Zygote (Cambridge, England)最新文献_第3页

The efficacy of the well of the well (WOW) culture system on development of bovine embryos in a small group and the effect of number of adjacent embryos on their development. 观察了WOW培养体系对牛小群体胚胎发育的影响及相邻胚胎数量对其发育的影响。

IF 1.7

Zygote (Cambridge, England) Pub Date : 2015-06-01 Epub Date: 2014-03-05 DOI: 10.1017/S096719941400001X

Sung-Sik Kang, Sosuke Ofuji, Kei Imai, Weiping Huang, Keisuke Koyama, Yojiro Yanagawa, Yoshiyuki Takahashi, Masashi Nagano

{"title":"The efficacy of the well of the well (WOW) culture system on development of bovine embryos in a small group and the effect of number of adjacent embryos on their development.","authors":"Sung-Sik Kang, Sosuke Ofuji, Kei Imai, Weiping Huang, Keisuke Koyama, Yojiro Yanagawa, Yoshiyuki Takahashi, Masashi Nagano","doi":"10.1017/S096719941400001X","DOIUrl":"https://doi.org/10.1017/S096719941400001X","url":null,"abstract":"The aim of the present study was to clarify the efficacy of the well of the well (WOW) culture system for a small number of embryos and the effect of number of adjacent embryos in a WOW dish on blastocyst development. In conventional droplet culture, embryos in the small-number group (5-6 embryos/droplet) showed low blastocyst development compared with a control group (25-26 embryos/droplet). However, small and large numbers of embryos (5-6 and 25 embryos, respectively) in a WOW dish showed no significant differences in cleavage, blastocyst rates, and mean cell number in blastocysts compared with the control group (25-30 embryos/droplet). In addition, the number of adjacent embryos in a WOW dish did not affect the development to blastocysts and cell number in blastocysts. In conclusion, a WOW dish can provide high and stable blastocyst development in small group culture wherever embryos are placed in microwells of the WOW dish. ","PeriodicalId":136608,"journal":{"name":"Zygote (Cambridge, England)","volume":" ","pages":"412-5"},"PeriodicalIF":1.7,"publicationDate":"2015-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1017/S096719941400001X","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"40283149","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 10

Zebrafish (Danio rerio) as a possible bioindicator of epigenetic factors present in drinking water that may affect reproductive function: is chorion an issue? 斑马鱼(Danio rerio)作为饮用水中可能影响生殖功能的表观遗传因素的可能生物指标:绒毛膜是一个问题吗?

IF 1.7

Zygote (Cambridge, England) Pub Date : 2015-06-01 Epub Date: 2014-03-05 DOI: 10.1017/S0967199414000045

M Martinez-Sales, F García-Ximénez, F J Espinós

{"title":"Zebrafish (Danio rerio) as a possible bioindicator of epigenetic factors present in drinking water that may affect reproductive function: is chorion an issue?","authors":"M Martinez-Sales, F García-Ximénez, F J Espinós","doi":"10.1017/S0967199414000045","DOIUrl":"https://doi.org/10.1017/S0967199414000045","url":null,"abstract":"Emerging organic contaminants have been monitored in stream waters, raw and finished waters and wastewater effluents. Most of these contaminants, such as epigenetic substances, have been detected at very low levels. Unfortunately, their complete monitoring and/or removal are very difficult, given the increasing presence of new contaminants and due to analytical and economic considerations. For this reason, bioindicators are used as an alternative to monitor their presence. To this end, zebrafish is being used to assess certain contaminants in water quality studies. As our long-term aim is to determine if zebrafish (Danio rerio) can be used to detect environmental epigenetic factors in drinking waters with effects on human reproduction, an initial question is whether the chorion could interfere with the possible action of epigenetic factors in two reproductive events: genital ridge formation and migration of the primordial germ cells (PGCs) to these genital ridges. In the first experiment, we attempted to partially degrade the chorion of mid blastula transition (MBT) embryos with pronase, with acceptable survival rates at 5 days post fertilisation (dpf), with the group exposed for 15 min giving the best survival results. As denuded early embryos require a specific culture medium, in the next experiment embryo survival was evaluated when they were cultured up to 5 dpf in drinking waters from six different sources. Results showed a negative effect on embryo survival at 5 dpf from several waters but not in others, thus distorting the survival outcomes. These results suggest using embryos with the chorion intact from the outset when drinking waters from different sources are to be tested. ","PeriodicalId":136608,"journal":{"name":"Zygote (Cambridge, England)","volume":" ","pages":"447-52"},"PeriodicalIF":1.7,"publicationDate":"2015-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1017/S0967199414000045","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"40286145","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 9

S100A7 in the Fallopian tube: a comparative study. 输卵管中S100A7的比较研究。

IF 1.7

Zygote (Cambridge, England) Pub Date : 2015-04-01 Epub Date: 2013-10-23 DOI: 10.1017/S0967199413000464

Juan M Teijeiro, Patricia E Marini

{"title":"S100A7 in the Fallopian tube: a comparative study.","authors":"Juan M Teijeiro, Patricia E Marini","doi":"10.1017/S0967199413000464","DOIUrl":"https://doi.org/10.1017/S0967199413000464","url":null,"abstract":"The oviduct is a dynamic organ in which final gamete maturation, fertilization and early embryo development take place. It is considered to be a sterile site; however the mechanism for sterility maintenance is still unknown. S100A7 is an anti-microbial peptide that has been reported in human reproductive tissues such as prostate, testicle, ovary, normal cervical epithelium and sperm. The current work reports the presence of S100A7 in the Fallopian tube and its localization at the apical surface of epithelial cells. For comparison, porcine S100A7 was used for antibody development and search for peptide in reproductive tissues. Although present in boar seminal vesicles and seminal plasma, S100A7 was not detected on female porcine organs. Also, in contrast with the human protein, porcine S100A7 did not show anti-microbial activity under the conditions tested. Phylogenetic analyses showed high divergence of porcine S100A7 from human, primate, bovine, ovine and equine sequences, being the murine sequence at a most distant branch. The differences in sequence homology, Escherichia coli-cidal activity, detectable presence and localization of S100A7 from human and pig, suggest that there are possible different functions in each organism. ","PeriodicalId":136608,"journal":{"name":"Zygote (Cambridge, England)","volume":" ","pages":"229-36"},"PeriodicalIF":1.7,"publicationDate":"2015-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1017/S0967199413000464","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"40260410","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 4

Ultrastructure of vitrified rabbit transgenic embryos. 玻璃化兔转基因胚胎的超微结构。

IF 1.7

Zygote (Cambridge, England) Pub Date : 2014-11-01 Epub Date: 2013-10-23 DOI: 10.1017/S0967199413000282

P Chrenek, A V Makarevich, M Popelková, J Schlarmannová, S Toporcerová, A Ostró, J Zivčák, Zs Bosze

{"title":"Ultrastructure of vitrified rabbit transgenic embryos.","authors":"P Chrenek, A V Makarevich, M Popelková, J Schlarmannová, S Toporcerová, A Ostró, J Zivčák, Zs Bosze","doi":"10.1017/S0967199413000282","DOIUrl":"https://doi.org/10.1017/S0967199413000282","url":null,"abstract":"The aim of the study was to determine the viability of rabbit transgenic (enhanced green fluorescent protein (EGFP)-positive) embryos cultured in vitro and compare with gene-microinjected (Mi) non-transgenic (EGFP-negative) embryos following vitrification. Non-microinjected and non-vitrified embryos were used as the control. Morphological signs of injury to embryo organelles were determined at the ultrastructural level using transmission electron microscopy (TEM). Morphometric evaluation was performed on cellular organelles using microphotographs obtained by TEM. Intact and Mi embryos recovered from in vivo fertilized eggs at 19-20 hours post coitum (hpc) were cultured for up to 72 hpc (morula stage), evaluated for the EGFP gene integration and then vitrified in 0.25 ml insemination straws in modified EFS (40% ethylene glycol + 18% Ficoll 70 + 0.3 M sucrose) vitrification solution. After 1-3 days the embryos were devitrified, a representative selection of embryos was analyzed by TEM and the remaining embryos were subjected to additional in vitro culture. Observations by TEM showed that the vitrified/warmed EGFP-positive and EGFP-negative embryos had a slight accumulation of cellular debris and lipid droplets compared with the control intact embryos. More severe changes were detected in the membrane structures of the treated embryos, mostly in the cytoplasmic envelope, trophoblastic microvilli, junctional contacts and mitochondria. We suggest that the higher proportion of deteriorated cell structures and organelles in the treated embryos may be due to the vitrification process rather than to mechanical violation (the gene-microinjection procedure), as a detailed inspection of ultrastructure revealed that most damage occurred in the cell membrane structures.","PeriodicalId":136608,"journal":{"name":"Zygote (Cambridge, England)","volume":" ","pages":"558-64"},"PeriodicalIF":1.7,"publicationDate":"2014-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1017/S0967199413000282","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"40261210","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 3

A role for polyglucans in a model sea urchin embryo cellular interaction. 多葡聚糖在海胆胚胎细胞相互作用中的作用。

IF 1.7

Zygote (Cambridge, England) Pub Date : 2014-08-01 Epub Date: 2013-03-27 DOI: 10.1017/S0967199413000038

Suprita Singh, Eddie Karabidian, Alexander Kandel, Stan Metzenberg, Edward J Carroll, Steven B Oppenheimer

{"title":"A role for polyglucans in a model sea urchin embryo cellular interaction.","authors":"Suprita Singh, Eddie Karabidian, Alexander Kandel, Stan Metzenberg, Edward J Carroll, Steven B Oppenheimer","doi":"10.1017/S0967199413000038","DOIUrl":"https://doi.org/10.1017/S0967199413000038","url":null,"abstract":"The enzymatic activities of commercially prepared glycosidases were verified by direct chemical assays using defined substrates and fixed and live sea urchin (Lytechinus pictus) embryos to determine if a model cellular interaction of interest to developmental biologists for over a century (interaction of archenteron tip and roof of the blastocoel) was mediated by glycans. Glycosidases (active and denatured) were incubated with microdissected archenterons and blastocoel roofs in a direct assay to learn if their enzymatic activities could prevent the normal adhesive interaction. Of the five glycosidases tested only β-amylase (an exoglycosidase) immediately inhibited the interaction at relatively low unit activity. α-Amylase (an endoglycosidase) had no measurable effect, while other glycosidases (α-glucosidase, β-glucosidase, β-galactosidase) only substantially inhibited adhesion after a 12-h incubation. We demonstrated that the five glycosidases were active (not inhibited) in the presence of embryo materials, and that cleaved sugars could be detected directly after incubation of some enzymes with the embryos. The biochemical purity of the enzymes was examined using gel electrophoresis under denaturing conditions, and the absence of contaminating proteases was confirmed using Azocoll™ substrate. As we cannot entirely rule out the presence of minor contaminating enzymatic activities, only inhibitions of adhesion after very short incubations with enzyme were considered significant and biologically relevant. Although glycans in indirect experiments have been implicated in mediating the interaction of the tip of the archenteron and roof of the blastocoel, to our knowledge, this is the first study that directly implicates polyglucans with terminal 1,4-linked glucose residues in this adhesive event.","PeriodicalId":136608,"journal":{"name":"Zygote (Cambridge, England)","volume":" ","pages":"419-29"},"PeriodicalIF":1.7,"publicationDate":"2014-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1017/S0967199413000038","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"40230404","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 5

Stages in the early and larval development of the African catfish Clarias gariepinus (Teleostei, Clariidae). 非洲鲇鱼Clarias gariepinus (Teleostei, Clariidae)的早期和幼虫发育阶段。

IF 1.7

Zygote (Cambridge, England) Pub Date : 2014-08-01 Epub Date: 2013-03-27 DOI: 10.1017/S0967199413000063

Wasiu Adekunle Olaniyi, Ofelia Galman Omitogun

{"title":"Stages in the early and larval development of the African catfish Clarias gariepinus (Teleostei, Clariidae).","authors":"Wasiu Adekunle Olaniyi, Ofelia Galman Omitogun","doi":"10.1017/S0967199413000063","DOIUrl":"https://doi.org/10.1017/S0967199413000063","url":null,"abstract":"The African catfish Clarias gariepinus Burchell 1822 is a favourite aquaculture fish in many parts of Africa and Asia because of its hardiness and fast growth rate. In this study, early, post-embryonic and larval developmental stages of C. gariepinus were examined chronologically and described. Photomicrographs of unfertilized matured oocytes from 0 min of fertilization through all cell stages to alevin, to complete yolk absorption, to free swimming larval stages are shown and documented live from lateral and top views, with the aid of a light microscope. Extruded oocytes had a mean diameter of 1 ± 0.1 mm, and possessed a thin perivitelline membrane whose space was filled with a protoplasmic layer. Heartbeat was in the range of 115-160/min prior to hatching. Hatchability rate was 85% and hatching occurred at 17 h at a controlled temperature of 28.5 ± 0.5°C, while ontogeny of the eyes and other organs were discernible. At day 4, larvae mean length was 9.3 ± 0.5 mm, exogenous feeding had commenced fully and melanophores spread cephalocaudally but were concentrated significantly on the head parts. This paper, for the first time, presents the significant chronological developmental stages of C. gariepinus embryology that will have significant implications for genetic manipulation and catfish seed production for aquaculture.","PeriodicalId":136608,"journal":{"name":"Zygote (Cambridge, England)","volume":" ","pages":"314-30"},"PeriodicalIF":1.7,"publicationDate":"2014-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1017/S0967199413000063","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"40230057","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 30

Biphasic assembly of the contractile apparatus during the first two cell division cycles in zebrafish embryos. 斑马鱼胚胎前两个细胞分裂周期中收缩器的双相装配。

IF 1.7

Zygote (Cambridge, England) Pub Date : 2014-05-01 Epub Date: 2013-03-27 DOI: 10.1017/S0967199413000051

Sarah E Webb, Cécile Goulet, Ching Man Chan, Michael Y F Yuen, Andrew L Miller

{"title":"Biphasic assembly of the contractile apparatus during the first two cell division cycles in zebrafish embryos.","authors":"Sarah E Webb, Cécile Goulet, Ching Man Chan, Michael Y F Yuen, Andrew L Miller","doi":"10.1017/S0967199413000051","DOIUrl":"https://doi.org/10.1017/S0967199413000051","url":null,"abstract":"The large and optically clear embryos of the zebrafish provide an excellent model system in which to study the dynamic assembly of the essential contractile band components, actin and myosin, via double fluorescent labelling in combination with confocal microscopy. We report the rapid appearance (i.e. within <2 min) of a restricted arc of F-actin patches along the prospective furrow plane in a central, apical region of the blastodisc cortex. These patches then fused with each other end-to-end forming multiple actin cables, which were subsequently bundled together forming an F-actin band. During this initial assembly phase, the F-actin-based structure did not elongate laterally, but was still restricted to an arc extending ~15° either side of the blastodisc apex. This initial assembly phase was then followed by an extension phase, where additional F-actin patches were added to each end of the original arc, thus extending it out to the edges of the blastodisc. The dynamics of phosphorylated myosin light chain 2 (MLC2) recruitment to this F-actin scaffold also reflect the two-phase nature of the contractile apparatus assembly. MLC2 was not associated with the initial F-actin arc, but MLC2 clusters were recruited and assembled into the extending ends of the band. We propose that the MLC2-free central region of the contractile apparatus acts to position and then extend the cleavage furrow in the correct plane, while the actomyosin ends alone generate the force required for furrow ingression. This biphasic assembly strategy may be required to successfully divide the early cells of large embryos.","PeriodicalId":136608,"journal":{"name":"Zygote (Cambridge, England)","volume":" ","pages":"218-28"},"PeriodicalIF":1.7,"publicationDate":"2014-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1017/S0967199413000051","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"40229714","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 7

Effects of cryostorage on human sperm chromatin integrity. 低温贮藏对人类精子染色质完整性的影响。

IF 1.7

Zygote (Cambridge, England) Pub Date : 2013-11-01 Epub Date: 2012-03-08 DOI: 10.1017/S0967199412000032

Adriana Fortunato, Rita Leo, Francesca Liguori

引用次数: 19

Chilling curves for Piaractus mesopotamicus (Holmberg, 1887) embryos stored at -8°C. mesopotamicus Piaractus (Holmberg, 1887)胚胎在-8°C下的冷冻曲线。

IF 1.7

Zygote (Cambridge, England) Pub Date : 2013-11-01 Epub Date: 2012-03-08 DOI: 10.1017/S0967199412000020

Taís da S Lopes, Danilo Pedro Streit, Darci Carlos Fornari, Diego de Oliveira, Ricardo Pereira Ribeiro, Elizabeth Romagosa

{"title":"Chilling curves for Piaractus mesopotamicus (Holmberg, 1887) embryos stored at -8°C.","authors":"Taís da S Lopes, Danilo Pedro Streit, Darci Carlos Fornari, Diego de Oliveira, Ricardo Pereira Ribeiro, Elizabeth Romagosa","doi":"10.1017/S0967199412000020","DOIUrl":"https://doi.org/10.1017/S0967199412000020","url":null,"abstract":"The present study investigates the effect of different slow chilling curves on the storage of pacu (Piaractus mesopotamicus) embryos submitted to chilling at -8°C. Embryos at the blastopore closure stage were divided into two groups: G1 - embryos exposed to cryoprotectant solution containing methanol (10%) and sucrose (0.5 M), treated as follows: (T1) taken directly from room temperature to the refrigerator without being submitted to the curve; (T2) chilling curve of 0.5°C/min; and (T3) chilling curve of 1°C/min; and G2 - the cryoprotectant solution alone was submitted to these same temperatures, receiving the embryos only after temperature had decreased, corresponding to treatments T4, T5 and T6, respectively. Treatments were kept at -8°C for a period of 6 h. Embryo development was evaluated for each treatment, with six replicates in an entirely randomized design. Survival among embryos not submitted to refrigeration was 94.3 ± 8.05%. Percentage of total larvae (TL) and addled eggs (AE) did not differ statistically between the groups, although percentage of swimming larvae (SL) exhibited higher values in G1 for the 1°C/min curve. Furthermore, when comparing the three chilling curves, a decrease of 1°C/min resulted in the highest TL percentage (90.85%), followed by the 0.5°C/min curve (78.52%). Thus, the use of 1°C/min chilling curves is recommended for P. mesopotamicus embryos stored for 6 h at -8°C.","PeriodicalId":136608,"journal":{"name":"Zygote (Cambridge, England)","volume":" ","pages":"345-50"},"PeriodicalIF":1.7,"publicationDate":"2013-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1017/S0967199412000020","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"40145134","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 12

Assessment of the viability of embryos stored in liquid nitrogen produced commercially using culture medium as a complementary test for stereoscopic microscopy. 利用商业培养基作为立体显微镜的补充试验，对储存在液氮中的胚胎的生存能力进行评估。

IF 1.7

Zygote (Cambridge, England) Pub Date : 2013-05-01 Epub Date: 2011-11-09 DOI: 10.1017/S0967199411000669

B Godinez, C S Galina, H Leon, M Gutierrez, N Moreno-Mendoza

{"title":"Assessment of the viability of embryos stored in liquid nitrogen produced commercially using culture medium as a complementary test for stereoscopic microscopy.","authors":"B Godinez, C S Galina, H Leon, M Gutierrez, N Moreno-Mendoza","doi":"10.1017/S0967199411000669","DOIUrl":"https://doi.org/10.1017/S0967199411000669","url":null,"abstract":"Summary The objective of the present study was to evaluate the viability of frozen embryos obtained from various private farmers in a culture medium for 4 h. Forty-seven embryos were used that had been previously graded as good and fair. These embryos were evaluated using stereoscopic microscopy by experienced clinicians prior to freezing. Embryos were divided in two groups: the non-cultured group, made up of six good quality embryos, and five fair; and the cultured group that consisted of 20 good quality embryos and 16 fair. Fifty-four per cent of the good quality embryos achieved a favourable development during culture whereas just 42% of embryos determined to be fair were observed to have adequate development. This evaluation was undertaken by serial photographs obtained at the onset of culture and 4 h later. This finding was corroborated by a more specific technique: terminal deoxynucleotide transferase dUTP nick end labelling-bromodeoxyuridine (TUNEL-BrdU). These results are indicative of the necessity of tight quality controls for commercially produced frozen embryos, as once thawed it is unlikely that clinicians will examine them to determine their physiological status prior to transfer.","PeriodicalId":136608,"journal":{"name":"Zygote (Cambridge, England)","volume":" ","pages":"110-4"},"PeriodicalIF":1.7,"publicationDate":"2013-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1017/S0967199411000669","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"40139205","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 2