International Journal of Artificial Organs最新文献

An innovative model of en bloc liver-pancreas-kidney preservation via ex vivo hypothermic acellular machine perfusion. 体外低温脱细胞机器灌注肝-胰-肾整体保存的创新模型。

IF 1.4 4区医学

International Journal of Artificial Organs Pub Date : 2025-05-24 DOI: 10.1177/03913988251330084

Ruy J Cruz, Flavio Fh Galvao, Anderson Benicio

{"title":"An innovative model of en bloc liver-pancreas-kidney preservation via ex vivo hypothermic acellular machine perfusion.","authors":"Ruy J Cruz, Flavio Fh Galvao, Anderson Benicio","doi":"10.1177/03913988251330084","DOIUrl":"https://doi.org/10.1177/03913988251330084","url":null,"abstract":"Background: Liver machine perfusion (MP) has emerged as a promising organ preservation modality. Recent studies have shown that the addition of the kidneys to the circuit improves the biochemical environment and could benefit liver preservation. The aim of this study was to explore the technical and anatomical feasibility of en bloc liver-pancreas-kidney MP. We also examined the safety of ex vivo perfusion with a nonoxygen carrier solution and its effects on acid-base and metabolic parameters using this novel multivisceral perfusion platform.Methods: Five multivisceral allografts, including liver, pancreas, duodenum, and kidney, were perfused for 4 h with acellular perfusate. Hemodynamic and laboratory data were evaluated throughout the experiment.Results: No system failure was reported. There were minimal changes in the acid-base parameters during the experiment. Lactate and glucose levels were stable throughout hypothermic perfusion. There was a mild increase in liver function parameters in the last hour of hypothermic perfusion. No changes in creatinine levels were observed throughout the study. The urine output increased steadily during the experiment, with an average of 155.6 mL/h.Conclusion: We described an innovative multivisceral MP technique that could be further used as a platform for physiological studies and targeted therapeutic interventions. Further investigations are necessary to evaluate this ex vivo perfusion technique and provide insights into the feasibility of hypothermic acellular multivisceral MP in clinical scenarios.","PeriodicalId":13932,"journal":{"name":"International Journal of Artificial Organs","volume":" ","pages":"3913988251330084"},"PeriodicalIF":1.4,"publicationDate":"2025-05-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144136036","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Change in plasma electrolyte concentrations during hemodialysis following a controlled step-up in dialysate bicarbonate concentration. 血液透析过程中血浆电解质浓度的变化，透析液碳酸氢盐浓度可控升高。

IF 1.4 4区医学

International Journal of Artificial Organs Pub Date : 2025-05-22 DOI: 10.1177/03913988251337323

Malgorzata Debowska, Monika Wieliczko, Mauro Pietribiasi, Urszula Bialonczyk, Jolanta Malyszko, John K Leypoldt, Jacek Waniewski

{"title":"Change in plasma electrolyte concentrations during hemodialysis following a controlled step-up in dialysate bicarbonate concentration.","authors":"Malgorzata Debowska, Monika Wieliczko, Mauro Pietribiasi, Urszula Bialonczyk, Jolanta Malyszko, John K Leypoldt, Jacek Waniewski","doi":"10.1177/03913988251337323","DOIUrl":"https://doi.org/10.1177/03913988251337323","url":null,"abstract":"Background: The concentration of bicarbonate in dialysis fluid (Dbic) that regulates acid-base balance in dialyzed patients is individually adjusted and may be modified during a hemodialysis session. We evaluated the impact of modifications of Dbic on plasma electrolytes.Methods: Two midweek hemodialysis sessions were monitored in 25 anuric, prevalent hemodialysis patients (67.9 ± 9.3 years old, 32% females). During the first session (treatment A) Dbic was constant at 33.6 ± 1.7 mmol/L, while for the second week (treatment B) it was on average 30.8 ± 2.3 mmol/L for the initial 2 h and 34.0 ± 2.5 mmol/L for the last 2 h.Results: During treatments A and B plasma bicarbonate (Pbic) increased during the sessions and was lower for treatment B than A at 60 and 120 min of dialysis. Plasma chloride decreased during treatments A and B and was significantly higher at 60 and 120 min of dialysis in treatment B than in treatment A. An increase of plasma calcium was observed during both treatments and it was higher for treatment B than A at 60 and 120 min. A similar profile of plasma sodium and potassium was observed during treatments A and B. The difference in Pbic correlated positively, whereas the differences in plasma chloride and calcium correlated negatively, with the difference in Dbic between treatments B and A.Conclusion: Modifications of dialysate bicarbonate may influence the concentrations of some other electrolytes in plasma; for each percent of Dbic increase one may expect -0.27 and -0.16% change in plasma chloride and calcium, respectively.","PeriodicalId":13932,"journal":{"name":"International Journal of Artificial Organs","volume":" ","pages":"3913988251337323"},"PeriodicalIF":1.4,"publicationDate":"2025-05-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144119700","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Dual concomitant CytoSorb hemoadsorption therapy in severe rhabdomyolysis: A novel approach to myoglobin clearance and organ preservation. 双重联合CytoSorb血液吸附治疗严重横纹肌溶解：肌红蛋白清除和器官保存的新方法。

IF 1.4 4区医学

International Journal of Artificial Organs Pub Date : 2025-05-16 DOI: 10.1177/03913988251339080

Marina Pieri, Giada Russo, Claudia Francescon, Evgeny Fominskiy, Pasquale Nardelli, Maria Grazia Calabrò, Annalisa Franco, Sara Fiorito, Silvia Ajello, Anna Mara Scandroglio

{"title":"Dual concomitant CytoSorb hemoadsorption therapy in severe rhabdomyolysis: A novel approach to myoglobin clearance and organ preservation.","authors":"Marina Pieri, Giada Russo, Claudia Francescon, Evgeny Fominskiy, Pasquale Nardelli, Maria Grazia Calabrò, Annalisa Franco, Sara Fiorito, Silvia Ajello, Anna Mara Scandroglio","doi":"10.1177/03913988251339080","DOIUrl":"https://doi.org/10.1177/03913988251339080","url":null,"abstract":"Severe rhabdomyolysis, characterized by extensive muscle breakdown and release of myoglobin and creatine kinase (CK), is a life-threatening condition often complicated by acute kidney injury (AKI) and multi-organ failure (MOF). Even when conventional treatments such as fluid resuscitation and renal replacement therapy (RRT) are timely applied, severe cases remain challenging to manage. Among therapies available in this setting, hemadsorption with CytoSorb has the potential not only to treat rhabdomyolysis through removal of circulating molecules but also to limit or even prevent rhabdomyolysis-related renal failure and MOF. In this case series we present a preliminary experience with a novel use CytoSorb hemoadsorption therapy, which encompassed the use of two CytoSorb cartridges running in parallel, to enhance myoglobin and cytokine clearance. Clinical data from the three patients with severe rhabdomyolysis treated with dual concomitant CytoSorb treatments highlighted marked improvements in CK, renal, hepatic, and inflammatory markers, with possible remarkable impact in containing rhabdomyolysis-related organ failure and death. Further investigation is warranted to establish the opportunity of tailored protocols for critically ill patients.","PeriodicalId":13932,"journal":{"name":"International Journal of Artificial Organs","volume":" ","pages":"3913988251339080"},"PeriodicalIF":1.4,"publicationDate":"2025-05-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144077851","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Enhancing the precision of in vitro hemolysis testing for blood pumps: A review. 提高血泵体外溶血检测精度的研究进展。

IF 1.4 4区医学

International Journal of Artificial Organs Pub Date : 2025-05-16 DOI: 10.1177/03913988251338141

Xu Mei, Liudi Zhang

{"title":"Enhancing the precision of in vitro hemolysis testing for blood pumps: A review.","authors":"Xu Mei, Liudi Zhang","doi":"10.1177/03913988251338141","DOIUrl":"10.1177/03913988251338141","url":null,"abstract":"In vitro hemolysis, assessed through the normalized hemolysis index (NIH) and the modified hemolysis index (MIH), serves as a critical indicator of the hemocompatibility of rotary blood pump designs. Despite the widespread application of the American Society for Testing and Materials (ASTM) standards in conducting in vitro hemolysis testing, the NIH and MIH values for a specific pump can vary considerably across different research centers or even between individual tests. To reduce this variability and facilitate global comparisons of hemolysis levels, this article reviews the underlying theory, existing literature, and empirical knowledge, alongside the practices implemented at the authors' facility. The reviewed factors influencing the variability of the hemolysis index encompass the selection of blood donor species, the source and method of blood withdrawal, blood handling and regulation, the choice of anticoagulants, the configuration of the circulation loop, local flow conditions within the loop, and the measurement of plasma-free hemoglobin. Detailed justifications and recommendations for each factor within a standardized testing framework are provided. The three primary factors that may yield more reliable and universally comparable results include enhancing clinical relevance, minimizing additional blood damage, and preventing blood clot formation. By regulating the associated parameters, it is possible to minimize measurement variance even in the absence of a predictive device.","PeriodicalId":13932,"journal":{"name":"International Journal of Artificial Organs","volume":" ","pages":"3913988251338141"},"PeriodicalIF":1.4,"publicationDate":"2025-05-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144077854","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Impella 5.5 versus intra-aortic balloon pump for bridging dual organ heart-kidney transplants: Analysis of the UNOS database. Impella 5.5与主动脉内球囊泵桥接双器官心肾移植：UNOS数据库分析

IF 1.4 4区医学

International Journal of Artificial Organs Pub Date : 2025-05-16 DOI: 10.1177/03913988251334952

Athanasios Tsiouris, Gabriel A Hernandez, Seth T Lirette, Ashok Kumar Coimbatore Jeyakumar, Lampros Papadimitriou

{"title":"Impella 5.5 versus intra-aortic balloon pump for bridging dual organ heart-kidney transplants: Analysis of the UNOS database.","authors":"Athanasios Tsiouris, Gabriel A Hernandez, Seth T Lirette, Ashok Kumar Coimbatore Jeyakumar, Lampros Papadimitriou","doi":"10.1177/03913988251334952","DOIUrl":"https://doi.org/10.1177/03913988251334952","url":null,"abstract":"Background: There has been an exponential increase in the utilization of temporary mechanical support devices for bridging Status 2 heart transplant candidates. The aim of our study is to determine outcomes in dual organ heart/kidney recipients for patients bridged with an Impella 5.5 versus IABP.Methods: We evaluated the UNOS database and analyzed Status 2 patients who underwent dual organ heart/kidney transplantation after being bridged with an Impella 5.5 or IABP from October 2019 (time of Impella 5.5 FDA approval) until March 2024.Results: A total of 457 patients who underwent Heart/Kidney transplant (HKT) were identified, of which 73% (334/457) were bridged with an IABP and 27% (123/457) with an Impella 5.5. Within the IABP cohort, 37% (122/334) were on dialysis pre-transplant compared to 43% (53/123) in the Impella group (p = 0.409). Patients in the Impella group had worse functional status, liver function, and filling pressures. There was no difference in the need for dialysis post-transplant (32% vs 29%, p = 0.613). Survival at 30, 180, and 360 days and the rates of primary graft dysfunction, acute, and chronic rejection were similar between the two groups.Conclusion: Both IABP and Impella appear to be safe for bridging dual organ heart and kidney transplant candidates, with comparable post-transplant heart and kidney graft function, complications, and survival up to 2 years. Patients receiving Impella 5.5 appear to be sicker and as a result, this percutaneous temporary mechanical circulatory support device may confer some advantages, especially for patients with dual organ failure who undergo transplantation.","PeriodicalId":13932,"journal":{"name":"International Journal of Artificial Organs","volume":" ","pages":"3913988251334952"},"PeriodicalIF":1.4,"publicationDate":"2025-05-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144077856","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A comparative study of polyethylene and silicone insoles for patient-specific conditions using computational and experimental methods. 使用计算和实验方法对患者特定条件下的聚乙烯和硅胶鞋垫进行比较研究。

IF 1.4 4区医学

International Journal of Artificial Organs Pub Date : 2025-05-16 DOI: 10.1177/03913988251334885

Doppa Govardhan, Samsundar Jayapal, Mohadese Rajaeirad, Sarah Aroujalian Mashhadi, Nima Jamshid, Ali Merdji, Raja Dhason, Sandipan Roy

{"title":"A comparative study of polyethylene and silicone insoles for patient-specific conditions using computational and experimental methods.","authors":"Doppa Govardhan, Samsundar Jayapal, Mohadese Rajaeirad, Sarah Aroujalian Mashhadi, Nima Jamshid, Ali Merdji, Raja Dhason, Sandipan Roy","doi":"10.1177/03913988251334885","DOIUrl":"https://doi.org/10.1177/03913988251334885","url":null,"abstract":"Flatfoot is a very common condition that alters gait mechanics and is usually accompanied by pain. In this current study, two types of custom-made insoles using polyethylene and silicon, respectively, were designed to help alleviate this condition. Such insoles are tested for their functionality using advanced 3D printing and finite element analysis. Tests were conducted at body weights of 60, 80, 100, and 120 kg. Results indicated that silicone insoles outperformed the others by effecting a better redistribution of pressure with higher magnitudes of strain and stress. Specifically, silicon had strain values between 1.44 × 10-7 and 2.88 × 10-7, much lower than polyethylene's 5.92 × 10-5-1.18 × 10-4. Whereby, silicon would withstand stress levels to about 47,058 Pa, while polyethylene would do so at 31,932 Pa, making it more resilient under higher loads. Further validation through kinematic analysis proved that silicon insoles enhance the symmetry of walk and disperses the concentration of pressures of the feet, therefore providing more comfort and support during locomotion. These results suggest that silicon insoles offer significant benefits for managing flatfoot, paving the way for future innovations in personalized orthopedic footwear.","PeriodicalId":13932,"journal":{"name":"International Journal of Artificial Organs","volume":" ","pages":"3913988251334885"},"PeriodicalIF":1.4,"publicationDate":"2025-05-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144077807","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Utility of a water-sparing approach for reducing dialysate flow rates in hospitalized patients with kidney failure. 节水方法降低住院肾衰竭患者透析液流速的效用。

IF 1.4 4区医学

International Journal of Artificial Organs Pub Date : 2025-05-05 DOI: 10.1177/03913988251337228

Amay Parikh, Kirsten Drake, Craig Woda, Cynthia D'Alessandri-Silva, Michael Aragon

{"title":"Utility of a water-sparing approach for reducing dialysate flow rates in hospitalized patients with kidney failure.","authors":"Amay Parikh, Kirsten Drake, Craig Woda, Cynthia D'Alessandri-Silva, Michael Aragon","doi":"10.1177/03913988251337228","DOIUrl":"https://doi.org/10.1177/03913988251337228","url":null,"abstract":"Introduction: Hemodialysis (HD) represents a necessary medical intervention for patients with end stage kidney disease (ESKD) with a high carbon footprint that significantly consumes natural resources (i.e. water). Reduction of dialysate flow rate is one strategy that directly targets water waste during HD.Methods: A retrospective multicenter, observational study was conducted over 12 months in patients with AKI-D and ESKD to evaluate treatments with mid-range dialysate flow rates set at Qd 300 mL/min (Green HD) using the Tablo® HD System (Outset Medical) versus flow rates ⩾Qd 500 mL/min (Conventional systems).Results: One thousand one hundred ninety-five treatments were performed in 433 patients using Green HD (Qd = 300 mL/min, n = 575) and Conventional systems (Qd ⩾500 mL/min, n = 620). Mean Qd for Green HD was 300.0 ± 0.0 and mean Qd for the Conventional systems was 686.6 ± 88.3 mL/min. Mean blood flow rates were significantly lower among patients treated with Green HD. Reductions in TACurea and post dialysis serum potassium were similar between the two groups. Estimates for resource utilization were lower using Green HD compared to Conventional systems.Conclusion: These findings support that higher dialysate flow rates using Conventional systems yield minimal if any benefit in small solute clearance compared to mid-range flow rates. Green HD using the Tablo® HD System results in comparable benefits in ESKD patients with significant conservation of water resources.","PeriodicalId":13932,"journal":{"name":"International Journal of Artificial Organs","volume":" ","pages":"3913988251337228"},"PeriodicalIF":1.4,"publicationDate":"2025-05-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143993226","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Contamination of microbubbles of air may occur at all investigated measurement points during hemodialysis. 在血液透析过程中，空气微泡的污染可能发生在所有被调查的测量点。

IF 1.4 4区医学

International Journal of Artificial Organs Pub Date : 2025-05-04 DOI: 10.1177/03913988251334953

Per Jonsson, Bernd Georg Stegmayr

{"title":"Contamination of microbubbles of air may occur at all investigated measurement points during hemodialysis.","authors":"Per Jonsson, Bernd Georg Stegmayr","doi":"10.1177/03913988251334953","DOIUrl":"https://doi.org/10.1177/03913988251334953","url":null,"abstract":"Microbubbles (MBs) of air occur in the hemodialysis (HD) extracorporeal circuit and may enter the bloodlines of the patient. The aim of the present study was to investigate possible sites of contamination. Seventeen patients performed 20 HD (Baxter AK200S n = 5 and Artis n = 15) and 930 ultrasound measurements of MBs/min (Hatteland CMD10 device). Detection ranges were diameters between 2.5 and 50 µm. Hemodiafiltration with postdilution (HDF-post) was performed in 14 dialyses, predilution (HDF-pre) in 1 dialysis, and HD using hemocontrol (HDhc) in 5 dialyses. Measurement points were M1-after the blood access, M2-before the dialyzer, M3-after the dialyzer, and M4-after the venous chamber. At each point, 10 measures of MBs were performed. MB contamination of the blood was larger at all points when the access was an arteriovenous fistula compared to a central dialysis catheter (p < 0.001). MB levels with the AK200 versus the Artis were lower at M1, higher at M2 (p ⩽ 0.005), and were similar at M3 and M4. HDF-pre had fewer MBs than HDF-post, whereas HDhc had more MBs than HDF-post (p < 0.001). An increase of MBs was seen at M2 during an internal \"Autotest.\" No air alarms were induced during dialyses. MBs were detected in the extra corporeal circuit at all points investigated. The venous chambers used did not significantly reduce contamination. The detected MBs did not induce air alarms when the blood returned to the patient.","PeriodicalId":13932,"journal":{"name":"International Journal of Artificial Organs","volume":" ","pages":"3913988251334953"},"PeriodicalIF":1.4,"publicationDate":"2025-05-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143964650","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The role of serum indoxyl sulfate and Klotho protein in cardiovascular complications among chronic kidney disease patients. 血清硫酸吲哚酚和Klotho蛋白在慢性肾病患者心血管并发症中的作用

IF 1.4 4区医学

International Journal of Artificial Organs Pub Date : 2025-05-01 DOI: 10.1177/03913988251334878

Qiang Liu, Qian Wang, Weina Zhang, Tiantian Jin, Beining Wang, Jie Liang, Ye Li, Si Zhang, Li Zhang, Yan Gao

{"title":"The role of serum indoxyl sulfate and Klotho protein in cardiovascular complications among chronic kidney disease patients.","authors":"Qiang Liu, Qian Wang, Weina Zhang, Tiantian Jin, Beining Wang, Jie Liang, Ye Li, Si Zhang, Li Zhang, Yan Gao","doi":"10.1177/03913988251334878","DOIUrl":"https://doi.org/10.1177/03913988251334878","url":null,"abstract":"Objective: This study aimed to investigate the relationship between the changes of serum indoxyl sulfate (IS) concentration, Klotho protein level, and cardiovascular complications in patients with chronic kidney disease (CKD) stage 3-5.Methods: A total of 108 patients with CKD stage 3-5 were selected. They were divided into three groups: CKD stage 3-4 group, CKD stage 5 non-dialysis group, and CKD stage 5 dialysis group. Echocardiography was used to measure left ventricular diameter (LVD), interventricular septal thickness (IVS), left ventricular posterior wall thickness (LVPW), and calcification.Results: there was no significant difference in age between the healthy control group and the patients with CKD stage 3-5 (p > 0.05). Compared to healthy controls, serum creatinine, serum phosphorus, iPTH, serum IS, left ventricular diameter, interventricular septum thickness, left ventricular septal wall thickness, and the proportion of valve calcification increased gradually, while serum calcium and Klotho protein decreased The level of serum IS was positively correlated with the level of LVD, IVS, and valve calcification in CKD patients, while the level of serum Klotho protein was negatively correlated with the level of IVS and valve calcification in CKD patients.Conclusion: In patients with chronic kidney disease (CKD), the incidence of cardiovascular complications is significantly higher than in the general population. The increase of serum IS level and the decrease of serum Klotho protein level are closely related to cardiac injury, it IS suggested that serum IS level and Klotho protein level may be a good index for monitoring cardiovascular injury in CKD patients.","PeriodicalId":13932,"journal":{"name":"International Journal of Artificial Organs","volume":" ","pages":"3913988251334878"},"PeriodicalIF":1.4,"publicationDate":"2025-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143978174","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Encapsulation of trabecular meshwork mesenchymal stem cell using microfluidic system for differentiation into neuron-like cells. 微流控系统包封小梁网间充质干细胞向神经元样细胞分化。

IF 1.4 4区医学

International Journal of Artificial Organs Pub Date : 2025-04-28 DOI: 10.1177/03913988251333280

Sina Rahmani, Samad Nadri, Mehdi Eskandari, Hossein Mostafavi

{"title":"Encapsulation of trabecular meshwork mesenchymal stem cell using microfluidic system for differentiation into neuron-like cells.","authors":"Sina Rahmani, Samad Nadri, Mehdi Eskandari, Hossein Mostafavi","doi":"10.1177/03913988251333280","DOIUrl":"https://doi.org/10.1177/03913988251333280","url":null,"abstract":"Objective: Neurodegenerative diseases are a class of nervous system disorders characterized by progressive neuronal degeneration and loss of function. Among emerging therapeutic approaches, microfluidic-enabled stem cell encapsulation and transplantation has gained recognition as a promising strategy for mitigating neuronal damage. In this work, human mesenchymal stem cells (MSCs), isolated from trabecular meshwork (TM) tissue, were successfully encapsulated and differentiated into neural-like cells via a microfluidic platform to demonstrate their potential for neural repair applications.Materials and methods: The isolated mesenchymal stem cells were cultured on a microfluidic system (fabricated by soft lithography methods) and treated with medium containing DMEM supplemented with RA, IBMX, and forskolin for 7 days. Quantitative PCR (qPCR) were used to analyze differentiated TM-MSC and their expression of neural-like specific markers such as Nestin and b-tubulin 3.Results: qPCR analysis revealed the presence of genes characteristic of neural cells (Nestin and β-tubulin 3) in cells differentiated both within a microfluidic system and on traditional tissue culture plates (TCPS). qPCR result showed that cells on 1.5% alginate showed higher expression of β-tubulin 3 compared to those on 1% alginate, 2% alginate, and TCPS (p < 0.0001). In contrast, Nestin expression showed no statistically significant differences across all pairwise comparisons (p > 0.05 for all groups).Conclusion: The findings indicate that mesenchymal stem cells derived from the trabecular meshwork (TM-MSCs) may serve as promising candidates for cell-based therapeutic strategies. Furthermore, the microfluidic platform implemented in this study exhibits potential utility as a delivery vehicle for TM-MSCs in therapeutic interventions targeting neurological disorders.","PeriodicalId":13932,"journal":{"name":"International Journal of Artificial Organs","volume":" ","pages":"3913988251333280"},"PeriodicalIF":1.4,"publicationDate":"2025-04-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143968952","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0