbsp;Wenwei Zheng ,&nbsp;Shuyin Zhang ,&nbsp;Liyuan Luo ,&nbsp;Jiangong Wen ,&nbsp;Yunfeng Zhang","doi":"10.1016/j.ijfoodmicro.2025.111186","DOIUrl":"10.1016/j.ijfoodmicro.2025.111186","url":null,"abstract":"<div><div>This study aims to address the problems of strong bitterness, astringency, and inferior taste in Pu'er tea made from young tea trees. First, four strains of <em>Eurotium cristatum</em> were isolated and screened by the static suspension isolation method using tea infusion as the culture medium and identified through morphological and molecular analysis. One of the strains was selected for the solid - state fermentation of raw Pu'er tea, Pu'er black tea, and Pu'er green tea. The fermentation conditions were optimized via response - surface experimental design. The results showed that the optimized fermentation time, temperature, and relative humidity for the three types of tea were 10 days, 30 °C, 45 %; 5 days, 30 °C, 40 %; and 10 days, 35 °C, 40 % respectively. Sensory evaluation indicated that after fermentation, the color of the tea leaves and tea soup deepened. And the freshness of raw Pu'er tea and green Pu'er tea decreased, while a subtle moldy aroma aroma was introduced after fermentation. The astringency and bitterness were effectively reduced. Chemical analysis revealed that, except for the stable caffeine level, the levels of components such as tea polyphenols and catechins changed significantly. Correlation analysis demonstrated a strong correlation between the color, aroma, and taste of the tea and the contents of various chemical components. GC - MS analysis identified 370 volatile compounds, 262 of which showed differences before and after fermentation. In conclusion, solid - state fermentation with <em>E.cristatum</em> can affect the chemical composition of the three types of tea, improve their taste and flavor, and offer a new approach for enhancing the quality of tea from young tea trees.</div></div>","PeriodicalId":14095,"journal":{"name":"International journal of food microbiology","volume":"436 ","pages":"Article 111186"},"PeriodicalIF":5.0,"publicationDate":"2025-04-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143839443","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Comparative analysis of virulence in Listeria monocytogenes: Insights from genomic variations and in vitro cell-based studies","authors":"Yiyang Song ,&nbsp;Binru Gao ,&nbsp;Hua Cai ,&nbsp;XiaoJie Qin ,&nbsp;XueJuan Xia ,&nbsp;Qingli Dong ,&nbsp;Takashi Hirata ,&nbsp;Zhuosi Li","doi":"10.1016/j.ijfoodmicro.2025.111188","DOIUrl":"10.1016/j.ijfoodmicro.2025.111188","url":null,"abstract":"<div><div><em>Listeria monocytogenes</em> is a significant foodborne pathogen capable of crossing the intestinal barrier and causing invasive listeriosis. This study focused on 37 <em>L. monocytogenes</em> strains isolated from food and clinical samples in the Shanghai region between 2020 and 2023. The <em>in vitro</em> cell models were used to assess the bacteria's ability to cross the intestinal barrier, as well as their adhesion and invasion capabilities in placental cells. Whole-genome analysis of the bacterial strains was also performed. The results showed that strains from lineage I exhibited significantly higher translocation ability across the Caco-2 barrier, as well as higher adhesion and invasion rates in JEG-3 cells, compared to strains from lineage II. Strains from serogroup IIb demonstrated significantly higher adhesion and invasion rates in JEG-3 cells than those from serogroups IIa and IIc. Clinical isolates had significantly higher translocation ability across the Caco-2 barrier, and higher adhesion and invasion rates in JEG-3 cells, compared to food-derived strains. Mutations in the <em>inlA</em> gene were detected in ST9 and ST121 strains, which may be a potential cause of the reduced virulence observed in these ST types. The presence of LIPI-4 in ST87 strains and LIPI-3 in ST3 strains may also explain the high virulence of these ST types strains. The results of this study, by integrating <em>in vitro</em> virulence phenotypes with genomic data, further enhance the understanding of virulence differences among different bacterial strains.</div></div>","PeriodicalId":14095,"journal":{"name":"International journal of food microbiology","volume":"435 ","pages":"Article 111188"},"PeriodicalIF":5.0,"publicationDate":"2025-04-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143783070","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Initial acidity regulates microbial sulfur metabolism in the spontaneous fermentation of sesame flavor-type baijiu","authors":"Xueao Ji ,&nbsp;Xiaowei Yu ,&nbsp;Yan Xu ,&nbsp;Qun Wu ,&nbsp;Meysam Madadi ,&nbsp;Amin Mousavi Khaneghah","doi":"10.1016/j.ijfoodmicro.2025.111182","DOIUrl":"10.1016/j.ijfoodmicro.2025.111182","url":null,"abstract":"<div><div>Volatile sulfur compounds play a crucial role in fermented foods, however, their metabolism during spontaneous food fermentation remains underexplored. With 3-(methylthio)-1-propanal as a case, we revealed the effect of initial acidity on the sulfur metabolism during sesame flavor-type <em>baijiu</em> fermentation. Results showed that the content of 3-(methylthio)-1-propanal reached 383.29 μg/kg with 1.8 mmol/10 g of the initial acidity, and it decreased to 320.54 μg/kg when the initial acidity increased to 2.4 mmol/10 g. Metagenomic analysis identified 11 core microbes associated with sulfur metabolism, characterized by gene abundance (&gt; 0.5 %) and catalytic enzyme distribution frequency (&gt; 10 %). <em>Saccharomyces</em> was the main producer of sulfur compounds. <em>Lactobacillus</em> was an important player in the methyl cycle pathway of sulfur metabolism. Low initial acidity increased the abundance of <em>Lactobacillus</em> and the content of genes associated with the methyl cycle pathway (<em>P</em> &lt; 0.05). This resulted in a significant increase of methionine (253.26 mg/kg) (<em>P</em> &lt; 0.05) and 3-(methylthio)-1-propanal contents (383.29 μg/kg) (<em>P</em> &lt; 0.05). In simulated fermentation, the content of 3-(methylthio)-1-propanal significantly increased by 2.91 folds when <em>Saccharomyces cerevisiae</em> was cocultured with <em>Lactobacillus acetotolerans</em> (<em>P</em> &lt; 0.05), and the transcription of genes related to sulfur metabolism significantly increased by 33.71 folds (<em>P</em> &lt; 0.05). Results indicated that low initial acidity increased the abundance of <em>Lactobacillus</em>, which mediates the methyl cycle pathway in the sulfur metabolism, thereby increasing the contents of methionine and volatile sulfur compounds. This work provided insight into the regulation of metabolic mechanisms of volatile sulfur compounds in <em>baijiu</em> fermentation.</div></div>","PeriodicalId":14095,"journal":{"name":"International journal of food microbiology","volume":"435 ","pages":"Article 111182"},"PeriodicalIF":5.0,"publicationDate":"2025-03-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143738635","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effect of CFEM proteins on pathogenicity, patulin accumulation and host immunity of postharvest apple pathogens Penicillium expansum","authors":"Yanling Wang,&nbsp;Jie Gao,&nbsp;Xin Chen,&nbsp;Yanqi Huang,&nbsp;Yurui Wu,&nbsp;Jinfen Zhu,&nbsp;Wei Li","doi":"10.1016/j.ijfoodmicro.2025.111180","DOIUrl":"10.1016/j.ijfoodmicro.2025.111180","url":null,"abstract":"<div><div><em>Penicillium expansum</em> is a significant post-harvest pathogenic fungi on most pome fruits. Common fungal extracellular membrane (CFEM) proteins, as effectors, contribute to virulence and manipulate host immunity. However, the CFEM proteins in <em>P. expansum</em> have not been identified and functionally studied. In this study, we screened two <em>P. expansum</em> CFEM proteins, PeCFEM5 and PeCFEM8, whose expression was highly up-regulated during postharvest apple infection. Growth and pathogenicity of <em>P. expansum</em> were characterized by knockout and complementary of <em>PeCFEM5</em> and <em>PeCFEM8</em>. Deletion of <em>PeCFEM5</em> and <em>PeCFEM8</em> resulted in changes in spore development and increased resistance to cell wall integrity stress. The lesion spots on apple and pear fruit inoculated with <em>P. expansum</em> gradually expanded and deepened in color. The Δ<em>PeCFEM5</em> and Δ<em>PeCFEM8</em> strains reduced lesion diameter on apple fruit by 47 % and 29 %, respectively, compared with the WT strains. Detection of patulin accumulation by high-performance liquid chromatography (HPLC) revealed that deletion of <em>PeCFEM5</em> or <em>PeCFEM8</em> suppressed patulin content in medium and apples, and patulin biosynthesis-related genes were down-regulated. The PeCFEM5 and PeCFEM8 were also confirmed as effector proteins capable of suppressing the cell death triggered by BAX and the expression of plant defense genes in <em>Nicotiana benthamiana</em>. Phytohormone ELISA assays showed that jasmonic acid levels were reduced, but salicylic acid levels were increased by transient expression of PeCFEM5 or PeCFEM8 in the host plant. These results indicate that PeCFEM5 and PeCFEM8 effectors are crucial for pathogenicity, patulin biogenesis, and modulating host plant immunity.</div></div>","PeriodicalId":14095,"journal":{"name":"International journal of food microbiology","volume":"435 ","pages":"Article 111180"},"PeriodicalIF":5.0,"publicationDate":"2025-03-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143748307","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Emergence of multidrug resistant Escherichia coli coharboring fosA3 and ESBL genes from retail ducks along slaughter line","authors":"Jialan Chen ,&nbsp;Ruyi Kuang ,&nbsp;Haotian Liu ,&nbsp;Lei Yang ,&nbsp;Jiarui Li ,&nbsp;Junlin Li ,&nbsp;Jing Xia ,&nbsp;Yong Huang ,&nbsp;Min Cui ,&nbsp;Likou Zou ,&nbsp;Xinfeng Han","doi":"10.1016/j.ijfoodmicro.2025.111185","DOIUrl":"10.1016/j.ijfoodmicro.2025.111185","url":null,"abstract":"<div><div>The excessive and indiscriminate use of antimicrobial agents in poultry production has increased antimicrobial resistance in <em>E. coli</em>, posing a significant threat to public health. This study investigated the prevalence of multidrug-resistant (MDR) <em>E. coli</em> co-harboring <em>fosA3</em> and extended-spectrum β-lactamases (ESBLs) genes in large-scale retail duck slaughterhouses in Sichuan Province, China. The antimicrobial susceptibility to 9 categories and 16 types of antimicrobial agents was assessed by using the broth microdilution method. Phylogenetic grouping and pulsed-field gel electrophoresis (PFGE) were used to investigate the phylogenetic groups and genetic characteristics of the isolates. Moreover, whole-genome sequencing (WGS) was performed on 35 representative <em>E. coli</em> isolates. Among 1, 059 samples collected, 895 <em>E. coli</em> were isolated by MacConkey (MAC) agar and eosin methylene blue (EMB) agar and PCR identification, and 150 strains of MDR <em>E. coli</em> co-carrying <em>fosA3</em> and ESBL genes were finally screened for further analysis. The results indicated that 141 isolates were resistant to fosfomycin and exhibited high resistance to β-lactam antibiotics. All isolates demonstrated MDR patterns, with 148 isolates resistant to six or more classes of antimicrobial agents. The majority (70/150, 46.67 %) belonged to phylogenetic group B1. Based on an 85 % similarity threshold, all isolates were categorized into 40 distinct PFGE types. Genomic analysis revealed 27 different serotypes and 19 sequence types (STs), with O8 (14.29 %, 5/35) and ST155 (22.86 %, 8/35) being the most common. The IncFIB (AP001918) was the most prevalent plasmid replicon type, identified in 74.29 % (26/35) of the isolates. In addition, 49 acquired antimicrobial resistance genes (ARGs) associated with resistance to 11 types of antimicrobial agents were identified and chromosomal mutation of p.S83L in <em>gyrA</em> gene (88.57 %, 31/35) was the most common. Furthermore, 110 virulence factors (VFs) were identified, with those related to iron uptake and storage, adhesion, secretion and endotoxin production being the most prevalent. This study underscored the importance of rational use of antimicrobial agents in poultry farming and provided critical insights into the distribution of ARGs and VFs among retail duck-derived MDR <em>E. coli</em>.</div></div>","PeriodicalId":14095,"journal":{"name":"International journal of food microbiology","volume":"435 ","pages":"Article 111185"},"PeriodicalIF":5.0,"publicationDate":"2025-03-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143748309","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The deletion of the uvrY in Aeromonas veronii disrupted the BarA/UvrY two-component system, decreasing persister formation and bacterial resistance to multiple antibiotics","authors":"Jingfei Long ,&nbsp;Hongzhou Xu ,&nbsp;Xiaoyu Qi ,&nbsp;Chenyang Yan ,&nbsp;Xiaonan Sun ,&nbsp;Yuanjiang Jin ,&nbsp;Xiaoqiang Liu ,&nbsp;Haixia Liu","doi":"10.1016/j.ijfoodmicro.2025.111183","DOIUrl":"10.1016/j.ijfoodmicro.2025.111183","url":null,"abstract":"<div><div>Antibiotic resistance (AR) is increasingly recognized as a critical global public health threat. <em>Aeromonas</em> species, widely distributed in aquatic environments, have emerged as potential foodborne pathogens. These bacteria are frequently detected in water sources and various ready-to-eat foods, posing a significant risk to food safety and human health. Two-component systems (TCSs) are key regulators of stress tolerance and adaptive behaviors, but the role of the BarA-UvrY TCS in AR is unclear. In our study, multidrug-resistant <em>Aeromonas veronii</em> (<em>A. veronii</em>) strains isolated from the grass carp intestinal contents were used to investigate the role of <em>uvr</em>Y in AR, and mutant strain (Δ <em>uvr</em>Y) was constructed using homologous recombination. The growth characteristics of wild-type (WT), Δ <em>uvr</em>Y, and complemented strains (C-Δ <em>uvr</em>Y) were evaluated under various stress conditions. Additionally, prokaryotic transcriptome analysis was performed to identify the downstream stress-factors in WT and Δ <em>uvr</em>Y. The results indicated that the Δ <em>uvr</em>Y strain exhibited reduced tolerance to osmotic and acid - base stress compared with the WT and C-Δ <em>uvr</em>Y. Furthermore, the deletion of <em>uvr</em>Y in <em>A. veronii</em> significantly impaired persister formation and decreased resistance to multiple antibiotics, particularly tetracyclines and chloramphenicol. The transcriptome analysis revealed that the increased susceptibility of Δ <em>uvr</em>Y to tetracyclines was accompanied by a significant down-regulation of efflux pump genes and NADH dehydrogenase I. STRING network analysis further demonstrated that the BarA-UvrY TCS is associated with genes encoding NADH dehydrogenase I and efflux pump. Additionally, efflux experiments and respiratory rate assays confirmed that the Δ <em>uvr</em>Y strain exhibited reduced efflux pump activity and a low respiratory rate, establishing a clear correlation between these two processes. Collectively, BarA-UvrY TCS play a crucial role in AR and persister formation by mediating energy-dependent efflux mechanisms. This study provides mechanistic insights into the regulatory functions of UvrY and offers a theoretical foundation for developing novel strategies to control <em>A. veronii</em> infections and enhance antimicrobial interventions.</div></div>","PeriodicalId":14095,"journal":{"name":"International journal of food microbiology","volume":"435 ","pages":"Article 111183"},"PeriodicalIF":5.0,"publicationDate":"2025-03-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143738637","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}