Zygote (Cambridge, England)最新文献_第2页

Effect of cigarette smoke condensate on mouse embryo development and expression of pluripotency and apoptotic genes in vitro. 香烟烟雾凝聚物对小鼠胚胎发育及体外多能性和凋亡基因表达的影响。

IF 1.7

Zygote (Cambridge, England) Pub Date : 2022-12-01 Epub Date: 2022-09-06 DOI: 10.1017/S0967199421000460

Omid Banafshi, Ebrahim Mohammadi, Mohammad Abdi, Ebrahim Ghaderi, Vahideh Assadollahi, Mohammad Bagher Khadem Erfan, Mohammad Jafar Rezaei, Fardin Fathi

{"title":"Effect of cigarette smoke condensate on mouse embryo development and expression of pluripotency and apoptotic genes in vitro.","authors":"Omid Banafshi, Ebrahim Mohammadi, Mohammad Abdi, Ebrahim Ghaderi, Vahideh Assadollahi, Mohammad Bagher Khadem Erfan, Mohammad Jafar Rezaei, Fardin Fathi","doi":"10.1017/S0967199421000460","DOIUrl":"https://doi.org/10.1017/S0967199421000460","url":null,"abstract":"The aim of the present study was to investigate the effect of cigarette smoke condensate (CSC) on in vitro development of mouse embryos. In total 3000 NMRI mice 2PN embryos were divided into six groups (n = 500). The test group was exposed to 20, 40, 80, 160 or 320 μg/ml of CSC. In the control group, CSC was not added to the culture medium during the development of 2PN embryos. The effects of 20 and 80 μg/ml of CSC on genes involved in pluripotency and apoptosis, and also, the aryl hydrocarbon receptor gene was assessed in the blastocysts. Our results showed that CSC had an adverse effect on the viability of mouse embryos at the concentrations of 80, 160 and 320 μg/ml compared with the control group (P < 0.05). In contrast, it had positive effects on the viability of mouse embryos at the concentrations of 20 and 40 μg/ml compared with the control group (P < 0.05). The 20 and 80 μg/ml concentrations of CSC increased the expression of pluripotency, apoptotic, and aryl hydrocarbon receptor genes in the blastocyst embryo stage compared with the control group (P < 0.05). It can be concluded that concentrations higher than 40 μg/ml of CSC have an adverse effect on mouse embryo development in the preimplantation stages. Also, 20 and 80 μg/ml concentrations of CSC have a significant effect on the expression of pluripotency, apoptotic, and the aryl hydrocarbon receptor genes in the blastocyst embryo stage compared with the control group.","PeriodicalId":136608,"journal":{"name":"Zygote (Cambridge, England)","volume":" ","pages":"768-772"},"PeriodicalIF":1.7,"publicationDate":"2022-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"40352396","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Mitochondrial dysfunction caused by targeted deletion of Mfn1 does not result in telomere shortening in oocytes. 靶向缺失Mfn1导致的线粒体功能障碍不会导致卵母细胞端粒缩短。

IF 1.7

Zygote (Cambridge, England) Pub Date : 2022-10-01 Epub Date: 2022-06-22 DOI: 10.1017/S0967199422000089

Mauro Cozzolino, Emre Seli

{"title":"Mitochondrial dysfunction caused by targeted deletion of Mfn1 does not result in telomere shortening in oocytes.","authors":"Mauro Cozzolino, Emre Seli","doi":"10.1017/S0967199422000089","DOIUrl":"https://doi.org/10.1017/S0967199422000089","url":null,"abstract":"Telomere shortening during oocyte growth and development is related to reproductive ageing and infertility. The main mechanism involved in the maintenance of telomeres is based on telomerase activity, a specialized enzyme complex, which is capable of adding TTAGGG repeats at the ends of the chromosomes. Mitochondrial dysfunction may cause progressive shortening of telomeres by promoting the generation of reactive oxygen species. Mitofusin-1 is a protein required for mitochondrial fusion. Mice with the mitofusin-1 (Mfn1) deletion in the oocyte are characterized by accelerated follicular depletion and infertility, associated with defective oocyte maturation and follicular development. We hypothesized whether mitochondrial dysfunction in oocytes with targeted deletion of Mfn1 causes telomere shortening. We analyzed telomere length in oocyte and somatic cells in 3-, 6- and 9-month-old Mfn1-/- and wild-type mice. Immunofluorescence in oocyte mice of TRF1 and H2A.X was assessed to evaluate the interplay between the end-protection functions and the response to DNA damage occurring inside the telomeric repeats. Mitochondrial dysfunction due to the deletion of Mfn1 does not seem to affect telomere length in mouse oocytes.","PeriodicalId":136608,"journal":{"name":"Zygote (Cambridge, England)","volume":" ","pages":"735-737"},"PeriodicalIF":1.7,"publicationDate":"2022-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"40164416","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Bayesian approach for genetic analysis of production and reproduction traits in Jersey crossbred cattle. 泽西杂交牛生产和繁殖性状遗传分析的贝叶斯方法。

IF 1.7

Zygote (Cambridge, England) Pub Date : 2022-10-01 Epub Date: 2022-07-08 DOI: 10.1017/S0967199422000120

Poonam Ratwan, Manoj Kumar, Ajoy Mandal

{"title":"Bayesian approach for genetic analysis of production and reproduction traits in Jersey crossbred cattle.","authors":"Poonam Ratwan, Manoj Kumar, Ajoy Mandal","doi":"10.1017/S0967199422000120","DOIUrl":"https://doi.org/10.1017/S0967199422000120","url":null,"abstract":"The knowledge of genetic parameters of performance traits is crucial for any breeding programme in dairy animals. The present study was conducted to use a Bayesian approach for estimation of genetic parameters of production and reproduction traits in Jersey crossbred cattle. Data of Jersey crossbred cattle maintained at Eastern Regional Station, National Dairy Research Institute, West Bengal spread over a span of 41 years were utilized. The marginal posterior medians of heritability for 305-day milk yield (305MY), total milk yield (TMY), peak yield (PY), lactation length (LL), calving interval (CI), total milk yield per day of lactation length (TMY/LL) and total milk yield per day of calving interval (TMY/CI) were 0.31 ± 0.07, 0.29 ± 0.07, 0.27 ± 0.06, 0.16 ± 0.05, 0.15 ± 0.05, 0.29 ± 0.06, 0.27 ± 0.06, respectively. Moderate heritability estimates for 305MY, TMY, PY and production efficiency traits indicate the presence of adequate additive genetic variance in these traits to respond to selection combined with better herd management. Repeatability estimates for 305MY, TMY, PY, LL, CI, TMY/LL and TMY/CI were 0.57 ± 0.08, 0.58 ± 0.08, 0.51 ± 0.07, 0.34 ± 0.06, 0.31 ± 0.06, 0.54 ± 0.07 and 0.49 ± 0.07, respectively. Repeatability estimates for 305MY, TMY and PY were high in the current study, suggesting the use of first lactation records for early evaluation of Jersey crossbred cattle for future selection. Genetic correlations varied from 0.21 to 0.97 and maximum genetic correlation was observed between 305MY and TMY indicating that consideration of 305MY instead of TMY in breeding programmes would suffice. Positive genetic correlations of CI with 305MY and TMY indicated the antagonistic association between production and reproduction traits.","PeriodicalId":136608,"journal":{"name":"Zygote (Cambridge, England)","volume":" ","pages":"656-663"},"PeriodicalIF":1.7,"publicationDate":"2022-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"40491312","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Comparing the IVM laboratory outcomes between stimulated IVF with unstimulated natural cycles. 人工授精与自然授精的实验室结果比较。

IF 1.7

Zygote (Cambridge, England) Pub Date : 2022-10-01 Epub Date: 2022-06-22 DOI: 10.1017/S0967199421000885

Mohammad Ali Khalili, Mehdi Mohsenzadeh, Mojgan Karimi Zarchi, Mahboubeh Vatanparast

{"title":"Comparing the IVM laboratory outcomes between stimulated IVF with unstimulated natural cycles.","authors":"Mohammad Ali Khalili, Mehdi Mohsenzadeh, Mojgan Karimi Zarchi, Mahboubeh Vatanparast","doi":"10.1017/S0967199421000885","DOIUrl":"https://doi.org/10.1017/S0967199421000885","url":null,"abstract":"Recently, more attention has been raised towards fertility preservation in women with cancer. One option is in vitro maturation (IVM) of the immature oocytes as there is not enough time for induction of an ovarian stimulation protocol. The aim was to compare the IVM laboratory outcomes between stimulated and unstimulated (natural) in vitro fertilization (IVF) cycles. In total, 234 immature oocytes collected from 15 cancer patients who underwent an IVM programme (natural IVM) and 23 IVF cycles with a controlled ovarian hyperstimulation protocol (stimulated IVM) were analyzed. The oocyte morphology, zona pellucida (ZP), and meiotic spindle presence were measured using PolScope technology. Also, the rates of oocyte maturation and fertilization were assessed in both groups. The IVM rate was higher in the stimulated cycle (P < 0.05), but the fertilization rate was insignificant in comparison with unstimulated cycles. There were no significant differences in the spindle visualization and ZP birefringence scoring between the groups (P > 0.05). The oocyte normal morphology was better in the stimulated cycle compared with the natural cycle (P < 0.05). In conclusion, IVM can be recommended for cancer patients as an alternative treatment when there is insufficient time for conventional IVF before chemotherapy initiation.","PeriodicalId":136608,"journal":{"name":"Zygote (Cambridge, England)","volume":" ","pages":"593-599"},"PeriodicalIF":1.7,"publicationDate":"2022-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"40178969","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Comparison of sperm preparation methods to improve the recovery of mature spermatozoa in sub-fertile males. 精子制备方法的比较，以提高低生育能力雄性成熟精子的恢复。

IF 1.7

Zygote (Cambridge, England) Pub Date : 2022-10-01 Epub Date: 2022-07-08 DOI: 10.1017/S0967199422000132

Chiara Fasano, Giuseppe D'Andolfi, Loredana Di Matteo, Claudia Forte, Brian Dale, Elisabetta Tosti

{"title":"Comparison of sperm preparation methods to improve the recovery of mature spermatozoa in sub-fertile males.","authors":"Chiara Fasano, Giuseppe D'Andolfi, Loredana Di Matteo, Claudia Forte, Brian Dale, Elisabetta Tosti","doi":"10.1017/S0967199422000132","DOIUrl":"https://doi.org/10.1017/S0967199422000132","url":null,"abstract":"The integrity of chromatin in the spermatozoon is essential for reproductive outcome. The aim of this study was to evaluate the most effective and cost-effective method to reduce the percentage of spermatozoa with defects in chromatin decondensation for use in assisted reproductive technologies (ART) procedures. Sperm samples from 15 sub-fertile males were examined at CFA Naples to determine the sperm decondensation index (SDI), using the aniline blue test, before and after preparation, comparing density gradients with two different swim-up approaches. All three techniques led to a reduction in decondensed spermatozoa with no statistical difference (P > 0.05) between the control and the treated sperm. In contrast, we found a highly significant decrease in SDI (P < 0.01) after the two swim-up methods in all the samples, confirming the efficacy of these methods in lowering the percentage of chromatin compaction damage. There was no statistical difference between the two swim-up methods, however swim-up from the pellet led to improved count, motility and the percentage of normal condensed spermatozoa. We suggest that swim-up from the pellet be used in ART on sub-fertile males, both to reduce cell stress by multiple centrifugation and improve the recovery rate of mature spermatozoa.","PeriodicalId":136608,"journal":{"name":"Zygote (Cambridge, England)","volume":" ","pages":"664-673"},"PeriodicalIF":1.7,"publicationDate":"2022-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"40479596","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Can mesenchymal stem cells derived from adipose tissue and their conditioned medium improve ovarian functions? A mini-review. 来源于脂肪组织的间充质干细胞及其条件培养基能改善卵巢功能吗?本文着重。

IF 1.7

Zygote (Cambridge, England) Pub Date : 2022-10-01 Epub Date: 2022-06-22 DOI: 10.1017/S0967199422000235

Tayebe Pouryousefi-Koodehi, Shika Shayegan, Soheyla Hashemi, Reza Arefnezhad, Hossein Roghani-Shahraki, Hossein Motedayyen, Neda Taghizabet, Fatemeh Rezaei-Tazangi

引用次数: 3

Extracellular vesicles from seminal plasma improved development of in vitro-fertilized mouse embryos. 精浆细胞外囊泡促进小鼠体外受精胚胎的发育。

IF 1.7

Zygote (Cambridge, England) Pub Date : 2022-10-01 Epub Date: 2022-06-22 DOI: 10.1017/S0967199422000041

Yefei Ma, Jingyi Wang, Fang Qiao, Yongsheng Wang

{"title":"Extracellular vesicles from seminal plasma improved development of in vitro-fertilized mouse embryos.","authors":"Yefei Ma, Jingyi Wang, Fang Qiao, Yongsheng Wang","doi":"10.1017/S0967199422000041","DOIUrl":"https://doi.org/10.1017/S0967199422000041","url":null,"abstract":"In vitro fertilization (IVF) has wide application in human infertility and animal breeding. It is also used for research on reproduction, fertility and development. However, IVF embryos are still inferior to their in vivo counterparts. Some substances in seminal plasma appear to have important roles in embryo development, and during the traditional IVF procedure, the seminal plasma is washed away. In this study, extracellular vesicles (EVs) were concentrated from seminal plasma by ultracentrifugation, visualized using transmission electron microscopy, and particle size distributions and concentrations were determined with a NanoSight particle analyzer. We found particles of various sizes in the seminal plasma, the majority having diameters ranging from 100 to 200 nm and concentrations of 6.07 × 1010 ± 2.91 × 109 particles/ml. Addition of seminal plasma EVs (SP-EVs) to the IVF medium with mouse oocytes and sperm significantly increased the rate of blastocyst formation and the inner cell mass (ICM)/trophectoderm (TE) cell ratio, and reduced the apoptosis of blastocysts. Our findings provide new insights into the role of seminal plasma EVs in mediating embryo development and it suggests that SP-EVs may be used to improve the developmental competence of IVF embryos, which has important significance for assisted reproduction in animals and humans.","PeriodicalId":136608,"journal":{"name":"Zygote (Cambridge, England)","volume":" ","pages":"619-624"},"PeriodicalIF":1.7,"publicationDate":"2022-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"40178499","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Long-term cryopreservation and frozen embryo transfer do not impact clinical and neonatal outcomes: a retrospective cohort study of slow-frozen early-cleavage human embryos. 长期冷冻保存和冷冻胚胎移植不影响临床和新生儿结局:一项慢速冷冻早期卵裂人类胚胎的回顾性队列研究。

IF 1.7

Zygote (Cambridge, England) Pub Date : 2022-10-01 Epub Date: 2022-06-22 DOI: 10.1017/S0967199422000193

Biwei Shi, Long Cui, Xiaoqun Ye, Yinghui Ye

{"title":"Long-term cryopreservation and frozen embryo transfer do not impact clinical and neonatal outcomes: a retrospective cohort study of slow-frozen early-cleavage human embryos.","authors":"Biwei Shi, Long Cui, Xiaoqun Ye, Yinghui Ye","doi":"10.1017/S0967199422000193","DOIUrl":"https://doi.org/10.1017/S0967199422000193","url":null,"abstract":"This study aimed to evaluate the effect of the cryopreservation duration (up to 160 months) on the clinical and neonatal outcomes of slow-frozen early-cleavage human embryos. Clinical data collected between February 2013 and August 2017 were included in this retrospective study. Cases were classified into five groups by the duration of cryopreservation: Group 1, 6-12 months; Group 2, 13-36 months; Group 3, 37-60 months; Group 4, 61-84 months; and Group 5, >84 months. The embryo survival rate, implantation rate, clinical pregnancy rate, live-birth rate, newborn sex ratio, singleton gestational age, singleton birth weight and malformation rate were compared between the groups. The cryopreservation duration did not significantly affect the rates of clinical pregnancy (P = 0.119) and live birth (P = 0.354), the newborn sex ratio (P = 0.614) or the singleton gestational age (P = 0.212) and birthweight (P = 0.212). Although decreases in the embryo survival and implantation rates were observed in groups 4 and 5 compared with those in groups 1-3, these differences were not statistically significant (P = 0.329, P = 0.279, respectively). Long-term cryopreservation does not appear to adversely affect the clinical and neonatal outcomes of slow-frozen early-cleavage human embryos.","PeriodicalId":136608,"journal":{"name":"Zygote (Cambridge, England)","volume":" ","pages":"712-718"},"PeriodicalIF":1.7,"publicationDate":"2022-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"40164420","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 2

Morphological description of gametes in cave and surface populations of Astyanax mexicanus (De Filippi, 1853). 墨西哥Astyanax mexicanus洞穴种群和地表种群配子的形态描述(De Filippi, 1853)。

IF 1.7

Zygote (Cambridge, England) Pub Date : 2022-10-01 Epub Date: 2022-06-22 DOI: 10.1017/S0967199422000223

Víctor Rodríguez-Ballesteros, Berenit Mendoza-Garfias, Raúl Ulloa-Arvizu, Alberto Balcazar, C Patricia Ornelas-García

{"title":"Morphological description of gametes in cave and surface populations of Astyanax mexicanus (De Filippi, 1853).","authors":"Víctor Rodríguez-Ballesteros, Berenit Mendoza-Garfias, Raúl Ulloa-Arvizu, Alberto Balcazar, C Patricia Ornelas-García","doi":"10.1017/S0967199422000223","DOIUrl":"https://doi.org/10.1017/S0967199422000223","url":null,"abstract":"The Mexican tetra Astyanax mexicanus presents two contrasting morphs, a widely distributed surface morph and a cave-adapted morph. These cave-adapted morphs have evolved independently from two different lineages (i.e. 'old' and 'new' lineages); therefore, this model system gives a unique opportunity to explore parallel adaptive evolution in biological traits. The present study corresponds to the first morphological description of the Astyanax mexicanus maturation process of the spermatozoa and oocytes, using thermal and hormonal stimuli to promote spermatogenesis and oogenesis, considering surface and cave morphs from both lineages. We corroborate the relevance of thermal and hormonal stimuli to promote gamete maturation. The hormone Ovaprim (GnRHa + Domperidone) is an effective promoter of ovarian development, maturation end in oocytes and spawning in Astyanax mexicanus. The sperm morphology of Astyanax mexicanus includes the sperm head, the midpiece, and tail or flagellum. We found differences in the spermatozoan total length between environments (F = 9.929, P = 0.05) and linages (F = 49.86, P = 0.005). The oocytes showed a spherical conformation with a mean diameter of 822.4 ± 194.1 μm for the surface populations, and 604.6 ± 38.3 µm for the cave populations. The oocyte chorion presents ridges and grooves that are arranged radially towards the micropyle. A plug in the micropyle zone was observed after fertilization, confirmed by the outer membrane of the chorion, which provides some weak adhesiveness to the substrate. We observed differences in chorion thickness between the contrasting environmental conditions. This is the first morphological characterization of the Sótanos Vázquez, Escondido and Tigre, which previous to this study were only known from speleological expeditions, with no previous biological information available.","PeriodicalId":136608,"journal":{"name":"Zygote (Cambridge, England)","volume":" ","pages":"719-729"},"PeriodicalIF":1.7,"publicationDate":"2022-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"40178965","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

H1foo is essential for in vitro meiotic maturation of bovine oocytes. H1foo对牛卵母细胞的体外减数分裂成熟至关重要。

IF 1.7

Zygote (Cambridge, England) Pub Date : 2015-06-01 Epub Date: 2014-03-11 DOI: 10.1017/S0967199414000021

Yan Yun, Peng An, Jing Ning, Gui-Ming Zhao, Wen-Lin Yang, An-Min Lei

{"title":"H1foo is essential for in vitro meiotic maturation of bovine oocytes.","authors":"Yan Yun, Peng An, Jing Ning, Gui-Ming Zhao, Wen-Lin Yang, An-Min Lei","doi":"10.1017/S0967199414000021","DOIUrl":"https://doi.org/10.1017/S0967199414000021","url":null,"abstract":"Oocyte-specific linker histone, H1foo, is localized on the oocyte chromosomes during the process of meiotic maturation, and is essential for mouse oocyte maturation. Bovine H1foo has been identified, and its expression profile throughout oocyte maturation and early embryo development has been established. However, it has not been confirmed if H1foo is indispensable during bovine oocyte maturation. Effective siRNAs against H1foo were screened in HeLa cells, and then siRNA was microinjected into bovine oocytes to down-regulate H1foo expression. H1foo overexpression was achieved via mRNA injection. Reverse transcription polymerase chain reaction (RT-PCR) results indicated that H1foo was up-regulated by 200% and down-regulated by 70%. Based on the first polar body extrusion (PB1E) rate, H1foo overexpression apparently promoted meiotic progression. The knockdown of H1foo significantly impaired bovine oocyte maturation compared with H1foo overexpression and control groups (H1foo overexpression = 88.7%, H1foo siRNA = 41.2%, control = 71.2%; P < 0.05). This decrease can be rescued by co-injection of a modified H1foo mRNA that has escaped from the siRNA target. However, the H1e (somatic linker histone) overexpression had no effect on PB1E rate when compared with the control group. Therefore we concluded that H1foo is essential for bovine oocyte maturation and its overexpression stimulates the process. ","PeriodicalId":136608,"journal":{"name":"Zygote (Cambridge, England)","volume":" ","pages":"416-25"},"PeriodicalIF":1.7,"publicationDate":"2015-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1017/S0967199414000021","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"40298761","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 19