I. El-Araby, Sara M. Magdy, Ashraf Said, A. A. Saleh
{"title":"Polymorphisms of Growth Differentiation Factor 9 (GDF 9)and Bone Morphogenetic Protein 15 (BMP15) Genes in Barki and Rahmani Sheep Breeds","authors":"I. El-Araby, Sara M. Magdy, Ashraf Said, A. A. Saleh","doi":"10.21608/ZVJZ.2019.10071.1025","DOIUrl":"https://doi.org/10.21608/ZVJZ.2019.10071.1025","url":null,"abstract":"Blood samples from 100 ewes (Barki and Rhmani breeds, 50 each) were collected from a private farm at Giza Governorate for determination of polymorphisms of bone morphogenetic protein 15 (BMP15) and the growth differentiation factor 9 (GDF9) genes in the two breeds using PCR-RFLP and DNA sequencing.Restriction analysis of 712 bpGDF9 gene (amplicon of exon I) using HhaI enzyme revealed 120, 254 and 338 fragments without any differences between the two breeds being tested. GDF9 gene exon II amplicon(713 bp) showed similar pattern of restriction in the two tested breeds with 4 bands (54, 62, 137 and 460bp) using HinfI enzyme. The restriction enzymes, HinfI, SpeI and XbaI, failed to digest the amplicons of exons I and II(500 bp, each) of BMP15 gene in all tested animals of both breeds suggesting absence of polymorphism. This study concluded that there was no polymorphism in the four exons between Barki and Rahmani sheep and the two breeds have the wild genotype with absence of any mutation.","PeriodicalId":137314,"journal":{"name":"Zagazig Veterinary Journal","volume":"83 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2019-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"121060749","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"The Clinicopathological Effects of the Double Immunization with Formalized Killed Vaccine against Pasteurella multocida Challenge in Rabbits","authors":"M. F. Mohamed, M. Hashem, E. Mahmoud","doi":"10.21608/ZVJZ.2019.4212.1001","DOIUrl":"https://doi.org/10.21608/ZVJZ.2019.4212.1001","url":null,"abstract":"The disease caused by Pasteurella multocida is an extremely common and difficult issue of rabbits utilized for biomedical research. The vaccination techniques are dependably the most powerful preventive measures. In the present study, 30 New Zealand rabbits (1.5 kg average body weight and 6–8 weeks old) were divided into 3 groups, control group (I), challenged non-vaccinated group (II), and challenged double vaccinated group (III). 1st dose was administered at the 1st day followed by similar booster dose after 21 days. At the end of the 2nd, 4th, and 6th weeks of the experiment, blood samples were collected from the ear vein for hematological, plasma, and serum examination. At the end of the 6th week, the rabbits were anaesthetized and sacrificed to collect the tissue specimens from liver, kidneys, spleen, lungs and heart for histopathological study. The results showed that double immunization with killed vaccine of P. multocida increased the immune response of the animals and the leukocyte phagocytic activity against P. multocidaand also improved the clinicopathological and histopathological findings. Taken together, our findings proved that double immunization with killed vaccine of P. multocida increased the phagocytic activity of the immune cells and the immune status of animals against infection.","PeriodicalId":137314,"journal":{"name":"Zagazig Veterinary Journal","volume":"17 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2019-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"126521885","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Risk Assessment of some Heavy Metals from Claris gariepinus (African catfish) Consumed in Sharkia Governorate, Egypt","authors":"Eman F. Elawady, R. M. Bayomi","doi":"10.21608/ZVJZ.2019.10727.1031","DOIUrl":"https://doi.org/10.21608/ZVJZ.2019.10727.1031","url":null,"abstract":"This study was conducted to determine the residual concentrations of lead (Pb), cadmium (Cd), mercury (Hg) and Arsenic (As) in Claris gariepinus from Abou Hammad, Zagazig and Faqous fish markets at Sharkia Governorate, Egypt, to assess such metals dietary intake, and to evaluate the possible health risks linked with the fish consumption. Therefore, ninety Claris gariepinus samples, 30 of each, from Abou Hammad, Zagazig and Faqous fish markets were analyzed using atomic absorption spectrophotometer (AAS). The obtained results showed that, the mean residual concentrations in Clarias gariepinusfrom Abou Hammad, Zagazig and Faqous fish markets were 0.08±0.02, 0.19±0.02 and 0.4±0.06 ppm for Cd, respectively. While, the results of Pb were 0.45±0.07, 0.42±0.04 and 0.51±0.08 ppm, respectively; 0.59±0.15, 1.02±0.08 and 0.15±0.03 ppm for Hg, and 0.74±0.06, 0.69±0.04 and 0.64±0.08 ppm for As. The estimated metals' concentration exceeded the recommended safety limits outlined by Egyptian standards (ES No 7136/2010) for most samples. The total estimated daily intakes (EDI) of Cd, Pb, Hg and As were 1, 3.57, 0.57and 2.14 μg/kg BW/day, respectively, that were less than the tolerable daily intake (TDI) suggested by the Joint Expert Committee on Food Additives (JECFA). Referring to the potential health risks of inspected Claris gariepinus, it was valued that the target hazard quotient (THQ) of As of all Claris gariepinus samples from Abou Hammad, Zagazig and Faqous fish markets were 1.34, 1.25, 1.16, respectively. These results were more than 1, representing possible health risks, while THQs of other metals (Pb, Cd, Hg) from Claris gariepinus consumption from all examined areas were lower than 1 as compared to the reference doses. The obtained results give us an alert that the consumer could be under health hazards so that further investigation of Claris gariepinus is recommended at Sharkia Governorate.","PeriodicalId":137314,"journal":{"name":"Zagazig Veterinary Journal","volume":"12 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2019-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"128031841","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Y. Tartor, N. Gharieb, Wesam S Ali, E. El-Naenaeey, A. Ammar
{"title":"Rapid and Precise Diagnostic Tests for S. equi: An Etiologic Agent of Equine Strangles","authors":"Y. Tartor, N. Gharieb, Wesam S Ali, E. El-Naenaeey, A. Ammar","doi":"10.21608/ZVJZ.2019.9896.1024","DOIUrl":"https://doi.org/10.21608/ZVJZ.2019.9896.1024","url":null,"abstract":"Strangles is a highly infectious, worldwide, costly disease, affects the upper respiratory system of equine and is caused by Streptococcus equi. Early diagnosis ought to be performed for infected and carrier horses by rapid and accurate diagnostic methods. Bacteriological culture, Matrix assisted laser desorption ionization-time of flight mass spectrometry (MALDI–TOF MS), polymerase chain reaction (PCR) and DNA sequencing of S. equi M-protein (SeM) were the common methods for detection and differentiation of different subtypes of S. equi. In forty percent of suspected strangles cases, bacteriological culture may fail to detect S. equi. Recently, the development of direct sample PCR for estimation of S. equi in samples provides an alternative and potentially more sensitive method for diagnosis of equine strangles. This review article highlights the different methods of diagnosis, the role of chronic carrier in transmission of infection to susceptible animals and the different methods for identification and discrimination of β-haemolytic streptococci in respiratory samples of horses.","PeriodicalId":137314,"journal":{"name":"Zagazig Veterinary Journal","volume":"68 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2019-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"129199542","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
K. El-Bayomi, F. Mohamed, M. S. El-Tarabany, H. Gouda
{"title":"Application of Different Biostatistical Methods in Biological Data Analysis","authors":"K. El-Bayomi, F. Mohamed, M. S. El-Tarabany, H. Gouda","doi":"10.21608/ZVJZ.2019.11121.1034","DOIUrl":"https://doi.org/10.21608/ZVJZ.2019.11121.1034","url":null,"abstract":"Logistic regression is one of the popular methods used in genetic data analysis. That is applied to predict a categorical binary dependent variable on basis of predictor variables, and to test the probability of getting a particular value of the dependent variable that is related to the explanatory variable. The objective of this study is to highlight the crucial role of biostatistical methods in increasing the accuracy of the results in veterinary and biological practices. Statistical analysis of previously published data in the National Research Center, Dokki, Giza, Cairo, Egypt was done using SPSS version, 24 to predict hepatocellular carcinoma metastasis by knowing the genotypes, age, and gender of the patients. The genotypes and gender displayed a significant effect on metastasis (P 0.05). There are other types of data (animal breeding and production) which were analyzed by repeated measures ANOVA and principal component analysis (PCA). The repeated measures ANOVA is equivalent to normalized ANOVA, but for related, not independent groups. Data of this test was obtained from a study aimed to measure body weight of three breeds of rabbits at 4 time points 4th, 6th, 8th and 10th weeks of the experiment. The main effect of breed types of rabbits was significant (P 1 for two variables and their values were (2.768 and 1.147).","PeriodicalId":137314,"journal":{"name":"Zagazig Veterinary Journal","volume":"436 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2019-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"133175887","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
M. Dowidar, Hamad A. El-saadawy, mennatallah tarek gobran, Haytham A. Gad
{"title":"The Hypoglycemic Effects of Ginger and Garlic Administration on Induced Diabetic Rats","authors":"M. Dowidar, Hamad A. El-saadawy, mennatallah tarek gobran, Haytham A. Gad","doi":"10.21608/ZVJZ.2019.9557.1023","DOIUrl":"https://doi.org/10.21608/ZVJZ.2019.9557.1023","url":null,"abstract":"This work was designed to investigate the possible hypoglycemic effects of ginger (Zingiber officinal) and garlic (Allium sativum) administration on type 2 diabetesinduced in rats. Seventy male adult albino rats were randomly divided into seven groups of ten animals: Normal Control (Cnt), Diabetic Control (CntD), Ginger Low (GNL), Ginger High (GNH), Garlic Low (GRL), Garlic High (GRH) and a combination group (GNH+GRH). Diabetes was induced by an intraperitoneal injection of streptozotocin (STZ, 65 mg/kg of body weight) in all groups except the Cnt group. Rats were treated with ginger and garlic powders in different doses for 2 months. At the end of experiment, glycated hemoglobin (HbA1c), serum glucose, serum insulin, cholesterol, and high density lipoprotein (HDL)concentrations, low density lipoprotein (LDL), liver glycogen and glucagon levels were estimated.Expression of Glucose-6-Phosphatase and Glucokinase genes in liver samples from each group were normalized with housekeeping gene (s-actin) using reverse transcriptase real time Polymerase chain reaction. Serum insulin and HDL concentrations were significantly (P< 0.05) higherbutbody weight, fasting blood glucose, total cholesterol, LDLand HbA1clevels were significantly (P< 0.05) lower in the Cnt, GNH, GRH and GNH+GRHgroups compared to the CntD, GNL and GRL groups. Liver glycogen level was significantly (P< 0.05) higher and serum glucagon level was significantly (P< 0.05) lower in the combination group only but non significant difference was observed for the other groups. The expression of liver Glucose-6-Phosphatase gene was significantly (P< 0.05) downregulated but the Glucokinase gene was significantly (P< 0.05) upregulated in STZ diabetic rats treated withhigh doses of ginger and garlic powders. This study suggests that ginger and garlic powders can be used to ameliorate type 2 diabetes and might also help in preventing secondary diabetic complications.","PeriodicalId":137314,"journal":{"name":"Zagazig Veterinary Journal","volume":"26 3","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2019-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"114068494","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Marwa M. M. Khodary, Nasr El-deen, Ibtisam M. Gamal el-Deen
{"title":"Effect of Experimental Clostridium perfringens Infection on Some Immunological, Hematological and Biochemical Values in Broiler Chickens","authors":"Marwa M. M. Khodary, Nasr El-deen, Ibtisam M. Gamal el-Deen","doi":"10.21608/ZVJZ.2019.12216.1036","DOIUrl":"https://doi.org/10.21608/ZVJZ.2019.12216.1036","url":null,"abstract":"This study aimed to demonstrate the effect of Cl. perfringens infection on broiler chickens and compares between the effect of amoxicillin and / or organic acids on Cl. perfringens infection through the evaluation of hemogram, blood chemistry, hepato-renal functions and immune response through ELISA technique. One hundred one-day old chicks were divided into five equal groups. Group (1): negative control, Group (2): Clostridium perfringens type A (1.9×109 organism/ml) infected broilers, Group (3): infected chickens treated with amoxicillin (15 mg/kg. wt.) for 5 successive days, Group (4): chickens administered organic acids (1 mL /L water) then infected and Group (5): chickens infected and treated with both amoxicillin and organic acids. Cl. perfringens infection resulted in decreased appetite, ruffled feathers and brownish diarrhea with sudden death in some cases with a mortality rate up to 25%. Birds infected and treated with amoxicillin showed mild clinical signs with 15% mortalities. Majority of chickens supplemented with organic acids followed by Cl. perfringens infection or with organic acids and amoxicillin showed depression with a mild diarrhea and 10% mortality rate. Chickens infected with Cl. perfringens had macrocytic hypochrmic anemia, leukocytosis, heterophilia and monocytosis. In addition to, a significant decrease of total protein, albumin, phagocytic % and phagocytic index with a significant increase of total golbulins, liver enzymes activities, serum uric acid, creatinine and glucose levels. Treatment of Cl. perfringens infection with amoxicillin, organic acids alone or in combination resulted in a positive effect in treatment, ameliorating the severity of infection and a significant improvement in some immunological and biochemical parameters, the best results were observed with combination.","PeriodicalId":137314,"journal":{"name":"Zagazig Veterinary Journal","volume":"36 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2019-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"128130770","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Detection of Aflatoxins and Ochratoxin A Residues in Meat Products with Amelioration by Probiotics","authors":"M. Karmi","doi":"10.21608/ZVJZ.2019.11633.1035","DOIUrl":"https://doi.org/10.21608/ZVJZ.2019.11633.1035","url":null,"abstract":"A total of 125 samples of basterma, burger, luncheon, minced meat and kofta (25 / each) were collected from meat markets in Aswan to study the presence of aflatoxins and ochratoxin A using competitive direct enzyme linked immunosorbent assay (CD-ELISA) technique. Moreover, the role of probiotics in residues' detoxification was carried out. The detection rates of aflatoxins versus ochratoxin A residues in the examined basterma, luncheon and minced meat samples were (80% Vs 92%), (92% Vs 80%) and (76% Vs 72%) respectively. The detection rate for both toxins was the same in burger (96%) and kofta (88%) samples. It was found that Lactobacillus acidophilus could reduce the aflatoxins and ochratoxin A in experimentally spiked burger to 0.12 µg/Kg (97.2%), 0.57 µg/Kg (61%), respectively, while Saccharomyces serevisae could reduce the aflatoxins in spiked burger to 0.17 µg/Kg (96%) and ochratoxin A to 0.43 µg/Kg (71.1%). It could be concluded that meat products represent a potential source of aflatoxins and ochratoxin A for consumers and probiotics significantly decrease the aflatoxins and ochratoxin A in meat products. Monitoring of meat products for mycotoxins and use of preventive compounds should be practiced.","PeriodicalId":137314,"journal":{"name":"Zagazig Veterinary Journal","volume":"1 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2019-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"134158849","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Khalifa E. Ahmad, R. A. El-Aziz, Mahran M. Abd El-Emam
{"title":"Ameliorative Effects of Curcumin-Zinc Oxide Nanoparticles Conjugate on Cyclophosphamide-Induced Infertility in Male Rats","authors":"Khalifa E. Ahmad, R. A. El-Aziz, Mahran M. Abd El-Emam","doi":"10.21608/ZVJZ.2019.28657","DOIUrl":"https://doi.org/10.21608/ZVJZ.2019.28657","url":null,"abstract":"The present study aimed to investigate the ameliorative effects of Curcumin-ZnO NPs conjugate on testicular tissue damage induced by the anticancer cyclophosphamide. Seventy adult male albino rats weighing 150±15g were used in this study; 10 of them served as a control and 60 rats were intraperitoneal injected with cyclophosphamide at a dose of 30 mg/kg BW every other alternative day for 14 days. They were subdivided into CP group, CP + Curcumin NPs (15 mg/kg B.W), CP + ZnO NPs (10 mg/kg B.W), CP + Curcumin-ZnO NPs conjugate (10 mg/kg B.W), CP + Curcumin-ZnO NPs conjugate (15 mg/kg BW) and CP + Curcumin-ZnO NPs conjugate (20 mg/kg B.W) group. All treatments were given for 30 days. The results reported that curcumin- ZnO NPs conjugate treatment at doses of 15 and 20 mg/kg BW exhibited significant increase in sperm motility percent, sperm count, testicular antioxidant enzymes activities and serum testosterone and FSH levels and significant decrease in sperm abnormalities percent compared to cyclophosphamide treated rats. It was concluded that curcumin- ZnO NPs conjugate at doses of 15 and 20 mg/kg B.W ameliorate the testicular tissue damage induced by the anticancer cyclophosphamide.","PeriodicalId":137314,"journal":{"name":"Zagazig Veterinary Journal","volume":"60 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2019-03-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"123691766","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Economic Assessment of Various Levels of Protein and Energy in Pigeon Squabs Diet","authors":"M. Omar, F. M. Hassan, Sara E. Shahin","doi":"10.21608/ZVJZ.2019.28653","DOIUrl":"https://doi.org/10.21608/ZVJZ.2019.28653","url":null,"abstract":"This study was conducted to evaluate the effect of nine diets through the application of 3×3 randomized design which included three levels of energy (2600, 2800 and 3000 Kcal/kg) with 3 supplemental protein levels (14, 16 and 18%) on growth performance, blood parameters, carcass yields and economic efficiency of Baladi pigeon squabs from 28 days old until 6 months of age (age of sexual maturity). A total of 135 pairs of squabs were randomly and independently allocated to the nine dietary treatments, each containing 15 pairs (3 replicate of 5 pairs). The diets were formulated in mash form fortified with vitamin and mineral premix. Moreover, feed and water were provided for ad-libitum consumption. The results showed that, squabs fed higher energy and crude protein diets (3000 ME+16% CP) had higher live body weight with a coincident significant decrease of the feed intake (P<0.0001). In addition, dressing (%), breast and thigh meat yield (%) increased significantly with increasing levels of both energy and protein, where they hit the peak values in squabs fed on 3000 ME+16% CP diet. Furthermore, increasing energy levels did not affect serum proteins (albumin and globulin), urea and uric acid. However, it had a significant positive effect on serum lipids (cholesterol, triglyceride, high density lipoprotein and low density lipoprotein). In terms of economic analysis, 3000 ME+16% CP diet revealed the lowest cost per Kg live weight of birds 81.87 L.E per pair, along with the highest economic return 110.3 L.E per pair and net profit 10.43 L.E per pair. In conclusion, squabs can efficiently utilize diet containing high energy (3000 Kcal/kg) and crude protein (16%) diets, consequently, it can be used to optimize their growth performance and maintain the maximum economic return.","PeriodicalId":137314,"journal":{"name":"Zagazig Veterinary Journal","volume":"12 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2019-03-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"116997374","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}