International Journal of Artificial Organs最新文献

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Effect of surface coatings on endothelialization and biofilm in PTFE vascular grafts.
IF 1.4 4区 医学
International Journal of Artificial Organs Pub Date : 2025-02-04 DOI: 10.1177/03913988251316438
Muhammet Hüseyin Erkan, Mehmet Boğa, Hanife Salih, Emin Barbarus, Ömer Faruk Rahman, Sarhan Sakarya
{"title":"Effect of surface coatings on endothelialization and biofilm in PTFE vascular grafts.","authors":"Muhammet Hüseyin Erkan, Mehmet Boğa, Hanife Salih, Emin Barbarus, Ömer Faruk Rahman, Sarhan Sakarya","doi":"10.1177/03913988251316438","DOIUrl":"https://doi.org/10.1177/03913988251316438","url":null,"abstract":"<p><p>Polytetrafluoroethylene (PTFE) grafts are of great importance for vascular surgery and many methods have been developed to improve their biocompatibility. The most important of these methods is the coating of the inner surfaces of the grafts. In this study, the effects of surface coatings used in vascular grafts on endothelialization and bacterial biofilm formation were investigated. Three different PTFE graft types, heparin coated, carbon coated and uncoated, were compared. HUVEC cell culture was used for endothelialization experiments and Staphylococcus aureus strain was used for biofilm formation. Endothelialization was evaluated by inverted microscopy and scanning electron microscopy (SEM). Heparin-coated grafts showed more biofilm formation than other graft types (<i>p</i> < 0.01). Moderate biofilm formation was observed in carbon-coated grafts (<i>p</i> < 0.05). When evaluating endothelialization, heparin-coated grafts showed more cell adhesion in the first days, but lagged behind the other graft types in the following days. Carbon-coated grafts showed more endothelial cell proliferation in the long term. While biofilm formation was high in heparin-coated grafts, carbon-coated grafts provided better endothelialization. Our study showed that the coating of PTFE grafts significantly affects biocompatibility and infection risk.</p>","PeriodicalId":13932,"journal":{"name":"International Journal of Artificial Organs","volume":" ","pages":"3913988251316438"},"PeriodicalIF":1.4,"publicationDate":"2025-02-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143189293","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Collagen as a biomaterial for skin wound healing: From structural characteristics to the production of devices for tissue engineering.
IF 1.4 4区 医学
International Journal of Artificial Organs Pub Date : 2025-02-02 DOI: 10.1177/03913988251316437
Luciana P Giorno, Sonia M Malmonge, Arnaldo R Santos
{"title":"Collagen as a biomaterial for skin wound healing: From structural characteristics to the production of devices for tissue engineering.","authors":"Luciana P Giorno, Sonia M Malmonge, Arnaldo R Santos","doi":"10.1177/03913988251316437","DOIUrl":"10.1177/03913988251316437","url":null,"abstract":"<p><p>Collagen is an abundant component in the human body and plays a fundamental role in the integrity and function of various tissues, including skin, bones, joints, and connective tissues. This natural polymer also contributes to physiological balance and individual health. Within this context, this article reviews the structure of collagen, describing intrinsic characteristics that range from its molecular composition to its organization into bundles. Additionally, the review highlights some of the applications of collagen in tissue engineering, particularly its mimicry of the skin's extracellular matrix. For this review, searches were performed in PubMed, Scopus, and Web of Sciences. The inclusion criteria were established based on the relevance of the studies for the objectives of the review and methodological quality. After selection of the articles, a critical analysis of their content was conducted and the information was synthesized and presented concisely. Analysis of the properties of collagen revealed its key importance for the design of bioactive materials in regenerative applications. However, challenges such as the need for improvement of the integration of implanted materials and a better understanding of the underlying biological processes remain.</p>","PeriodicalId":13932,"journal":{"name":"International Journal of Artificial Organs","volume":" ","pages":"3913988251316437"},"PeriodicalIF":1.4,"publicationDate":"2025-02-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143079784","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Evaluation of warfarin dose and INR time in therapeutic range in left ventricular assist device patients with sleeve gastrectomy.
IF 1.4 4区 医学
International Journal of Artificial Organs Pub Date : 2025-02-01 Epub Date: 2025-02-16 DOI: 10.1177/03913988251317820
Stephanie Robertson, Phillip Weeks, Elaine Chow, Brian Gulbis, Sriram Nathan, Maria Patarroyo-Aponte, Igor Gregoric, Shinil K Shah, Kulvinder S Bajwa, Biswajit Kar
{"title":"Evaluation of warfarin dose and INR time in therapeutic range in left ventricular assist device patients with sleeve gastrectomy.","authors":"Stephanie Robertson, Phillip Weeks, Elaine Chow, Brian Gulbis, Sriram Nathan, Maria Patarroyo-Aponte, Igor Gregoric, Shinil K Shah, Kulvinder S Bajwa, Biswajit Kar","doi":"10.1177/03913988251317820","DOIUrl":"10.1177/03913988251317820","url":null,"abstract":"<p><p>Sleeve gastrectomy is safe and effective in patients with left ventricular assist devices (LVADs) and morbid obesity to improve candidacy for transplantation and increase survival rates. Literature describing warfarin anticoagulation in this population is limited. A single-center, propensity score-matched, retrospective cohort study was conducted to determine if sleeve gastrectomy in LVAD-implanted patients has an effect on warfarin dose requirements in the outpatient setting. Patients were eligible for inclusion if they were 18 years of age or older, underwent LVAD implant at the study center, and were discharged from the hospital on warfarin therapy. They must have at least 8 weeks of available follow-up data post-discharge. Propensity matching was utilized to identify a non-sleeve gastrectomy LVAD-only patients for comparison. A total of 96 LVAD-only patients and 48 LVAD plus sleeve gastrectomy patients were included in the final analysis. Outpatient warfarin requirements increased from baseline over time in both groups, with no significant differences between groups except at month 12, with a mean total weekly dose of 38.1 ± 21.4 mg in the LVAD only group and 46.8 ± 18.6 mg in the LVAD with sleeve gastrectomy group (<i>p</i> = 0.05). The sleeve gastrectomy group had a significantly lower warfarin doses per kilogram of body weight until month 6 post-discharge. The percent time in therapeutic range was significantly lower in the SG group at the 8 week, 3 month, and 6 month interval time point. There were no significant differences in the incidence of bleeding or thromboembolic events.</p>","PeriodicalId":13932,"journal":{"name":"International Journal of Artificial Organs","volume":" ","pages":"77-83"},"PeriodicalIF":1.4,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143433178","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Impact of hospital volume on in-hospital outcomes for patients undergoing extracorporeal membrane oxygenation post-cardiac surgery: Evidence from nationwide inpatient sample.
IF 1.4 4区 医学
International Journal of Artificial Organs Pub Date : 2025-02-01 Epub Date: 2025-02-14 DOI: 10.1177/03913988251313886
Fei Yang, Hui Shi, Shaohua Wang, Xiaoqi Wang, Yunying Wang, Xiaoli Zhao, Liu Yang, Lingling Wang, Jingjing Zhang, Pan Pan
{"title":"Impact of hospital volume on in-hospital outcomes for patients undergoing extracorporeal membrane oxygenation post-cardiac surgery: Evidence from nationwide inpatient sample.","authors":"Fei Yang, Hui Shi, Shaohua Wang, Xiaoqi Wang, Yunying Wang, Xiaoli Zhao, Liu Yang, Lingling Wang, Jingjing Zhang, Pan Pan","doi":"10.1177/03913988251313886","DOIUrl":"10.1177/03913988251313886","url":null,"abstract":"<p><strong>Objective: </strong>This study aimed to determine the impact of hospitals' extracorporeal membrane oxygenation (ECMO) procedure volume on inpatient outcomes in patients receiving ECMO after cardiac surgery.</p><p><strong>Methods: </strong>The records of patients in the United States Nationwide Inpatient Sample database ⩾18 years old who underwent cardiac surgery and received ECMO postoperatively from 2005 to 2020 were retrospectively analyzed. Associations between hospital ECMO volume, demographical and clinical variables, and in-hospital mortality, non-routine discharge, hospital costs, acute organ failure, and infection/sepsis were examined.</p><p><strong>Results: </strong>Data of 1465 patients hospitalized in 892 hospitals were analyzed. There were 102 high ECMO-volume hospitals and 790 low ECMO-volume hospitals. Patients treated in high ECMO-volume hospitals (<i>n</i> = 317) had a significantly decreased risk of in-hospital mortality (adjusted OR (aOR) = 0.69, 95% confidence interval (CI): 0.51-0.94) compared to those treated in low ECMO-volume hospitals (<i>n</i> = 1148). In contrast, patients treated in high-volume hospitals had a significantly increased risk of non-routine discharge (aOR = 1.52, 95% CI: 1.03-2.25, <i>p</i> = 0.034) than those who stayed in the low-volume hospitals.</p><p><strong>Conclusions: </strong>High hospital ECMO volume is associated with a lower risk of in-hospital death among patients receiving ECMO after cardiac surgeries, indicating the need for policies that guide patient referrals to institutions with more extensive ECMO experience.</p>","PeriodicalId":13932,"journal":{"name":"International Journal of Artificial Organs","volume":" ","pages":"92-104"},"PeriodicalIF":1.4,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143425449","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Study on the effectiveness and safety of artificial intermittent infusion hemodiafiltration in MHD patients with intradialytic hypotension. 人工间歇输注血液滤过治疗MHD伴溶性低血压的有效性和安全性研究。
IF 1.4 4区 医学
International Journal of Artificial Organs Pub Date : 2025-02-01 Epub Date: 2025-01-08 DOI: 10.1177/03913988241310985
YanNa Fan, Fen Wang, Menglin Zou, Laimin Luo, Ying Wang
{"title":"Study on the effectiveness and safety of artificial intermittent infusion hemodiafiltration in MHD patients with intradialytic hypotension.","authors":"YanNa Fan, Fen Wang, Menglin Zou, Laimin Luo, Ying Wang","doi":"10.1177/03913988241310985","DOIUrl":"10.1177/03913988241310985","url":null,"abstract":"<p><strong>Objective: </strong>To evaluate the effectiveness and safety of artificial intermittent infusion hemodiafiltration (I-HDF) in maintenance hemodialysis (MHD) patients with intradialytic hypotension (IDH), and to determine the optimal infusion dosage.</p><p><strong>Methods: </strong>This single-center, prospective, self-controlled study included 30 MHD patients with IDH, treated from December 2022 to July 2023. Patients underwent three sessions of I-HDF as treatment group and conventional hemodialysis as control group. Comparisons were made between the two groups regarding changes in blood pressure, hypotension symptoms, changes in body water content, and achievement of infusion doses.</p><p><strong>Results: </strong>1. The fluctuation amplitude of SBP in the treatment group was 18.96 ± 10.400, while in the control group it was 27.4 ± 11.796. There was a significant difference between the two groups (<i>p</i> < 0.05). 2. During 90 sessions of dialysis, 39 interventions were needed in the treatment group, compared to 59 interventions in the control group. The treatment group required fewer interventions, with a significant difference (<i>p</i> < 0.05). 3. No hypotension symptoms occurred in the treatment group, whereas six cases were observed in the control group, which was significantly higher (<i>p</i> < 0.05). 4. One patient in the 250 ml infusion group experienced chest tightness. Among the three infusion groups, the 250 ml group had the greatest fluctuation in DBP, with a significant difference (<i>p</i> < 0.05). 5. Among the three infusion volumes groups, there was a significant difference between the theoretical and actual infusion volumes in the 150 and 200 ml groups (<i>p</i> < 0.05).</p><p><strong>Conclusion: </strong>The artificial I-HDF mode effectively improves the occurrence of IDH. An infusion dose of 150-200 ml is deemed appropriate.</p>","PeriodicalId":13932,"journal":{"name":"International Journal of Artificial Organs","volume":" ","pages":"69-76"},"PeriodicalIF":1.4,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142948356","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
The effect of early rehabilitation on therapeutic outcomes in ICU patients on mechanical ventilation: A meta-analysis. 早期康复对ICU机械通气患者治疗结果的影响:一项meta分析。
IF 1.4 4区 医学
International Journal of Artificial Organs Pub Date : 2025-02-01 Epub Date: 2025-01-08 DOI: 10.1177/03913988241311816
Saisai Li, Xiulu Xu, Jingjing Hu, Yuexia Wang
{"title":"The effect of early rehabilitation on therapeutic outcomes in ICU patients on mechanical ventilation: A meta-analysis.","authors":"Saisai Li, Xiulu Xu, Jingjing Hu, Yuexia Wang","doi":"10.1177/03913988241311816","DOIUrl":"10.1177/03913988241311816","url":null,"abstract":"<p><strong>Objective: </strong>To evaluate the effect of early rehabilitation on therapeutic outcomes of patients in the ICU requiring mechanical ventilation.</p><p><strong>Methods: </strong>Electronic databases up to June 15, 2024 were searched. Randomized controlled trials (RCTs) that compared early rehabilitation with standard rehabilitation for patients in the ICU on mechanical ventilation were included. The effects of early rehabilitation on outcomes such as duration of mechanical ventilation (days), ICU length of stay (days), hospital length of stay (days), ICU and in-hospital mortality, and ICU-acquired weakness (ICU-AW) were evaluated using a random-effects model.</p><p><strong>Results: </strong>Nineteen RCTs met the inclusion criteria for this study, involving 3076 patients in the ICU on mechanical ventilation. Meta-analysis based on the random-effects model showed that early rehabilitation significantly reduced the duration of mechanical ventilation, ICU-AW risk, ICU length of stay, and total hospital length of stay. Analysis of the timing of early rehabilitation indicated that implementing early rehabilitation within ⩽48 or ⩽72 h after ICU admission or mechanical ventilation had varying effects on the duration of mechanical ventilation, ICU length of stay, and total hospital length of stay.</p><p><strong>Conclusion: </strong>Early rehabilitation can improve the therapeutic outcomes for ICU patients on mechanical ventilation. The optimal time for implementing early rehabilitation appears to be 48-72 h after ICU admission or initiation of mechanical ventilation, but further research is needed.</p><p><strong>Clinical trial number: </strong>INPLASY202470068.</p>","PeriodicalId":13932,"journal":{"name":"International Journal of Artificial Organs","volume":" ","pages":"105-122"},"PeriodicalIF":1.4,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142948439","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Comparison of the use of standard heparin and 0.9% saline as lock solution in hemodialysis catheter: Importance of closure technique. 标准肝素与0.9%生理盐水作为血液透析导管锁膜液的比较:锁膜技术的重要性。
IF 1.4 4区 医学
International Journal of Artificial Organs Pub Date : 2025-02-01 Epub Date: 2025-01-03 DOI: 10.1177/03913988241310759
Fatih Ergül, Mikail Dağ, Beyza Doğan, Edip Erkuş, Ibrahim Guney, Süleyman Karaköse
{"title":"Comparison of the use of standard heparin and 0.9% saline as lock solution in hemodialysis catheter: Importance of closure technique.","authors":"Fatih Ergül, Mikail Dağ, Beyza Doğan, Edip Erkuş, Ibrahim Guney, Süleyman Karaköse","doi":"10.1177/03913988241310759","DOIUrl":"10.1177/03913988241310759","url":null,"abstract":"<p><strong>Background: </strong>Bleeding and thrombotic occlusion are complications of Central venous catheters. When selecting a catheter lock solution, factors such as bleeding, thrombotic occlusion, infection, and cost-effectiveness must be considered.</p><p><strong>Methods: </strong>The study included 35 patients who used heparin as a locking solution and 35 patients who used 0.9% saline, retrospectively. In our center, after injecting the solution equal to the catheter lumen volume from the syringe, approximately 1 cc of locking solution is left in the syringe, the catheter is locked and the syringe is removed from the catheter after locking. Intergroup complications were observed.</p><p><strong>Results: </strong>No significant difference was observed between the groups concerning the catheter insertion site (<i>p</i> = 0.143 and 0.143). Additionally, no significant differences were found between the groups in terms of thrombosis and bleeding complications (<i>p</i> = 0.314 and 0.239, respectively).</p><p><strong>Conclusion: </strong>Our study concluded that heparin locking is not superior to normal saline locking in terms of catheter dysfunction. We aimed to emphasize that the technique of catheter locking is more important than the type of lock solution used.</p>","PeriodicalId":13932,"journal":{"name":"International Journal of Artificial Organs","volume":" ","pages":"64-68"},"PeriodicalIF":1.4,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142926892","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
The pattern of cytokine profile in children received extracorporeal blood purification.
IF 1.4 4区 医学
International Journal of Artificial Organs Pub Date : 2025-02-01 Epub Date: 2025-01-24 DOI: 10.1177/03913988251313885
Wun Fung Hui, Renee Wan Yi Chan, Chun Kwok Wong, Wing Lum Cheung, Shu Wing Ku, Kam Lun Hon
{"title":"The pattern of cytokine profile in children received extracorporeal blood purification.","authors":"Wun Fung Hui, Renee Wan Yi Chan, Chun Kwok Wong, Wing Lum Cheung, Shu Wing Ku, Kam Lun Hon","doi":"10.1177/03913988251313885","DOIUrl":"10.1177/03913988251313885","url":null,"abstract":"<p><p>We reported the cytokine profile in children <18 years old who received extracorporeal blood purification (EBP) for sepsis, rhabdomyolysis, hyperbilirubinaemia, acute respiratory distress syndrome or cytokine storm, and determined the factors affecting the cytokine removal kinetics. Plasma levels of 38 types of cytokine/chemokine were measured at pre-EBP, 12 and 24 h after initiating EBP. Altogether there were 11 eligible episodes admitted between April 2021 and December 2023. 72.7% were male with a median (25th, 75th percentile) age of 8.7 (5.4, 15.7) years old. The overall mortality rate was 45.5% but there was no EBP-associated mortality. EBP modalities included Cytosorb<sup>®</sup> haemoadsorption (63.6%) and Oxiris<sup>®</sup> haemodiafiltration (36.4%). Thirty-seven (97.4%) cytokines exhibited a concentration reduction following EBP, and 60.5% achieved a ≥50% concentration reduction. The median removal ratio was 35.0 (21.0, 53.7)% at 12 h and 55.0 (42.1, 83.1)% at 24 h. Survivors showed a significantly higher number of cytokines with ⩾50% removal ratio at 24 h (28 vs 7, <i>p</i> = 0.017) and better removal ratio of anti-inflammatory cytokines at 12 h (67.9% vs 0%, <i>p</i> = 0.030). A higher pre-EBP cytokine concentration and higher blood flow rate were significantly associated with better removal in 16 (42.1%) and 32 (84.2%) cytokines respectively. Our study demonstrated that both devices can safely and effectively reduce the cytokine and chemokine levels in critically ill children with various conditions.</p>","PeriodicalId":13932,"journal":{"name":"International Journal of Artificial Organs","volume":" ","pages":"123-129"},"PeriodicalIF":1.4,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143033104","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Hepatic conditioning results in better lung endothelial cell preservation under hypoxic environment in vitro.
IF 1.4 4区 医学
International Journal of Artificial Organs Pub Date : 2025-02-01 Epub Date: 2025-01-30 DOI: 10.1177/03913988251315092
Kentaro Noda, Neha Atale, Taylor Austin, David A Geller, Jorg Gerlach, Pablo G Sanchez
{"title":"Hepatic conditioning results in better lung endothelial cell preservation under hypoxic environment in vitro.","authors":"Kentaro Noda, Neha Atale, Taylor Austin, David A Geller, Jorg Gerlach, Pablo G Sanchez","doi":"10.1177/03913988251315092","DOIUrl":"10.1177/03913988251315092","url":null,"abstract":"<p><strong>Background: </strong>as we look to extend <i>ex vivo</i> lung perfusion times (EVLP) to improve preservation, the metabolic activity of the lungs will require support from other organ functions. Active functional liver support, including detoxification, synthesis, and regulation, can improve lung preservation during EVLP. This study aimed to demonstrate the effects of hepatic conditioning of the EVLP perfusate on lung endothelium, via the receptor of advanced glycation end-products (RAGE)-nuclear-factor-κB (NF-κB) signaling in vitro.</p><p><strong>Methods: </strong>we performed in vitro experiments using human lung microvascular endothelial cells (HLMVECs), human hepatocytes, and perfusate (Steen solution). Four experimental groups: 1) fresh Steen (negative controls, NC), 2) EVLP'ed Steen control, this solution collected after 12 h of EVLP of human lungs, 3) hepatocyte conditioned EVLP'ed Steen (Hep-cond.), and 4) a RAGE inhibitor added in EVLP'ed Steen (RAGE inhibitor). HLMVECs were incubated in each testing condition and exposed to hypoxia (1% O<sub>2</sub>/8% CO<sub>2</sub>) for 24 h. Media were collected to investigate NF-κB signaling and endothelial glycocalyx damage.</p><p><strong>Results: </strong>HLMVECs incubated under hypoxia in EVLP'ed Steen showed significantly upregulated NF-κB signal and endothelial damage denoted by increased glycosaminoglycans and matrix metalloproteinase-2 activity among the groups. The Hep-cond. solution significantly attenuated those findings, while the RAGE inhibitor attenuated the NF-κB signal but not endothelial glycocalyx damage.</p><p><strong>Conclusion: </strong>Our study demonstrates that hepatic function incorporated into EVLP can ameliorate pulmonary endothelial cells injury under hypoxic normothermic perfusion exposure. Our data supports the concept of incorporating other organ functions into an organ perfusion platform, to enhance lung graft preservation.</p>","PeriodicalId":13932,"journal":{"name":"International Journal of Artificial Organs","volume":" ","pages":"84-91"},"PeriodicalIF":1.4,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143065482","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Advances in understanding the effects of cardiopulmonary bypass on gut microbiota during cardiac surgery.
IF 1.4 4区 医学
International Journal of Artificial Organs Pub Date : 2025-02-01 Epub Date: 2025-01-29 DOI: 10.1177/03913988251313881
Yinchang Zhang, Wei Luo, Maomao Zhao, Yongnan Li, Xiangyang Wu
{"title":"Advances in understanding the effects of cardiopulmonary bypass on gut microbiota during cardiac surgery.","authors":"Yinchang Zhang, Wei Luo, Maomao Zhao, Yongnan Li, Xiangyang Wu","doi":"10.1177/03913988251313881","DOIUrl":"10.1177/03913988251313881","url":null,"abstract":"<p><p>Cardiopulmonary bypass (CPB) is an indispensable technique in cardiac surgery; however, its impact on gut microbiota and metabolites remains insufficiently studied. CPB may disrupt the intestinal mucosal barrier, altering the composition and function of gut microbiota, thereby triggering local immune responses and systemic inflammation, which may lead to postoperative complications. This narrative review examines relevant literature from PubMed, Web of Science, Google Scholar, and CNKI databases over the past decade. Keywords such as \"gut microbiota,\" \"cardiopulmonary bypass,\" \"cardiac surgery,\" and \"postoperative complications\" were employed, with Boolean operators used to refine the search results. The review examines changes in gut microbiota before and after CPB, their role in postoperative complications, and potential strategies for modulation to improve outcomes.</p>","PeriodicalId":13932,"journal":{"name":"International Journal of Artificial Organs","volume":" ","pages":"51-63"},"PeriodicalIF":1.4,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143058963","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
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